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Literature summary for 3.8.1.8 extracted from

  • Seffernick, J.L.; Aleem, A.; Osborne, J.P.; Johnson, G.; Sadowsky, M.J.; Wackett, L.P.
    Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination (2007), J. Bacteriol., 189, 6989-6997.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.8.1.8

Cloned(Commentary)

Cloned (Comment) Organism
using the vectors pACYC184 and pMD4 for expression in Escherichia coli DH5alpha cells Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 hydroxyatrazine
-
 Pseudomonas sp.
0.02
-
 2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine deamination Pseudomonas sp.
0.029
-
 2,4-diisopropylamino-6-hydroxy-1,3,5-triazine deamination Pseudomonas sp.
0.04
-
 6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine deamination Pseudomonas sp.
0.12
-
 2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine dechlorination Pseudomonas sp.
0.23
-
 2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine dechlorination Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 subunit molecular mass, determind by SDS-Page Pseudomonas sp.
105000
-
 holoenzyme molecular mass, determind by gel-filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxyatrazine + H2O Pseudomonas sp.
-
 N-isopropylammelide + ethylamine
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 Pseudomonas sp. starin ADP
-

Purification (Commentary)

Purification (Comment) Organism
using a CHT ceramic hydroxyapatite type I column Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.16
-
 crude extract Pseudomonas sp.
0.33
-
 prurification step ammonium sulfate precipitation Pseudomonas sp.
1.05
-
 prurification step ceramic hydroxyapatite type I column Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-di(N-isobutylamino)-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-[(2-methylpropyl)amino]-1,3,5-triazine-2,4-diol + isobutylamine
-
 ?
2,4-di(N-secbutylamino)-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-[(1-methylpropyl)amino]-1,3,5-triazine-2,4-diol + butan-2-amine
-
 ?
2,4-di(N-tertbutylamino)-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-(tert-butylamino)-1,3,5-triazine-2,4-diol + tert-butylamine
-
 ?
2,4-diamino-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-amino-1,3,5-triazine-2,4-diol + NH3
-
 ?
2,4-diethylamino-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-(methylamino)-1,3,5-triazine-2,4-diol + ethylamine
-
 ?
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-[(1-methylethyl)amino]-1,3,5-triazine-2,4-diol + propan-2-amine
-
 ?
2,4-dimethylamino-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-(methylamino)-1,3,5-triazine-2,4-diol + methylamine
-
 ?
2-(N-ethyl-N-methylamino)-4-ethylamino-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-[ethyl(methyl)amino]-1,3,5-triazine-2,4-diol + ethylamine
-
 ?
2-(N-ethylamino)-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + ethylamine
-
 ?
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-(ethylamino)-1,3,5-triazine-2,4-diol + propan-2-amine
-
 ?
2-amino-4-chloro-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-amino-1,3,5-triazine-2,4-diol + Cl-
-
 ?
2-amino-4-hydroxy-6-(N-hydroxyethylamino)-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + NH3
-
 ?
2-amino-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-(isopropylamino)-1,3,5-triazine-2,4-diol + NH3
-
 ?
2-amino-6-(N-ethylamino)-4-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-(ethylamino)-1,3,5-triazine-2,4-diol + NH3
-
 ?
2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-(ethylamino)-1,3,5-triazine-2,4-diol + Cl-
-
 ?
2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-(isopropylamino)-1,3,5-triazine-2,4-diol + Cl-
-
 ?
2-hydroxy-4,6-di(N-hydroxyethylamino)-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-[(2-hydroxyethyl)amino]-1,3,5-triazine-2,4-diol + 2-aminoethanol
-
 ?
2-hydroxy-4-(N-isopropylamino)-6-(N-(3-methoxypropyl)amino)-1,3,5-triazine + H2O
-
 Pseudomonas sp. ?
-
 ?
6-(ethylamino)-4-(methylamino)-1,3,5-triazin-2-ol + H2O
-
 Pseudomonas sp. 6-(ethylamino)-1,3,5-triazine-2,4-diol + methylamine
-
 ?
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine + H2O
-
 Pseudomonas sp. 6-[(1-methylethyl)amino]-1,3,5-triazine-2,4-diol + methylamine
-
 ?
hydroxyatrazine + H2O
-
 Pseudomonas sp. N-isopropylammelide + ethylamine
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Pseudomonas sp.

Synonyms

Synonyms Comment Organism
AtzB
-
 Pseudomonas sp.
hydroxyatrazine N-ethylaminohydrolase
-
 Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 activity assay Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.8
-
 2,4-diisopropylamino-6-hydroxy-1,3,5-triazine deamination Pseudomonas sp.
1.4
-
 6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine deamination Pseudomonas sp.
1.6
-
 2-chloro-4-(N-ethylamino)-6-hydroxy-1,3,5-triazine dechlorination Pseudomonas sp.
1.9
-
 2-chloro-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine dechlorination Pseudomonas sp.
3
-
 hydroxyatrazine
-
 Pseudomonas sp.
3.2
-
 2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine deamination Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 activity assay Pseudomonas sp.