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IUBMB CommentsThe insect enzyme is involved in the conversion of sitosterol into cholesterol.
The expected taxonomic range for this enzyme is: Manduca sexta
Synonyms
lyase, fucosterol epoxide, more
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lyase, fucosterol epoxide
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(24R,241R)-fucosterol epoxide = desmosterol + acetaldehyde
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reversal of an aldol condensation
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(24R,241R)-fucosterol-epoxide acetaldehyde-lyase (desmosterol-forming)
The insect enzyme is involved in the conversion of sitosterol into cholesterol.
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(24R,241R)-fucosterol epoxide
desmosterol + acetaldehyde
(24RS,28RS)-fucosterol epoxide
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Substrates: -
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(24S,28S)-fucosterol epoxide
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Substrates: -
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(24R,241R)-fucosterol epoxide
desmosterol + acetaldehyde
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Substrates: -
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(24R,241R)-fucosterol epoxide
desmosterol + acetaldehyde
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Substrates: best substrate
Products: demonstrated in crude extract
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(24R,241R)-fucosterol epoxide
desmosterol + acetaldehyde
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Substrates: -
Products: -
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Ca2+
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10 mM: 16% of maximal activity
chymotrypsin
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0.05 mg/ml: 57% of maximal activity
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D-glucose
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200 mM: 42% of maximal activity
EDTA
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10 mM: 66% of maximal activity
Fe2+
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10 mM: 18% of maximal activity
Fe3+
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10 mM: 26% of maximal activity
glutathione
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oxidized, 10 mM: 58% of maximal activity, reduced: 10 mM, 89% of maximal activity
imidazole
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10 mM: 99% of maximal activity
K+
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10 mM: 71% of maximal activity
Lubrol Px
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1% (w/v): complete inhibition
Mg2+
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10 mM: 75% of maximal activity
N-ethylmaleimide
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10 mM: 77% of maximal activity
NAD+
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10 mM: 95% of maximal activity
phenylmethylsulfonyl fluoride
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10 mM: 63% of maximal activity
Staphylococcus aureus V8 protease
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0.05 mg/ml: 20% of maximal activity
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sucrose
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200 mM: 73% of maximal activity
Triton X-100
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1% (w/v): 12% of maximal activity
Zn2+
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10 mM: 9% of maximal activity
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2-mercaptoethanol
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10 mM: 103% of maximal activity, slight activation
NADH
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10 mM: 139% of maximal activity, activation
NADP+
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10 mM: 109% of maximal activity, activation
NADPH
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10 mM: 106% of maximal activity, slight activation
p-hydroxymercuribenzoate
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1 mM: 102% of maximal activity, slight activation
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0.0007
(24RS,28RS)-fucosterol epoxide
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37°C
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broad range of activity
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tobacco hornworm
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brenda
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brenda
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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no activity is lost in a single freeze-thaw cycle
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-80°C, no activity is lost in a single freeze-cycle
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larval tobacco hornworm, partial
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Prestwich, G.D.; Angelastro, M.; de Palma, A.; Perino, M.A.
Fucosterol epoxide lyase of insects: synthesis of labeled substrates and development of a partition assay
Anal. Biochem.
151
315-326
1985
Manduca sexta
brenda
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