Cloned (Comment) | Organism |
---|---|
expression in COS-7 cells | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
H170C | fully active | Bos taurus |
K225S | 2fold as active as wild-type | Bos taurus |
N306Y | half as active as wild-type | Bos taurus |
R223L | 5fold as active as wild-type | Bos taurus |
R27E | fully active | Bos taurus |
R91Q | fully active | Bos taurus |
S301R | 2.5fold as active as wild-type | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
9,10-phenanthrenequinone | native lung prostaglandin F synthase | Bos taurus | |
0.0011 | - |
9,10-phenanthrenequinone | expressed liver prostaglandin F synthase | Bos taurus | |
0.002 | - |
9,10-phenanthrenequinone | native liver prostaglandin F synthase | Bos taurus | |
0.01 | - |
prostaglandin H2 | native lung prostaglandin F synthase | Bos taurus | |
0.01 | - |
Prostaglandin D2 | native liver prostaglandin F synthase | Bos taurus | |
0.015 | - |
Prostaglandin D2 | expressed liver prostaglandin F synthase | Bos taurus | |
0.025 | - |
prostaglandin H2 | native and expressed liver prostaglandin F synthase | Bos taurus | |
0.12 | - |
Prostaglandin D2 | native lung prostaglandin F synthase | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36740 | - |
amino acid analysis, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
two isozymes, lung-type and liver-type | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
lung | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH | - |
Bos taurus | (5Z,13E)-(15S)-9alpha,11beta,15-trihydroxyprosta-5,13-dienoate + NADP+ | - |
? | |
9,10-phenanthrenequinone + NADPH | - |
Bos taurus | ? | - |
? | |
prostaglandin H2 + NADPH + H+ | - |
Bos taurus | (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bos taurus |