Any feedback?
No. of entries
Information on EC 2.4.1.194 - 4-hydroxybenzoate 4-O-beta-D-glucosyltransferase
for references in articles please use BRENDA:EC2.4.1.194
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 2 Transferases
2.4 Glycosyltransferases
2.4.1 Hexosyltransferases
2.4.1.194 4-hydroxybenzoate 4-O-beta-D-glucosyltransferase
The enzyme appears in viruses and cellular organisms
showSynonyms
HBA glucosyltransferase, p-hydroxybenzoate glucosyltransferase, PHB glucosyltransferase, PHB-O-glucosyltransferase, UDP-glucose:4-(beta-D-glucopyranosyloxy)benzoic acid glucosyltransferase, uridine diphosphoglucose-4-hydroxybenzoate glucosyltransferase, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
HBA glucosyltransferase
-
-
-
-
p-hydroxybenzoate glucosyltransferase
-
-
-
-
PHB glucosyltransferase
PHB-O-glucosyltransferase
-
-
-
-
UDP-glucose:4-(beta-D-glucopyranosyloxy)benzoic acid glucosyltransferase
-
-
-
-
uridine diphosphoglucose-4-hydroxybenzoate glucosyltransferase
-
-
-
-
PHB glucosyltransferase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + 4-hydroxybenzoate = UDP + 4-(beta-D-glucosyloxy)benzoate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:4-hydroxybenzoate 4-O-beta-D-glucosyltransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
120860-68-6
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDPglucose + 4-nitrophenol
UDP + 4-nitrophenyl O-beta-D-glucoside
-
Substrates: -
Products: -
Products: -
?
UDPglucose + 4-hydroxybenzoate
UDP + 4-(beta-D-glucosyloxy)benzoate
-
Substrates: -
Products: -
Products: -
ir
UDPglucose + 4-hydroxybenzoate
UDP + 4-(beta-D-glucosyloxy)benzoate
-
Substrates: -
Products: -
Products: -
ir
additional information
?
-
-
Substrates: key step in the shikonin biosynthesis
Products: -
Products: -
?
additional information
?
-
-
Substrates: enhanced shikonin formation induced by brassinoide involves regulation of this key enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: probably control of activity during pine pollen germination
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
Substrates: key step in the shikonin biosynthesis
Products: -
Products: -
?
additional information
?
-
-
Substrates: enhanced shikonin formation induced by brassinoide involves regulation of this key enzyme
Products: -
Products: -
?
additional information
?
-
-
Substrates: probably control of activity during pine pollen germination
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4-(beta-D-glucosyloxy)benzoate
-
non competitive inhibition with respect to both 4-hidroxybenzoic acid and UDP
UDP
-
non competitive inhibition with respect to 4-hydroxybenzoic acid, competitive inhibitor with respect to UDPglucose
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
EDTA
-
60% increase of activity at 0.1 mM, probably due to removal of heavy metal ions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.002196
-
the activity of p-hydroxylbenzoic acid glucosyltransferase decreases from 0.002586 micromol/min/mg to 0.002196 micromol/min/mg at 16 Gy of gamma radiation. Samples are taken two weeks after radiation.
0.002292
-
the activity of p-hydroxylbenzoic acid glucosyltransferase decreases from 0.002586 micromol/min/mg to 0.002292 micromol/min/mg at 2 Gy of gamma radiation. Samples are taken two weeks after radiation.
0.002586
-
the activity of p-hydroxylbenzoic acid glucosyltransferase decreases from 0.002586 micromol/min/mg to 0.002292 micromol/min/mg at 2 Gy of gamma radiation. Samples are taken two weeks after radiation.
additional information
-
activity is regulated by white light in cell suspension cultures
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
the used cell line is induced from the root of Lithospermum erythrorhizon plants, cell line 622-46. A 4fold enhancement of shikonin (secondary metabolite) production is observable after a gamma radiation at the callus at 16 Gy. When the activity of p-hydroxybenzoic acid geranyltransferase is high with low activity of p-hydroxylbenzoic acid glucosyltransferase, the process of shikonin biosynthesis is boosted.
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Highest Expressing Human Cell Lines
Cell Line LinksPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0ABD3J6P8_EUCGL
487
0
53978
TrEMBL
Secretory Pathway (Reliability: 2)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.05 M Tris-HCl, 1 mM DTT, 0.02 mM phenylmethylsulfonyl fluoride, pH 7.6, 3 months, 10% loss of activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial, using ammonium sulfate precipitation and chromatography on DEAE-Sepharose and phenyl-Sepharose
-
using gel filtration followed by hydroxyapatite and HiTrap blue affinity chromatography
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bechthold, A.; Berger, U.; Heide, L.
Partial purification, properties, and kinetic studies of UDP-glucose:p-hydroxybenzoate glucosyltransferase from cell cultures of Lithospermum erythrorhizon
Arch. Biochem. Biophys.
288
39-47
1991
Lithospermum erythrorhizon
brenda
Katsumata, T.; Shige, H.; Ejiri, S.
Biochemical studies on pollen. Part XXXIV. UDP-glucose:4-(beta-D-glucopyranosyloxy)benzoic acid glucosyltransferase from the pollen of Pinus densiflora
Phytochemistry
28
359-362
1989
Pinus densiflora
-
brenda
Yazaki, K.; Inushima, K.; Kataoka, M.; Tabata, M.
Intracellular localization of UDPG:p-hydroxybenzoate glucosyltransferase and its reaction product in Lithospermum cell cultures
Phytochemistry
38
1127-1130
1995
Lithospermum erythrorhizon
-
brenda
Gaisser, S.; Heide, L.
Inhibition and regulation of shikonin biosynthesis in suspension cultures of Lithospermum
Phytochemistry
41
1065-1072
1996
Lithospermum erythrorhizon
-
brenda
Li, S.M.; Wang, Z.X.; Heide, L.
Purification of UDP-glucose:4-hydroxybenzoate glucosyltransferase from cell cultures of Lithospermum erythrorhizon
Phytochemistry
46
27-32
1997
Lithospermum erythrorhizon
-
brenda
Yang, Y.H.; Huang, J.; Ding, J.
Interaction between exogenous brassinolide, IAA and BAP in secondary metabolism of cultured Onosma paniculatum cells
Plant Growth Regul.
39
253-261
2003
Onosma paniculata
-
brenda
Chung, B.Y.; Lee, Y.; Baek, M.; Kim, J.; Wi, S.G.; Kim, J.
Effects of low-dose gamma-irradiation on production of shikonin derivatives in callus cultures of Lithospermum erythrorhizon S
Radiat. Phys. Chem.
75
1018-1023
2006
Lithospermum erythrorhizon
-
brenda
EXTERNAL LINKS (specific for EC-Number 2.4.1.194)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
