Information on EC 2.7.1.82 - ethanolamine kinase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.1.82
-
RECOMMENDED NAME
GeneOntology No.
ethanolamine kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + ethanolamine = ADP + O-phosphoethanolamine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phopho group transfer
-
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
choline biosynthesis I
-
-
Glycerophospholipid metabolism
-
-
Metabolic pathways
-
-
phosphatidylethanolamine biosynthesis II
-
-
phosphatidylethanolamine bioynthesis
-
-
plasmalogen biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:ethanolamine O-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9075-78-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NCIB 9205
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
pigeon
-
-
Manually annotated by BRENDA team
Flavobacterium rhenanum
NCIB 9157, enzyme formed only when amino alcohol serves as a substrate and when activated by ADP
-
-
Manually annotated by BRENDA team
chicken
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Hemidactylus sp.
lizard
-
-
Manually annotated by BRENDA team
catfish
-
-
Manually annotated by BRENDA team
NCIB 8185, enzyme formed only when amino alcohol serves as a substrate and when activated by ADP
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
3D7
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (R)-1-aminopropan-2-ol
ADP + (R)-1-aminopropyl 2-phosphate
show the reaction diagram
ATP + (R)-2-aminobutan-1-ol
ADP + (R)-2-aminobutyl phosphate
show the reaction diagram
ATP + (R)-2-aminopropan-1-ol
ADP + (R)-2-aminopropyl phosphate
show the reaction diagram
ATP + (S)-1-aminopropan-2-ol
ADP + (S)-1-aminopropyl 2-phosphate
show the reaction diagram
ATP + (S)-2-aminobutan-1-ol
ADP + (S)-2-aminobutyl phosphate
show the reaction diagram
ATP + (S)-2-aminopropan-1-ol
ADP + (S)-2-aminopropyl phosphate
show the reaction diagram
ATP + 2-amino-2-methylpropan-1-ol
ADP + 2-amino-2-methylpropan-1-ol phosphate
show the reaction diagram
ATP + 3-aminopropan-1-ol
ADP + 3-aminopropyl 1-phosphate
show the reaction diagram
ATP + 4-aminobutan-1-ol
ADP + 4-aminobutyl 1-phosphate
show the reaction diagram
60% of the activity with choline
-
-
?
ATP + choline
ADP + ?
show the reaction diagram
ATP + choline
ADP + choline phosphate
show the reaction diagram
-
-
-
?
ATP + diethanolamine
ADP + O2-[(2-hydroxyethyl)amino]ethyl phosphate
show the reaction diagram
ATP + DL-1-aminopropan-2-ol
ADP + DL-1-aminopropane 2-phosphate
show the reaction diagram
ATP + ethanolamine
ADP + O-phosphoethanolamine
show the reaction diagram
ATP + N,N-diethylethanolamine
ADP + N,N-diethyl-O-phosphoethanolamine
show the reaction diagram
ATP + N,N-dimethylethanolamine
ADP + N,N-dimethyl-O-phosphoethanolamine
show the reaction diagram
ATP + N-ethylethanolamine
ADP + N-ethyl-O-phosphoethanolamine
show the reaction diagram
ATP + N-methylethanolamine
ADP + N-methyl-O-phosphoethanolamine
show the reaction diagram
choline + ATP
ADP + O-phosphocholine
show the reaction diagram
-
ethanolamine kinase II, not ethanolamine kinase I
-
-
?
GTP + ethanolamine
GDP + O-phosphoethanolamine
show the reaction diagram
-
13% of the activity with ATP
-
-
-
ITP + ethanolamine
IDP + O-phosphoethanolamine
show the reaction diagram
-
45% of the activity with ATP
-
-
-
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + ethanolamine
ADP + O-phosphoethanolamine
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
Flavobacterium rhenanum
-
stimulates
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
100% activity in the presence of 0.0015 mM Zn2+, depletion of zinc from the growth medium of wild type cells causes a 2fold increase in ethanolamine kinase activity when compared with cells grown in the presence of zinc
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
2,3-Dimercaptopropanol
-
-
2-amino-1-butanol
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium]
SAR97276
Acetyl-beta-methylcholine chloride
-
-
Acetylcholine
-
-
betaine
Butyrylcholine iodide
-
-
butyrylthiocholine iodide
-
-
choline
choline phosphate
-
-
cysteine
-
-
EGTA
-
stimulation at 0.4-0.9 mM, inhibition at 1.8 mM
Ethanolaminephosphate
-
-
hemicholinium
-
-
hemicholinium-3
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Inositol
-
decreases gene EKI1 expression, involving the transcription factors Ino2p, Ino4p, and Opi1p, the UASINO element of the EKI promotor is required for the repressive regulation, the inhibitory effect on the enzyme is correlated with a decrease of ethanolamine incorporation into CDP-ethanolamine pathway intermediates and into phosphatidylethanolamine and phosphatidylcholine
Methane sulfonylcholine chloride
-
-
MgATP2-
-
substrate inhibition
monomethylethanolamine
-
-
N,N-dimethylethanolamine
N-ethylmaleimide
-
-
N-Methyl-N-isopropylethanolamine
-
-
N-Methylethanolamine
-
-
Ni2+
-
inhibits activating effect of Mg2+
p-chloromercuribenzoate
phosphorylcholine
Phosphorylethanolamine
Propionylcholine iodide
-
-
SAR97276
bis-thiazolium compound designed as a choline analogue, affects ethanolamine kinase and choline kinase activities similarly. Inhibition is competitive and correlates with the impairment of cellular phosphatidylcholine biosynthesis
stearoyl-CoA
-
-
Succinylcholine chloride
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
stimulates
cysteine
-
stimulates
EGTA
-
stimulation at 0.4-0.9 mM, inhibition at 1.8 mM
glutathione
-
stimulates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0206 - 0.5
ATP
0.02 - 0.275
choline
0.36
DL-1-aminopropan-2-ol
Flavobacterium rhenanum
-
-
0.008 - 10
ethanolamine
1.5 - 10
MgATP2-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00016 - 12.9
ethanolamine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.73
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium]
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.46 - 1.36
2-amino-1-butanol
0.61 - 0.79
3,3'-dodecane-1,12-diylbis[5-(2-hydroxyethyl)-4-methyl-1,3-thiazol-3-ium]
0.46
hemicholinium-3
Plasmodium falciparum
Q8IIB7
pH 8.0, 37C
0.79
SAR97276
Plasmodium falciparum
Q8IIB7
pH 8.0, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.398
-
pH 8.5
10.73
-
pH 8.5
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
-
ethanolamine kinase I
8 - 9.5
-
ethanolamine kinase II
8.5 - 10.5
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 10
-
-
8 - 9
-
80% of maximum activity at pH 8.0 and pH 9.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
during metamorphosis
Manually annotated by BRENDA team
-
germinating
Manually annotated by BRENDA team
-
high expression of isoform Eki2 at the time of sex-differentiation
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17000 - 19000
-
gel filtration, monomeric form
36000 - 37000
-
dimeric form, gel filtration
36000
-
ethanolamine kinase I, gel filtration
44000
-
gel filtration
48000
determined by SDS-PAGE and Western Blot analysis
87000
-
gel filtration
90000
-
gel filtration
100000
gel filtration
109000
determined by gel filtration
110000
-
gel filtration
140000
gel filtration
160000
-
ethanolamine kinase II, gel filtration
190000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
-
1 * 17000-19000, SDS-PAGE, gel filtration, enzyme exists in monomeric and dimeric form
oligomer
-
x * 47000, or x-y * 47000 + y * 43000, or x * 43000, active form consitsts of oligomers of alpha/beta isoforms, with relatively small parts of alpha/alpha or beta/beta homo-oligomers, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
20 min, complete inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivation by repeated freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C or 0-4C, crude cell extract, appreciable loss of activity
-
-20C, 0.02 M phosphate buffer, pH 7.0, 2.9 mM DTT, protein concentration 1.0 mg/ml, ethanolamine kinase I, 2 days, 40% loss of activity
-
-20C, 10 days, stable
-
0-4C or -20C, partially purified enzyme, 10-14 days, 50% loss of activity
-
4C, 1 week, ethanolamine kinase I, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
copurification of choline kinase and ethanolamine kinase
-
ethanolamine kinase I and II
-
recombinant protein; recombinant protein
using a HisTrap chelating and a gel filtration column
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in 293T cells
expressed in Escherichia coli strain strain DH5alpha
-
expression as GST-fusion protein in Escherichia coli strain BL21
-
expression in Escherichia coli
expression in Escherichia coli; expression in Escherichia coli
expression of the enzyme fused to the lacZ reporter gene in several yeast strains and in Escherichia coli strain DH5alpha, expression analysis in presence of inositol and choline in correlation to wild-type and mutated promotor
-
into the vector pET24b for expression in Escherichia coli BL21DE3-pRIL cells
overview
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression levels increases during erythrocytic development
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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