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IUBMB Comments In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
The expected taxonomic range for this enzyme is: Drosophila melanogaster
Synonyms
More, PDA synthase,
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additional information
enzyme belongs to the thioredoxin-like protein superfamily
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2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione
2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione
in the reverse direction of reaction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine involved is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
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2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyltetrahydropterin + 2 glutathione
active site contains the sequence Cys-Pro-Tyr-Cys
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2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one:glutathione-disulfide oxidoreductase (ring-opening, cyclizing)
In the reverse direction, the reduction of 6-pyruvoyl-tetrahydropterin is accompanied by the opening of the 6-membered pyrazine ring and the formation of the 7-membered diazepine ring. The pyrimidodiazepine formed is an acetyldihydro derivative. Involved in the formation of the eye pigment drosopterin in Drosophila melanogaster.
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6-pyruvoyl-tetrahydropterin + glutathione
pyrimidodiazepine + glutathione disulfide
6-pyruvoyltetrahydropterin + glutathione
glutathione disulfide + pyrimidodiazepine
dihydroneopterin triphosphate + glutathione
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glutathione disulfide + pyrimidodiazepine
6-pyruvoyltetrahydropterin + glutathione
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pyruvoyltetrahydropterin + glutathione
2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido[4,5-b]-[1,4]diazepine
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PDA
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6-pyruvoyl-tetrahydropterin + glutathione
pyrimidodiazepine + glutathione disulfide
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6-pyruvoyl-tetrahydropterin + glutathione
pyrimidodiazepine + glutathione disulfide
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6-pyruvoyl-tetrahydropterin + glutathione
pyrimidodiazepine + glutathione disulfide
essential step in the biosynthesis of red components of the flye's eye pigments drosopterins
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6-pyruvoyltetrahydropterin + glutathione
glutathione disulfide + pyrimidodiazepine
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6-pyruvoyltetrahydropterin + glutathione
glutathione disulfide + pyrimidodiazepine
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enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase
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6-pyruvoyl-tetrahydropterin + glutathione
pyrimidodiazepine + glutathione disulfide
essential step in the biosynthesis of red components of the flye's eye pigments drosopterins
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6-pyruvoyltetrahydropterin + glutathione
glutathione disulfide + pyrimidodiazepine
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enzyme is involved in the formation of the eye pigment drosopterin in Drosophila melanogaster, eye color mutant sepia is missing PDA synthase
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r
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2-mercaptoethanol
required for the in vitro assay
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0.0085
dihydroneopterin triphosphate
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enzyme mRNA is present at late pupal stage at a high level and in early adult, but in substantially lower level
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brenda
gene clot
SwissProt
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wild-type flies
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brenda
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exclusive expression in the head
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SEPIA_DROME
243
0
28413
Swiss-Prot
other Location (Reliability: 4 )
Q9VMQ9_DROME
126
0
14661
TrEMBL
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24000
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2 * 24000, SDS-PAGE
28000
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2 * 28000, SDS-PAGE
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dimer
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2 * 28000, SDS-PAGE
dimer
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2 * 24000, SDS-PAGE
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-80°C, enzyme purified through the Affi-Gel blue step is stable for at least 9 months
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-80°C, enzyme purified through the final step stable for at least 1-2 weeks
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DNA and amino acid sequence determination and analysis
gene encoding enzyme is the structural gene for the eye colour mutant sepia
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Wiederrecht, G.J.; Brown, G.M.
Purification and properties of the enzymes from Drosophila melanogaster that catalyze the conversion of dihydroneopterin triphosphate to the pyrimidodiazepine precursor of the drosopterins
J. Biol. Chem.
259
14121-14127
1984
Drosophila melanogaster
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Giordano, E.; Peluso, I.; Rendina, R.; Digilio, A.; Furia, M.
The clot gene of Drosophila melanogaster encodes a conserved member of the thioredoxin-like protein superfamily
Mol. Genet. Genomics
268
692-697
2003
Drosophila melanogaster (Q9VMQ9)
brenda
Kim, J.; Suh, H.; Kim, S.; Kim, K.; Ahn, C.; Yim, J.
Identification and characteristics of the structural gene for the Drosophila eye colour mutant sepia, encoding PDA synthase, a member of the Omega class glutathione S-transferases
Biochem. J.
398
451-460
2006
Drosophila melanogaster
brenda
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1.5.4.1 pyrimidodiazepine synthase
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