Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0184 | - |
ethanolamine | pH 7.8 | Trypanosoma brucei | |
0.0206 | - |
ATP | pH 7.8 | Trypanosoma brucei | |
0.0314 | - |
choline | - |
Trypanosoma brucei | |
0.219 | - |
ATP | pH 7.8 | Trypanosoma brucei | |
2.56 | - |
ethanolamine | pH 7.8 | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49100 | - |
2 * 49100, calculated, 2 * 51655, MALDI-TOF | Trypanosoma brucei |
51655 | - |
2 * 49100, calculated, 2 * 51655, MALDI-TOF | Trypanosoma brucei |
70140 | - |
2 * 70140, calculated, 2 * 72614, MALDI-TOF | Trypanosoma brucei |
72614 | - |
2 * 70140, calculated, 2 * 72614, MALDI-TOF | Trypanosoma brucei |
100000 | - |
gel filtration | Trypanosoma brucei |
140000 | - |
gel filtration | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q386V0 | isoform EK1 | - |
Trypanosoma brucei | Q57W59 | isoform EK2, bifunctional choline kinase/ethanolamine kinase | - |
Purification (Comment) | Organism |
---|---|
recombinant protein | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-1-aminopropan-2-ol | 52.4% of the activity with ethanolamine | Trypanosoma brucei | ADP + (R)-1-aminopropyl 2-phosphate | - |
? | |
ATP + (R)-1-aminopropan-2-ol | 7.2% of the activity with choline | Trypanosoma brucei | ADP + (R)-1-aminopropyl 2-phosphate | - |
? | |
ATP + (R)-2-aminobutan-1-ol | 102% of the activity with ethanolamine | Trypanosoma brucei | ADP + (R)-2-aminobutyl phosphate | - |
? | |
ATP + (R)-2-aminobutan-1-ol | 130% of the activity with choline | Trypanosoma brucei | ADP + (R)-2-aminobutyl phosphate | - |
? | |
ATP + (R)-2-aminopropan-1-ol | 108% of the activity with ethanolamine | Trypanosoma brucei | ADP + (R)-2-aminopropyl phosphate | - |
? | |
ATP + (R)-2-aminopropan-1-ol | 110% of the activity with choline | Trypanosoma brucei | ADP + (R)-2-aminopropyl phosphate | - |
? | |
ATP + (S)-1-aminopropan-2-ol | 1.4% of the activity with choline | Trypanosoma brucei | ADP + (S)-1-aminopropyl 2-phosphate | - |
? | |
ATP + (S)-1-aminopropan-2-ol | 6.1% of the activity with ethanolamine | Trypanosoma brucei | ADP + (S)-1-aminopropyl 2-phosphate | - |
? | |
ATP + (S)-2-aminobutan-1-ol | 114% of the activity with ethanolamine | Trypanosoma brucei | ADP + (S)-2-aminobutyl phosphate | - |
? | |
ATP + (S)-2-aminobutan-1-ol | 41% of the activity with choline | Trypanosoma brucei | ADP + (S)-2-aminobutyl phosphate | - |
? | |
ATP + (S)-2-aminopropan-1-ol | 22.4% of the activity with choline | Trypanosoma brucei | ADP + (S)-2-aminopropyl phosphate | - |
? | |
ATP + (S)-2-aminopropan-1-ol | 94% of the activity with ethanolamine | Trypanosoma brucei | ADP + (S)-2-aminopropyl phosphate | - |
? | |
ATP + 2-amino-2-methylpropan-1-ol | 25.6% of the activity with ethanolamine | Trypanosoma brucei | ADP + 2-amino-2-methylpropan-1-ol phosphate | - |
? | |
ATP + 2-amino-2-methylpropan-1-ol | 46% of the activity with choline | Trypanosoma brucei | ADP + 2-amino-2-methylpropan-1-ol phosphate | - |
? | |
ATP + 3-aminopropan-1-ol | 37.5% of the activity with ethanolamine | Trypanosoma brucei | ADP + 3-aminopropyl 1-phosphate | - |
? | |
ATP + 3-aminopropan-1-ol | 44.5% of the activity with choline | Trypanosoma brucei | ADP + 3-aminopropyl 1-phosphate | - |
? | |
ATP + 4-aminobutan-1-ol | 60% of the activity with choline | Trypanosoma brucei | ADP + 4-aminobutyl 1-phosphate | - |
? | |
ATP + choline | - |
Trypanosoma brucei | ADP + choline phosphate | - |
? | |
ATP + diethanolamine | - |
Trypanosoma brucei | ADP + O2-[(2-hydroxyethyl)amino]ethyl phosphate | 139% of the activity with choline | ? | |
ATP + diethanolamine | - |
Trypanosoma brucei | ADP + O2-[(2-hydroxyethyl)amino]ethyl phosphate | 88% of the activity with ethanolamine | ? | |
ATP + ethanolamine | - |
Trypanosoma brucei | ADP + O-phosphoethanolamine | - |
? | |
ATP + ethanolamine | 33% of the activity with choline | Trypanosoma brucei | ADP + O-phosphoethanolamine | - |
? | |
ATP + N,N-diethylethanolamine | - |
Trypanosoma brucei | ADP + N,N-diethyl-O-phosphoethanolamine | 156% of the activity with choline | ? | |
ATP + N,N-diethylethanolamine | - |
Trypanosoma brucei | ADP + N,N-diethyl-O-phosphoethanolamine | 47% of the activity with ethanolamine | ? | |
ATP + N,N-dimethylethanolamine | - |
Trypanosoma brucei | ADP + N,N-dimethyl-O-phosphoethanolamine | 153% of the activity with choline | ? | |
ATP + N,N-dimethylethanolamine | - |
Trypanosoma brucei | ADP + N,N-dimethyl-O-phosphoethanolamine | 76% of the activity with ethanolamine | ? | |
ATP + N-ethylethanolamine | - |
Trypanosoma brucei | ADP + N-ethyl-O-phosphoethanolamine | 150% of the activity with choline | ? | |
ATP + N-ethylethanolamine | - |
Trypanosoma brucei | ADP + N-ethyl-O-phosphoethanolamine | 97% of the activity with ethanolamine | ? | |
ATP + N-methylethanolamine | 106% of the activity with ethanolamine | Trypanosoma brucei | ADP + N-methyl-O-phosphoethanolamine | - |
? | |
ATP + N-methylethanolamine | 135% of the activity with choline | Trypanosoma brucei | ADP + N-methyl-O-phosphoethanolamine | - |
? | |
additional information | no choline kinase activity | Trypanosoma brucei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 49100, calculated, 2 * 51655, MALDI-TOF | Trypanosoma brucei |
dimer | 2 * 70140, calculated, 2 * 72614, MALDI-TOF | Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.56 | - |
ethanolamine | pH 7.8 | Trypanosoma brucei | |
12.9 | - |
ethanolamine | pH 7.8 | Trypanosoma brucei |