Information on EC 3.8.1.8 - atrazine chlorohydrolase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
3.8.1.8
-
RECOMMENDED NAME
GeneOntology No.
atrazine chlorohydrolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
atrazine + H2O = 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
-
-
-
-
atrazine + H2O = 4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
catalytic mechanism, the Glu241 side chain provides a proton to N-1 of the s-triazine substrate to facilitate nucleophilic displacement at the adjacent C-2, overview
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Deamination
-
-
Deamination
-
-
-
dechlorination
-
-
dechlorination
-
-
-
hydrolysis
-
-
hydrolysis
-
-
hydrolysis
-
-
-
hydrolysis of C-halide
-
-
hydrolysis of C-halide
-
;
-
PATHWAY
KEGG Link
MetaCyc Link
Atrazine degradation
-
atrazine degradation I (aerobic)
-
atrazine degradation III
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
atrazine chlorohydrolase
Involved in the degradation of the herbicide atrazine, 2-chloro-4-(ethylamino)-6-(isopropylamino)-1,3,5-triazine, in bacteria.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
atrazine chlorohydrolase
-
-
atrazine chlorohydrolase
-
-
atrazine chlorohydrolase
Escherichia coli JM109
-
-
-
atrazine chlorohydrolase
-
-
atrazine chlorohydrolase 2
-
-
atrazine dechlorinase
-
-
-
-
AtzA
-
-
-
AtzA
Arthrobacter nicotinovorans HIM
-
-
-
AtzA
Q8GG87
-
AtzA
Q8GG87
-
-
AtzA
Clavibacter michiganese
-
-
AtzA
Clavibacter michiganese ATZ1
-
-
-
AtzA
Escherichia coli JM109
-
-
-
AtzA
-
-
AtzA
P72156
-
AtzA
-, P72156
-
-
AtzB
-
-
-
dechlorinase, atrazine (9CI)
-
-
-
-
hydroxyatrazine N-ethylaminohydrolase
-
-
triazine hydrolase
-
-
TrZN
-
-
hydroxyatrazine N-ethylaminohydrolase
-
-
-
additional information
-
enzyme shows 98% amino acid sequence identitiy with melamine deaminase from Pseudomonas sp. strain NRRL B-12227, but does not catalyse the same reaction
additional information
-
enzyme shows 98% amino acid sequence identitiy with melamine deaminase from Pseudomonas sp. strain NRRL B-12227, but does not catalyse the same reaction
-
CAS REGISTRY NUMBER
COMMENTARY
168680-16-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain ST96-4 containing plasmid pADP1::Tn5
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens J14a
strain J14a
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens ST96-4
strain ST96-4 containing plasmid pADP1::Tn5
-
-
Manually annotated by BRENDA team
strain SG1
-
-
Manually annotated by BRENDA team
Alcaligenes sp. SG1
strain SG1
-
-
Manually annotated by BRENDA team
Arthrobacter nicotinovorans HIM
strain HIM
-
-
Manually annotated by BRENDA team
Arthrobacter sp. strain MCM B-436
-
-
Manually annotated by BRENDA team
gene atzA
UniProt
Manually annotated by BRENDA team
gene atzA
UniProt
Manually annotated by BRENDA team
Clavibacter michiganese
-
-
-
Manually annotated by BRENDA team
Clavibacter michiganese
strain ATZ1
-
-
Manually annotated by BRENDA team
Clavibacter michiganese ATZ1
-
-
-
Manually annotated by BRENDA team
Clavibacter michiganese ATZ1
strain ATZ1
-
-
Manually annotated by BRENDA team
Escherichia coli JM109
JM109
-
-
Manually annotated by BRENDA team
strain C190
-
-
Manually annotated by BRENDA team
strain C190
-
-
Manually annotated by BRENDA team
gene atzA
UniProt
Manually annotated by BRENDA team
Pseudomonas sp. starin ADP
-
-
Manually annotated by BRENDA team
strain ADP, gene atzA
-
-
Manually annotated by BRENDA team
gene atzA
UniProt
Manually annotated by BRENDA team
strain ADP, gene atzA
-
-
Manually annotated by BRENDA team
Ralstonia pickettii D
D
-
-
Manually annotated by BRENDA team
strain M91-3
-
-
Manually annotated by BRENDA team
Ralstonia sp. M91-3
strain M91-3
-
-
Manually annotated by BRENDA team
strain PATR
-
-
Manually annotated by BRENDA team
Rhizobium sp. PATR
strain PATR
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
atrazine chlorohydrolase 2 initiates bacterial metabolism of the herbicide atrazine by hydrolytic displacement of a chlorine substituent from the s-triazine ring
evolution
-
TrzN uniquely positions threonine 325 in place of a conserved aspartate that ligates the metal in most mononuclear amidohydrolases superfamily members
additional information
Clavibacter michiganese, Pseudomonas sp.
-
development of fiber-optic biosensors for detection of atrazine using the atrazine chlorohydrolase, quantification of hydrochloric acid release, optimization, overview
additional information
Clavibacter michiganese ATZ1, Pseudomonas sp. ADP
-
development of fiber-optic biosensors for detection of atrazine using the atrazine chlorohydrolase, quantification of hydrochloric acid release, optimization, overview
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-di(N-isobutylamino)-6-hydroxy-1,3,5-triazine + H2O
6-[(2-methylpropyl)amino]-1,3,5-triazine-2,4-diol + isobutylamine
show the reaction diagram
-
-
-
-
?
2,4-di(N-secbutylamino)-6-hydroxy-1,3,5-triazine + H2O
6-[(1-methylpropyl)amino]-1,3,5-triazine-2,4-diol + butan-2-amine
show the reaction diagram
-
-
-
-
?
2,4-di(N-tertbutylamino)-6-hydroxy-1,3,5-triazine + H2O
6-(tert-butylamino)-1,3,5-triazine-2,4-diol + tert-butylamine
show the reaction diagram
-
-
-
-
?
2,4-diamino-6-hydroxy-1,3,5-triazine + H2O
6-amino-1,3,5-triazine-2,4-diol + NH3
show the reaction diagram
-
-
-
-
?
2,4-diethylamino-6-hydroxy-1,3,5-triazine + H2O
6-(methylamino)-1,3,5-triazine-2,4-diol + ethylamine
show the reaction diagram
-
-
-
-
?
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine + H2O
6-[(1-methylethyl)amino]-1,3,5-triazine-2,4-diol + propan-2-amine
show the reaction diagram
-
-
-
-
?
2,4-dimethylamino-6-hydroxy-1,3,5-triazine + H2O
6-(methylamino)-1,3,5-triazine-2,4-diol + methylamine
show the reaction diagram
-
-
-
-
?
2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O
6-[ethyl(methyl)amino]-1,3,5-triazine-2,4-diol + ethylamine
show the reaction diagram
-
-
-
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + ethylamine
show the reaction diagram
-
-
-
-
?
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
6-(ethylamino)-1,3,5-triazine-2,4-diol + propan-2-amine
show the reaction diagram
-
-
-
-
?
2-amino-4-chloro-6-hydroxy-1,3,5-triazine + H2O
6-amino-1,3,5-triazine-2,4-diol + Cl-
show the reaction diagram
-
-
-
-
?
2-amino-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + NH3
show the reaction diagram
-
-
-
-
?
2-amino-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
6-(isopropylamino)-1,3,5-triazine-2,4-diol + NH3
show the reaction diagram
-
-
-
-
?
2-amino-6-(N-ethylamino)-4-hydroxy-1,3,5-triazine + H2O
6-(ethylamino)-1,3,5-triazine-2,4-diol + NH3
show the reaction diagram
-
-
-
-
?
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine + H2O
6-(ethylamino)-1,3,5-triazine-2,4-diol + Cl-
show the reaction diagram
-
-
-
-
?
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
6-(isopropylamino)-1,3,5-triazine-2,4-diol + Cl-
show the reaction diagram
-
-
-
-
?
2-hydroxy-4,6-di(N-hydroxyethylamino)-1,3,5-triazine + H2O
6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + 2-aminoethanol
show the reaction diagram
-
-
-
-
?
2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O
?
show the reaction diagram
-
-
-
-
?
6-(ethylamino)-4-(methylamino)-1,3,5-triazin-2-ol + H2O
6-(ethylamino)-1,3,5-triazine-2,4-diol + methylamine
show the reaction diagram
-
-
-
-
?
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O
6-[(1-methylethyl)amino]-1,3,5-triazine-2,4-diol + methylamine
show the reaction diagram
-
-
-
-
?
ametryn + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + H-S-CH3
show the reaction diagram
-
i.e. 2-thiomethyl-4-isopropylamino-6-ethylamino-1,3,5-triazine, a s-triazine herbicide
-
-
?
atratone + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + methanol
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Clavibacter michiganese
-
utilization of atrazines with Cl exchanged for F, CN, N3, OCH3, SCH3
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
catabolic atrazine degradation pathway, overview, dechlorination
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
specific for halo-substituted triazine ring compounds, dehalogenation and degradation of the herbicide atrazine, specific for halo-substituted triazine ring compounds
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine, herbicide
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
specific for halo-substituted triazine ring compounds, dehalogenation and degradation of the herbicide atrazine, specific for halo-substituted triazine ring compounds
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-, catabolic atrazine degradation pathway, overview, dechlorination
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Agrobacterium tumefaciens J14a
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Agrobacterium tumefaciens J14a
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Ralstonia sp. M91-3
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Escherichia coli JM109
-
6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine, herbicide
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Alcaligenes sp. SG1
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Alcaligenes sp. SG1, Ralstonia pickettii D, Rhizobium sp. PATR
-
overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Rhizobium sp. PATR
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Clavibacter michiganese ATZ1
-
utilization of atrazines with Cl exchanged for F, CN, N3, OCH3, SCH3, overview, diverse atrazine derivates, i.e. chlorodialkylamino triazines, tested as substrates
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
Pseudomonas sp., Clavibacter michiganese
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
P72156
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
Q8GG87
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
-
i.e. 2-chloro-4-isopropylamino-6-ethylamino-1,3,5-triazine, a s-triazine herbicide
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
P72156
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
Q8GG87
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + chloride
show the reaction diagram
Clavibacter michiganese ATZ1
-
-
-
-
?
atrazine with Cl to F exchange + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HF
show the reaction diagram
Pseudomonas sp., Alcaligenes sp., Rhizobium sp., Agrobacterium tumefaciens, Ralstonia pickettii, Pseudomonas sp. ADP, Agrobacterium tumefaciens J14a, Alcaligenes sp. SG1, Ralstonia pickettii D, Rhizobium sp. PATR
-
-
-
-
?
cyanazine + H2O
2-{[4-(ethylamino)-6-hydroxy-1,3,5-triazin-2-yl]amino}-2-methylpropanenitrile + HCl
show the reaction diagram
Arthrobacter nicotinovorans, Arthrobacter nicotinovorans HIM
-
-
-
-
?
desethylatrazine + H2O
2-hydroxy-4-amino-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
prometryn + H2O
?
show the reaction diagram
Arthrobacter nicotinovorans, Arthrobacter nicotinovorans HIM
-
-
-
-
?
propazine + H2O
4,6-bis(propan-2-ylamino)-1,3,5-triazin-2-ol + HCl
show the reaction diagram
Arthrobacter nicotinovorans, Arthrobacter nicotinovorans HIM
-
-
-
-
?
simazine + H2O
4,6-diethylamino-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
simazine + H2O
4,6-diethylamino-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
-
simazine + H2O
4,6-diethylamino-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
simazine + H2O
4,6-diethylamino-1,3,5-triazine + HCl
show the reaction diagram
Arthrobacter nicotinovorans HIM
-
-
-
-
-
terbuthylazine + H2O
?
show the reaction diagram
-
-
-
-
?
atrazine + H2O
cyanuric acid + ?
show the reaction diagram
Arthrobacter nicotinovorans, Arthrobacter nicotinovorans HIM
-
-
-
-
?
atrazine + H2O
additional information
-
-
-
following metabolites are detected, hydroxyatrazine, desisopropyl atrazine, N-isopropylammelide
-
?
hydroxyatrazine + H2O
N-isopropylammelide + ethylamine
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no activity with melamine and ammeline
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
catabolic atrazine degradation pathway, overview, dechlorination
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
specific for halo-substituted triazine ring compounds, dehalogenation and degradation of the herbicide atrazine
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine, herbicide
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
-
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
specific for halo-substituted triazine ring compounds, dehalogenation and degradation of the herbicide atrazine
-
-
?
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
-
catabolic atrazine degradation pathway, overview, dechlorination
-
-
ir
atrazine + H2O
4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl
show the reaction diagram
Escherichia coli JM109
-
6-chloro-N2-ethyl-N4-isopropyl-1,3,5-triazine-2,4-diamine, herbicide
-
-
?
hydroxyatrazine + H2O
N-isopropylammelide + ethylamine
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no activity with melamine and ammeline
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
activates
Fe2+
-
iron containing metalloenzyme, 1:1 metal to subunit stoichiometry, bound at the active site, activates
Zn2+
-
required
Mn2+
-
slightly stimulating
additional information
-
no metal ions detectable with the enzym expressed in E. coli
additional information
-
influence of and reconstitution with metals Mn2+, Fe2+, and Co2+, overview
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
-
complete inhibition, reversible by addition of Fe2+, Mn2+, or Co2+
aminotriazine
-
-
Cu2+
-
complete inhibition, not due to displacement of the native active site metal ion Fe2+
Ni2+
-
complete inhibition, not due to displacement of the native active site metal ion Fe2+
oxalic acid
-
reversible by addition of Fe2+, Mn2+, or Co2+
Zn2+
-
complete inhibition, not due to displacement of the native active site metal ion Fe2+
Fe3+
-
complete inhibition, not due to displacement of the native active site metal ion Fe2+
-
additional information
-
no inhibition by melamine and 2-chloro-4,6-diamino-S-triazine
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.029
-
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine
-
deamination
0.02
-
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine
-
deamination
0.23
-
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine
-
dechlorination
0.12
-
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine
-
dechlorination
0.04
-
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine
-
deamination
3.9
-
ametryn
-
wild-type enzyme, pH 7.0, temperature not specified in the publication
7.8
-
ametryn
-
mutant R325, pH and temperature not specified in the publicationS
0.0238
-
Atrazine
-
-
0.049
-
Atrazine
-
A216A/T217D/T219E/A220A/D250D, site-directed mutagenesis, AtzA-variant 288, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.049
-
Atrazine
-
T217D/T219E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.062
-
Atrazine
-
A216G/T217D/T219G/A220H/D250D, site-directed mutagenesis, AtzA-variant 734, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.062
-
Atrazine
-
A216G/T217D/T219G/A220H/D250D, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.067
-
Atrazine
-
A216S/T217A/T219P/A220F/D250G, site-directed mutagenesis, AtzA-variant 357, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.067
-
Atrazine
-
A216S/T217A/T219P/A220F/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.076
-
Atrazine
-
A216A/T217S/T219H/A220G/D250Y, site-directed mutagenesis, AtzA-variant 431, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.076
-
Atrazine
-
T217S/T219H/A220G/D250Y, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.09
-
Atrazine
-
A216G/T217D/T219G/A220H/D250G, site-directed mutagenesis, AtzA-variant 430, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.092
-
Atrazine
-
A216S/T217D/T219V/A220H/D250G, site-directed mutagenesis, AtzA-variant 297, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme; A216Y/T217D/T219Y/A220H/D250V, site-directed mutagenesis, AtzA-variant 422, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.092
-
Atrazine
-
A216S/T217D/T219V/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm; A216Y/T217D/T219Y/A220H/D250V, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.093
-
Atrazine
-
A216Y/T217D/T219T/A220H/D250E, site-directed mutagenesis, AtzA best variant consensus, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.093
-
Atrazine
-
A216Y/T217D/A220H/D250E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.095
-
Atrazine
-
A216G/T217D/T219A/A220V/D250W, site-directed mutagenesis, AtzA-variant 305, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.095
-
Atrazine
-
A216G/T217D/T219A/A220V/D250W, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.1
-
Atrazine
-
A216H/T217A/T219E/A220S/D250S, site-directed mutagenesis, AtzA-variant 662, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.1
-
Atrazine
-
A216H/T217A/T219E/A220S/D250S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.105
-
Atrazine
-
A216S/T217D/T219G/A220S/D250D, site-directed mutagenesis, AtzA-variant 841, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.105
-
Atrazine
-
A216S/T217D/T219G/A220S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.149
-
Atrazine
-
-
0.153
-
Atrazine
-
more than 0.153 mM. AtzA wild type, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
0.153
-
Atrazine
-
AtzA wild-type, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
0.9
-
Atrazine
-
A216G/T217D/T219G/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
3.1
-
Atrazine
-
mutant E241Q, pH and temperature not specified in the publication
13.3
-
Atrazine
-
mutant R325S, pH and temperature not specified in the publication
19
-
Atrazine
-
wild-type enzyme, pH 7.0, temperature not specified in the publication
0.02
-
hydroxyatrazine
-
-
additional information
-
additional information
-
reduction in Km observed in the AtzA variants has allowed the full kinetic profile for the AtzA-catalyzed dechlorination of atrazine to be determined for the first time, revealing the hitherto-unreported substrate cooperativity in AtzA
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.8
-
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine
-
deamination
3.2
-
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine
-
deamination
1.6
-
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine
-
dechlorination
1.9
-
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine
-
dechlorination
1.4
-
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine
-
deamination
0.467
-
ametryn
-
mutant R325S, pH and temperature not specified in the publication
6.04
-
ametryn
-
wild-type enzyme, pH 7.0, temperature not specified in the publication
0.0869
-
Atrazine
-
mutant R325S, pH and temperature not specified in the publication
0.137
-
Atrazine
-
mutant E241Q, pH and temperature not specified in the publication
1.49
-
Atrazine
-
wild-type enzyme, pH 7.0, temperature not specified in the publication
2.2
-
Atrazine
-
more than 0.153 mM. AtzA wild type, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
2.2
-
Atrazine
-
AtzA wild-type, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
2.3
-
Atrazine
-
A216S/T217A/T219P/A220F/D250G, site-directed mutagenesis, AtzA-variant 357, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
2.3
-
Atrazine
-
A216S/T217A/T219P/A220F/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
4.3
-
Atrazine
-
A216A/T217D/T219E/A220A/D250D, site-directed mutagenesis, AtzA-variant 288, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
4.3
-
Atrazine
-
T217D/T219E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
6.2
-
Atrazine
-
A216G/T217D/T219A/A220V/D250W, site-directed mutagenesis, AtzA-variant 305, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
6.2
-
Atrazine
-
A216G/T217D/T219A/A220V/D250W, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
6.5
-
Atrazine
-
A216Y/T217D/T219Y/A220H/D250V, site-directed mutagenesis, AtzA-variant 422, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
6.5
-
Atrazine
-
A216Y/T217D/T219Y/A220H/D250V, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
6.8
-
Atrazine
-
A216S/T217D/T219V/A220H/D250G, site-directed mutagenesis, AtzA-variant 297, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
6.8
-
Atrazine
-
A216S/T217D/T219V/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
7.4
-
Atrazine
-
A216H/T217A/T219E/A220S/D250S, site-directed mutagenesis, AtzA-variant 662, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
7.4
-
Atrazine
-
A216H/T217A/T219E/A220S/D250S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
8
-
Atrazine
-
A216A/T217S/T219H/A220G/D250Y, site-directed mutagenesis, AtzA-variant 431, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
8
-
Atrazine
-
T217S/T219H/A220G/D250Y, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
8.5
-
Atrazine
-
A216S/T217D/T219G/A220S/D250D, site-directed mutagenesis, AtzA-variant 841, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
8.5
-
Atrazine
-
A216S/T217D/T219G/A220S, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
11
-
Atrazine
-
-
12.7
-
Atrazine
-
A216G/T217D/T219G/A220H/D250G, site-directed mutagenesis, AtzA-variant 430, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
12.7
-
Atrazine
-
A216G/T217D/T219G/A220H/D250G, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
15.1
-
Atrazine
-
A216G/T217D/T219G/A220H/D250D, site-directed mutagenesis, AtzA-variant 734, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
15.1
-
Atrazine
-
A216G/T217D/T219G/A220H/D250D, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
27.9
-
Atrazine
-
A216Y/T217D/T219T/A220H/D250E, site-directed mutagenesis, AtzA best variant consensus, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme
27.9
-
Atrazine
-
A216Y/T217D/A220H/D250E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm
3
-
hydroxyatrazine
-
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
60
-
ametryn
-
mutant R325S, pH and temperature not specified in the publication
324878
1530
-
ametryn
-
wild-type enzyme, pH 7.0, temperature not specified in the publication
324878
6.5
-
Atrazine
-
mutant R325S, pH and temperature not specified in the publication
7269
44
-
Atrazine
-
mutant E241Q, pH and temperature not specified in the publication
7269
78
-
Atrazine
-
wild-type enzyme, pH 7.0, temperature not specified in the publication
7269
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0001
-
Clavibacter michiganese
-
crude cell extract
0.0017
-
-
crude cell extract
0.0039
-
-
crude cell extract
0.0045
-
-
crude cell extract
0.0048
-
-
crude cell extract
0.0074
-
-
crude cell extract
0.0162
-
-
partially purified enzyme
0.16
-
-
crude extract
0.33
-
-
prurification step ammonium sulfate precipitation
1.05
-
-
prurification step ceramic hydroxyapatite type I column
1.105
-
-
-
3.7
-
-
about, purified recombinant enzyme
4.5
-
-
purified enzyme
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
10
-
mutant E241Q
6.9
-
-
assay at
7
-
-
activity assay
7
-
-
wild-type enzyme
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
23
-
-
assay at
23
-
-
activity assay
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
additional information
-
inducible by atrazine
Manually annotated by BRENDA team
additional information
-
inducible by atrazine
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Agrobacterium tumefaciens ST96-4
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
52000
-
-
subunit molecular mass, determind by SDS-Page
54000
-
-
determined by SDS-PAGE
105000
-
-
holoenzyme molecular mass, determind by gel-filtration
200000
-
-
about, non-denaturating PAGE
245000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
-
-
homodimer
-
(betaalpha)8 barrel enzyme structure
pentamer
-
4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence
pentamer
-
4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence
-
tetramer
-
4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence
tetramer
-
4 * 50000, SDS-PAGE
tetramer
-
4 or 5 * 60000-52400, SDS-PAGE and calculation from DNA-sequence
-
tetramer
Rhizobium sp. PATR
-
4 * 50000, SDS-PAGE
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
purified recombinant Zn2+-bound wild-type TrzN, and zinc-bound TrzN mutant E241Q complexed with ametryn or atratone, hanging drop method, for the wild-typeenzyme: 21 mg/ml TrzN in 25 mM MOPS, pH 5.5, and 1mM ZnCl2 with precipitant solution containing 25% PEG 3350, 0.1 M Bis-Tris, pH 5.5, 0.2 M ammonium sulfate, and 1 mM ZnCl2, 7-8 days at 25°C. For the mutant enzyme: 6.6 mg/ml TrzN-E241Q in 25 mM MOPS, pH 5.5, and 1 mM ZnCl2 with precipitant containing 30% PEG 4000, 0.1 M sodium citrate, pH 5.6, 0.2 M ammonium acetate, and 1 mM ZnCl2, 2 weeks at 25°C, soaking of crystals for 1 h in cryobuffer composed of its mother liquid, 15% glycerol, and excess of the ametryn or atratone powder, X-ray diffraction structure determination and analysis at 1.40 A, 1.93 A, and 1.64 A resolutions, respectively, modeling
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
-
by ammonium sulfate precipitation and filtration using 100 and 50 kDa cut-off membrane filters
-
recombinant from Escherichia coli
-
using a CHT ceramic hydroxyapatite type I column
-
partially purified
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
-
gene atzA, expression in Nicotiana tabacum via Agrobacterium tumefaciens strain LBA4404-mediated transformation and expression under control of the CaMV 35S promoter, the transgenic tobacco lines expressing the wild-type atzA from Arthrobacter sp. strain AD1 show resistance to and a strong ability to degrade atrazine
Q8GG87
-
Clavibacter michiganese
-
expression of holoenzyme and subfragment with catalytic activity in Escherichia coli DH5-alpha
-
gene atzA, expression in Nicotiana tabacum via Agrobacterium tumefaciens strain LBA4404-mediated transformation and expression under control of the CaMV 35S promoter, the transgenic tobacco lines expressing the wild-type atzA from Pseudomonas sp. strain ADP show resistance to and a strong ability to degrade atrazine
P72156
gene atzA, overexpression in Escherichia coli DH5alpha
-
using the vectors pACYC184 and pMD4 for expression in Escherichia coli DH5alpha cells
-
five amino acids within the substrate-binding pocket of AtzA are chosen for randomization yields one variant enzyme with 20fold-greater catalytic efficiency than that of wild-type AtzA
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
E241Q
-
site-directed mutagenesis, isosteric substitution of the active site glutamate, the mutant shows a large diminution in activity with ametryn, no detectable activity with atratone, and a 10fold decrease with atrazine compared to the wild-type TrzN. Activity of the mutant is nearly constant from pH 6.0 to pH 10.0, consistent with the loss of a proton donating group
R325S
-
site-directed mutagenesis, the mutation results in a 12fold decrease in kcat/Km, largely due to kcat
T325D
-
site-directed mutagenesis, inactive active site mutant, altered active site structure compared to the wild-type enzyme
T325E
-
site-directed mutagenesis, inactive active site mutant, altered active site structure compared to the wild-type enzyme
A216A/T217D/T219E/A220A/D250D
-
site-directed mutagenesis, AtzA-variant 288. Km 49 microM, kcat 4.3 s-1, kcat/Km 88000 s-1*M-1
A216A/T217S/T219H/A220G/D250Y
-
site-directed mutagenesis, AtzA-variant 431. Km 76 microM, kcat 8.0 s-1, kcat/Km 110000 s-1*M-1
A216G/T217D/T219A/A220V/D250W
-
site-directed mutagenesis, AtzA-variant 305. Km 95 microM, kcat 6.2 s-1, kcat/Km 65000 s-1*M-1
A216G/T217D/T219G/A220H/D250D
-
site-directed mutagenesis, AtzA-variant 734. Km 62 microM, kcat 15.1 s-1, kcat/Km 240000 s-1*M-1
A216G/T217D/T219G/A220H/D250G
-
site-directed mutagenesis, AtzA-variant 430. Km 90 microM, kcat 12.7 s-1, kcat/Km 140000 s-1*M-1
A216H/T217A/T219E/A220S/D250S
-
site-directed mutagenesis, AtzA-variant 662. Km 100 microM, kcat 7.4 s-1, kcat/Km 74000 s-1*M-1
A216S/T217A/T219P/A220F/D250G
-
site-directed mutagenesis, AtzA-variant 357. Km 67 microM, kcat 2.3 s-1, kcat/Km 34000 s-1*M-1
A216S/T217D/T219G/A220S/D250D
-
site-directed mutagenesis, AtzA-variant 841. Km 105 microM, kcat 8.5 s-1, kcat/Km 81000 s-1*M-1
A216S/T217D/T219V/A220H/D250G
-
site-directed mutagenesis, AtzA-variant 297. Km 92 microM, kcat 6.8 s-1, kcat/Km 74000 s-1*M-1
A216A/T217D/T219E/A220A/D250D
Escherichia coli JM109
-
site-directed mutagenesis, AtzA-variant 288. Km 49 microM, kcat 4.3 s-1, kcat/Km 88000 s-1*M-1
-
A216G/T217D/T219A/A220V/D250W
Escherichia coli JM109
-
site-directed mutagenesis, AtzA-variant 305. Km 95 microM, kcat 6.2 s-1, kcat/Km 65000 s-1*M-1
-
A216S/T217A/T219P/A220F/D250G
Escherichia coli JM109
-
site-directed mutagenesis, AtzA-variant 357. Km 67 microM, kcat 2.3 s-1, kcat/Km 34000 s-1*M-1
-
A216G/T217D/T219A/A220V/D250W
-
AtzA mutant
A216G/T217D/T219G/A220H/D250D
-
AtzA mutant
A216G/T217D/T219G/A220H/D250G
-
AtzA mutant
A216H/T217A/T219E/A220S/D250S
-
AtzA mutant
A216S/T217A/T219P/A220F/D250G
-
AtzA mutant
A216S/T217D/T219G/A220S
-
AtzA mutant
A216S/T217D/T219V/A220H/D250G
-
AtzA mutant
A216Y/T217D/A220H/D250E
-
AtzA mutant
A216Y/T217D/T219Y/A220H/D250V
-
AtzA mutant
T217D/T219E
-
AtzA mutant
T217S/T219H/A220G/D250Y
-
AtzA mutant
A216Y/T217D/T219Y/A220H/D250V
-
site-directed mutagenesis, AtzA-variant 422. Km 92 microM, kcat 6.5 s-1, kcat/Km 71000 s-1*M-1
additional information
-
A216Y/T217D/T219T/A220H/D250E, site-directed mutagenesis, AtzA best variant consensus
A216Y/T217D/T219Y/A220H/D250V
Escherichia coli JM109
-
site-directed mutagenesis, AtzA-variant 422. Km 92 microM, kcat 6.5 s-1, kcat/Km 71000 s-1*M-1
-
additional information
Escherichia coli JM109
-
A216Y/T217D/T219T/A220H/D250E, site-directed mutagenesis, AtzA best variant consensus
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
Clavibacter michiganese
-
fiber-optic biosensors for detection of atrazine at low concentrations in soil using the atrazine chlorohydrolase and quantification of hydrochloric acid release, optimization, overview
analysis
Clavibacter michiganese ATZ1
-
fiber-optic biosensors for detection of atrazine at low concentrations in soil using the atrazine chlorohydrolase and quantification of hydrochloric acid release, optimization, overview
-
biotechnology
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bioremediation
biotechnology
Escherichia coli JM109
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bioremediation
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agriculture
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degradation of chemically stable and toxic herbicide atrazine in soil and groundwater to non-toxic hydroxyatrazine
agriculture
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atrazine metabolites, i.e. alkylamines, are toxic in higher concentrations for the bacteria without buffered milieu due to low pH
analysis
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fiber-optic biosensors for detection of atrazine at low concentrations in soil using the atrazine chlorohydrolase and quantification of hydrochloric acid release, optimization, overview
agriculture
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atrazine metabolites, i.e. alkylamines, are toxic in higher concentrations for the bacteria without buffered milieu due to low pH; degradation of chemically stable and toxic herbicide atrazine in soil and groundwater to non-toxic hydroxyatrazine; degradation of chemically stable and toxic herbicide atrazine in soil and groundwater to non-toxic hydroxyatrazine
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analysis
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fiber-optic biosensors for detection of atrazine at low concentrations in soil using the atrazine chlorohydrolase and quantification of hydrochloric acid release, optimization, overview
-
agriculture
-
degradation of chemically stable and toxic herbicide atrazine in soil and groundwater to non-toxic hydroxyatrazine
agriculture
Rhizobium sp. PATR
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degradation of chemically stable and toxic herbicide atrazine in soil and groundwater to non-toxic hydroxyatrazine
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