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EC Tree
IUBMB Comments Also acts on methylated lombricines such as thalassemine; the specificity varies with the source species.
The enzyme appears in viruses and cellular organisms
Synonyms
lombricine kinase,
more
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guanidinethylphosphoserine kinase
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kinase (phosphorylating), lombricine
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kinase, lombricine (phosphorylating)
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MiLK
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mitochondrial enzyme
LK
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ATP + lombricine = ADP + N-phospholombricine
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phospho group transfer
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ATP:lombricine N-phosphotransferase
Also acts on methylated lombricines such as thalassemine; the specificity varies with the source species.
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ATP + D-lombricine
ADP + phospholombricine
ATP + guanidinoethyl phosphate
ADP + N-phospho-guanidinoethyl phosphate
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15% of the activity with D-lombricine
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?
ATP + L-lombricine
ADP + phospholombricine
ATP + L-thalassemine
ADP + N-L-thalassemine phosphate
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i.e. guanidinoethylphospho-O-(alpha-N,N-dimethyl)serine, 88% of the activity with D-lombricine
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?
ATP + lombricine
ADP + N-phospholombricine
ATP + lombricine
ADP + phospholombricine
ATP + taurocyamine
ADP + N-taurocyamine phosphate
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44% of the activity with D-lombricine
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?
dADP + phospholombricine
dATP + lombricine
Megascolides cameroni
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at 16% of the activity with ADP
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?
ATP + D-lombricine
ADP + phospholombricine
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D-isomer, 100%, and L-isomer, 176%, are reactive
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?
ATP + D-lombricine
ADP + phospholombricine
Megascolides cameroni
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r
ATP + L-lombricine
ADP + phospholombricine
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D-isomer, 100%, and L-isomer, 176%, are reactive
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?
ATP + L-lombricine
ADP + phospholombricine
Megascolides cameroni
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r
ATP + lombricine
ADP + N-phospholombricine
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?
ATP + lombricine
ADP + N-phospholombricine
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?
ATP + lombricine
ADP + N-phospholombricine
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?
ATP + lombricine
ADP + N-phospholombricine
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?
ATP + lombricine
ADP + N-phospholombricine
Stylaria sp.
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?
ATP + lombricine
ADP + N-phospholombricine
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?
ATP + lombricine
ADP + N-phospholombricine
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?
ATP + lombricine
ADP + N-phospholombricine
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?
ATP + lombricine
ADP + N-phospholombricine
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?
ATP + lombricine
ADP + phospholombricine
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?
ATP + lombricine
ADP + phospholombricine
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ATP + lombricine
ADP + phospholombricine
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D-isomer, 100%, and L-isomer, 176%, are reactive
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?
ATP + lombricine
ADP + phospholombricine
Megascolides cameroni
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i.e. guanidinoethylphospho-O-serine
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r
ATP + lombricine
ADP + phospholombricine
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?
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ATP + lombricine
ADP + phospholombricine
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?
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Ca2+
Megascolides cameroni
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5 mM, slight activation in both directions
Co2+
Megascolides cameroni
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5 mM, slight activation in both directions
Mn2+
Megascolides cameroni
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5 mM, activation in both directions
additional information
Megascolides cameroni
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not activated by Ni2+, Cu2+, Fe2+, Fe3+, Al3+, Sn2+, Ba2+, Cd2+, Zn2+, Be2+
Mg2+
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enzyme contains one binding site for MgADP-
Mg2+
Megascolides cameroni
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5 mM, activation in both directions
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2,4-dinitrofluorobenzene
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p-hydroxymercuribenzoate
Megascolides cameroni
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0.0001 mM, complete inhibition
NEM
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NEM
Megascolides cameroni
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0.3 mM, complete inhibition
o-Iodosobenzoate
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o-Iodosobenzoate
Megascolides cameroni
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0.3 mM, complete inhibition
Phenyl iodoacetate
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Phenyl iodoacetate
Megascolides cameroni
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1 mM, complete inhibition
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9.1
D-lombricine
Megascolides cameroni
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pH 8.6, 30°C
11.7
L-lombricine
Megascolides cameroni
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pH 8.6, 30°C
7.4
phospholombricine
Megascolides cameroni
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pH 8.6, 30°C
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31
Megascolides cameroni
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D-lombricine
49
Megascolides cameroni
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L-lombricine
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7.2
Megascolides cameroni
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synthesis of ATP
8.6
Megascolides cameroni
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phosphorylation of lombricine
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6.5 - 8
Megascolides cameroni
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pH 6.5: about 85% of maximal activity, pH 8.0: about 50% of maximal activity, synthesis of ATP
7.5 - 9.5
Megascolides cameroni
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pH 7.5: about 65% of maximal activity, pH 9.5: about 45% of maximal activity, phosphorylation of lombricine
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7.6
calculated from amino acid sequence
7.7
calculated from amino acid sequence
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brenda
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brenda
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UniProt
brenda
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UniProt
brenda
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brenda
Megascolides cameroni
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brenda
Stylaria sp.
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UniProt
brenda
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UniProt
brenda
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UniProt
brenda
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UniProt
brenda
California blackworm
UniProt
brenda
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UniProt
brenda
sludge worm
UniProt
brenda
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brenda
Megascolides cameroni
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brenda
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brenda
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brenda
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KLOM_EISFE
371
0
41781
Swiss-Prot
other Location (Reliability: 3 )
KLOM_LUMTE
15
0
1565
Swiss-Prot
other Location (Reliability: 1 )
I0E0A7_9ANNE
368
0
41860
TrEMBL
other Location (Reliability: 2 )
I0E0A8_9ANNE
371
0
41876
TrEMBL
other Location (Reliability: 2 )
B0FYX1_9ANNE
368
0
41437
TrEMBL
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C8CG36_TUBTU
368
0
41649
TrEMBL
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Q8T6T7_URECA
366
0
40941
TrEMBL
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40941
x * 40941, calculation from nucleotide sequence
41437
x * 41437, calculated from amino acid sequence
41649
x * 41649, calculated from amino acid sequence
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dimer
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two non-identical subunits, Sepharose-mercurial chromatography
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x * 41437, calculated from amino acid sequence
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x * 41649, calculated from amino acid sequence
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x * 40941, calculation from nucleotide sequence
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substrate-free enzyme or in complex with ADP, vapor diffusion method, using 15 mM bis-Tris, 0.2 M NaNO3, 1 mM dithiothreitol, and 20% (w/v) PEG 3350MME, pH 6.8
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K95Y
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the mutation produces a dramatic change in guanidine substrate specificity, resulting in a shift of substrate specificity from lombricine to taurocyamine, the K95Y mutant functions not as lombricine kinase but as taurocyamine kinase
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freeze-drying of preparations in non-volatile buffers causes almost 40% loss of activity. Freezing and thawing at pH 8.6 in N-ethylmorpholine-hydrochloric acid buffer produces complete inactivation
Megascolides cameroni
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0°C, 0.033 M phosphate buffer, pH 7, or 0.05 M Tris-HCl buffer, pH 7.5, or 0.01 M glycylglycine buffer, pH 7, several weeks stable, or in 75% saturated ammonium sulfate solution, pH 8 stable for several months
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2°C, pH 7.5, 50% v/v glycerol, several months stable
Megascolides cameroni
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Megascolides cameroni
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DEAE column chromatography, ADP-agarose column chromatography, and Superdex 200 gel filtration
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expressed in Escherichia coli
expressed in Escherichia coli TOP 10 cells
expression in Escherichia coli
expressed in Escherichia coli TOP 10 cells
expressed in Escherichia coli TOP 10 cells
Stylaria sp.
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Kassab, R.; Pradel, L.A.; van Thoai, N.
ATP:taurocyamine and ATP:lombricine phosphotransferases. Purification and study of SH groups
Biochim. Biophys. Acta
99
397-405
1965
Lumbricus terrestris
brenda
Der Terrossian, E.; Pradel, L.A.; Kassab, R.; Desvages, G.
Separation of the two non-identical subunits of lombricine kinase from Lumbricus terrestris muscle by chromatography on sepharose-mercurial. Isolation of the tryptic peptide containing its essential thiol group
Eur. J. Biochem.
45
243-251
1974
Lumbricus terrestris
brenda
Gaffney, T.J.; Rosenberg, H.; Ennor, A.H.
The purification and properties of adenosine triphosphate-lombricine phosphotransferase
Biochem. J.
90
170-176
1964
Megascolides cameroni
brenda
van Thoai, N.; Robin, Y.; Guillou, Y.
A new phosphagen, N-phosphorylguanidinoethylphospho-O-(alpha-N,N-dimethyl)serine (phosphothalassemine)
Biochemistry
11
3890-3895
1972
Lumbricus terrestris
brenda
Ross Ellington, W.; Bush, J.
Cloning and expression of a lombricine kinase from an echiuroid worm: Insights into structural correlates of substrate specificity
Biochem. Biophys. Res. Commun.
291
939-944
2002
Urechis caupo (Q8T6T7), Urechis caupo
brenda
Tanaka, K.; Uda, K.; Shimada, M.; Takahashi, K.I.; Gamou, S.; Ellington, W.R.; Suzuki, T.
Evolution of the cytoplasmic and mitochondrial phosphagen kinases unique to annelid groups
J. Mol. Evol.
65
616-625
2007
Eisenia fetida
brenda
Uda, K.; Kuwasaki, A.; Shima, K.; Matsumoto, T.; Suzuki, T.
The role of Arg-96 in Danio rerio creatine kinase in substrate recognition and active center configuration
Int. J. Biol. Macromol.
44
413-418
2009
Danio rerio
brenda
Doumen, C.
cDNA identification, comparison and phylogenetic aspects of lombricine kinase from two oligochaete species
Comp. Biochem. Physiol. B
156
137-143
2010
Lumbriculus variegatus (B0FYX1), Tubifex tubifex (C8CG36), Tubifex tubifex
brenda
Doumen, C.
Variable intron/exon structure in the oligochaete lombricine kinase gene
Gene
505
276-282
2012
Lumbriculus variegatus (B0FYX1), Lumbriculus variegatus, Tubifex tubifex (C8CG36), Tubifex tubifex, Stylaria sp. (I0E0A7), Enchytraeus sp. (I0E0A8), Enchytraeus sp., Enchytraeus sp. CD-2012 (I0E0A8), Stylaria sp. CD-2012 (I0E0A7)
brenda
Bush, D.J.; Kirillova, O.; Clark, S.A.; Davulcu, O.; Fabiola, F.; Xie, Q.; Somasundaram, T.; Ellington, W.R.; Chapman, M.S.
The structure of lombricine kinase: implications for phosphagen kinase conformational changes
J. Biol. Chem.
286
9338-9350
2011
Urechis caupo (Q8T6T7), Urechis caupo
brenda
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