Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
3-oxooctanoyl-CoA | enzyme Had, at pH 8.0 and 30°C | Cupriavidus necator | |
0.0083 | - |
3-oxohexanoyl-CoA | enzyme PaaH1, at pH 8.0 and 30°C | Cupriavidus necator | |
0.0094 | - |
3-acetoacyl-CoA | enzyme PaaH1, at pH 8.0 and 30°C | Cupriavidus necator | |
0.0125 | - |
3-oxooctanoyl-CoA | enzyme PaaH1, at pH 8.0 and 30°C | Cupriavidus necator | |
0.016 | - |
3-oxohexanoyl-CoA | enzyme Had, at pH 8.0 and 30°C | Cupriavidus necator | |
0.0175 | - |
3-acetoacyl-CoA | enzyme Had, at pH 8.0 and 30°C | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoacyl-CoA + NADH + H+ | Cupriavidus necator | - |
(S)-3-hydroxyacyl-CoA + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, HiTrap phenyl column chromatography and Superdex 200 gel filtration | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-acetoacyl-CoA + NADH + H+ | highest activity | Cupriavidus necator | (S)-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
3-oxoacyl-CoA + NADH + H+ | - |
Cupriavidus necator | (S)-3-hydroxyacyl-CoA + NAD+ | - |
? | |
3-oxohexanoyl-CoA + NADH + H+ | - |
Cupriavidus necator | (S)-3-hydroxyhexanoyl-CoA + NAD+ | - |
? | |
3-oxooctanoyl-CoA + NADH + H+ | - |
Cupriavidus necator | (S)-3-hydroxyoctanoyl-CoA + NAD+ | - |
? | |
additional information | the activity of enzymes PaaH1 and Had with NADPH is as low as 2.2% and 1.2%, respectively, of the respective activity with NADH | Cupriavidus necator | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
3HA-CoA dehydrogenase | - |
Cupriavidus necator |
HAD | - |
Cupriavidus necator |
NADH-3HB-CoA dehydrogenase | - |
Cupriavidus necator |
NADH-dependent (S)-3-hydroxyacyl-CoA dehydrogenase | - |
Cupriavidus necator |
NADH-dependent 3-hydroxyacyl-CoA dehydrogenase | - |
Cupriavidus necator |
PaaH1 | - |
Cupriavidus necator |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
3-oxooctanoyl-CoA | enzyme Had, at pH 8.0 and 30°C | Cupriavidus necator | |
40 | - |
3-oxohexanoyl-CoA | enzyme Had, at pH 8.0 and 30°C | Cupriavidus necator | |
43 | - |
3-oxohexanoyl-CoA | enzyme PaaH1, at pH 8.0 and 30°C | Cupriavidus necator | |
53 | - |
3-oxooctanoyl-CoA | enzyme PaaH1, at pH 8.0 and 30°C | Cupriavidus necator | |
84 | - |
3-acetoacyl-CoA | enzyme PaaH1, at pH 8.0 and 30°C | Cupriavidus necator | |
104 | - |
3-acetoacyl-CoA | enzyme Had, at pH 8.0 and 30°C | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Cupriavidus necator |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1700 | - |
3-oxooctanoyl-CoA | enzyme Had, at pH 8.0 and 30°C | Cupriavidus necator | |
2500 | - |
3-oxohexanoyl-CoA | enzyme Had, at pH 8.0 and 30°C | Cupriavidus necator | |
4200 | - |
3-oxooctanoyl-CoA | enzyme PaaH1, at pH 8.0 and 30°C | Cupriavidus necator | |
5200 | - |
3-oxohexanoyl-CoA | enzyme PaaH1, at pH 8.0 and 30°C | Cupriavidus necator | |
6000 | - |
3-acetoacyl-CoA | enzyme Had, at pH 8.0 and 30°C | Cupriavidus necator | |
8900 | - |
3-acetoacyl-CoA | enzyme PaaH1, at pH 8.0 and 30°C | Cupriavidus necator |