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IUBMB Comments Cholesterol, sitosterol, campesterol and diacylglycerol can act as acceptors. Transfers a number of long-chain fatty acyl groups.
Synonyms
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1,2-diacyl-sn-glycerol:sterol acyl transferase
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a 1,2-diacyl-sn-glycerol + sterol = a 1-acyl-sn-glycerol + sterol ester
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Acyl group transfer
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1,2-diacyl-sn-glycerol:sterol O-acyltransferase
Cholesterol, sitosterol, campesterol and diacylglycerol can act as acceptors. Transfers a number of long-chain fatty acyl groups.
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1,2-diacyl-sn-glycerol + sterol
monoacylglycerol + sterol ester
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Substrates: -
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campesterol + 1,2-diacyl-sn-glycerol
monoacylglycerol + campesterol-3-O-acyl ester
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Substrates: -
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cholesterol + 1,2-diacyl-sn-glycerol
monoacylglycerol + cholesterol-3-O-acyl ester
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Substrates: -
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diacylglycerol + 1,2-diacyl-sn-glycerol
monoacylglycerol + triacyl-sn-glycerol
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Substrates: -
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dipalmitoylglycerol + sterol
monopalmitoylglycerol + steryl palmitate
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Substrates: -
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sitosterol + 1,2-diacyl-sn-glycerol
monoacylglycerol + sitosterol-3-O-acyl ester
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Substrates: -
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additional information
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additional information
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Substrates: all reactions observed in a mixed micelle system are transacetylation reactions involving various acceptors, examination of pathways of metabolism of diacylglycerol
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additional information
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Substrates: testing of diacylglycerols with only one type of fatty acid, preference for fatty acid transfer
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additional information
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Substrates: monoacylglycerol and triacylglycerol are not suitable substrates
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Digitonin
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effective inhibitor
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bovine serum albumin
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stimulates
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phosphatidylcholine
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indispensable for activity
additional information
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it is possible that Triton X-100 inhibits diacylglycerol degradation and that bovine serum albumin prevents this enzymic inhibition by binding the nonmicellar Triton X-100
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0.0014
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20000 * g pellet from differential centrifugation of the leaf homogenate
0.0022
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88000 * g pellet from differential centrifugation of the leaf homogenate
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brenda
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brenda
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additional information
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differential centrifugation of the leaf homogenate shows the 20000 * g pellet to have the highest amount of enzyme of the fractions tested but the highest specific activity is in the 88000 * g pellet. The active fractions contain endoplasmic reticulum, ribosomes and other membrane vesicles
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Highest Expressing Human Cell Lines
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-15°C, in a desiccator, several weeks
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Garcia, R.E.; Mudd, J.B.
1,2-Diacyl-sn-glycerol: sterol acyl transferase from spinach leaves (Spinacia olerecea L.)
Methods Enzymol.
71
768-772
1981
Spinacia oleracea
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Garcia, R.E.; Mudd, J.B.
Metabolism of monoacylglycerol and diacylglycerol by enzyme preparations from spinach leaves
Arch. Biochem. Biophys.
191
487-493
1978
Spinacia oleracea
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