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Literature summary for extracted from

  • Baker, P.J.; Britton, K.L.; Fisher, M.; Esclapez, J.; Pire, C.; Bonete, M.J.; Ferrer, J.; Rice, D.W.
    Active site dynamics in the zinc-dependent medium chain alcohol dehydrogenase superfamily (2009), Proc. Natl. Acad. Sci. USA, 106, 779-784.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
Haloferax mediterranei
the structures of GlcDH in binary complexes with either NADP+/Zn2+ or NADPH/Zn2+, a ternary complex with NADP+/Zn2+/glucose, and a nonproductive ternary complex with NADP+/Zn2+ /gluconolactone are determined from isomorphous crystals, in the presence of zinc (1 mM) and either 5 mM NADP+, by using 1.4-1.6 M sodium citrate as the precipitant (in 100 mM HEPES, pH 7.0, and 2 M NaCl) or 5 mM NADPH, using 67-72% (w/v) 2-methyl-2,4-pentanediol as precipitant (in 100 mM HEPES, pH 7.5, and 2 M NaCl) Haloferax mediterranei


Metals/Ions Comment Organism Structure
Zn2+ dependent on Haloferax mediterranei
Zn2+ zinc is an important component of the catalytic centre Haloferax mediterranei


Organism UniProt Comment Textmining
Haloferax mediterranei Q977U7
Haloferax mediterranei Q977U7 extremely halophilic archaeon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + NADP+
Haloferax mediterranei D-glucono-1,5-lactone + NADPH + H+


Synonyms Comment Organism
Haloferax mediterranei
glucose dehydrogenase
Haloferax mediterranei


Cofactor Comment Organism Structure
Haloferax mediterranei