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2.3.1.157: glucosamine-1-phosphate N-acetyltransferase

This is an abbreviated version!
For detailed information about glucosamine-1-phosphate N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.157

Reaction

acetyl-CoA
+
alpha-D-glucosamine 1-phosphate
=
CoA
 +
N-acetyl-alpha-D-glucosamine 1-phosphate

Synonyms

amino-sugar-1-P AcTase, amino-sugar-1-phosphate acetyltransferase, bifunctional GlmU protein, bifunctional N-acetyltransferase/uridylyltransferase, bifunctional protein GlmU, bifunctional UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase, galactosamine-1-phosphate acetyltransferase, GalN-1-P AcTase, GlcN-1-P acetyltransferase, GlcN-1-P AcTase, GlcNAc-1-P uridyltransferase, GlmU, GlmU acetyltransferase, GlmU enzyme, GlmU uridyltransferase, glucosamine 1-phosphate N-acetyltransferase/N-acetylglucosamine-1-phosphate uridyltransferase, glucosamine-1-phosphate acetyltransferase, glucosamine-1-phosphate acetyltransferase/N-acetylglucosamine-1-phosphate uridyltransferase, More, MtbGlmU, MtGlmU, N-acetylglucosamine-1-phosphate pyrophosphorylase, N-acetylglucosamine-1-phosphate uridyltransferase, N-acetylglucosamine-1-phosphate-uridyltransferase/glucosamine-1-phosphate-acetyltransferase, N-acetylglucosamine-1-phosphate-uridylyltransferase/glucosamine-1-phosphate-acetyltransferase, Rv1018c, Rxn-1, ST0452, ST0452 protein, STK_04520, UDP-GlcNAc pyrophosphorylase, UDP-N-acetylglucosamine pyrophosphorylase/glucosamine-1-phosphate N-acetyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.157 glucosamine-1-phosphate N-acetyltransferase

Purification

Purification on EC 2.3.1.157 - glucosamine-1-phosphate N-acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
GlmU gene product, bifunctional enzyme with glucosamine-1-phosphate acetyltransferase and uridyltransferase activity
-
Ni-NTA His-binding column chromatography
recombinant GlmU from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
recombinant GlmU from Escherichia coli strain HMS174(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL12(DE3) by cobalt affinity chromatography, overexpression of GlmU in Mycobacterium tuberculosis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant N-terminally His-tagged wild-type GlmU and mutant GlmUs from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
the recombinant protein by Ni2+ affinity chromatography
using a GSTrap glutathione Sepharose column. The GST tag is removed by proteolytic cleavage using recombinant tobacco etch virus protease
-
using Ni-NTA chromatography. The His6 tag is cleaved by incubation with recombinant tobacco etch virus (rTEV) protease