BRENDA - Enzyme Database
show all sequences of 1.1.1.266

A novel NDP-6-deoxyhexosyl-4-ulose reductase in the pathway for the synthesis of thymidine diphosphate-D-fucose

Yoshida, Y.; Nakano, Y.; Nezu, T.; Yamashita, Y.; Koga, T.; J. Biol. Chem. 274, 16933-16939 (1999)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Aggregatibacter actinomycetemcomitans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.029
-
NADPH
sequential reaction mechanism
Aggregatibacter actinomycetemcomitans
0.097
-
dTDP-4-keto-6-deoxy-D-glucose
sequential reaction mechnism
Aggregatibacter actinomycetemcomitans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dTDP-D-fucose + NADP+
Aggregatibacter actinomycetemcomitans
-
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
Aggregatibacter actinomycetemcomitans
r
dTDP-D-fucose + NADP+
Aggregatibacter actinomycetemcomitans Y4
-
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
Aggregatibacter actinomycetemcomitans Y4
r
Organism
Organism
UniProt
Commentary
Textmining
Aggregatibacter actinomycetemcomitans
-
serotype b
-
Aggregatibacter actinomycetemcomitans Y4
-
serotype b
-
Purification (Commentary)
Purification (Commentary)
Organism
to homogeneity, recombinant enzyme
Aggregatibacter actinomycetemcomitans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
dTDP-D-fucose + NADP+
-
246376
Aggregatibacter actinomycetemcomitans
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
246376
Aggregatibacter actinomycetemcomitans
r
dTDP-D-fucose + NADP+
-
246376
Aggregatibacter actinomycetemcomitans Y4
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
246376
Aggregatibacter actinomycetemcomitans Y4
r
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
43.5
-
dTDP-4-keto-6-deoxy-D-glucose
-
Aggregatibacter actinomycetemcomitans
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Aggregatibacter actinomycetemcomitans
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Aggregatibacter actinomycetemcomitans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Aggregatibacter actinomycetemcomitans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.029
-
NADPH
sequential reaction mechanism
Aggregatibacter actinomycetemcomitans
0.097
-
dTDP-4-keto-6-deoxy-D-glucose
sequential reaction mechnism
Aggregatibacter actinomycetemcomitans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
dTDP-D-fucose + NADP+
Aggregatibacter actinomycetemcomitans
-
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
Aggregatibacter actinomycetemcomitans
r
dTDP-D-fucose + NADP+
Aggregatibacter actinomycetemcomitans Y4
-
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
Aggregatibacter actinomycetemcomitans Y4
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, recombinant enzyme
Aggregatibacter actinomycetemcomitans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
dTDP-D-fucose + NADP+
-
246376
Aggregatibacter actinomycetemcomitans
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
246376
Aggregatibacter actinomycetemcomitans
r
dTDP-D-fucose + NADP+
-
246376
Aggregatibacter actinomycetemcomitans Y4
dTDP-4-dehydro-6-deoxy-D-glucose + NADPH
-
246376
Aggregatibacter actinomycetemcomitans Y4
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
43.5
-
dTDP-4-keto-6-deoxy-D-glucose
-
Aggregatibacter actinomycetemcomitans
Other publictions for EC 1.1.1.266
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
673889
Zayni
The dTDP-4-dehydro-6-deoxygluc ...
Anoxybacillus tepidamans, Anoxybacillus tepidamans GS5-97T
Glycobiology
17
433-443
2007
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5
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1
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246376
Yoshida
A novel NDP-6-deoxyhexosyl-4-u ...
Aggregatibacter actinomycetemcomitans, Aggregatibacter actinomycetemcomitans Y4
J. Biol. Chem.
274
16933-16939
1999
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1
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2
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2
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1
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2
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1
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1
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1
1
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2
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2
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1
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2
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1
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