Information on EC 1.5.4.1 - pyrimidodiazepine synthase

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The expected taxonomic range for this enzyme is: Drosophila melanogaster

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EC NUMBER
COMMENTARY hide
1.5.4.1
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RECOMMENDED NAME
GeneOntology No.
pyrimidodiazepine synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
drosopterin and aurodrosopterin biosynthesis
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Glutathione metabolism
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Folate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
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CAS REGISTRY NUMBER
COMMENTARY hide
93586-06-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-pyruvoyl-tetrahydropterin + glutathione
pyrimidodiazepine + glutathione disulfide
show the reaction diagram
6-pyruvoyltetrahydropterin + glutathione
glutathione disulfide + pyrimidodiazepine
show the reaction diagram
dihydroneopterin triphosphate + glutathione
?
show the reaction diagram
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-
-
-
-
glutathione disulfide + pyrimidodiazepine
6-pyruvoyltetrahydropterin + glutathione
show the reaction diagram
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-
-
-
r
pyruvoyltetrahydropterin + glutathione
2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido[4,5-b]-[1,4]diazepine
show the reaction diagram
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-
PDA
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
6-pyruvoyl-tetrahydropterin + glutathione
pyrimidodiazepine + glutathione disulfide
show the reaction diagram
Q9VMQ9
essential step in the biosynthesis of red components of the flye's eye pigments drosopterins
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-
?
6-pyruvoyltetrahydropterin + glutathione
glutathione disulfide + pyrimidodiazepine
show the reaction diagram
-
enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase
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r
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
required for the in vitro assay
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0085
dihydroneopterin triphosphate
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1.8
glutathione
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
124
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30°C, pH 7.5
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
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2 * 24000, SDS-PAGE
28000
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2 * 28000, SDS-PAGE
48000
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gel filtration
56000
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gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, enzyme purified through the Affi-Gel blue step is stable for at least 9 months
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-80°C, enzyme purified through the final step stable for at least 1-2 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
gene encoding enzyme is the structural gene for the eye colour mutant sepia
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LINKS TO OTHER DATABASES (specific for EC-Number 1.5.4.1)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)