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IUBMB Comments One of three specific glucosyltransferases in pea (Pisum sativum ) thatsuccessively add a β-D -glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O -sophoroside and then the 3-O -sophorotrioside (see also EC 2.4.1.91 flavonol 3-O -glucosyltransferase, and EC 2.4.1.239 flavonol-3-O -glucoside glucosyltransferase).
Synonyms
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UDP-glucose:glucosyltransferase
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UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucosyl-(1->2)-beta-D-glucoside
UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucosyl-(1->2)-beta-D-glucoside
one of three specific glucosyltransferases in pea, Pisum sativum, that successively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside, see also EC 2.4.1.91 flavonol 3-O-glucosyltransferase, and EC 2.4.1.239 flavonol-3-O-glucoside glucosyltransferase
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UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside = UDP + a flavonol 3-O-beta-D-glucosyl-(1->2)-beta-D-glucosyl-(1->2)-beta-D-glucoside
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transfer of glycosyl group
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MetaCyc
kaempferol triglucoside biosynthesis, quercetin triglucoside biosynthesis
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UDP-glucose:flavonol-3-O-beta-D-glucosyl-(1->2)-beta-D-glucoside 2'''-O-beta-D-glucosyltransferase
One of three specific glucosyltransferases in pea (Pisum sativum) thatsuccessively add a beta-D-glucosyl group first to O-3 of kaempferol, and then to O-2 of the previously added glucosyl group giving the 3-O-sophoroside and then the 3-O-sophorotrioside (see also EC 2.4.1.91 flavonol 3-O-glucosyltransferase, and EC 2.4.1.239 flavonol-3-O-glucoside glucosyltransferase).
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TDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
TDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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Substrates: 3fold lower rate than with UDP-glucose as glucosyl donor
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UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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Substrates: highly specific enzyme, specific for flavonol-3-O-diglucosides, 3fold higher rate than with TDP-glucose as glucosyl donor
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UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP-glucose + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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Substrates: 32% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
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UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
additional information
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UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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Substrates: -
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UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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Substrates: best substrate
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UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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Substrates: -
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UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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Substrates: 14% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
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additional information
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Substrates: involved in the biosynthesis of flavonol triglucosides
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additional information
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Substrates: one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides
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additional information
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Substrates: glucosylation of kaempferol and quercetin in the 3-position to form the 3-O-triglucoside, reaction proceeds from the aglycone via the mono- and diglucoside intermediates, this reaction sequence is probably associated with three distinct enzyme activities
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additional information
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Substrates: no glucosylation of pelargonidin, naringenin, naringin and p-coumaric acid by the three flavonol:glucosyltransferases, not: ADP-glucose, GDP-glucose, kaempferol, kaempferol monoglucoside, quercetin-3-rutinoside
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additional information
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additional information
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Substrates: involved in the biosynthesis of flavonol triglucosides
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additional information
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Substrates: one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides
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additional information
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no cofactor requirement
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additional information
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not activated by divalent cations, 0.1-10 mM
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UDP
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product inhibition, 0.25 mM, 50% inhibition
additional information
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not inhibited by divalent cations, 0.1-10 mM, EDTA, EGTA, no substrate inhibition
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0.008
kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
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pH 7.3, 30°C
0.8
UDP-glucose
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pH 7.3, 30°C
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additional information
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7.6
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in 0.2 M Tris-HCl buffer, two pH optima, equally active in 0.2 M Tris-HCl and 0.2 M glycine-NaOH buffer
8.3
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in 0.2 M glycine-NaOH buffer, two pH optima, equally active in 0.2 M Tris-HCl and 0.2 M glycine-NaOH buffer
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var. Alaska
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brenda
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young leaf tissues have much higher levels of glucosyltransferase activity than the petioles and internodes
brenda
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young, light-grown, much higher levels of glucosyltransferase activity in young leaf tissues than in petioles and internodes
brenda
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light-grown, one week old
brenda
Highest Expressing Human Cell Lines
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-15°C or room temperature, partially purified enzyme, 36 h, 80% loss of activity
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30.7fold, separation of three distinct flavonol:glucosyltransferases
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Jourdan, P.S.; Mansell, R.L.
Isolation and partial characterization of three glucosyl transferases involved in the biosynthesis of flavonol triglucosides in Pisum sativum L
Arch. Biochem. Biophys.
213
434-443
1982
Lathyrus oleraceus
brenda
Shute, J.L.; Jourdan, P.S.; Mansell, R.L.
UDP-glucose:glucosyltransferase activity involved in the biosynthesis of flavonol triglucosides in Pisum sativum L. seedlings
Z. Naturforsch. C
34
738-741
1979
Lathyrus oleraceus
brenda
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