BRENDA - Enzyme Database show
show all sequences of 1.1.1.348

Crystal structure of vestitone reductase from alfalfa (Medicago sativa L.)

Shao, H.; Dixon, R.A.; Wang, X.; J. Mol. Biol. 369, 265-276 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Medicago sativa
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.0), 20% (w/v) polyethylene glycol 6000, 0.1 M MgCl2, at 4°C
Medicago sativa
Engineering
Amino acid exchange
Commentary
Organism
Y164A
the mutation abolishes enzymatic activity
Medicago sativa
Y164G
the mutation abolishes enzymatic activity
Medicago sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3R)-vestitone + NADPH + H+
Medicago sativa
-
(3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+
-
-
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago sativa
-
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-NTA agarose column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration
Medicago sativa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3R)-vestitone + NADPH + H+
-
722952
Medicago sativa
(3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+
-
-
-
ir
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Medicago sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Medicago sativa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Medicago sativa
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.0), 20% (w/v) polyethylene glycol 6000, 0.1 M MgCl2, at 4°C
Medicago sativa
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
Y164A
the mutation abolishes enzymatic activity
Medicago sativa
Y164G
the mutation abolishes enzymatic activity
Medicago sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(3R)-vestitone + NADPH + H+
Medicago sativa
-
(3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+
-
-
ir
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-NTA agarose column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration
Medicago sativa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(3R)-vestitone + NADPH + H+
-
722952
Medicago sativa
(3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+
-
-
-
ir
Other publictions for EC 1.1.1.348
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722952
Shao
Crystal structure of vestitone ...
Medicago sativa
J. Mol. Biol.
369
265-276
2007
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-
1
1
2
-
-
-
-
-
-
1
-
5
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
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-
1
1
1
2
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
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-
1
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-
-
-
-
-
-
-
-
-
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-
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-
721410
Guo
Molecular cloning and expressi ...
Medicago sativa
Arch. Biochem. Biophys.
320
353-360
1995
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
722250
Guo
The pterocarpan synthase of al ...
Medicago sativa
FEBS Lett.
356
221-225
1994
-
-
-
-
-
-
1
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
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-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
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-
1
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
722597
Guo
Conversion of vestitone to med ...
Medicago sativa
J. Biol. Chem.
269
22372-22378
1994
-
-
-
-
-
-
2
1
-
-
2
1
-
2
-
-
1
-
-
-
2
-
1
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
2
1
-
-
-
1
-
-
2
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-