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Literature summary for 2.7.1.82 extracted from

  • Macher, B.A.; Mudd, J.B.
    Partial purification and properties of ethanolamine kinase from spinach leaf (1976), Arch. Biochem. Biophys., 177, 24-30.
    View publication on PubMed
Search for more literature for 2.7.1.82

Activating Compound

Activating Compound Comment Organism Structure
EGTA stimulation at 0.4-0.9 mM, inhibition at 1.8 mM Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Spinacia oleracea
choline not Spinacia oleracea
EDTA
-
 Spinacia oleracea
EGTA stimulation at 0.4-0.9 mM, inhibition at 1.8 mM Spinacia oleracea
MgATP2- substrate inhibition Spinacia oleracea
monomethylethanolamine
-
 Spinacia oleracea
N,N-dimethylethanolamine
-
 Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 ethanolamine
-
 Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg-ATP complex is the substrate Spinacia oleracea
Mg2+ free Mg2+ essential for maximal velocity Spinacia oleracea
Mg2+ Km MgATP2-: 0.063 mM Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 gel filtration Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Storage Stability

Storage Stability Organism
0-4°C or -20°C, partially purified enzyme, 10-14 days, 50% loss of activity Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + ethanolamine
-
 Spinacia oleracea ADP + O-phosphoethanolamine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Spinacia oleracea