We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of the bacterium Shigella boydii B14. The activity is stimulated by Mn2+ or to a lesser extent by Mg2+, Ca2+, Ni2+ or Pb2+.
The enzyme appears in viruses and cellular organisms
Synonyms
UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-galactosyltransferase, UDP-Gal:GlcNAc-R 1,4-Gal-transferase, WfeD,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-galactosyltransferase
UDP-Gal:GlcNAc-R 1,4-Gal-transferase
UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-galactosyltransferase
-
-
UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-galactosyltransferase
-
-
-
UDP-Gal:GlcNAc-R 1,4-Gal-transferase
-
-
UDP-Gal:GlcNAc-R 1,4-Gal-transferase
-
-
-
WfeD
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactose:N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol beta-1,4-galactosyltransferase
The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of the bacterium Shigella boydii B14. The activity is stimulated by Mn2+ or to a lesser extent by Mg2+, Ca2+, Ni2+ or Pb2+.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol
-
the synthetic acceptor substrate D-GlcNAc-alpha-PP-(CH2)11-O-Ph is an amphipathic molecule with similarity to the endogenous substrate D-GlcNAc-alpha-diphospho-ditrans,octacis-undecaprenol. The enzyme requires diphosphate in the acceptor substrate, Strict specificity for UDP-D-galactose as donor substrate
-
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphosphononanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphononanol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-phospho-phenoxyundecanol
-
synthetic acceptor. 11% of the activity with N-acetyl-D-glucosaminyl-alpha-diphospho-phenoxyundecanol
-
-
?
additional information
?
-
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14
-
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14
-
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
-
the enzyme requires diphosphate in the acceptor substrate
-
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexadecanol
-
the enzyme requires diphosphate in the acceptor substrate
-
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
-
the enzyme requires diphosphate in the acceptor substrate
-
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-hexanol
-
the enzyme requires diphosphate in the acceptor substrate
-
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphosphononanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphononanol
-
the enzyme requires diphosphate in the acceptor substrate
-
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphosphononanol
UDP + beta-D-Gal-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphosphononanol
-
the enzyme requires diphosphate in the acceptor substrate
-
-
?
additional information
?
-
-
no activity with: alpha- and beta-anomers of GlcNAc-benzyl, beta-anomers of GlcNBu-benzyl, GlcNAc-naphthyl, GlcNAc-isoquinolinyl, as well as GlcNAc-alpha-OCOCH2-COO(CH2)11-OPh and alpha- and beta-anomers of GlcNAc-(CH2)11-OPh
-
-
?
additional information
?
-
-
no activity with: alpha- and beta-anomers of GlcNAc-benzyl, beta-anomers of GlcNBu-benzyl, GlcNAc-naphthyl, GlcNAc-isoquinolinyl, as well as GlcNAc-alpha-OCOCH2-COO(CH2)11-OPh and alpha- and beta-anomers of GlcNAc-(CH2)11-OPh
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14
-
-
?
UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
UDP + beta-D-Gal-(1->4)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
the O-antigen in the outer membrane of Gram-negative bacteria contains many repeats of an oligosaccharide unit. WfeD catalyzes a step of the repeating-unit assembly in Shigella boydii B14
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ca2+
-
5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
Mg2+
-
5 mM, highest stimulation of activityN in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
Mn2+
-
5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
Ni2+
-
5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
Pb2+
-
5 mM, highest stimulation of activity in the presence of Mn2+. Ca2+, Pb2+, Ni2+ and Mg2+ are also effective
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4-hydroxyphenylglyoxal
-
0.2 mM, 50% inhibition
5,5'-dithio-bis-2-nitrobenzoic acid
-
0.2 mM, 85% inhibition
diethyl dicarbonate
-
0.2 mM 31% inhibition
Triton X-100
-
as the concentration of Triton X-100 increases from 0 to 0.5% in the reaction mixture, the activity of the enzyme decreases but is maintained at about 60% of the original activity, up to 6% Triton X-100
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-mercaptoethanol
-
1-30 mM, 4fold-stimulation
dithiothreitol
-
0.1-3 mM, about 5fold stimulation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.1
N-acetyl-alpha-D-glucosaminyl-diphospho-phenoxyundecanol
-
pH 7, 37°C
0.25
UDP-alpha-D-galactose
-
pH 7, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
WFED_SHIBO
237
0
27941
Swiss-Prot
-
A0A0P0FS24_9BACE
242
0
27799
TrEMBL
-
A0A5E7S8K8_PSEFL
234
0
26385
TrEMBL
-
A0A6F9ZA27_9BACT
237
0
27505
TrEMBL
-
A0A5P3ATF8_PHOVU
237
0
27777
TrEMBL
-
A0A2K9JU97_9GAMM
237
0
27388
TrEMBL
-
A0A0Q0MI37_9GAMM
236
0
27396
TrEMBL
-
A0A5E6V051_PSEFL
235
0
26574
TrEMBL
-
A0A5E7HVZ9_PSEFL
235
0
26677
TrEMBL
-
A0A397WEF0_PHOVU
237
0
27717
TrEMBL
-
A0A158C5E5_9BURK
232
0
26531
TrEMBL
-
A0A158C1A0_9BURK
243
0
27824
TrEMBL
-
A0A662ZZM3_PHOVU
237
0
27593
TrEMBL
-
A0A5E6PP01_PSEFL
235
0
26906
TrEMBL
-
A0A1Q6J3S5_PHOVU
246
0
28840
TrEMBL
-
A0A5E6ZKR2_PSEFL
234
0
26338
TrEMBL
-
A0A663BN69_9ENTR
225
0
26051
TrEMBL
-
A0A5E7IM69_PSEFL
234
0
26334
TrEMBL
-
A0A5E7JQU8_PSEFL
235
0
26642
TrEMBL
-
A0A5E7AJC0_PSEFL
235
0
26467
TrEMBL
-
A0A7M1VWL5_VIBPH
247
0
28736
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 30000, SDS-PAGE, calculated from sequence
?
-
x * 30000, SDS-PAGE, calculated from sequence
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D99A
-
highly active mutant enzyme
E101A
-
mutant exhibits minimal enzyme activity, greatly reduced enzyme activity in the presence of either Mn2+ or Pb2+
E103A
-
highly active mutant enzyme
K211A
-
greatly reduced activity, 60fold higher activity in the presence of Pb2+ than in the presence of Mn2+
R210A
-
active mutant enzyme
R212A
-
active mutant enzyme
D99A
-
highly active mutant enzyme
-
E101A
-
mutant exhibits minimal enzyme activity, greatly reduced enzyme activity in the presence of either Mn2+ or Pb2+
-
E103A
-
highly active mutant enzyme
-
R210A
-
active mutant enzyme
-
R212A
-
active mutant enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4°C, the crude enzyme preparation retains at least 60% of the initial activity after storage for up to 9 weeks
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Xu, C.; Liu, B.; Hu, B.; Han, Y.; Feng, L.; Allingham, J.S.; Szarek, W.A.
Wang, L.; Brockhausen, I.: Biochemical characterization of UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-Galactosyltransferase WfeD, a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating-unit synthesis
J. Bacteriol.
193
449-459
2010
Shigella boydii, Shigella boydii B14
brenda
Select items on the left to see more content.
html completed