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beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
fructofuranosyl nystose
alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride + 1-kestose
fructofuranosyl nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
nystose
alpha-D-fructofuranose beta-D-fructofuranose1,2':2,1'-dianhydride + sucrose
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
[inulin]n
alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride + [inulin]n-2
[inulin]n
alpha-D-fructofuranose beta-D-fructofuranose1,2':2,1'-dianhydride + [inulin]n-2
additional information
?
-
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside

di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
E9URP8
Substrates: -
Products: -
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
Substrates: -
Products: -
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
A1SCS9
Substrates: -
Products: -
?
beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-beta-D-fructofuranosyl-(2->1)-alpha-D-glucopyranoside
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
A1SCS9
Substrates: -
Products: -
?
fructofuranosyl nystose

alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride + 1-kestose
-
Substrates: -
Products: -
?
fructofuranosyl nystose
alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride + 1-kestose
-
Substrates: -
Products: -
?
fructofuranosyl nystose

di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
Substrates: -
Products: -
?
fructofuranosyl nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose
-
Substrates: -
Products: -
?
inulin

3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: i.e. DFA I
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: -
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: -
?
inulin
3 di-D-fructofuranose-1,2':2,1'-dianhydride + ?
-
Substrates: -
Products: -
?
inulin

?
-
Substrates: -
Products: -
?
inulin
?
-
Substrates: -
Products: -
?
inulin

di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
-
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + 1-kestose + nystose + fructofuranosyl nystose
-
Substrates: -
Products: -
?
inulin

di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
E9URP8
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
A1SCS9
Substrates: -
Products: -
?
inulin
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose + 1-kestose
A1SCS9
Substrates: -
Products: -
?
nystose

alpha-D-fructofuranose beta-D-fructofuranose1,2':2,1'-dianhydride + sucrose
-
Substrates: -
Products: -
?
nystose
alpha-D-fructofuranose beta-D-fructofuranose1,2':2,1'-dianhydride + sucrose
-
Substrates: -
Products: -
?
nystose

di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
E9URP8
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
A1SCS9
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
A1SCS9
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
Substrates: -
Products: -
?
nystose
di-D-fructofuranose-1,2':2,1'-dianhydride + sucrose
-
Substrates: -
Products: -
?
[inulin]n

alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride + [inulin]n-2
-
Substrates: -
Products: -
?
[inulin]n
alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride + [inulin]n-2
-
Substrates: -
Products: -
?
[inulin]n

alpha-D-fructofuranose beta-D-fructofuranose1,2':2,1'-dianhydride + [inulin]n-2
-
Substrates: -
Products: -
?
[inulin]n
alpha-D-fructofuranose beta-D-fructofuranose1,2':2,1'-dianhydride + [inulin]n-2
-
Substrates: -
Products: -
?
additional information

?
-
-
Substrates: no substrate: 1-kestose
Products: -
?
additional information
?
-
-
Substrates: no substrate: 1-kestose
Products: -
?
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A257S
mutation of residue in central tunnel, no significant change of temperature stability
A257S/G281S G281S
mutation of residues in central tunnel, increase in temperature stability by 3 degrees
A257T
mutation of residue in central tunnel, 2.8 degrees decrease of temperature stability
D183A
-
complete loss of activity
D183N
-
complete loss of activity
E194A
-
complete loss of activity
E194N
-
complete loss of activity
G121A/T122L
148.7% of wild-type activity, about 4.5 degrees increase in temperature stability
G236S/G281S/A257S/T313S/A314S
140.5% of wild-type activity, increase in temperature stability by 3.5 degrees
G281S
mutation of residue in central tunnel, 76% of wild-type activity, increase in temperature stability by 3 degrees
G281T
mutation of residue in central tunnel, no significant change of temperature stability
I80A
11.6% of wild-type activity, about 2 degrees increase in temperature stability
I80E
15.7% of wild-type activity, about 1.5 degrees increase in temperature stability
I80S
41.4% of wild-type activity, about 1 degrees increase in temperature stability
D183A
-
complete loss of activity
-
D183N
-
complete loss of activity
-
E194A
-
complete loss of activity
-
E194N
-
complete loss of activity
-
D184A
-
the mutant shows 18.51% of wild type activity
D184N
-
the mutant shows 14% of wild type activity
D291N
-
the mutant shows 13.18% of wild type activity
E195A
-
the mutant shows 16.14% of wild type activity
E195Q
-
the mutant shows 18.83% of wild type activity
E201I/Q234L/K310G
-
the mutant is able to retain native enzymatic activity and displays excellent thermal stability at 70°C compared to the wild type enzyme
E85A
-
the mutant shows 37.88% of wild type activity
Q69L/Q234L/K310G
-
the mutant is able to retain native enzymatic activity and displays excellent thermal stability at 70°C compared to the wild type enzyme
D184A
-
the mutant shows 18.51% of wild type activity
-
D184N
-
the mutant shows 14% of wild type activity
-
E195A
-
the mutant shows 16.14% of wild type activity
-
E195Q
-
the mutant shows 18.83% of wild type activity
-
E201I/Q234L/K310G
-
the mutant is able to retain native enzymatic activity and displays excellent thermal stability at 70°C compared to the wild type enzyme
-
E85A
-
the mutant shows 37.88% of wild type activity
-
Q69L/Q234L/K310G
-
the mutant is able to retain native enzymatic activity and displays excellent thermal stability at 70°C compared to the wild type enzyme
-
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Haraguchi, K.; Hayashi, K.; Yanagimoto, M.
Cloning of the gene of DFA I oligosaccharide-producing enzyme
Jpn. Agric. Res. Q
32
1-5
1998
Arthrobacter globiformis, Arthrobacter globiformis S14-3
-
brenda
Haraguchi, K.; Seki, K.; Kishimoto, M.; Nagata, T.; Kasumi, T.; Kainuma, K.; Kobayashi, S.
Cloning and nucleotide sequence of the inulin fructotransferase (DFA I-producing) gene of Arthrobacter globiformis S14-3
Biosci. Biotechnol. Biochem.
59
1809-1812
1995
Arthrobacter globiformis, Arthrobacter globiformis S14-3
brenda
Ueda, M.; Sashida, R.; Morimoto, Y.; Ohkishi, H.
Purification of inulin fructotransferase (DFA I-producing) from Arthrobacter sp. MCI2493 and production of DFA I from inulin by the enzyme
Biosci. Biotechnol. Biochem.
58
574-575
1994
Arthrobacter, Arthrobacter MC12493
brenda
Seki, K.; Haraguchi, K.; Kishimoto, M.; Koboyashi, S.; Kainuma, K.
Purification and properties of a novel inulin fructotransferase (DFA I-producing) from Arthrobacter globiformis S14-3
Agric. Biol. Chem.
53
2089-2094
1989
Arthrobacter globiformis, Arthrobacter globiformis S14-3
-
brenda
Haraguchi, K.; Yoshida, M.; Ohtsubo, K.i.
Purification and properties of a heat-stable inulin fructotransferase from Arthrobacter ureafaciens
Biotechnol. Lett.
25
1049-1053
2003
Paenarthrobacter ureafaciens, Paenarthrobacter ureafaciens A51-1
brenda
Haraguchi, K.; Yoshida, M.; Yamanaka, T.; Ohtsubo, K.i.
Purification and characterization of a heat stable inulin fructotransferase (DFA I-producing) from Arthrobacter pascens a62-1
Carbohydr. Polym.
53
501-505
2003
Paenarthrobacter ureafaciens, Paenarthrobacter ureafaciens a62-1
-
brenda
Zhu, Y.; Yu, S.; Huang, D.; Zhang, T.; Jiang, B.; Mu, W.
Identification of a novel DFA I-producing inulin fructotransferase from Streptomyces davawensis
Int. J. Biol. Macromol.
92
723-730
2016
Streptomyces davaonensis, Streptomyces davaonensis SK39.001
brenda
Yu, S.; Zhang, Y.; Zhu, Y.; Zhang, T.; Jiang, B.; Mu, W.
Improving the catalytic behavior of DFA I-forming inulin fructotransferase from Streptomyces davawensis with site-directed mutagenesis
J. Agric. Food Chem.
65
7579-7587
2017
Streptomyces davaonensis (K4QSF6)
brenda
Cheng, Y.; Yu, S.; Zhu, Y.; Zhang, T.; Jiang, B.; Mu, W.
Formation of di-D-fructofuranose-1,2' 2,1'-dianhydride by three novel inulin fructotransferases from the Nocardiaceae family
Process Biochem.
62
106-113
2017
Nocardioidaceae bacterium Broad-1 (E9URP8), Nocardioides luteus (A0A1J4N453), Nocardioides sp. (A1SCS9), Nocardioides sp. ATCC BAA-499 (A1SCS9)
-
brenda
Cheng, M.; Zhu, Y.; Chen, Z.; Guang, C.; Zhang, W.; Mu, W.
Biochemical characterization of a novel thermostable DFA I-forming inulin fructotransferases from Streptomyces peucetius subsp. caesius ATCC 27952
Enzyme Microb. Technol.
137
109519
2020
Streptomyces peucetius subsp. caesius, Streptomyces peucetius subsp. caesius ATCC 27952
brenda
Cheng, M.; Huang, Z.; Zhang, W.; Kim, B.; Mu, W.
Thermostability engineering of an inulin fructotransferase for the biosynthesis of difructose anhydride I
Enzyme Microb. Technol.
160
110097
2022
Streptomyces peucetius subsp. caesius, Streptomyces peucetius subsp. caesius ATCC 27952
brenda