BRENDA - Enzyme Database
show all sequences of 1.1.1.382

Uncovering rare NADH-preferring ketol-acid reductoisomerases

Brinkmann-Chen, S.; Cahn, J.K.; Arnold, F.H.; Metab. Eng. 26C, 17-22 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Thermacetogenium phaeum
expression in Escherichia coli
uncultured archaeon
expression in Escherichia coli
Desulfococcus oleovorans
expression in Escherichia coli
Archaeoglobus fulgidus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
NADH
Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Thermacetogenium phaeum
0.005
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Archaeoglobus fulgidus
0.032
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Desulfococcus oleovorans
1.1
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
uncultured archaeon
Organism
Organism
UniProt
Commentary
Textmining
Archaeoglobus fulgidus
O28294
-
-
Desulfococcus oleovorans
-
-
-
Thermacetogenium phaeum
-
-
-
uncultured archaeon
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Thermacetogenium phaeum
-
uncultured archaeon
-
Desulfococcus oleovorans
-
Archaeoglobus fulgidus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
732457
Thermacetogenium phaeum
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
732457
uncultured archaeon
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
732457
Desulfococcus oleovorans
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
732457
Archaeoglobus fulgidus
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.1
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Archaeoglobus fulgidus
0.22
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
uncultured archaeon
0.25
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Desulfococcus oleovorans
0.46
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Thermacetogenium phaeum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Thermacetogenium phaeum
7
-
assay at
uncultured archaeon
7
-
assay at
Desulfococcus oleovorans
7
-
assay at
Archaeoglobus fulgidus
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
specific for NADH. NADH/NADPH ratio of kcat/Km is 13
Archaeoglobus fulgidus
NADH
specific for NADH. NADH/NADPH ratio of kcat/Km is 152
uncultured archaeon
NADH
specific for NADH. NADH/NADPH ratio of kcat/Km is 74
Thermacetogenium phaeum
NADH
specific for NADH. No activity with NADPH
Desulfococcus oleovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Thermacetogenium phaeum
expression in Escherichia coli
uncultured archaeon
expression in Escherichia coli
Desulfococcus oleovorans
expression in Escherichia coli
Archaeoglobus fulgidus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
specific for NADH. NADH/NADPH ratio of kcat/Km is 13
Archaeoglobus fulgidus
NADH
specific for NADH. NADH/NADPH ratio of kcat/Km is 152
uncultured archaeon
NADH
specific for NADH. NADH/NADPH ratio of kcat/Km is 74
Thermacetogenium phaeum
NADH
specific for NADH. No activity with NADPH
Desulfococcus oleovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
NADH
Km-value for NADH is below 0.001 mM, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Thermacetogenium phaeum
0.005
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Archaeoglobus fulgidus
0.032
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Desulfococcus oleovorans
1.1
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
uncultured archaeon
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermacetogenium phaeum
-
uncultured archaeon
-
Desulfococcus oleovorans
-
Archaeoglobus fulgidus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
732457
Thermacetogenium phaeum
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
732457
uncultured archaeon
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
732457
Desulfococcus oleovorans
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
(2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADH + H+
(2S)-2-hydroxy-2-methyl-3-oxobutanoate i.e. (2S)-2-acetolactate
732457
Archaeoglobus fulgidus
(2R)-2,3-dihydroxy-3-methylbutanoate + NAD+
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.1
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Archaeoglobus fulgidus
0.22
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
uncultured archaeon
0.25
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Desulfococcus oleovorans
0.46
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Thermacetogenium phaeum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Thermacetogenium phaeum
7
-
assay at
uncultured archaeon
7
-
assay at
Desulfococcus oleovorans
7
-
assay at
Archaeoglobus fulgidus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
NADH
kcat/Km for NADH is at least 460 /mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Thermacetogenium phaeum
8
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Desulfococcus oleovorans
20
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Archaeoglobus fulgidus
200
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
uncultured archaeon
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
additional information
-
NADH
kcat/Km for NADH is at least 460 /mM*s, pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Thermacetogenium phaeum
8
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Desulfococcus oleovorans
20
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
Archaeoglobus fulgidus
200
-
NADH
pH 7, temperature not specified in the publication, cosubstrate: (2S)-2-hydroxy-2-methyl-3-oxobutanoate
uncultured archaeon
Other publictions for EC 1.1.1.382
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731279
Cahn
Cofactor specificity motifs an ...
Alicyclobacillus acidocaldarius subsp. acidocaldarius, Ignisphaera aggregans, uncultured archaeon, uncultured archaeon GZfos26G2, Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446, Ignisphaera aggregans DSM 17230
Biochem. J.
468
475-484
2015
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732457
Brinkmann-Chen
Uncovering rare NADH-preferrin ...
Thermacetogenium phaeum, uncultured archaeon, Desulfococcus oleovorans, Archaeoglobus fulgidus
Metab. Eng.
26C
17-22
2014
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4
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4