Literature summary for 3.8.1.8 extracted from

Seffernick, J.L.; McTavish, H.; Osborne, J.P.; de Souza, M.L.; Sadowsky, M.J.; Wackett, L.P.
Atrazine chlorohydrolase from Pseudomonas sp. strain ADP is a metalloenzyme (2002), Biochemistry, 41, 14430-14437.

Search for more literature for 3.8.1.8

Cloned(Commentary)

Cloned (Comment)	Organism
gene atzA, overexpression in Escherichia coli DH5alpha	Pseudomonas sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	complete inhibition, reversible by addition of Fe2+, Mn2+, or Co2+	Pseudomonas sp.
Cu2+	complete inhibition, not due to displacement of the native active site metal ion Fe2+	Pseudomonas sp.
Fe3+	complete inhibition, not due to displacement of the native active site metal ion Fe2+	Pseudomonas sp.
Ni2+	complete inhibition, not due to displacement of the native active site metal ion Fe2+	Pseudomonas sp.
oxalic acid	reversible by addition of Fe2+, Mn2+, or Co2+	Pseudomonas sp.
Zn2+	complete inhibition, not due to displacement of the native active site metal ion Fe2+	Pseudomonas sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activates	Pseudomonas sp.
Fe2+	iron containing metalloenzyme, 1:1 metal to subunit stoichiometry, bound at the active site, activates	Pseudomonas sp.
Mn2+	slightly stimulating	Pseudomonas sp.
additional information	influence of and reconstitution with metals Mn2+, Fe2+, and Co2+, overview	Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
atrazine + H2O	Pseudomonas sp.	catabolic atrazine degradation pathway, overview, dechlorination	4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl	-	ir

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	gene atzA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli	Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.7	-	about, purified recombinant enzyme	Pseudomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
atrazine + H2O	-	Pseudomonas sp.	4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl	-	ir
atrazine + H2O	catabolic atrazine degradation pathway, overview, dechlorination	Pseudomonas sp.	4-(ethylamino)-2-hydroxy-6-(isopropylamino)-1,3,5-triazine + HCl	-	ir

Synonyms

Synonyms	Comment	Organism
AtzA	-	Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
23	-	assay at	Pseudomonas sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.9	-	assay at	Pseudomonas sp.

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Release 2023.1 (January 2023)