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Literature summary for 1.1.1.237 extracted from

  • Fujii, T.; Shimizu, M.; Doi, Y.; Fujita, T.; Ito, T.; Miura, D.; Wariishi, H.; Takaya, N.
    Novel fungal phenylpyruvate reductase belongs to D-isomer-specific 2-hydroxyacid dehydrogenase family (2011), Biochim. Biophys. Acta, 1814, 1669-1676.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Wickerhamia fluorescens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, less than 10% residual activity Wickerhamia fluorescens
Fe2+ 1 mM, less than 10% residual activity Wickerhamia fluorescens
Hg2+ 1 mM, less than 10% residual activity Wickerhamia fluorescens
WO42- 1 mM, less than 10% residual activity Wickerhamia fluorescens
Zn2+ 1 mM, less than 10% residual activity Wickerhamia fluorescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADPH pH 6.5, 25┬░C Wickerhamia fluorescens
0.1
-
NADH pH 6.5, 25┬░C Wickerhamia fluorescens
0.4
-
phenylpyruvate pH 6.5, 25┬░C Wickerhamia fluorescens
0.64
-
hydroxyphenylpyruvate pH 6.5, 25┬░C Wickerhamia fluorescens
3.5
-
Hydroxypyruvate pH 6.5, 25┬░C Wickerhamia fluorescens
18.9
-
glyoxylate pH 6.5, 25┬░C Wickerhamia fluorescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, SDS-PAGE, 2 * 40300, calculated Wickerhamia fluorescens
40300
-
2 * 40000, SDS-PAGE, 2 * 40300, calculated Wickerhamia fluorescens
75000
-
gel filtration Wickerhamia fluorescens

Organism

Organism UniProt Comment Textmining
Wickerhamia fluorescens F1T2J9
-
-
Wickerhamia fluorescens TK1 F1T2J9
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
727
-
pH 6.5, 25┬░C Wickerhamia fluorescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + NADPH + H+
-
Wickerhamia fluorescens D-(4-hydroxyphenyl)lactate + NADP+
-
?
4-hydroxyphenylpyruvate + NADPH + H+
-
Wickerhamia fluorescens TK1 D-(4-hydroxyphenyl)lactate + NADP+
-
?
glyoxylate + NADPH + H+
-
Wickerhamia fluorescens glycolate + NADP+
-
?
glyoxylate + NADPH + H+
-
Wickerhamia fluorescens TK1 glycolate + NADP+
-
?
hydroxypyruvate + NADH + H+
-
Wickerhamia fluorescens D-glycerate + NAD+
-
?
hydroxypyruvate + NADH + H+
-
Wickerhamia fluorescens TK1 D-glycerate + NAD+
-
?
additional information no substrates: pyruvate, oxaloacetate or benzoylformate Wickerhamia fluorescens ?
-
?
additional information no substrates: pyruvate, oxaloacetate or benzoylformate Wickerhamia fluorescens TK1 ?
-
?
phenylpyruvate + NADPH + H+ more than 99.9% D-isomer, L-isomer below limits of detection Wickerhamia fluorescens D-phenyllactate + NADP+
-
?
phenylpyruvate + NADPH + H+ more than 99.9% D-isomer, L-isomer below limits of detection Wickerhamia fluorescens TK1 D-phenyllactate + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE, 2 * 40300, calculated Wickerhamia fluorescens

Synonyms

Synonyms Comment Organism
Ppr
-
Wickerhamia fluorescens
pprA
-
Wickerhamia fluorescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.1
-
Hydroxypyruvate pH 6.5, 25┬░C Wickerhamia fluorescens
19
-
glyoxylate pH 6.5, 25┬░C Wickerhamia fluorescens
31
-
NADH pH 6.5, 25┬░C Wickerhamia fluorescens
73
-
hydroxyphenylpyruvate pH 6.5, 25┬░C Wickerhamia fluorescens
121
-
NADPH pH 6.5, 25┬░C Wickerhamia fluorescens
150
-
phenylpyruvate pH 6.5, 25┬░C Wickerhamia fluorescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Wickerhamia fluorescens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Wickerhamia fluorescens
NADH NADPH is preferred over NADH Wickerhamia fluorescens
NADP+
-
Wickerhamia fluorescens
NADPH preferred over NADH Wickerhamia fluorescens

Expression

Organism Comment Expression
Wickerhamia fluorescens presence of phenylalanine results in up to 40fold increase in transcripts up

General Information

General Information Comment Organism
physiological function Wickerhamia fluorescens efficiently converts phenylalanine and phenylpyruvate to D-phenyllactate. These compounds up-regulate the transcription of enzyme gene pprA Wickerhamia fluorescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
glyoxylate pH 6.5, 25┬░C Wickerhamia fluorescens
2.6
-
Hydroxypyruvate pH 6.5, 25┬░C Wickerhamia fluorescens
110
-
hydroxyphenylpyruvate pH 6.5, 25┬░C Wickerhamia fluorescens
310
-
NADH pH 6.5, 25┬░C Wickerhamia fluorescens
380
-
phenylpyruvate pH 6.5, 25┬░C Wickerhamia fluorescens
12000
-
NADPH pH 6.5, 25┬░C Wickerhamia fluorescens