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0.012 - 0.757
(4R)-4-hydroxy-2-oxopentanoate
0.013 - 0.228
(4S)-4-hydroxy-2-oxopentanoate
0.35
4-hydroxy-2-oxoheptanoate
pH 8.0, 25°C
0.037 - 1.1
4-hydroxy-2-oxohexanoate
0.041 - 0.94
4-hydroxy-2-oxopentanoate
124.6
glycolaldehyde
-
app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
16.8 - 136
propionaldehyde
additional information
additional information
-
0.012
(4R)-4-hydroxy-2-oxopentanoate
-
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.013
(4R)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.013
(4R)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.435
(4R)-4-hydroxy-2-oxopentanoate
-
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.757
(4R)-4-hydroxy-2-oxopentanoate
-
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.013
(4S)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.013
(4S)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.013
(4S)-4-hydroxy-2-oxopentanoate
-
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.089
(4S)-4-hydroxy-2-oxopentanoate
-
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.089
(4S)-4-hydroxy-2-oxopentanoate
-
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.222
(4S)-4-hydroxy-2-oxopentanoate
-
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.228
(4S)-4-hydroxy-2-oxopentanoate
-
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.037
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
0.117
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
0.117
4-hydroxy-2-oxohexanoate
-
wild-type protein, steady state, pH 8.0, 25°C
0.18
4-hydroxy-2-oxohexanoate
pH 8.0, 25°C
0.199
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
0.21
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
0.218
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
0.3
4-hydroxy-2-oxohexanoate
-
G322F mutant, steady state, pH 8.0, 25°C
0.35
4-hydroxy-2-oxohexanoate
-
G322L mutant, steady state, pH 8.0, 25°C
0.36
4-hydroxy-2-oxohexanoate
-
G323A mutant, steady state, pH 8.0, 25°C
0.37
4-hydroxy-2-oxohexanoate
-
G323L mutant, steady state, pH 8.0, 25°C
0.52
4-hydroxy-2-oxohexanoate
-
G322A mutant, steady state, pH 8.0, 25°C
1.1
4-hydroxy-2-oxohexanoate
-
G323F mutant, steady state, pH 8.0, 25°C
0.041
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
0.045
4-hydroxy-2-oxopentanoate
app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C
0.053
4-hydroxy-2-oxopentanoate
-
G322F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.055
4-hydroxy-2-oxopentanoate
app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C
0.06
4-hydroxy-2-oxopentanoate
-
G323A mutant, steady state, pH 8.0, 25°C
0.074
4-hydroxy-2-oxopentanoate
app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C
0.08
4-hydroxy-2-oxopentanoate
app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C
0.081
4-hydroxy-2-oxopentanoate
-
G322L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.089
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
0.089
4-hydroxy-2-oxopentanoate
-
wild-type protein, steady state, pH 8.0, 25°C
0.09
4-hydroxy-2-oxopentanoate
-
G322F mutant, steady state, pH 8.0, 25°C
0.095
4-hydroxy-2-oxopentanoate
app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C
0.13
4-hydroxy-2-oxopentanoate
-
G323F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.14
4-hydroxy-2-oxopentanoate
-
G322L mutant, steady state, pH 8.0, 25°C
0.158
4-hydroxy-2-oxopentanoate
-
wild-type protein, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.16
4-hydroxy-2-oxopentanoate
-
G323L mutant, steady state, pH 8.0, 25°C
0.18
4-hydroxy-2-oxopentanoate
-
G322A mutant, steady state, pH 8.0, 25°C
0.206
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
0.216
4-hydroxy-2-oxopentanoate
-
G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.22
4-hydroxy-2-oxopentanoate
pH 8.0, 25°C
0.257
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
0.295
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
0.94
4-hydroxy-2-oxopentanoate
-
G323F mutant, steady state, pH 8.0, 25°C
64.2
acetaldehyde
-
wild-type BphI, app. Km-value, pH 8.0, 25°C
64.2
acetaldehyde
-
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
64.3
acetaldehyde
-
app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
67
acetaldehyde
-
steady-state kinetic parameter, pH 8.0 and 25°C
70.7
acetaldehyde
-
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
80.5
acetaldehyde
-
L89A variant of BphI, app. Km-value, pH 8.0, 25°C
81
acetaldehyde
-
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
86.3
acetaldehyde
-
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
49.2
Butyraldehyde
-
L89A variant of BphI, app. Km-value, pH 8.0, 25°C
104
Butyraldehyde
-
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
289
Butyraldehyde
-
L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25°C
323
Butyraldehyde
-
L87A variant of BphI, app. Km-value, pH 8.0, 25°C
0.21
oxaloacetate
-
L87N variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.84
oxaloacetate
-
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.92
oxaloacetate
-
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
6.2
oxaloacetate
-
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
7.4
oxaloacetate
-
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
7.5
oxaloacetate
pH 8.0, 25°C
24.8
pentaldehyde
-
L89A variant of BphI, app. Km-value, pH 8.0, 25°C
33.3
pentaldehyde
-
L87A/L89A variant of BphI, app. Km-value, pH 8.0, 25°C
80.5
pentaldehyde
-
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
16.8
propionaldehyde
-
L87A variant of BphI, app. Km-value, pH 8.0, 25°C
24.4
propionaldehyde
-
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
40.2
propionaldehyde
-
L89A variant of BphI, app. Km-value, pH 8.0, 25°C
54.9
propionaldehyde
-
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
81.2
propionaldehyde
-
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
135.9
propionaldehyde
-
app. Km-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
136
propionaldehyde
-
wild-type BphI, app. Km-value, pH 8.0, 25°C
136
propionaldehyde
-
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
11
pyruvate
-
L89A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
13
pyruvate
-
wild-type BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
14.8
pyruvate
-
steady-state kinetic parameter, pH 8.0 and 25°C
20
pyruvate
-
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
20.2
pyruvate
-
L87A variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
30.2
pyruvate
-
L87N/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
38.2
pyruvate
-
L87W/Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C
additional information
additional information
presence of NAD+, NADP+, NADH or CoA leads to an at least 5fold reduction in the Km for 4-hydroxy-2-oxopentanoate
-
additional information
additional information
-
value not detectable for R16A variant of BphI
-
additional information
additional information
with 4-hydroxy-2-oxopentanoate at 50°C Km-value is not affected
-
additional information
additional information
-
with 4-hydroxy-2-oxopentanoate at 50°C Km-value is not affected
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.043 - 0.17
(4R)-4-hydroxy-2-oxopentanoate
0.089 - 4.07
(4S)-4-hydroxy-2-oxopentanoate
0.63
4-hydroxy-2-oxoheptanoate
pH 8.0, 25°C
0.27 - 3.9
4-hydroxy-2-oxohexanoate
0.08 - 4.3
4-hydroxy-2-oxopentanoate
0.16 - 0.64
Butyraldehyde
0.4
glycolaldehyde
-
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.1 - 1.79
propionaldehyde
additional information
additional information
-
0.043
(4R)-4-hydroxy-2-oxopentanoate
-
L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.098
(4R)-4-hydroxy-2-oxopentanoate
-
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.131
(4R)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.17
(4R)-4-hydroxy-2-oxopentanoate
-
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.089
(4S)-4-hydroxy-2-oxopentanoate
-
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.099
(4S)-4-hydroxy-2-oxopentanoate
-
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.132
(4S)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.171
(4S)-4-hydroxy-2-oxopentanoate
-
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
4
(4S)-4-hydroxy-2-oxopentanoate
-
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
4.07
(4S)-4-hydroxy-2-oxopentanoate
-
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.27
4-hydroxy-2-oxohexanoate
-
G323L mutant, steady state, pH 8.0, 25°C
0.46
4-hydroxy-2-oxohexanoate
-
G323A mutant, steady state, pH 8.0, 25°C
0.5
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
0.94
4-hydroxy-2-oxohexanoate
-
G322L mutant, steady state, pH 8.0, 25°C
1.05
4-hydroxy-2-oxohexanoate
-
G322F mutant, steady state, pH 8.0, 25°C
1.1
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
1.3
4-hydroxy-2-oxohexanoate
-
G323F mutant, steady state, pH 8.0, 25°C
1.9
4-hydroxy-2-oxohexanoate
-
G322A mutant, steady state, pH 8.0, 25°C
2.1
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
2.2
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
3.8
4-hydroxy-2-oxohexanoate
pH 8.0, 25°C
3.9
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
3.9
4-hydroxy-2-oxohexanoate
-
wild-type protein, steady state, pH 8.0, 25°C
0.08
4-hydroxy-2-oxopentanoate
with Ca2+ chloride salt, pH 8.0, 25°C
0.1
4-hydroxy-2-oxopentanoate
-
G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.13
4-hydroxy-2-oxopentanoate
with Mg2+ chloride salt, pH 8.0, 25°C
0.286
4-hydroxy-2-oxopentanoate
-
G322L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.34
4-hydroxy-2-oxopentanoate
-
G323A mutant, steady state, pH 8.0, 25°C
0.45
4-hydroxy-2-oxopentanoate
-
G322F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.57
4-hydroxy-2-oxopentanoate
with Mn2+ chloride salt, pH 8.0, 25°C
0.6
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
0.64
4-hydroxy-2-oxopentanoate
-
G323L mutant, steady state, pH 8.0, 25°C
0.72
4-hydroxy-2-oxopentanoate
-
G323F mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.79
4-hydroxy-2-oxopentanoate
-
wild-type protein, steady state, absence of BphJ cofactors, pH 8.0, 25°C
0.79
4-hydroxy-2-oxopentanoate
with Ni2+ chloride salt, pH 8.0, 25°C
1
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
1
4-hydroxy-2-oxopentanoate
with Co2+ chloride salt, pH 8.0, 25°C
1.06
4-hydroxy-2-oxopentanoate
-
G322F mutant, steady state, pH 8.0, 25°C
1.09
4-hydroxy-2-oxopentanoate
-
G322L mutant, steady state, pH 8.0, 25°C
2.3
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
2.4
4-hydroxy-2-oxopentanoate
-
G322A mutant, steady state, pH 8.0, 25°C
2.4
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
4.07
4-hydroxy-2-oxopentanoate
-
wild-type protein, steady state, pH 8.0, 25°C
4.1
4-hydroxy-2-oxopentanoate
pH 8.0, 25°C
4.1
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
4.3
4-hydroxy-2-oxopentanoate
-
G323F mutant, steady state, pH 8.0, 25°C
0.2
acetaldehyde
-
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.3
acetaldehyde
-
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.59
acetaldehyde
-
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.86
acetaldehyde
-
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.86
acetaldehyde
-
wild-type BphI, app. kcat-value, pH 8.0, 25°C
0.86
acetaldehyde
-
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.94
acetaldehyde
-
L89A variant of BphI, app. kcat-value, pH 8.0, 25°C
0.16
Butyraldehyde
-
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.26
Butyraldehyde
-
L87A variant of BphI, app. kcat-value, pH 8.0, 25°C
0.3
Butyraldehyde
-
L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25°C
0.64
Butyraldehyde
-
L89A variant of BphI, app. kcat-value, pH 8.0, 25°C
0.05
oxaloacetate
-
L87N variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.08
oxaloacetate
-
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.27
oxaloacetate
-
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.4
oxaloacetate
-
L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
16.3
oxaloacetate
-
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
16.5
oxaloacetate
pH 8.0, 25°C
0.13
pentaldehyde
-
L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25°C
0.14
pentaldehyde
-
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.58
pentaldehyde
-
L89A variant of BphI, app. kcat-value, pH 8.0, 25°C
0.1
propionaldehyde
-
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.14
propionaldehyde
-
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.16
propionaldehyde
-
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.29
propionaldehyde
-
L87A variant of BphI, app. kcat-value, pH 8.0, 25°C
0.63
propionaldehyde
-
L89A variant of BphI, app. kcat-value, pH 8.0, 25°C
1.79
propionaldehyde
-
app. kcat-value with pyruvate as carbonyl donor, pH 8.0 and 25°C
1.79
propionaldehyde
-
wild-type BphI, app. kcat-value, pH 8.0, 25°C
1.79
propionaldehyde
-
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.11
pyruvate
-
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.16
pyruvate
-
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.19
pyruvate
-
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.32
pyruvate
-
L87A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
0.64
pyruvate
-
L89A variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
1.04
pyruvate
-
steady-state kinetic parameter, with acetaldehyde as aldehyde donor, pH 8.0 and 25°C
1.2
pyruvate
-
wild-type BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C
additional information
additional information
2 and 5fold increases in kcat observed when BphI reaction is conducted in the presence of NAD+ and NADH
-
additional information
additional information
-
value not detectable for R16A variant of BphI
-
additional information
additional information
with 4-hydroxy-2-oxopentanoate at 50°C turnover number is increased approximately 4fold
-
additional information
additional information
-
with 4-hydroxy-2-oxopentanoate at 50°C turnover number is increased approximately 4fold
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.1 - 14.1
(4R)-4-hydroxy-2-oxopentanoate
0.012 - 44.7
(4S)-4-hydroxy-2-oxopentanoate
1.8
4-hydroxy-2-oxoheptanoate
pH 8.0, 25°C
5.2 - 33.3
4-hydroxy-2-oxohexanoate
0.8 - 46.1
4-hydroxy-2-oxopentanoate
0.00042 - 0.0134
acetaldehyde
0.00016 - 0.0129
Butyraldehyde
0.0037
glycolaldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.00001 - 0.00013
hexaldehyde
0.0005
Isobutyraldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0058 - 2.2
oxaloacetate
0.00004 - 0.0233
pentaldehyde
0.0018 - 0.0175
propionaldehyde
additional information
additional information
-
0.1
(4R)-4-hydroxy-2-oxopentanoate
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.13
(4R)-4-hydroxy-2-oxopentanoate
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
10.4
(4R)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
10.4
(4R)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
14.1
(4R)-4-hydroxy-2-oxopentanoate
-
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.012
(4S)-4-hydroxy-2-oxopentanoate
-
R16K variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.34
(4S)-4-hydroxy-2-oxopentanoate
-
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
0.45
(4S)-4-hydroxy-2-oxopentanoate
-
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
12.6
(4S)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
12.6
(4S)-4-hydroxy-2-oxopentanoate
-
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
13.4
(4S)-4-hydroxy-2-oxopentanoate
-
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C
44.7
(4S)-4-hydroxy-2-oxopentanoate
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
44.7
(4S)-4-hydroxy-2-oxopentanoate
-
wild-type BphI, steady-state kinetic parameter, pH 8.0, 25°C
5.2
4-hydroxy-2-oxohexanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
10.1
4-hydroxy-2-oxohexanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
10.6
4-hydroxy-2-oxohexanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
13.5
4-hydroxy-2-oxohexanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
21.1
4-hydroxy-2-oxohexanoate
pH 8.0, 25°C
33.3
4-hydroxy-2-oxohexanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
0.8
4-hydroxy-2-oxopentanoate
with app. Km-value, with Ca2+ chloride salt, pH 8.0, 25°C
1.6
4-hydroxy-2-oxopentanoate
with app. Km-value, with Mg2+ chloride salt, pH 8.0, 25°C
4.9
4-hydroxy-2-oxopentanoate
single TTHB246, 1 mM MnCl2, pH 8.0, 25°C
7.8
4-hydroxy-2-oxopentanoate
BphI-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
9.3
4-hydroxy-2-oxopentanoate
TTHB246-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
11
4-hydroxy-2-oxopentanoate
with app. Km-value, with Ni2+ chloride salt, pH 8.0, 25°C
13
4-hydroxy-2-oxopentanoate
with app. Km-value, with Mn2+ chloride salt, pH 8.0, 25°C
14.6
4-hydroxy-2-oxopentanoate
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C
18.6
4-hydroxy-2-oxopentanoate
pH 8.0, 25°C
19
4-hydroxy-2-oxopentanoate
with app. Km-value, with Co2+ chloride salt, pH 8.0, 25°C
46.1
4-hydroxy-2-oxopentanoate
BphI-BphJ complex, 1 mM MnCl2, pH 8.0, 25°C
0.00042
acetaldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00049
acetaldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0025
acetaldehyde
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0043
acetaldehyde
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0069
acetaldehyde
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0117
acetaldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0134
acetaldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0134
acetaldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0134
acetaldehyde
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00016
Butyraldehyde
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00031
Butyraldehyde
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00079
Butyraldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00104
Butyraldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0015
Butyraldehyde
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00219
Butyraldehyde
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0022
Butyraldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0022
Butyraldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0129
Butyraldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00001
hexaldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00001
hexaldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0001
hexaldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00013
hexaldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0058
oxaloacetate
-
L87W variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.064
oxaloacetate
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.098
oxaloacetate
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.254
oxaloacetate
-
L87N variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.53
oxaloacetate
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
2.2
oxaloacetate
pH 8.0, 25°C
2.2
oxaloacetate
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00004
pentaldehyde
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.000051
pentaldehyde
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00033
pentaldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00047
pentaldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.00047
pentaldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.00047
pentaldehyde
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0017
pentaldehyde
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0038
pentaldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0233
pentaldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0018
propionaldehyde
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.002
propionaldehyde
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0057
propionaldehyde
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.00673
propionaldehyde
-
L87A/L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0132
propionaldehyde
-
kcat(app)/Km(app) with pyruvate as carbonyl donor, pH 8.0 and 25°C
0.0132
propionaldehyde
-
wild-type BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0132
propionaldehyde
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0156
propionaldehyde
-
L89A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0175
propionaldehyde
-
L87A variant of BphI, app. kcat/Km-value, pH 8.0, 25°C
0.0036
pyruvate
-
L87N/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0043
pyruvate
-
L87W/Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0084
pyruvate
-
Y290F variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0159
pyruvate
-
L87A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0587
pyruvate
-
L89A variant of BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
0.0915
pyruvate
-
wild-type BphI, app. kcat/Km-value, steady-state kinetic parameter, pH 8.0, 25°C
additional information
additional information
highest catalytic efficiency of BphI occurs when NADH is present, which is 25fold higher than the value obtained without nucleotides
-
additional information
additional information
-
value not detectable for R16A variant of BphI
-
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