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IUBMB CommentsThe enzyme cleaves the anthraquinone ring of questin to form a benzophenone. Involved in the biosynthesis of the seco-anthraquinone (+)-geodin.
The expected taxonomic range for this enzyme is: Aspergillus terreus
Synonyms
questin oxygenase,
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questin + NADPH + H+ + O2 = demethylsulochrin + NADP+
The enzyme cleaves the anthraquinone ring of questin to form a benzophenone. Involved in the biosynthesis of the seco-anthraquinone (+)-geodin
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questin,NADPH:oxygen oxidoreductase (hydroxylating, anthraquinone-ring-opening)
The enzyme cleaves the anthraquinone ring of questin to form a benzophenone. Involved in the biosynthesis of the seco-anthraquinone (+)-geodin.
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questin + NADPH + H+ + O2
demethylsulochrin + NADP+
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absolute requirement for oxygen and NADPH
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questin + NADPH + H+ + O2
demethylsulochrin + NADP+
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absolute requirement for oxygen and NADPH
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brenda
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brenda
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brenda
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complete loss of activity overnight
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very unstable, polyols, non-ionic detergents and EDTA have stabilizing effects
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cold room, 0.01 M Tris-HCl, pH 7.5, 50% ethylene glycol, v/v, 1 mM EDTA, 0.05% Tween 80, w/v, 70-80% of its original activity after 2 days
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partial, additional functional protein or proteins required for full activity
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Fuji, I.; Ebizuka, Y.; Sankawa, U.
A novel anthraquinone ring cleavage enzyme from Aspergillus terreus
J. Biochem.
103
878-883
1988
Aspergillus terreus
brenda
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1.14.13.43 questin monooxygenase
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