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UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine = UDP + kanamycin A
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine = UDP + kanamycin X
UDP-alpha-D-kanosamine + neamine = UDP + kanamycin B
UDP-alpha-D-kanosamine + paromamine = UDP + kanamycin C
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine = UDP + kanamycin A

(1)
-
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine = UDP + kanamycin A
-
-
-
-
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine = UDP + kanamycin X

(4)
-
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine = UDP + kanamycin X
-
-
-
-
UDP-alpha-D-kanosamine + neamine = UDP + kanamycin B

(2)
-
UDP-alpha-D-kanosamine + neamine = UDP + kanamycin B
-
-
-
-
UDP-alpha-D-kanosamine + paromamine = UDP + kanamycin C

(3)
-
UDP-alpha-D-kanosamine + paromamine = UDP + kanamycin C
-
-
-
-
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UDP-alpha-D-glucose + paromamine
UDP + ?
paromamine i.e. (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside. UDP-alpha-D-kanosamine is a better glycosyl donor than UDP-alpha-D-glucose
-
-
?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine
UDP + kanamycin A
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine
UDP + kanamycin X
UDP-alpha-D-kanosamine + neamine
UDP + kanamycin B
UDP-alpha-D-kanosamine + paromamine
UDP + kanamycin C
additional information
?
-
the enzyme can also accept UDP-alpha-D-glucose with lower efficiency
-
-
?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine

UDP + kanamycin A
the enzyme is involved in the biosynthetic pathway of kanamycins
-
-
?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine
UDP + kanamycin A
UDP-alpha-D-kanosamine i.e. uridine 5'-[3-(3-amino-3-deoxy-alpha-D-glucopyranosyl) diphosphate]
kanamycin A i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy-alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside
-
?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine

UDP + kanamycin X
-
kanamycin X i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-(alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside
-
?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine
UDP + kanamycin X
the enzyme is involved in the biosynthetic pathway of kanamycins
-
-
?
UDP-alpha-D-kanosamine + neamine

UDP + kanamycin B
the enzyme is involved in the biosynthetic pathway of kanamycins
-
-
?
UDP-alpha-D-kanosamine + neamine
UDP + kanamycin B
neamine i.e. (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside
kanamycin B i.e. (1R,2S,3S,4R,6S)-4,6-diamino-3-(3-amino-3-deoxy-alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 2,6-diamino-2,6-dideoxy-alpha-D-glucopyranoside
-
?
UDP-alpha-D-kanosamine + paromamine

UDP + kanamycin C
the enzyme is involved in the biosynthetic pathway of kanamycins
-
-
?
UDP-alpha-D-kanosamine + paromamine
UDP + kanamycin C
paromamine i.e. (1R,2R,3S,4R,6S)-4,6-diamino-2,3-dihydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside
kanamycin C i.e. (1R,2S,3S,4R,6S)-4,6-diamino-3-(3-amino-3-deoxy-alpha-D-glucopyranosyloxy)-2-hydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyneamine
UDP + kanamycin A
the enzyme is involved in the biosynthetic pathway of kanamycins
-
-
?
UDP-alpha-D-kanosamine + 2'-deamino-2'-hydroxyparomamine
UDP + kanamycin X
the enzyme is involved in the biosynthetic pathway of kanamycins
-
-
?
UDP-alpha-D-kanosamine + neamine
UDP + kanamycin B
the enzyme is involved in the biosynthetic pathway of kanamycins
-
-
?
UDP-alpha-D-kanosamine + paromamine
UDP + kanamycin C
the enzyme is involved in the biosynthetic pathway of kanamycins
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.26
UDP-alpha-D-glucose
37°C, pH not specified in the publication, cosubstrate: paromamine
0.03
UDP-alpha-D-kanosamine
37°C, pH not specified in the publication, cosubstrate: paromamine
0.05
paromamine

37°C, pH not specified in the publication, cosubstrate: UDP-alpha-D-kanosamine
0.25
paromamine
37°C, pH not specified in the publication, cosubstrate: UDP-alpha-D-glucose
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Park, J.; Park, S.; Nepal, K.; Han, A.; Ban, Y.; Yoo, Y.; Kim, E.; Kim, E.; Kim, D.; Sohng, J.; Yoon, Y.
Discovery of parallel pathways of kanamycin biosynthesis allows antibiotic manipulation
Nat. Chem. Biol.
7
843-852
2011
Streptomyces kanamyceticus (Q65CC7)
brenda
Kudo, F.; Sucipto, H.; Eguchi, T.
Enzymatic activity of a glycosyltransferase KanM2 encoded in the kanamycin biosynthetic gene cluster
J. Antibiot.
62
707-710
2009
Streptomyces kanamyceticus
brenda