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EC Tree
IUBMB Comments Also oxidizes, more slowly, the cis isomer and 2-hydroxycyclohexanone.
The expected taxonomic range for this enzyme is: Acinetobacter sp.
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trans-cyclohexane-1,2-diol + NAD+ = 2-hydroxycyclohexan-1-one + NADH + H+
Also oxidizes, more slowly, the cis-isomer and 2-hydroxy-cyclohexanone
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trans-cyclohexane-1,2-diol:NAD+ 1-oxidoreductase
Also oxidizes, more slowly, the cis isomer and 2-hydroxycyclohexanone.
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trans-cyclohexane-1,2-diol + NAD+
2-hydroxycyclohexan-1-one + NADH
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metabolic pathway of trans-cyclohexan-1,2-diol to adipate
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r
trans-cyclohexane-1,2-diol + NAD+
2-hydroxycyclohexan-1-one + NADH + H+
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trans-cyclohexane-1,2-diol + NAD+
2-hydroxycyclohexan-1-one + NADH
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metabolic pathway of trans-cyclohexan-1,2-diol to adipate
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trans-cyclohexane-1,2-diol + NAD+
2-hydroxycyclohexan-1-one + NADH + H+
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NAD+
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0.85
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cell extracts, grown on trans-cyclohexan-1,2-diol
26.5
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partially purified
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7
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reduction of 2-hydroxycyclohexan-1-one
9.5 - 10
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oxidation of trans-cyclohexane-1,2-diol
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induction of enzyme formation by trans-cyclohexan-1,2-diol containing medium
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induction of enzyme formation by trans-cyclohexan-1,2-diol containing medium
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induction of enzyme formation by trans-cyclohexan-1,2-diol containing medium
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L181F
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random mutagenesis, A site mutant
L181F/I263N
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random mutagenesis, A site mutant
V78I/A82F/A328F/T438I
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random mutagenesis, B site mutant
V78I/A82I/A328N
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random mutagenesis, B site mutant
additional information
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whole-cell-catalyzed multiple regio- and stereoselective functionalizations in cascade reactions enabled by directed evolution: in Escherichia coli, expression of the recombinant enzyme leads to four different mutant whole cells, each leading to one of the three possible stereoisomeric forms of 1,2-cyclohexanediol, namely (R,R)-5, (S,S)-5, (S,R)-5 (meso), or (R,S)-5 (also meso), in a multi-step sequence, overview. Starting from the achiral substrates cyclohexane, cyclohexanol, or cyclohexanone, cyclohexane-1,2-diols as functionalized products are shown to be accessible in three different stereoisomeric forms
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functional recombinant expression in Escherichia coli
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Davey, J.F.; Trudgill, P.W.
The metabolism of trans-cyclohexan-1,2-diol by an Acinetobacter species
Eur. J. Biochem.
74
115-127
1977
Acinetobacter sp., Acinetobacter sp. TD63
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Li, A.; Ilie, A.; Sun, Z.; Lonsdale, R.; Xu, J.; Reetz, M.
Whole-cell-catalyzed multiple regio- and stereoselective functionalizations in cascade reactions enabled by directed evolution
Angew. Chem. Int. Ed. Engl.
55
12026-12029
2016
Bacillus megaterium
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