Cloned (Comment) | Organism |
---|---|
gene akthr2, DNA sequence determination and analysis, located on chromosome 4, subcloning in Escherichia coli strain DH5alpha, functional complementation of the Saccharomyces cerevisiae homoserine dehydrogenase-deficient hom6 mutant strain and of the aspartate kinase-deficient hom3 mutant strain, conferring L-threonine and L-methionine prototrophy to the yeast cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Arabidopsis thaliana plants by transformation with gene akthr2 via Agrobacterium tumefaciens infection, determination of expression patterns of the gene akthr1 ans akthr2 in the transgenic plants | Arabidopsis thaliana |
additional information | mutant strain M20-20D is deficient in gene HOM6 and shows no activity, the defect can be complemented by recombinant expression of the Arabidopsis thaliana gene akthr2 in the mutant yeast cells | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-threonine | - |
Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for aspartate kinase activity | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + NADP+ | Saccharomyces cerevisiae | - |
L-aspartate 4-semialdehyde + NADPH | - |
? | |
L-homoserine + NADP+ | Arabidopsis thaliana | - |
L-aspartate 4-semialdehyde + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
var. Bensheim showing threonine and methionine prototrophy, bifunctional enzyme showing aspartate dehydrogenase and aspartate kinase activity, gene akthr2 | - |
Saccharomyces cerevisiae | - |
mutant strain M20-20D and wild-type strain S2207A | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H + H+ | bifunctional enzyme showing aspartate kinase, 2.7.2.4, and homoserine dehydrogenase activities | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
meristem | - |
Arabidopsis thaliana | - |
additional information | akthr2 is not or time-restricted expressed in stem, gynoecium, and during seed formation | Arabidopsis thaliana | - |
stamen | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + NADP+ | - |
Saccharomyces cerevisiae | L-aspartate 4-semialdehyde + NADPH | - |
? | |
L-homoserine + NADP+ | - |
Arabidopsis thaliana | L-aspartate 4-semialdehyde + NADPH | - |
? | |
L-homoserine + NADP+ | - |
Arabidopsis thaliana | L-aspartate 4-semialdehyde + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AK-HSDH | - |
Arabidopsis thaliana |
aspartate kinase-homoserine dehydrogenase | - |
Arabidopsis thaliana |
HSDH | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Saccharomyces cerevisiae | |
NADP+ | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |