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0.003 - 0.37
3'-phosphoadenosine 5'-phosphosulfate
0.06
adenosine 5'-O-(3-thiotriphosphate)
-
pH 8.0
0.00014 - 0.042
adenosine 5'-phosphosulfate
0.00044 - 0.00072
adenosine-5'-phosphosulfate
0.002 - 0.0046
adenylyl sulfate
additional information
additional information
-
0.003
3'-phosphoadenosine 5'-phosphosulfate
pH 8.1, 30°C, wild-type enzyme
0.008
3'-phosphoadenosine 5'-phosphosulfate
-
pH 8.0, 30°C
0.039
3'-phosphoadenosine 5'-phosphosulfate
pH 8.1, 30°C, S107A mutant enzyme
0.37
3'-phosphoadenosine 5'-phosphosulfate
-
-
0.37
3'-phosphoadenosine 5'-phosphosulfate
-
pH 8.0, 25°C
0.00014
adenosine 5'-phosphosulfate
pH 7.4, 25°C
0.00025
adenosine 5'-phosphosulfate
-
pH 8.0, 25°C
0.0004
adenosine 5'-phosphosulfate
pH 8.0, 37°C, COS-1 cell-expressed full length PAPS synthase
0.00043
adenosine 5'-phosphosulfate
-
oxidized wild type enzyme, at pH 7.5 and 17°C
0.00043
adenosine 5'-phosphosulfate
oxidized wild type enzyme, at pH 7.5 and 25°C
0.00045
adenosine 5'-phosphosulfate
-
-
0.00048
adenosine 5'-phosphosulfate
-
reduced wild type enzyme, at pH 7.5 and 17°C
0.00048
adenosine 5'-phosphosulfate
reduced wild type enzyme, at pH 7.5 and 25°C
0.00048
adenosine 5'-phosphosulfate
wild type enzyme, at pH 7.5 and 17°C
0.0005
adenosine 5'-phosphosulfate
-
pH 8.0
0.0006
adenosine 5'-phosphosulfate
pH 8.0, 37°C, COS-1 cell-expressed N-terminal fragment
0.0007
adenosine 5'-phosphosulfate
mutant enzyme D136N, at pH 7.5 and 17°C
0.0008
adenosine 5'-phosphosulfate
-
pH 8.1, 30°C
0.00087
adenosine 5'-phosphosulfate
oxidized mutant enzyme C86A/C119A, at pH 7.5 and 25°C
0.00092
adenosine 5'-phosphosulfate
mutant enzyme D136A, at pH 7.5 and 17°C
0.001
adenosine 5'-phosphosulfate
pH 8.1, 30°C, wild-type enzyme at 100 mM ammonium sulfate
0.0014
adenosine 5'-phosphosulfate
-
-
0.0014
adenosine 5'-phosphosulfate
-
pH 8.0, 30°C
0.00172
adenosine 5'-phosphosulfate
-
mutant enzyme R93A, at pH 7.5 and 17°C
0.0019
adenosine 5'-phosphosulfate
reduced mutant enzyme C86A/C119A, at pH 7.5 and 25°C
0.002
adenosine 5'-phosphosulfate
-
pH 6.8, 30°C
0.0023
adenosine 5'-phosphosulfate
pH 8.0, 37°C, Escherichia coli-expressed N-terminal fragment
0.0026
adenosine 5'-phosphosulfate
pH 8.0, 37°C, Escherichia coli-expressed full length PAPS synthase
0.00263
adenosine 5'-phosphosulfate
-
N-terminal truncation variant APSKDELTA96, at pH 7.5 and 17°C
0.0036
adenosine 5'-phosphosulfate
25°C
0.0042
adenosine 5'-phosphosulfate
pH 8.0, 30°C
0.006
adenosine 5'-phosphosulfate
-
pH 8.0
0.012
adenosine 5'-phosphosulfate
pH 8.1, 30°C, S107A mutant enzyme at 100 mM ammonium sulfate
0.042
adenosine 5'-phosphosulfate
-
pH 8.0, 30°C
0.00044
adenosine-5'-phosphosulfate
-
mutant enzyme C36A/C69A, at pH 8.0 and 25°C
0.00072
adenosine-5'-phosphosulfate
-
wild type enzyme, at pH 8.0 and 25°C
0.002
adenylyl sulfate
value is below 0.002 for a deletion mutant lacking the first 34 amino acids of the N-terminus, delta34N
0.002
adenylyl sulfate
value is below 0.002 for wildtype adenylyl sulfate
0.0032
adenylyl sulfate
mutant R37A
0.0037
adenylyl sulfate
mutant R40A
0.0046
adenylyl sulfate
deletion mutant lacking the first 50 amino acids of the N-terminus, delta50N
0.13
ADP
-
pH 8.0, 25°C
0.13
ADP
-
ADP in form of MgADP-
0.007
ATP
-
pH 6.8, 30°C
0.08
ATP
-
pH 8.0, 37°C, PAPSS 1
0.14
ATP
-
mutant enzyme C36A/C69A, at pH 8.0 and 25°C
0.14
ATP
ATP in form of MgATP2-
0.23
ATP
pH 8.0, 37°C, COS-1 cell-expressed N-terminal fragment
0.26
ATP
pH 8.0, 37°C, E. coli expressed N-terminal fragment
0.36
ATP
-
pH 8.0, 37°C, PAPSS 2b
0.38
ATP
-
pH 8.0, 37°C, PAPSS 2a
0.45
ATP
pH 8.0, 37°C, E. coli-expressed full length PAPS synthase
0.46
ATP
-
at pH 8.0 and 25°C
0.75
ATP
-
wild type enzyme, at pH 8.0 and 25°C
0.8
ATP
pH 8.1, 30°C, wild-type enzyme at 100 mM ammonium sulfate
0.8
ATP
-
ATP in form of MgATP2-
0.8
ATP
ATP in form of MgATP2-
2.4
ATP
pH 8.1, 30°C, S107A mutant enzyme
2.4
ATP
ATP in form of MgATP2-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study
-
additional information
additional information
-
kinetic study
-