Reference on EC 3.5.1.93 - glutaryl-7-aminocephalosporanic-acid acylase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kinoshita, T.; Tada, T.; Saito, Y.; Ishii, Y.; Sato, A.; Murata, M.
Crystallization and preliminary X-ray analysis of cephalosporin C acylase from Pseudomonas sp. strain N176
Acta Crystallogr. Sect. D
56
458-459
2000
Pseudomonas sp.
Yamada, H.; Ishii, Y.; Noguchi, Y.; Miura, T.; Mori, T.; Saito, Y.
Protein engineering of a cephalosporin C acylase
Ann. N. Y. Acad. Sci.
799
74-81
1996
Pseudomonas sp.
Binder, R.; Brown, J.; Romancik, G.
Biochemical characterization of a glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain BL072
Appl. Environ. Microbiol.
60
1805-1809
1994
Pseudomonas sp., Pseudomonas sp. BL072
Saito, Y.; Fujimura, T.; Ishii, Y.; Noguchi, Y.; Miura, T.; Niwa, M.; Shimomura, K.
Oxidative modification of a cephalosporin C acylase from Pseudomonas strain N176 and site-directed mutagenesis of the gene
Appl. Environ. Microbiol.
62
2919-2925
1996
Pseudomonas sp.
Zhang, W.; Liu, Y.; Zheng, H.; Yang, S.; Jiang, W.
Improving the activity and stability of GL-7-ACA acylase CA130 by site-directed mutagenesis
Appl. Environ. Microbiol.
71
5290-5296
2005
Pseudomonas sp. (P07662), Pseudomonas sp.
Zhang, Q.J.; Xu, W.X.
Morphological, physiological and enzymatic characteristics of cephalosporin acylase-producing Arthrobacter strain 45-8A
Arch. Microbiol.
159
392-395
1993
Arthrobacter sp., Arthrobacter sp. 45-8A
Oh, B.; Kim, M.; Yoon, J.; Chung, K.; Shin, Y.; Lee, D.; Kim, Y.
Deacylation activity of cephalosporin acylase to cephalosporin C is improved by changing the side-chain conformations of active-site residues
Biochem. Biophys. Res. Commun.
310
19-27
2003
Brevundimonas diminuta
Sio, C.F.; Otten, L.G.; Cool, R.H.; Quax, W.J.
Analysis of a substrate specificity switch residue of cephalosporin acylase
Biochem. Biophys. Res. Commun.
312
755-760
2003
Pseudomonas sp. SY-77-1
Zhang, W.; Huang, X.; Zhao, G.; Jiang, W.
Affinity labeled glutaryl-7-amino cephalosporanic acid acylase C130 can hydrolyze the inhibitor during crystallization
Biochem. Biophys. Res. Commun.
313
555-558
2004
Pseudomonas sp. 130
Park, S.W.; Choi, S.Y.; Chung, K.H.; H43ong, S.I.; Kim, S.W.
Characteristics of GL-7-ACA acylase immobilized on silica gel through silanization
Biochem. Eng. J.
11
87-93
2002
Pseudomonas sp., Pseudomonas sp. KAC-1
-
Kim, J.K.; Yang, I.S.; Rhee, S.; Dauter, Z.; Lee, Y.S.; Park, S.S.; Kim, K.H.
Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation
Biochemistry
42
4084-4093
2003
Pseudomonas sp. (A4ZVL3), Pseudomonas sp.
Ishiye, M.; Niwa, M.
Nucleotide sequence and expression in Escherichia coli of the cephalosporin acylase gene of a Pseudomonas strain
Biochim. Biophys. Acta
1132
233-239
1992
Pseudomonas sp., Pseudomonas sp. V22
Lee, Y.H.; Chang, T.S.; Liu, H.J.; Chu, W.S.
An acidic glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas nitroreducens
Biotechnol. Appl. Biochem.
28
113-118
1998
Pseudomonas nitroreducens, Pseudomonas nitroreducens CCRC 11041
Chen, M.C.; Chen, L.L.; Lee, Y.H.; Wen, C.Y.; Chu, W.S.
Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp
Biotechnol. Tech.
9
859-862
1995
Pseudomonas nitroreducens, Pseudomonas nitroreducens CCRC 11041
-
Kim, Y.; Hol, W.G.
Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity
Chem. Biol.
8
1253-1264
2001
Brevundimonas diminuta (Q9L5D6), Brevundimonas diminuta KAC-1 (Q9L5D6)
Otten, L.G.; Sio, C.F.; van der Sloot, A.M.; Cool, R.H.; Quax, W.J.
Mutational analysis of a key residue in the substrate specificity of a cephalosporin acylase
Chembiochem
5
820-825
2004
Pseudomonas sp. SY-77-1
Ishii, Y.; Saito, Y.; Fujimura, T.; Sasaki, H.; Noguchi, Y.; Yamada, H.; Niwa, M.; Shimomura, K.
High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176
Eur. J. Biochem.
230
773-778
1995
Pseudomonas sp.
Li, Y.; Chen, J.; Jiang, W.; Mao, X.; Zhao, G.; Wang, E.
In vivo post-translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130
Eur. J. Biochem.
262
713-719
1999
Pseudomonas sp. 130 (O86089)
Kim, S.; Kim, Y.
Active site residues of cephalosporin acylase are critical not only for enzymatic catalysis but also for post-translational modification
J. Biol. Chem.
276
48376-48381
2001
Brevundimonas diminuta (Q9L5D6)
Huang, X.; Zeng, R.; Ding, X.; Mao, X.; Ding, Y.; Rao, Z.; Xie, Y.; Jiang, W.; Zhao, G.
Affinity alkylation of the Trp-B4 residue of the beta -subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. 130
J. Biol. Chem.
277
10256-10264
2002
Pseudomonas sp. 130
Otten, L.G.; Sio, C.F.; Vrielink, J.; Cool, R.H.; Quax, W.J.
Altering the substrate specificity of cephalosporin acylase by directed evolution of the beta-subunit
J. Biol. Chem.
277
42121-42127
2002
Pseudomonas sp. (P07662), Pseudomonas sp. SY-77 (P07662)
Lopez-Gallego, F.; Betancor, L.; Hidalgo, A.; Mateo, C.; Guisan, J.M.; Fernandez-Lafuente, R.
Optimization of an industrial biocatalyst of glutaryl acylase: Stabilization of the enzyme by multipoint covalent attachment onto new amino-epoxy Sepabeads
J. Biotechnol.
111
219-227
2004
unidentified
Aramori, I.; Fukagawa, M.; Tsumura, M.; Iwami, M.; Ono, H.; Ishitani, Y.; Koji, H.; Kohsaka, M.; Ueda, Y.; Imanaka, H.
Comparative characterization of new glutaryl 7-ACA and cephalosporin C acylases
J. Ferment. Bioeng.
73
185-192
1992
Brevibacillus laterosporus, Brevundimonas diminuta, Pseudomonas sp., Brevundimonas diminuta N176, Brevibacillus laterosporus J1
-
Biffi, S.; De Martin, L.; Ebert, C.; Gardossi, L.; Linda, P.
Glutaryl-7-ACA acylase catalyses the synthesis of amide bond in heterogeneous substrate mixtures
J. Mol. Catal. B
19-20
135-141
2002
unidentified
-
Kwon, T.H.; Rhee, S.; Lee, Y.S.; Park, S.S.; Kim, K.H.
Crystallization and preliminary X-Ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. GK16
J. Struct. Biol.
131
79-81
2000
Pseudomonas sp.
Li, Y.; Jiang, W.; Yang, Y.; Zhao, G.; Wang, E.
Overproduction and purification of glutaryl 7-amino cephalosporanic acid acylase
Protein Expr. Purif.
12
233-238
1998
Pseudomonas sp. 130
Mao, X.; Wang, W.; Jiang, W.; Zhao, G.P.
His23beta and Glu455beta of the Pseudomonas sp. 130 glutaryl-7-amino cephalosporanic acid acylase are crucially important for efficient autoproteolysis and enzymatic catalysis
Protein J.
23
197-204
2004
Pseudomonas sp. 130
Kim, Y.; Yoon, K.; Khang, Y.; Turley, S.; Hol, W.G.
The 2.0 A crystal structure of cephalosporin acylase
Structure Fold. Des.
8
1059-1068
2000
Brevundimonas diminuta (Q9L5D6), Brevundimonas diminuta
Raimondi, S.; Forti, L.; Monti, D.; Riva, S.
Kinetic resolutions of racemic amines and alcohols catalyzed by an industrial glutaryl-7-aminocephalosporanic acid acylase with unexpected broad substrate specificity
Tetrahedron Asymmetry
14
1091-1094
2003
unidentified
-
Tan, Q.; Song, Q.; Wei, D.
Single-pot conversion of cephalosporin C to 7-aminocephalosporanic acid using cell-bound and support-bound enzymes
Enzyme Microb. Technol.
39
1166-1172
2006
Escherichia coli
-
Yau, M.H.; Wang, J.; Tsang, P.W.; Fong, W.P.
J1 acylase, a glutaryl-7-aminocephalosporanic acid acylase from Bacillus laterosporus J1, is a member of the alpha/beta-hydrolase fold superfamily
FEBS Lett.
580
1465-1471
2006
Brevibacillus laterosporus, Brevibacillus laterosporus J1
Kim, J.K.; Yang, I.S.; Shin, H.J.; Cho, K.J.; Ryu, E.K.; Kim, S.H.; Park, S.S.; Kim, K.H.
Insight into autoproteolytic activation from the structure of cephalosporin acylase: a protein with two proteolytic chemistries
Proc. Natl. Acad. Sci. USA
103
1732-1737
2006
Pseudomonas sp. (A4ZVL3)
Pollegioni, L.; Lorenzi, S.; Rosini, E.; Marcone, G.L.; Molla, G.; Verga, R.; Cabri, W.; Pilone, M.S.
Evolution of an acylase active on cephalosporin C
Protein Sci.
14
3064-3076
2005
Pseudomonas sp.
Lim, J.S.; Park, S.W.; Kim, S.W.
Thermal and operational characteristics of glutaryl-7-aminocephalosporanic acid acylase immobilized on silica gel modified by epoxide silanization
World J. Microbiol. Biotechnol.
22
39-44
2006
Pseudomonas sp., Pseudomonas sp. KAC-1
Khatuntseva, S.A.; Eldarov, M.A.; Lopatin, S.A.; Zeinalov, O.A.; Skryabin, K.G.
Cloning and expression of variants of the glutaryl-7-aminocephalosporic acid acylase of the bacterium Brevundimonas diminuta in Escherichia coli cells
Appl. Biochem. Microbiol.
43
414-421
2007
Brevundimonas diminuta, Brevundimonas diminuta VKM B-1297
-
Sonawane, V.C.
Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes
Crit. Rev. Biotechnol.
26
95-120
2006
Aeromonas sp., Achromobacter xylosoxidans, Arthrobacter sp., Rhizobium viscosum, Brevibacillus laterosporus, Bacillus sp. (in: Bacteria), Brevundimonas diminuta, Burkholderia cepacia, Sphingomonas paucimobilis, Flavobacterium sp., Paecilomyces sp. (in: Eurotiomycetes), Pseudomonas sp., Pseudomonas putida, Pseudomonas nitroreducens, Aeromonas sp. ACY 95, Burkholderia cepacia BY21, Arthrobacter sp. 5-8A, Flavobacterium sp. 650, Pseudomonas sp. A14, Brevibacillus laterosporus J1, Pseudomonas nitroreducens CCRC 11041, Bacillus sp. (in: Bacteria) SV12, Pseudomonas sp. BLO 72, Brevundimonas diminuta N 176
Zhou, H.; Yu, H.; Luo, H.; Shi, Y.; Ma, X.; Shen, Z.
Inducible and constitutive expression of glutaryl-7-aminocephalosporanic acid acylase by fusion to maltose-binding protein
Enzyme Microb. Technol.
40
555-562
2007
Pseudomonas sp.
-
Zheng, H.; Chen, J.; Su, L.; Zhao, Y.; Yang, Y.; Zeng, H.Y.; Xu, G.; Yang, S.; Jiang, W.
One-step purification and immobilization of his-tagged GL-7-ACA acylase
Enzyme Microb. Technol.
41
474-479
2007
Pseudomonas sp. (O86089)
-
Otten, L.G.; Sio, C.F.; Reis, C.R.; Koch, G.; Cool, R.H.; Quax, W.J.
A highly active adipyl-cephalosporin acylase obtained via rational randomization
FEBS J.
274
5600-5610
2007
Pseudomonas sp., Pseudomonas sp. SY-77
Zheng, H.; Zhu, T.; Chen, J.; Zhao, Y.; Jiang, W.; Zhao, G.; Yang, S.; Yang, Y.
Construction of recombinant Escherichia coli D11/pMSTO and its use in enzymatic preparation of 7-aminocephalosporanic acid in one pot
J. Biotechnol.
129
400-405
2007
Pseudomonas sp.
Khatuntseva, S.A.; Eldarov, M.A.; Redo, V.A.; Skryabin, K.G.
Purification and immobilization of recombinant variants of Brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in Escherichia coli cells
J. Biotechnol.
133
123-126
2008
Brevundimonas diminuta
Nupura, H.; Asmita, T.; Sharath, B.; Asmita, P.
Media optimization for the production of cephalosporin C acylase from a novel bacterial source: Alcaligenes xylosoxidans MTCC *491
Res. J. Biotechnol.
3
16-21
2008
Achromobacter xylosoxidans, Achromobacter xylosoxidans MTCC *491
-
Volonte, F.; Marinelli, F.; Gastaldo, L.; Sacchi, S.; Pilone, M.S.; Pollegioni, L.; Molla, G.
Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli
Protein Expr. Purif.
61
131-137
2008
Pseudomonas sp.
Anandan, A.; Vallet, C.; Coyle, T.; Moustafa, I.M.; Vrielink, A.
Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase
Acta Crystallogr. Sect. F
66
808-810
2010
Pseudomonas sp.
Tanomand, A.; Abeshov, R.; Farajnia, S.
Determination of cephalosporin acylase activity by biological and colorimetric method in bacteria
Afr. J. Biotechnol.
8
6697-6699
2009
Pseudomonas sp.
-
Gaurav, K.; Kundu, K.; Kundu, S.
Biosynthesis of cephalosporin-C acylase enzyme: optimal media design, purification, and characterization
Artif. Cells Blood Substit. Immobil. Biotechnol.
38
277-283
2010
Micrococcus luteus
Cho, K.J.; Kim, J.K.; Lee, J.H.; Shin, H.J.; Park, S.S.; Kim, K.H.
Structural features of cephalosporin acylase reveal the basis of autocatalytic activation
Biochem. Biophys. Res. Commun.
390
342-348
2009
Pseudomonas sp. GK16 (A4ZVL3)
Suzuki, H.; Yamada, C.; Kijima, K.; Ishihara, S.; Wada, K.; Fukuyama, K.; Kumagai, H.
Enhancement of glutaryl-7-aminocephalosporanic acid acylase activity of gamma-glutamyltranspeptidase of Bacillus subtilis
Biotechnol. J.
5
829-837
2010
Bacillus subtilis
Zhu, X.; Luo, H.; Chang, Y.; Su, H.; Li, Q.; Yu, H.; Shen, Z.
Characteristic of immobilized cephalosporin C acylase and its application in one-step enzymatic conversion of cephalosporin C to 7-aminocephalosporanic acid
World J. Microbiol. Biotechnol.
27
823-829
2011
Pseudomonas sp., Pseudomonas sp. SE83
Yin, J.; Deng, Z.; Zhao, G.; Huang, X.
The N-terminal nucleophile serine of cephalosporin acylase executes the second autoproteolytic cleavage and acylpeptide hydrolysis
J. Biol. Chem.
286
24476-24486
2011
Pseudomonas sp.
Wang, Y.; Yu, H.; Song, W.; An, M.; Zhang, J.; Luo, H.; Shen, Z.
Overexpression of synthesized cephalosporin C acylase containing mutations in the substrate transport tunnel
J. Biosci. Bioeng.
113
36-41
2012
Pseudomonas sp., Pseudomonas sp. (P15558)
Rosini, E.; Monelli, C.; Pollegioni, L.; Riva, S.; Monti, D.
On the substrate preference of glutaryl acylases
J. Mol. Catal. B
76
52-58
2012
Pseudomonas sp.
-
Luo, H.; Zhao, H.; Chang, Y.; Wang, Q.; Yu, H.; Shen, Z.
Oriented immobilization and characterization of a poly-lysine-tagged cephalosporin C acylase on glyoxyl agarose support
Appl. Biochem. Biotechnol.
175
2114-2123
2014
Pseudomonas sp. (P15557)
Golden, E.; Paterson, R.; Tie, W.; Anandan, A.; Flematti, G.; Molla, G.; Rosini, E.; Pollegioni, L.; Vrielink, A.
Structure of a class III engineered cephalosporin acylase: Comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity
Biochem. J.
451
217-226
2013
Pseudomonas sp.
Conti, G.; Pollegioni, L.; Molla, G.; Rosini, E.
Strategic manipulation of an industrial biocatalyst - evolution of a cephalosporin C acylase
FEBS J.
281
2443-2455
2014
Pseudomonas sp.
Ma, X.; Su, E.; Zhu, Y.; Deng, S.; Wei, D.
High-level expression of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas diminuta NK703 in Escherichia coli by combined optimization strategies
J. Biotechnol.
168
607-615
2013
Brevundimonas diminuta (G3MDB2), Brevundimonas diminuta, Brevundimonas diminuta NK703 (G3MDB2)
Zhang, J.; Yu, H.; Wang, Y.; Luo, H.; Shen, Z.
Determination of the second autoproteolytic cleavage site of cephalosporin C acylase and the effect of deleting its flanking residues in the alpha-C-terminal region
J. Biotechnol.
184
138-145
2014
Pseudomonas sp.
Tian, Y.; Huang, X.; Li, Q.; Zhu, Y.
Computational design of variants for cephalosporin C acylase from Pseudomonas strain N176 with improved stability and activity
Appl. Microbiol. Biotechnol.
101
621-632
2017
Pseudomonas sp. N176 (A0A1D8GRD5)
Jobanputra, A.; Vasait, R.
Cephalosporin C acylase from Pseudomonas species production and enhancement of its activity by optimization of process parameters
Biocatal. Agricult. Biotechnol.
4
465-470
2015
Pseudomonas sp. D13 (A0A1D8GRD5)
-
Wei, Y.; Luo, H.; Chang, Y.; Yu, H.; Shen, Z.
Reversible immobilization of cephalosporin C acylase on epoxy supports coated with polyethyleneimine
Biocatal. Biotransform.
33
250-259
2015
Pseudomonas sp. (A0A1D8GRD5)
-
Luo, H.; Zhu, L.; Chang, Y.; Liu, X.; Liu, Z.; Sun, H.; Li, X.; Yu, H.; Shen, Z.
Microenvironmental pH changes in immobilized cephalosporin C acylase during a proton-producing reaction and regulation by a two-stage catalytic process
Biores. Technol.
223
157-165
2017
Pseudomonas sp. (A0A1D8GRD5)
Ma, X.; Su, E.; Deng, S.; Wei, D.
An effective method for extraction of glutaryl-7-aminocephalosporanic acid acylase from recombinant E. coli cells
Biotechnol. Bioprocess Eng.
20
718-724
2015
Brevundimonas diminuta (G3MDB2), Brevundimonas diminuta NK703 (G3MDB2)
-
Chang, Y.; Tong, S.; Luo, H.; Liu, Z.; Qin, B.; Zhu, L.; Sun, H.; Yu, H.; Shen, Z.
Application of ammonium bicarbonate buffer as a smart microenvironmental pH regulator of immobilized cephalosporin C acylase catalysis in different reactors
Biotechnol. Prog.
35
e2846
2019
Pseudomonas sp. SE83 (P15557)
Conti, G.; Pollegioni, L.; Rosini, E.
One-pot conversion of cephalosporin C by using an optimized two-enzyme process
Catal. Sci. Technol.
5
1854-1863
2015
Pseudomonas sp. N176 (A0A1D8GRD5)
-
Luo, H.; Han, K.; Chang, Y.; Wang, Y.; Tong, S.; Nie, Z.; Wang, X.; Wei, Y.; Yu, H.; Shen, Z.
Effects of inhibitors on the catalysis and immobilization of cephalosporin C acylase
Chem. Biochem. Eng. Q.
32
125-133
2018
Pseudomonas sp. SE83 (P15557)
-
Xu, Z.; Tian, Y.; Zhu, Y.
Computational design of thermostable mutants for cephalosporin C acylase from Pseudomonas strain SE83
Comput. Chem. Eng.
116
112-121
2018
Pseudomonas sp. SE83 (P15557)
-
Isogai, Y.; Nakayama, K.
Alteration of substrate selection of antibiotic acylase from beta-lactam to echinocandin
Protein Eng. Des. Sel.
29
49-56
2015
Pseudomonas sp. SY-77-1 (A4ZVL3)
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