Literature summary for 3.5.1.93 extracted from

Yamada, H.; Ishii, Y.; Noguchi, Y.; Miura, T.; Mori, T.; Saito, Y.
Protein engineering of a cephalosporin C acylase (1996), Ann. N. Y. Acad. Sci., 799, 74-81.

Search for more literature for 3.5.1.93

Application

Application	Comment	Organism
synthesis	immobilized mutant enzyme M269Y/C305S may be a promising enzyme in one-step enzymatic production of 7-aminocephalosporanate from cephalosporin C	Pseudomonas sp.

Protein Variants

Protein Variants	Comment	Organism
C102S	mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C	Pseudomonas sp.
C199S	mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C	Pseudomonas sp.
C277S	mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C	Pseudomonas sp.
C305S	mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C. Expression in Escherichia coli is 2-3fold higher than that of the wild-type enzyme	Pseudomonas sp.
C391S	mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C	Pseudomonas sp.
C493S	mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C	Pseudomonas sp.
C496S	mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C	Pseudomonas sp.
C748S	mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C	Pseudomonas sp.
M269Y/C305S	1.6fold higher activity with cephalosporin C than wild-type enzyme	Pseudomonas sp.

General Stability

General Stability	Organism
immobilized mutant enzyme M269Y/C305S is effective in more than 60 cycles for the production of 7-aminocephalosporanate	Pseudomonas sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
PCMB	-	Pseudomonas sp.

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas sp.	-	N176	-

Storage Stability

Storage Stability	Organism
25°C, 4 weeks, mutant enzyme C305S loses 25% of its activity, mutant enzyme M269Y/C305S loses 25% of its activity	Pseudomonas sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cephalosporin C + H2O	-	Pseudomonas sp.	cephalosporanic acid + 2-amino-5-hydroxypentanoate	-	?
glutaryl-7-aminocephalosporanic acid + H2O	-	Pseudomonas sp.	7-aminocephalosporanate + glutarate	-	?

Synonyms

Synonyms	Comment	Organism
cephalosporin C acylase	-	Pseudomonas sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.102	-	PCMB	-	Pseudomonas sp.

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Release 2023.1 (January 2023)