BRENDA home
History
Information on EC 2.1.1.276 - gibberellin A4 carboxyl methyltransferase
for references in articles please use BRENDA:EC2.1.1.276Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.1.1.276 gibberellin A4 carboxyl methyltransferaseIUBMB Comments
The enzyme also methylates gibberellins A34 (80%), A9 (60%), and A3 (45%) with significant activity.
Specify your search results
The expected taxonomic range for this enzyme is: Arabidopsis thaliana
Reaction Schemes
Synonyms
GAMT2, gibberellin A4 O-methyltransferase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
GAMT2
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + gibberellin A4 = S-adenosyl-L-homocysteine + methyl gibberellin A4
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:gibberellin A4 O-methyltransferase
The enzyme also methylates gibberellins A34 (80%), A9 (60%), and A3 (45%) with significant activity.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + gibberellin A1
S-adenosyl-L-homocysteine + methyl gibberellin A1
30% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A20
S-adenosyl-L-homocysteine + methyl gibberellin A20
15% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A3
O-methyl gibberellin A3 + S-adenosyl-L-homocysteine
S-adenosyl-L-methionine + gibberellin A3
S-adenosyl-L-homocysteine + methyl gibberellin A3
45% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A34
S-adenosyl-L-homocysteine + methyl gibberellin A34
80% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A4
S-adenosyl-L-homocysteine + methyl gibberellin A4
-
-
-
?
S-adenosyl-L-methionine + gibberellin A51
S-adenosyl-L-homocysteine + methyl gibberellin A51
16% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A9
S-adenosyl-L-homocysteine + methyl gibberellin A9
60% of the activity compared to methylation of gibberellin A4
-
-
?
S-adenosyl-L-methionine + gibberellin A3
O-methyl gibberellin A3 + S-adenosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-methionine + gibberellin A3
O-methyl gibberellin A3 + S-adenosyl-L-homocysteine
-
-
-
-
?
additional information
?
-
no activity with gibberelin A12 and gibberelin A19
-
-
?
additional information
?
-
-
no activity with gibberelin A12 and gibberelin A19
-
-
?
additional information
?
-
-
the enzyme does not methylate abscisic acid
-
-
?
additional information
?
-
-
the enzyme does not methylate abscisic acid
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + gibberellin A3
O-methyl gibberellin A3 + S-adenosyl-L-homocysteine
S-adenosyl-L-methionine + gibberellin A4
S-adenosyl-L-homocysteine + methyl gibberellin A4
-
-
-
?
S-adenosyl-L-methionine + gibberellin A3
O-methyl gibberellin A3 + S-adenosyl-L-homocysteine
-
-
-
-
?
S-adenosyl-L-methionine + gibberellin A3
O-methyl gibberellin A3 + S-adenosyl-L-homocysteine
-
-
-
-
?
additional information
?
-
-
the enzyme does not methylate abscisic acid
-
-
?
additional information
?
-
-
the enzyme does not methylate abscisic acid
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+
additional information
-
not enhanced nor inhibited by K+, NH4+, Na+, Ca2+, Fe2þ, Mg2+, and Mn2+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
malfunction
GAMT2 null mutant shows reduced inhibition of germination, compared with the wild type, when placed on plates containing the gibberellin biosynthesis inhibitor ancymidol
malfunction
-
a GAMT2null mutant displays ancymidol- and abscisic acid-resistant phenotype in seed germination assay
malfunction
-
a GAMT2null mutant displays ancymidol- and abscisic acid-resistant phenotype in seed germination assay
-
physiological function
methylation of gibberellins by the enzyme serves to deactivate gibberellins and initiate their degradation as the seeds mature
physiological function
-
the enzyme is involved in seed maturation, germination and silique development by modulating gibberellic acid activity. Enzyme overexpression in Arabidopsis results in multiple phenotypes, including dwarfism, retarded growth, late flowering, and reduced fertility
physiological function
-
the enzyme is involved in seed maturation, germination and silique development by modulating gibberellic acid activity. Enzyme overexpression in Arabidopsis results in multiple phenotypes, including dwarfism, retarded growth, late flowering, and reduced fertility
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GAMT2_ARATH
387
0
43349
Swiss-Prot
other Location (Reliability: 2)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli, expression in Arabidopsis thaliana under the control of the 35S cauliflower mosaic virus promoter
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
during seed germination, the expression of the enzyme decreases until the cotyledons expand out of the seed coat
expressed mainly in whole siliques, peak transcript levels from the middle until the end of silique development
during seed germination, the expression of the enzyme decreases until the cotyledons expand out of the seed coat
-
during seed germination, the expression of the enzyme decreases until the cotyledons expand out of the seed coat
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Varbanova, M.; Yamaguchi, S.; Yang, Y.; McKelvey, K.; Hanada, A.; Borochov, R.; Yu, F.; Jikumaru, Y.; Ross, J.; Cortes, D.; Ma, C.J.; Noel, J.P.; Mander, L.; Shulaev, V.; Kamiya, Y.; Rodermel, S.; Weiss, D.; Pichersky, E.
Methylation of gibberellins by Arabidopsis GAMT1 and GAMT2
Plant Cell
19
32-45
2007
Arabidopsis thaliana (Q5XF78), Arabidopsis thaliana
brenda
Xing, S.; Qin, G.; Shi, Y.; Ma, Z.; Chen, Z.; Gu, H.; Qu, L.
GAMT2 encodes a methyltransferase of gibberellic acid that is involved in seed maturation and germination in Arabidopsis
J. Integr. Plant Biol.
49
368-381
2007
Arabidopsis thaliana, Arabidopsis thaliana ecotype Columbia-0
-
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 2.1.1.276)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
About BRENDA
Help
Download
Contribution
member of
curated at
curated at
Server: brenda1 Ver: 9edf756-main (2022-05-17 10:30:03 +0200)