Cloned (Comment) | Organism |
---|---|
isozyme expression analysis, overview | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA knockdown of malic enzyme isozyme by 50% affects pyruvate carboxylase flux of the pyruvate derived from glutamate metabolism, insulin secretion in response to membrane depolarization using potassium chloride is unaffected by siRNA knockdown of malic enzyme, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | isozyme ME2 | Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Rattus norvegicus | mitochondrial isozyme ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting | pyruvate + CO2 + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
INS-1 832/13 cell | - |
Rattus norvegicus | - |
pancreatic beta cell | isozyme ME2 | Rattus norvegicus | - |
pancreatic islet | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
isozyme expression levels, pyruvate cycling and isozyme activity in pancreatic islets, overview | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Rattus norvegicus | pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | mitochondrial isozyme ME2 responds to elevated amino acids and serves to supply sufficient pyruvate for increased Krebs cycle flux when glucose is limiting | Rattus norvegicus | pyruvate + CO2 + NADH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NAD-malic enzyme | - |
Rattus norvegicus |
NAD-ME2 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rattus norvegicus | |
NADH | - |
Rattus norvegicus |