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Literature summary for 1.1.1.286 extracted from

  • Miyazaki, J.; Kobashi, N.; Nishiyama, M.; Yamane, H.
    Characterization of homoisocitrate dehydrogenase involved in lysine biosynthesis of an extremely thermophilic bacterium, Thermus thermophilus HB27, and evolutionary implication of beta-decarboxylating dehydrogenase (2003), J. Biol. Chem., 278, 1864-1871.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
R85V complete loss of activity with citrate, retains activity with isocitrate Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.405
-
isocitrate 60┬░C, pH 8.0 Thermus thermophilus
7.486
-
homoisocitrate 60┬░C, pH 8.0 Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72IW9 expression in Escherichia coli
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72IW9 expression in Escherichia coli
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
homoisocitrate + NAD+
-
Thermus thermophilus ? + NADH
-
?
homoisocitrate + NAD+
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ? + NADH
-
?
isocitrate + NAD+ about 20times more efficient than homoisocitrate Thermus thermophilus 2-oxoglutarate + CO2 + NADH + H+
-
?
isocitrate + NAD+ about 20times more efficient than homoisocitrate Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 2-oxoglutarate + CO2 + NADH + H+
-
?
additional information no substrate: 3-isopropylmalate Thermus thermophilus ?
-
?
additional information no substrate: 3-isopropylmalate Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
?

Subunits

Subunits Comment Organism
tetramer sedimentation equilibrium analysis, SDS-PAGE Thermus thermophilus

Synonyms

Synonyms Comment Organism
HICDH
-
Thermus thermophilus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
90
-
half-life 16.7 h Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
171
-
isocitrate 60┬░C, pH 8.0 Thermus thermophilus
171
-
homoisocitrate 60┬░C, pH 8.0 Thermus thermophilus