A configuration-inverting rhamnosyltransferase that converts flavonol 3-O-glucosides to 3-O-rutinosides. Also acts, more slowly, on rutin, quercetin 3-O-galactoside and flavonol 3-O-rhamnosides.
The enzyme appears in viruses and cellular organisms
A configuration-inverting rhamnosyltransferase that converts flavonol 3-O-glucosides to 3-O-rutinosides. Also acts, more slowly, on rutin, quercetin 3-O-galactoside and flavonol 3-O-rhamnosides.
Substrates: the recombinant enzyme is able to use 3-O-glucosylated flavonols but not flavonoid aglycones or 7-O-glycosylated flavonoids as substrates Products: -
Substrates: acceptor substrates are 3-O-glucosides of quercetin, isorhamnetin or kaempferol, rutin, less efficient acceptors: quercetin 3-O-galactoside or quercetin 3-O-rhamnoside, kaempferol 3-O-rhamnoside or myrecetin 3-O-rhamnoside, no substrates: aglycones Products: i.e. 3-O-rutinosides
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcript level is 15.9fold highest at 100 mg/l salicylic acid treatment than that for the none-treated plants. The expression increases to 9.66 times of control plants at 50 mg/l salicylic acid, respectively, and further increases to 15.90 times of the untreated control at 100 mg/l salicylic acid treatment, and then significantly decreases at 150 mg/l salicylic acid, while still maintains 5.13fold higher level than the control. The expression level of the flavonol-3-O-glucoside L-rhamnosyltransferase gene is 1.33fold higher than control plants when treated with 0.010 mM methyl jasmonate and significantly increases to 3.54times of controls at 0.1 mM methyl jasmonate treatment
Kleinehollenhorst, G.; Behrens, H.; Pegels, G.; Strunk, N.; Wiermann, R.
Formation of flavonol 3-O-diglycosides and flavonol 3-O-triglycosides by enzyme extracts from anthers of Tulipa cv apeldoorn - characterization and activity of 3 different O-glycosyltransferases during anther development
Kianersi, F.; Abdollahi, M.R.; Mirzaie-Asl, A.; Dastan, D.; Rasheed, F.
Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate