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Literature summary for 1.1.1.303 extracted from

  • Muschallik, L.; Molinnus, D.; Bongaerts, J.; Pohl, M.; Wagner, T.; Schoening, M.; Siegert, P.; Selmer, T.
    (R,R)-Butane-2,3-diol dehydrogenase from Bacillus clausii DSM 8716T cloning and expression of the bdhA-gene, and initial characterization of enzyme (2017), J. Biotechnol., 258, 41-50.
    View publication on PubMed
Search for more literature for 1.1.1.303

Inhibitors

Inhibitors Comment Organism Structure
diacetyl slight substrate inhibition, physiologically irrelevant Shouchella clausii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, Arrhenius plots permit the calculation of activation energies of 27 kJ/mol, 41 kJ/mol and 60 kJ/mol for the BDH catalyzed acetoin reductase, diacetyl reductase and butane-2,3-diol dehydrogenase activities, respectively Shouchella clausii

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required Shouchella clausii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diacetyl + NADH + H+ Shouchella clausii
-
 (R)-acetoin + NAD+
-
 ir
diacetyl + NADH + H+ Shouchella clausii DSM 8716
-
 (R)-acetoin + NAD+
-
 ir

Organism

Organism UniProt Comment Textmining
Shouchella clausii A0A223LRZ7 Bacillus clausii
-
Shouchella clausii DSM 8716 A0A223LRZ7 Bacillus clausii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diacetyl + NADH + H+
-
 Shouchella clausii (R)-acetoin + NAD+
-
 ir
diacetyl + NADH + H+ stereoselective reduction Shouchella clausii (R)-acetoin + NAD+
-
 ir
diacetyl + NADH + H+
-
 Shouchella clausii DSM 8716 (R)-acetoin + NAD+
-
 ir
diacetyl + NADH + H+ stereoselective reduction Shouchella clausii DSM 8716 (R)-acetoin + NAD+
-
 ir
additional information the two step reduction of diacetyl (butane-2,3-dione) with (R,R)-BDH solely yields (R,R)-butane-2,3-diol via (R)-acetoin, cf. EC 1.1.1.4. The enzyme catalyzes the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Starting with diacetyl as a substrate, exclusively (R,R)-butane-2,3-diol is formed. Bacillus clausii BDH catalyzes a highly selective oxidation of secondary alcohol groups in (R)-configuration of butane-2,3-diols and also reduces the carbonyl function in acetoin and diacetyl in a stereoselective manner to yield the (R)-configuration of the resulting chiral center, it selectively acts on vicinal diketones, alpha-hydroxy ketones and vicinal diols Shouchella clausii ?
-
 ?
additional information the two step reduction of diacetyl (butane-2,3-dione) with (R,R)-BDH solely yields (R,R)-butane-2,3-diol via (R)-acetoin, cf. EC 1.1.1.4. The enzyme catalyzes the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Starting with diacetyl as a substrate, exclusively (R,R)-butane-2,3-diol is formed. Bacillus clausii BDH catalyzes a highly selective oxidation of secondary alcohol groups in (R)-configuration of butane-2,3-diols and also reduces the carbonyl function in acetoin and diacetyl in a stereoselective manner to yield the (R)-configuration of the resulting chiral center, it selectively acts on vicinal diketones, alpha-hydroxy ketones and vicinal diols Shouchella clausii DSM 8716 ?
-
 ?

Synonyms

Synonyms Comment Organism
(R,R)-BDH
-
 Shouchella clausii
(R,R)-butane-2,3-diol dehydrogenase
-
 Shouchella clausii
(R,R)-butanediol dehydrogenase/diacetyl reductase
-
 Shouchella clausii
BdhA
-
 Shouchella clausii
additional information see also EC 1.1.1.4 Shouchella clausii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 acetoin reductase, diacetyl reductase and butane-2,3-diol dehydrogenase activities show temperature optima at 50°C Shouchella clausii
50
-
 diacetyl reductase activity optimum Shouchella clausii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 the pH-dependence of diacetyl reduction behaves quite differently from the acetoin reductase activity (optimum pH 7.0). The pH optimum is shifted down to pH 6.0, and the pH-dependence of activity is narrower as compared to the acetoin reductase activity Shouchella clausii
6
-
 diacetyl reductase activity optimum Shouchella clausii

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Shouchella clausii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
330
-
 diacetyl pH 6.0, 50°C Shouchella clausii