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Information on EC 2.7.1.15 - ribokinase and Organism(s) Homo sapiens

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EC Tree
IUBMB Comments
2-Deoxy-D-ribose can also act as acceptor.
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This record set is specific for:
Homo sapiens
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
ribokinase, sa239, d-ribokinase, rv2436, ms4585, at1g17160, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-ribokinase
-
-
-
-
deoxyribokinase
-
-
-
-
ribokinase (phosphorylating)
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:D-ribose 5-phosphotransferase
2-Deoxy-D-ribose can also act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-84-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-arabinose
?
show the reaction diagram
-
weak activity
-
-
?
ATP + D-arabinose
ADP + D-arabinose 5-phosphate
show the reaction diagram
-
4% if the activity with D-ribose
-
-
?
ATP + D-ribose
ADP + D-ribose 5-phosphate
show the reaction diagram
additional information
?
-
-
no significant phosphorylation of D-fructose, D-galactose and D-xylose
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
may partly support activity
Co2+
about 60% of the activity with Mn2+
Mg2+
about 70% of the activity with Mn2+
Mn2+
best activator, optimum concentration 0.1 mM
Ni2+
may partly support activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-carboxyethylphosphonic acid
-
-
clodronate
-
-
D-ribose
EDTA
complete inhibition
etidronate
-
-
N-(phosphonomethyl)-glycine
-
-
N-(phosphonomethyl)iminodiacetic acid
-
-
Phosphonoacetic acid
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029 - 7.48
ATP
0.08 - 0.12
D-ribose
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01 - 9.8
ATP
0.01 - 10.4
D-ribose
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0013 - 250
ATP
0.08 - 90
D-ribose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 - 18.91
ATP
additional information
additional information
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-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RBSK_HUMAN
322
0
34143
Swiss-Prot
other Location (Reliability: 4)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E202L
mutation in NXXE motif, increase in the Km for MgATP of 52fold, and a decrease in the kcat value of around 200fold
N199L
mutation in NXXE motif, increase in the Km for MgATP- by about 68fold, a decrease in the kcat between 573- and 1036fold and almost no change in the Km for D-ribose. No substrate inhibition by D-ribose
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Park, J.; van Koeverden, P.; Singh, B.; Gupta, R.S.
Identification and characterization of human ribokinase and comparison of its properties with E. coli ribokinase and human adenosine kinase
FEBS Lett.
581
3211-3216
2007
Escherichia coli, Homo sapiens
Manually annotated by BRENDA team
Park, J.; Gupta, R.S.
Adenosine kinase and ribokinase - the RK family of proteins
Cell. Mol. Life Sci.
65
2875-2896
2008
Arabidopsis thaliana, Bos taurus, Burkholderia cepacia, Entamoeba histolytica, Escherichia coli (P0A9J6), Homo sapiens, Leishmania major, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Trypanosoma cruzi
Manually annotated by BRENDA team
Quiroga-Roger, D.; Babul, J.; Guixe, V.
Role of monovalent and divalent metal cations in human ribokinase catalysis and regulation
Biometals
28
401-413
2015
Homo sapiens (Q9H477), Homo sapiens
Manually annotated by BRENDA team