Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
apo form, in complex with NAD+ and glycerol, or with only NAD+, sitting drop vapor diffusion method, using 0.1 M HEPES (pH 7.0), 10% (w/v) PEG 4000, 10% (v/v) 2-propanol, or 0.2 M NaCl, 0.1 M HEPES (pH 7.5), 25% (w/v) PEG3350 | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
M333A | inactive | Mus musculus |
M333E | the mutation affects the substrate's affinity for the enzyme | Mus musculus |
R180K | the mutation has little effect on NAD+ binding affinity, whereas affects the substrate's affinity for the enzyme | Mus musculus |
S133A | inactive | Mus musculus |
T237A | inactive | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.67 | - |
NAD+ | wild type enzyme, at pH 7.5 and 37°C | Mus musculus | |
2.06 | - |
NAD+ | mutant enzyme R180K, at pH 7.5 and 37°C | Mus musculus | |
72.4 | - |
L-threonine | wild type enzyme, at pH 7.5 and 37°C | Mus musculus | |
151.9 | - |
L-threonine | mutant enzyme M333E, at pH 7.5 and 37°C | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Mus musculus | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8K3F7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-chelating column chromatography and Superdex 200 gel filtration | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Mus musculus | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-threonine dehydrogenase | - |
Mus musculus |
TDH | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
46.7 | - |
L-threonine | mutant enzyme M333E, at pH 7.5 and 37°C | Mus musculus | |
47.3 | - |
L-threonine | wild type enzyme, at pH 7.5 and 37°C | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
L-threonine | mutant enzyme R180K, at pH 7.5 and 37°C | Mus musculus | |
0.004 | - |
L-threonine | mutant enzyme S133A, at pH 7.5 and 37°C | Mus musculus | |
0.004 | - |
L-threonine | mutant enzyme T237A, at pH 7.5 and 37°C | Mus musculus | |
0.006 | - |
L-threonine | mutant enzyme M333A, at pH 7.5 and 37°C | Mus musculus | |
0.3 | - |
L-threonine | mutant enzyme M333E, at pH 7.5 and 37°C | Mus musculus | |
0.7 | - |
L-threonine | wild type enzyme, at pH 7.5 and 37°C | Mus musculus |