BRENDA - Enzyme Database show
show all sequences of 1.1.1.265

Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae

Van Iersel, M.F.; Eppink, M.H.; van Berkel, W.J.; Rombouts, F.M.; Abee, T.; Appl. Environ. Microbiol. 63, 4079-4082 (1997)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
KCl
5-6fold increasing activity for NADPH-dependent activity at 0.2 M
Saccharomyces cerevisiae
NaCl
5-6fold increasing activity for NADPH-dependent activity at 0.2 M
Saccharomyces cerevisiae
Application
Application
Commentary
Organism
nutrition
essential in removal of the worthy off-flavours in beer during fermentation
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-methylbutanal
presence of NADPH, Ki: 2.74 mM, presence of NADH, Ki: 4.61 mM
Saccharomyces cerevisiae
3-Methylbutanal
presence of NADPH, Ki: 7.14 mM, presence of NADH, Ki: 253 mM
Saccharomyces cerevisiae
Butanal
presence of NADPH, Ki: 16.6 mM
Saccharomyces cerevisiae
Heptanal
presence of NADPH, Ki: 0.88 mM, presence of NADH, Ki: 1.17 mM
Saccharomyces cerevisiae
Hexanal
presence of NADPH, Ki: 0.79 mM, presence of NADH, Ki: 2.8 mM
Saccharomyces cerevisiae
KCl
5-6fold decreasing activity at 0.2 M for NADH-dependent activity
Saccharomyces cerevisiae
NaCl
5-6fold decreasing activity at 0.2 M for NADH-dependent activity
Saccharomyces cerevisiae
NADP+
complete inhibition at a 6.7fold excess of NADP+ in a reduction assay with 0.15 mM NADH
Saccharomyces cerevisiae
pentanal
presence of NADPH, Ki: 3.9 mM, presence of NADH, Ki: 13.9 mM
Saccharomyces cerevisiae
propanal
presence of NADPH, Ki: 46.6 mM
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADPH
-
Saccharomyces cerevisiae
0.16
-
pentanal
presence of NADPH
Saccharomyces cerevisiae
0.18
-
Hexanal
presence of NADPH
Saccharomyces cerevisiae
0.21
-
3-Methylbutanal
presence of NADPH
Saccharomyces cerevisiae
0.27
-
Heptanal
presence of NADPH
Saccharomyces cerevisiae
0.83
-
Hexanal
presence of NADH
Saccharomyces cerevisiae
1.22
-
Hexanol
presence of NADP+
Saccharomyces cerevisiae
1.69
-
trans-2-hexenol
presence of NADP+
Saccharomyces cerevisiae
1.7
-
NADH
-
Saccharomyces cerevisiae
1.85
-
2-methylbutanal
presence of NADPH
Saccharomyces cerevisiae
1.89
-
3-Methylbutanal
presence of NADH
Saccharomyces cerevisiae
2.76
-
Butanal
presence of NADPH
Saccharomyces cerevisiae
3.01
-
pentanal
presence of NADH
Saccharomyces cerevisiae
4.25
-
Heptanal
presence of NADH
Saccharomyces cerevisiae
17.7
-
2-methylbutanal
presence of NADH
Saccharomyces cerevisiae
23.1
-
Butanal
presence of NADH
Saccharomyces cerevisiae
27
-
propanal
presence of NADH
Saccharomyces cerevisiae
38.9
-
propanal
presence of NADPH
Saccharomyces cerevisiae
158
-
acetaldehyde
presence of NADPH
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-methylbutanal + NAD(P)H
Saccharomyces cerevisiae
-
3-methylbutanol + NAD(P)+
-
Saccharomyces cerevisiae
r
acetaldehyde + NADPH + H+
Saccharomyces cerevisiae
-
ethanol + NADP+
-
Saccharomyces cerevisiae
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
brewer's yeast
-
Purification (Commentary)
Commentary
Organism
to homogeneity, chromatography techniques
Saccharomyces cerevisiae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Saccharomyces cerevisiae
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
61.6
-
in the presence of NADPH
Saccharomyces cerevisiae
93.9
-
in the presence of NADH
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-pentanedione + NAD(P)H
very low activity
286203
Saccharomyces cerevisiae
? + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
2-methylbutanal + NAD(P)H
-
286203
Saccharomyces cerevisiae
2-methylbutanol + NAD(P)+
-
286203
Saccharomyces cerevisiae
r
3-methylbutanal + NAD(P)H
-
286203
Saccharomyces cerevisiae
3-methylbutanol + NAD(P)+
-
286203
Saccharomyces cerevisiae
r
acetaldehyde + NADPH + H+
-
286203
Saccharomyces cerevisiae
ethanol + NADP+
-
286203
Saccharomyces cerevisiae
r
benzaldehyde + NAD(P)H
20-50% of the activity with 3-methylbutanal for aromatic aldehydes
286203
Saccharomyces cerevisiae
benzyl alcohol + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
butanal + NAD(P)H + H+
-
286203
Saccharomyces cerevisiae
butanol + NAD(P)+
-
286203
Saccharomyces cerevisiae
r
diacetyl + NAD(P)H
very low activity
286203
Saccharomyces cerevisiae
? + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
furaldehyde + NAD(P)H
20-50% of the activity with 3-methylbutanal for aromatic aldehydes
286203
Saccharomyces cerevisiae
furfuryl alcohol + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
heptanal + NADPH
preference for long and branched-chain substrates with up to seven carbon atoms
286203
Saccharomyces cerevisiae
heptanol + NADP+
-
286203
Saccharomyces cerevisiae
r
hexanal + NADPH + H+
-
286203
Saccharomyces cerevisiae
hexanol + NADP+
-
286203
Saccharomyces cerevisiae
r
p-anisaldehyde + NAD(P)H
20-50% of the activity with 3-methylbutanal for aromatic aldehydes
286203
Saccharomyces cerevisiae
p-anisalcohol + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
pentanal + NADPH
-
286203
Saccharomyces cerevisiae
pentanol + NADP+
-
286203
Saccharomyces cerevisiae
r
propanal + NAD(P)H + H+
-
286203
Saccharomyces cerevisiae
propanol + NAD(P)+
-
286203
Saccharomyces cerevisiae
r
trans-2-hexenol + NADP+
-
286203
Saccharomyces cerevisiae
trans-2-hexenal + NADPH
-
286203
Saccharomyces cerevisiae
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 37000, SDS-PAGE
Saccharomyces cerevisiae
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.55
-
Hexanol
in the presence of NADP+
Saccharomyces cerevisiae
5.6
-
acetaldehyde
in the presence of NADPH
Saccharomyces cerevisiae
14
-
trans-2-hexenol
in the presence of NADP+
Saccharomyces cerevisiae
47.3
-
Butanal
presence of NADH
Saccharomyces cerevisiae
50.1
-
Hexanal
presence of NADH
Saccharomyces cerevisiae
55
-
propanal
presence of NADPH
Saccharomyces cerevisiae
56.6
-
pentanal
presence of NADPH
Saccharomyces cerevisiae
57
-
Butanal
presence of NADPH
Saccharomyces cerevisiae
71.1
-
Hexanal
presence of NADPH
Saccharomyces cerevisiae
72.9
-
Heptanal
presence of NADPH
Saccharomyces cerevisiae
74.9
-
3-Methylbutanal
presence of NADPH
Saccharomyces cerevisiae
81.6
-
pentanal
presence of NADH
Saccharomyces cerevisiae
84
-
propanal
presence of NADH
Saccharomyces cerevisiae
91.8
-
3-Methylbutanal
presence of NADH
Saccharomyces cerevisiae
92.3
-
2-methylbutanal
presence of NADH
Saccharomyces cerevisiae
101
-
Heptanal
presence of NADH
Saccharomyces cerevisiae
113
-
2-methylbutanal
presence of NADPH
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
NADH-dependent activity, highest activity at low ionic strength
Saccharomyces cerevisiae
8.5
-
NADPH-dependent activity, highest activity at high ionic strength
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Saccharomyces cerevisiae
NADPH
preferred cofactor
Saccharomyces cerevisiae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
KCl
5-6fold increasing activity for NADPH-dependent activity at 0.2 M
Saccharomyces cerevisiae
NaCl
5-6fold increasing activity for NADPH-dependent activity at 0.2 M
Saccharomyces cerevisiae
Application (protein specific)
Application
Commentary
Organism
nutrition
essential in removal of the worthy off-flavours in beer during fermentation
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Saccharomyces cerevisiae
NADPH
preferred cofactor
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-methylbutanal
presence of NADPH, Ki: 2.74 mM, presence of NADH, Ki: 4.61 mM
Saccharomyces cerevisiae
3-Methylbutanal
presence of NADPH, Ki: 7.14 mM, presence of NADH, Ki: 253 mM
Saccharomyces cerevisiae
Butanal
presence of NADPH, Ki: 16.6 mM
Saccharomyces cerevisiae
Heptanal
presence of NADPH, Ki: 0.88 mM, presence of NADH, Ki: 1.17 mM
Saccharomyces cerevisiae
Hexanal
presence of NADPH, Ki: 0.79 mM, presence of NADH, Ki: 2.8 mM
Saccharomyces cerevisiae
KCl
5-6fold decreasing activity at 0.2 M for NADH-dependent activity
Saccharomyces cerevisiae
NaCl
5-6fold decreasing activity at 0.2 M for NADH-dependent activity
Saccharomyces cerevisiae
NADP+
complete inhibition at a 6.7fold excess of NADP+ in a reduction assay with 0.15 mM NADH
Saccharomyces cerevisiae
pentanal
presence of NADPH, Ki: 3.9 mM, presence of NADH, Ki: 13.9 mM
Saccharomyces cerevisiae
propanal
presence of NADPH, Ki: 46.6 mM
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADPH
-
Saccharomyces cerevisiae
0.16
-
pentanal
presence of NADPH
Saccharomyces cerevisiae
0.18
-
Hexanal
presence of NADPH
Saccharomyces cerevisiae
0.21
-
3-Methylbutanal
presence of NADPH
Saccharomyces cerevisiae
0.27
-
Heptanal
presence of NADPH
Saccharomyces cerevisiae
0.83
-
Hexanal
presence of NADH
Saccharomyces cerevisiae
1.22
-
Hexanol
presence of NADP+
Saccharomyces cerevisiae
1.69
-
trans-2-hexenol
presence of NADP+
Saccharomyces cerevisiae
1.7
-
NADH
-
Saccharomyces cerevisiae
1.85
-
2-methylbutanal
presence of NADPH
Saccharomyces cerevisiae
1.89
-
3-Methylbutanal
presence of NADH
Saccharomyces cerevisiae
2.76
-
Butanal
presence of NADPH
Saccharomyces cerevisiae
3.01
-
pentanal
presence of NADH
Saccharomyces cerevisiae
4.25
-
Heptanal
presence of NADH
Saccharomyces cerevisiae
17.7
-
2-methylbutanal
presence of NADH
Saccharomyces cerevisiae
23.1
-
Butanal
presence of NADH
Saccharomyces cerevisiae
27
-
propanal
presence of NADH
Saccharomyces cerevisiae
38.9
-
propanal
presence of NADPH
Saccharomyces cerevisiae
158
-
acetaldehyde
presence of NADPH
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-methylbutanal + NAD(P)H
Saccharomyces cerevisiae
-
3-methylbutanol + NAD(P)+
-
Saccharomyces cerevisiae
r
acetaldehyde + NADPH + H+
Saccharomyces cerevisiae
-
ethanol + NADP+
-
Saccharomyces cerevisiae
r
Purification (Commentary) (protein specific)
Commentary
Organism
to homogeneity, chromatography techniques
Saccharomyces cerevisiae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Saccharomyces cerevisiae
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
61.6
-
in the presence of NADPH
Saccharomyces cerevisiae
93.9
-
in the presence of NADH
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-pentanedione + NAD(P)H
very low activity
286203
Saccharomyces cerevisiae
? + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
2-methylbutanal + NAD(P)H
-
286203
Saccharomyces cerevisiae
2-methylbutanol + NAD(P)+
-
286203
Saccharomyces cerevisiae
r
3-methylbutanal + NAD(P)H
-
286203
Saccharomyces cerevisiae
3-methylbutanol + NAD(P)+
-
286203
Saccharomyces cerevisiae
r
acetaldehyde + NADPH + H+
-
286203
Saccharomyces cerevisiae
ethanol + NADP+
-
286203
Saccharomyces cerevisiae
r
benzaldehyde + NAD(P)H
20-50% of the activity with 3-methylbutanal for aromatic aldehydes
286203
Saccharomyces cerevisiae
benzyl alcohol + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
butanal + NAD(P)H + H+
-
286203
Saccharomyces cerevisiae
butanol + NAD(P)+
-
286203
Saccharomyces cerevisiae
r
diacetyl + NAD(P)H
very low activity
286203
Saccharomyces cerevisiae
? + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
furaldehyde + NAD(P)H
20-50% of the activity with 3-methylbutanal for aromatic aldehydes
286203
Saccharomyces cerevisiae
furfuryl alcohol + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
heptanal + NADPH
preference for long and branched-chain substrates with up to seven carbon atoms
286203
Saccharomyces cerevisiae
heptanol + NADP+
-
286203
Saccharomyces cerevisiae
r
hexanal + NADPH + H+
-
286203
Saccharomyces cerevisiae
hexanol + NADP+
-
286203
Saccharomyces cerevisiae
r
p-anisaldehyde + NAD(P)H
20-50% of the activity with 3-methylbutanal for aromatic aldehydes
286203
Saccharomyces cerevisiae
p-anisalcohol + NAD(P)+
-
286203
Saccharomyces cerevisiae
?
pentanal + NADPH
-
286203
Saccharomyces cerevisiae
pentanol + NADP+
-
286203
Saccharomyces cerevisiae
r
propanal + NAD(P)H + H+
-
286203
Saccharomyces cerevisiae
propanol + NAD(P)+
-
286203
Saccharomyces cerevisiae
r
trans-2-hexenol + NADP+
-
286203
Saccharomyces cerevisiae
trans-2-hexenal + NADPH
-
286203
Saccharomyces cerevisiae
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 37000, SDS-PAGE
Saccharomyces cerevisiae
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.55
-
Hexanol
in the presence of NADP+
Saccharomyces cerevisiae
5.6
-
acetaldehyde
in the presence of NADPH
Saccharomyces cerevisiae
14
-
trans-2-hexenol
in the presence of NADP+
Saccharomyces cerevisiae
47.3
-
Butanal
presence of NADH
Saccharomyces cerevisiae
50.1
-
Hexanal
presence of NADH
Saccharomyces cerevisiae
55
-
propanal
presence of NADPH
Saccharomyces cerevisiae
56.6
-
pentanal
presence of NADPH
Saccharomyces cerevisiae
57
-
Butanal
presence of NADPH
Saccharomyces cerevisiae
71.1
-
Hexanal
presence of NADPH
Saccharomyces cerevisiae
72.9
-
Heptanal
presence of NADPH
Saccharomyces cerevisiae
74.9
-
3-Methylbutanal
presence of NADPH
Saccharomyces cerevisiae
81.6
-
pentanal
presence of NADH
Saccharomyces cerevisiae
84
-
propanal
presence of NADH
Saccharomyces cerevisiae
91.8
-
3-Methylbutanal
presence of NADH
Saccharomyces cerevisiae
92.3
-
2-methylbutanal
presence of NADH
Saccharomyces cerevisiae
101
-
Heptanal
presence of NADH
Saccharomyces cerevisiae
113
-
2-methylbutanal
presence of NADPH
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
7
NADH-dependent activity, highest activity at low ionic strength
Saccharomyces cerevisiae
8.5
-
NADPH-dependent activity, highest activity at high ionic strength
Saccharomyces cerevisiae
Other publictions for EC 1.1.1.265
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737753
Guo
Structural insights into the c ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1844
1486-1492
2014
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1
1
11
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8
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1
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1
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8
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1
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11
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8
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1
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8
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8
8
710768
Breicha
Crystallization and preliminar ...
Saccharomyces cerevisiae
Acta Crystallogr. Sect. F
66
838-841
2010
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1
1
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2
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686820
Hauser
A transcriptome analysis of is ...
Saccharomyces cerevisiae
FEMS Yeast Res.
7
84-92
2007
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1
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-
1
-
2
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2
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2
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2
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1
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1
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2
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739760
Warringer
Involvement of yeast YOL151W/G ...
Saccharomyces cerevisiae
Yeast
23
389-398
2006
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-
-
-
-
-
-
-
-
-
-
-
-
2
-
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1
1
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246373
Perpete
Contribution of 3-methylthiopr ...
Candida boidinii, Saccharomyces bayanus, Saccharomyces cerevisiae, Saccharomyces ludwigii
J. Agric. Food Chem.
47
2374-2378
1999
-
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-
1
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4
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6
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4
-
-
5
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8
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8
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1
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4
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4
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5
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246375
Van Nedervelde
-
Purificationa nd characterizat ...
Saccharomyces cerevisiae
Proc. Congr. Eur. Brew. Conv.
26
447-454
1997
-
-
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10
-
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4
-
1
-
-
1
-
-
1
1
-
12
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
10
-
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4
-
-
-
1
-
1
1
-
12
-
-
-
-
-
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286203
Van Iersel
Purification and characterizat ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
63
4079-4082
1997
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