Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E202L | mutation in NXXE motif, increase in the Km for MgATP of 52fold, and a decrease in the kcat value of around 200fold | Homo sapiens |
N199L | mutation in NXXE motif, increase in the Km for MgATP- by about 68fold, a decrease in the kcat between 573- and 1036fold and almost no change in the Km for D-ribose. No substrate inhibition by D-ribose | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens | |
D-ribose | substrate inhibition | Homo sapiens | |
EDTA | complete inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
ATP | wild-type, presence of Mn2+, pH 7.2, 37°C | Homo sapiens | |
0.08 | - |
D-ribose | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
0.12 | - |
D-ribose | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
0.12 | - |
D-ribose | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
0.16 | - |
ATP | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
5.73 | - |
ATP | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
7.48 | - |
ATP | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | may partly support activity | Homo sapiens | |
Co2+ | about 60% of the activity with Mn2+ | Homo sapiens | |
Mg2+ | about 70% of the activity with Mn2+ | Homo sapiens | |
Mn2+ | best activator, optimum concentration 0.1 mM | Homo sapiens | |
Ni2+ | may partly support activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9H477 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose | - |
Homo sapiens | ADP + D-ribose 5-phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
ATP | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
0.01 | - |
D-ribose | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
0.04 | - |
ATP | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
0.04 | - |
D-ribose | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
7.3 | - |
ATP | wild-type, presence of Mn2+, pH 7.2, 37°C | Homo sapiens | |
9.8 | - |
ATP | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
10.4 | - |
D-ribose | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
10.3 | - |
ATP | wild-type, presence of Mn2+, pH 7.2, 37°C | Homo sapiens | |
16.9 | - |
ATP | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
ATP | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
0.007 | - |
ATP | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
0.08 | - |
D-ribose | mutant N199L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
0.5 | - |
D-ribose | mutant E202L, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
60 | - |
ATP | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
90 | - |
D-ribose | wild-type, presence of Mg2+, pH 7.2, 37°C | Homo sapiens | |
250 | - |
ATP | wild-type, presence of Mn2+, pH 7.2, 37°C | Homo sapiens |