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EC Tree
IUBMB Comments The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of the bacterium Escherichia coli serotype O86.
The expected taxonomic range for this enzyme is: Escherichia coli
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WbnI
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UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol = UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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UDP-alpha-D-galactose:alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol alpha-1,3-galactosyltransferase
The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of the bacterium Escherichia coli serotype O86.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
UDP + alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-OMe
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UDP-alpha-D-galactose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
alpha-D-Gal-(1->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
additional information
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UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
UDP + alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
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UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
UDP + alpha-D-Gal-(1->3)-[alpha-L-Fuc-(1->2)]-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
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UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-OMe
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30% of the activity compared to alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
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UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-OMe
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30% of the activity compared to alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
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UDP-alpha-D-galactose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
alpha-D-Gal-(1->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
12% of the activity compared to alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
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UDP-alpha-D-galactose + beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
alpha-D-Gal-(1->3)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
12% of the activity compared to alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-OMe
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additional information
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the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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additional information
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WbnI prefers terminal fucosylated sugar structures to nonfucosylated ones
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additional information
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the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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additional information
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WbnI prefers terminal fucosylated sugar structures to nonfucosylated ones
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
additional information
?
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UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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?
UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol
biosynthesis of the O-polysaccharide is initiated with sequential assembly of repeating units on the cytoplasmic face of the inner membrane by glycosyltransferases WbnH, WbnJ, WbnK and WbnI. The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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additional information
?
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the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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-
?
additional information
?
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the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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UniProt
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UniProt
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UniProt
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malfunction
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wbnI gene is replaced by a chloramphenicol acetyltransferase gene using the RED recombination system of phage lambda. Inactivated the wbnI gene shows that the mutant strain produces a different polysaccharide without the a-(1->3)-linked Galp side chain. The yield of the polysaccharide is substantially lower than the one produced by the wild-type strain
malfunction
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wbnI gene is replaced by a chloramphenicol acetyltransferase gene using the RED recombination system of phage lambda. Inactivated the wbnI gene shows that the mutant strain produces a different polysaccharide without the a-(1->3)-linked Galp side chain. The yield of the polysaccharide is substantially lower than the one produced by the wild-type strain
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physiological function
the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
physiological function
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the enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of Escherichia coli serotype O86
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WBNI_ECOLX
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0
28134
Swiss-Prot
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expressed in Escherichia coli as a GST-tagged fusion protein
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synthesis
stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Biosynthetic pathway of B antigen is the same in Escherichia coli O86 as in humans, thus providing an attractive way of synthesizing blood group B antigen with microbial glycosyltransferases
synthesis
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stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Biosynthetic pathway of B antigen is the same in Escherichia coli O86 as in humans, thus providing an attractive way of synthesizing blood group B antigen with microbial glycosyltransferases
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Yi, W.; Zhu, L.; Guo, H.; Li, M.; Li, J.; Wang, P.G.
Formation of a new O-polysaccharide in Escherichia coli O86 via disruption of a glycosyltransferase gene involved in O-unit assembly
Carbohydr. Res.
341
2254-2260
2006
Escherichia coli, Escherichia coli O86
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Yi, W.; Shao, J.; Zhu, L.; Li, M.; Singh, M.; Lu, Y.; Lin, S.; Li, H.; Ryu, K.; Shen, J.; Guo, H.; Yao, Q.; Bush, C.A.; Wang, P.G.
Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide
J. Am. Chem. Soc.
127
2040-2041
2005
Escherichia coli (Q5JBG6), Escherichia coli O86 (Q5JBG6)
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Woodward, R.; Yi, W.; Li, L.; Zhao, G.; Eguchi, H.; Sridhar, P.R.; Guo, H.; Song, J.K.; Motari, E.; Cai, L.; Kelleher, P.; Liu, X.; Han, W.; Zhang, W.; Ding, Y.; Li, M.; Wang, P.G.
In vitro bacterial polysaccharide biosynthesis: defining the functions of Wzy and Wzz
Nat. Chem. Biol.
6
418-423
2010
Escherichia coli (Q5JBG6), Escherichia coli O86:B7 (Q5JBG6)
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2.4.1.309 UDP-Gal:alpha-L-Fuc-1,2-beta-Gal-1,3-alpha-GalNAc-1,3-alpha-GalNAc-diphosphoundecaprenol alpha-1,3-galactosyltransferase
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