Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
rutin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | recombinant enzyme, steady-state kinetics, molecular modeling and substrate docking studies | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
daunorubicin + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | daunorubicinol + NADP+ | - |
? | |
doxorubicin + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | doxorubicinol + NADP+ | - |
? | |
menadione + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | ? | - |
? | |
mitroxantrone + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | ? | - |
? | |
naphthazarin + NADPH + H+ | Homo sapiens | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16152 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ | molecular modeling and enzyme-substrate docking studies, reaction mechanism with anthracyclin substrates | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
13-deoxydoxorubicin + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | ? | - |
? | |
daunorubicin + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | daunorubicinol + NADP+ | - |
? | |
daunorubicin + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | daunorubicinol + NADP+ | - |
? | |
doxorubicin + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | doxorubicinol + NADP+ | - |
? | |
doxorubicin + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | doxorubicinol + NADP+ | - |
? | |
menadione + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | ? | - |
? | |
menadione + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | ? | - |
? | |
mitroxantrone + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | ? | - |
? | |
mitroxantrone + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | ? | - |
? | |
naphthazarin + NADPH + H+ | reduction of the anthracycline C13 carbonyl results in a 13-hydroxy metabolite that elicits potent cardiotoxic effects while possessing significantly reduced anticancer properties | Homo sapiens | ? | - |
? | |
naphthazarin + NADPH + H+ | an anthracyclin, formation of a 13-hydroxy-anthracyclin | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | molecular modeling and generation of a three-dimensional structural model | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
carbonyl reductase 1 | - |
Homo sapiens |
CBR1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Homo sapiens |