Information on EC 1.14.13.43 - questin monooxygenase

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The expected taxonomic range for this enzyme is: Aspergillus terreus

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EC NUMBER
COMMENTARY hide
1.14.13.43
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RECOMMENDED NAME
GeneOntology No.
questin monooxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
questin + NADPH + H+ + O2 = demethylsulochrin + NADP+
show the reaction diagram
The enzyme cleaves the anthraquinone ring of questin to form a benzophenone. Involved in the biosynthesis of the seco-anthraquinone (+)-geodin
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
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reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
geodin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
questin,NADPH:oxygen oxidoreductase (hydroxylating, anthraquinone-ring-opening)
The enzyme cleaves the anthraquinone ring of questin to form a benzophenone. Involved in the biosynthesis of the seco-anthraquinone (+)-geodin.
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CAS REGISTRY NUMBER
COMMENTARY hide
115232-45-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
questin + NADPH + H+ + O2
demethylsulochrin + NADP+
show the reaction diagram
-
absolute requirement for oxygen and NADPH
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
questin + NADPH + H+ + O2
demethylsulochrin + NADP+
show the reaction diagram
-
absolute requirement for oxygen and NADPH
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
-
highly specific
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.03
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crude extract
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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complete loss of activity overnight
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very unstable, polyols, non-ionic detergents and EDTA have stabilizing effects
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
cold room, 0.01 M Tris-HCl, pH 7.5, 50% ethylene glycol, v/v, 1 mM EDTA, 0.05% Tween 80, w/v, 70-80% of its original activity after 2 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, additional functional protein or proteins required for full activity
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.43)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)