Information on EC 2.3.1.74 - naringenin-chalcone synthase

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The expected taxonomic range for this enzyme is: Embryophyta

EC NUMBER
COMMENTARY
2.3.1.74
-
RECOMMENDED NAME
GeneOntology No.
naringenin-chalcone synthase
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
aromatic polyketides biosynthesis
-
Biosynthesis of secondary metabolites
-
Flavonoid biosynthesis
-
flavonoid biosynthesis
-
flavonoid biosynthesis (in equisetum)
-
Metabolic pathways
-
naringenin biosynthesis (engineered)
-
xanthohumol biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
malonyl-CoA:4-coumaroyl-CoA malonyltransferase (cyclizing)
In the presence of NADH and a reductase, 6'-deoxychalcone is produced.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6'-deoxychalcone synthase
-
-
-
-
chalcone synthase
-
-
-
-
chalcone synthase
-
-
chalcone synthase
-
-
chalcone synthase
-
-
chalcone synthase
-
-
chalcone synthase
-
-
chalcone synthase
P30074
-
chalcone synthase
-
-
chalcone synthase
-
-
chalcone synthase
B2NJ30
-
chalcone synthase
A9ZMJ2
-
chalcone synthase
-
-
chalcone synthase
O22122, O48564, Q9LRB2
gene chs
chalcone synthase
C3RTM5
-
chalcone synthase
-
-
chalcone synthase
-
-
chalcone synthase 6
P30080
-
chalcone synthase 7
Q84V87
-
chalcone synthase C2-Idf-I
-
-
chalcone synthase C2-Idf-II
-
-
chalcone synthetase
-
-
-
-
CHS
-
-
-
-
CHS
P30074
-
CHS
B2NJ30
-
CHS
C3RTM5
-
CHS6
P30080
-
CHS7
P30081, Q84V87
-
CHS_H1
-
-
Cs-COR126
-
-
DOCS
-
-
-
-
EC 2.3.1.120
-
-
formerly
-
flavanone synthase
-
-
-
-
flavanone synthetase
-
-
-
-
naringenin-chalcone synthase 6
P30080
-
Q7Y1X9 {SwissProt}
-
-
Q7Y1Y0 {SwissProt}
-
-
RiPKS4
B0LDU5
-
RiPKS5
B0LDU6
-
synthase, flavanone
-
-
-
-
GmIRCHS
Q6X0M8
-
additional information
-
the enzyme is a member of the type III polyketide synthase superfamily
additional information
C3RTM5
the enzyme belongs to a small distinct group of type III polyketide synthases that includes angiosperm and gymnosperm orthologs shown to be anther-specific
CAS REGISTRY NUMBER
COMMENTARY
56803-04-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
ecotype Col-O, gene CHS
-
-
Manually annotated by BRENDA team
cv. Ruby, gene CsCHS-bo
-
-
Manually annotated by BRENDA team
carrot, ssp. sativa
-
-
Manually annotated by BRENDA team
carrot, var Kurdagosun
-
-
Manually annotated by BRENDA team
two distinct activity peaks during fruit ripening at early and late developmental stages; cv. Elsanta
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
L. Merr. cv RCAT Angora (3150 CHU) and cv Harovinton (3100 CHU)
SwissProt
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
barley, inoculated with fungus blumeria graminis
-
-
Manually annotated by BRENDA team
chs_H1 variant, clone No. 132
SwissProt
Manually annotated by BRENDA team
chs_H1 variant, clone No. 1539
SwissProt
Manually annotated by BRENDA team
chs_H1 variant, clone No. 211
SwissProt
Manually annotated by BRENDA team
-
Q9FUB7
SwissProt
Manually annotated by BRENDA team
Juglans sp.
walnut, Juglans nigra * Juglans regia
-
-
Manually annotated by BRENDA team
white-flowering mutant line 18
-
-
Manually annotated by BRENDA team
alfalfa
-
-
Manually annotated by BRENDA team
AB 136, Rio Tibagi, Carioca and Macanudo
-
-
Manually annotated by BRENDA team
Scots pine
-
-
Manually annotated by BRENDA team
MS-Gensuke and ‘Uchiki-Gensuke
SwissProt
Manually annotated by BRENDA team
cultivar: Royalty
SwissProt
Manually annotated by BRENDA team
cv. Royalty, raspberry
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
ringworm bush
-
-
Manually annotated by BRENDA team
Silene sp.
-
-
-
Manually annotated by BRENDA team
white mustard
-
-
Manually annotated by BRENDA team
genotype alpine and prairie
-
-
Manually annotated by BRENDA team
cv. AC Barrie, AABBDD, chalcone synthase-like gene CHSL1
UniProt
Manually annotated by BRENDA team
Verbena sp.
-
-
-
Manually annotated by BRENDA team
cv. Cabernet Sauvigon
-
-
Manually annotated by BRENDA team
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
CaCl2
Juglans sp.
-
1 mM, 15% increase of activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
Juglans sp.
-
activation
2-mercaptoethanol
-
also leads to increased formation of by-products
2-mercaptoethanol
-
2fold increase at 20 mM
2-mercaptoethanol
-
activation
Bovine serum albumin
-
-
-
Bovine serum albumin
-
activation
-
chlorogenic acid
-
increases eriodictyol formation by 20%
cysteine
Juglans sp.
-
dependent on
dithiothreitol
-
4fold increase at 20 mM
Dowex
Juglans sp.
-
77% increase of activity
-
EDTA
Juglans sp.
-
50 mM, 9% increase of activity
N2
Juglans sp.
-
79% increase of activity
Polyethyleneglycol
Juglans sp.
-
activity strongly dependent on 1.5%
Polyvinylpyrrolidone
Juglans sp.
-
10% w/v essential
Sucrose
Juglans sp.
-
25% increase of activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
-
Juglans sp.
-
-
45
-
-
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
50
-
less than 50% of maximal activity above and below
20
60
-
50% activity at 20°C, 50°C and 60°C
25
45
Juglans sp.
-
less than 40% of maximal activity above and below
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.79
-
O22122, O48564, Q9LRB2
sequence calculation
5.86
-
O22122, O48564, Q9LRB2
sequence calculation; sequence calculation
7.1
-
-
calculated
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0
-
-
half-life 3 h in 1.4 mM 2-mercaptoethanol, increase of stability with 14 mM 2-mercaptoethanol
4
-
-
half-life 4-5 d, partially purified enzyme
25
-
-
50% inactivation after 90 min
25
-
-
stable up to
100
-
-
boiling for 10 min leads to complete inactivity
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
withdrawal of 2-mercaptoethanol after ammonium sulfate precipitation leads to increased stability during further purification
-
0.1% bovine serum albumin stabilizes
Juglans sp.
-
5% activity after freezing for 1 h without presence of glycerol
-
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
sensitive to oxygen
Juglans sp.
-
487454
exclusion of oxygen during purification gives improved yield and purity of product
-
487465
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
biotechnology
-
Agrobacterium-mediated infection of Petunia hybrida plants with tobacco rattle virus bearing fragments of Petunia genes results in systemic infection and virus-induced gene silencing of the homologous host genes. Infection with TRV containing a chalcone synthase fragment results in silencing of anthocyanin production in infected flowers. Value of virus-induced gene silencing with tandem constructs containing CHS as reporter and a target gene as a tool for examining the function of floral-associated genes