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1.8
acetoacetyl-CoA
pH 7.5, 30°C
0.66 - 1.71
alpha-D-galactosamine 1-phosphate
0.008 - 1.69
alpha-D-glucosamine 1-phosphate
0.000009
CoA
-
pH 7.5, 25°C
0.07 - 0.25
D-glucosamine 1-phosphate
0.003
N-acetyl-alpha-D-glucosamine 1-phosphate
-
pH 7.5, 25°C
0.07
N-acetyl-D-glucosamine 1-phosphate
-
uridyltransferase activity
0.06
n-propionyl-CoA
pH 7.5, 30°C
0.06
propionyl-CoA
pH 7.5, 30°C
1.1
succinyl-CoA
pH 7.5, 30°C
0.1
UTP
-
uridyltransferase activity
additional information
additional information
-
0.056
acetyl-CoA
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant K403A
0.06
acetyl-CoA
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant E458A
0.07
acetyl-CoA
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant S416A
0.09
acetyl-CoA
-
mutant His6-GlmU C307A
0.14
acetyl-CoA
-
mutant His6-GlmU C324A
0.199
acetyl-CoA
mutant enzyme V385C/C405S, at pH 8.0 and 37°C
0.2
acetyl-CoA
-
mutant His6GlmU, mutant His6-GlmU C296A, mutant His6-GlmU C385A
0.224
acetyl-CoA
-
pH 7.5, 37°C
0.224
acetyl-CoA
-
pH 7.5, 25°C
0.224
acetyl-CoA
-
pH and temperature not specified in the publication, recombinant His-tagged wild-type GlmU
0.23
acetyl-CoA
pH 7.5, 37°C, recombinant enzyme
0.23
acetyl-CoA
pH not specified in the publication, temperature not specified in the publication
0.242
acetyl-CoA
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant S474A
0.25
acetyl-CoA
pH 7.5, 30°C
0.25
acetyl-CoA
-
mutant His6-GlmU C385A
0.3
acetyl-CoA
pH 7.6, 30°C, recombinant mutant H374A
0.304
acetyl-CoA
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant R463A
0.343
acetyl-CoA
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant N456A
0.355
acetyl-CoA
pH 7.6, 30°C, recombinant mutant N397A
0.355
acetyl-CoA
pH 7.6, 30°C, recombinant mutant S416A
0.355
acetyl-CoA
pH 7.6, 30°C, recombinant wild-type
0.52
acetyl-CoA
-
pH 8.2, 37°C
0.52
acetyl-CoA
pH 7.5, 80°C, mutant enzyme DC005 with a deletion of the C-terminal 5 residues of the ST0452 protein
0.52
acetyl-CoA
pH 7.5, 80°C, deletion mutant D005 of ST0452 protein
0.52
acetyl-CoA
-
pH 8.2, 37°C, recombinant His6-tagged enzyme
0.603
acetyl-CoA
mutant enzyme C380A, at pH 8.0 and 37°C
0.63
acetyl-CoA
pH 7.5, 80°C
0.63
acetyl-CoA
pH 7.5, 80°C, wild-type enzyme
0.63
acetyl-CoA
pH 7.5, 80°C, wild-type ST0452 protein
0.638
acetyl-CoA
mutant enzyme C380A/C405S, at pH 8.0 and 37°C
1.213
acetyl-CoA
mutant enzyme C405A, at pH 8.0 and 37°C
1.55
acetyl-CoA
pH 7.5, 80°C, mutant enzyme DC011 with a deletion of the C-terminal 11 residues of the ST0452 protein
1.55
acetyl-CoA
pH 7.5, 80°C, deletion mutant D011 of ST0452 protein
1.821
acetyl-CoA
wild type enzyme, at pH 8.0 and 37°C
1.963
acetyl-CoA
mutant enzyme C405S, at pH 8.0 and 37°C
0.66
alpha-D-galactosamine 1-phosphate
pH 7.5, 80°C, deletion mutant D011 of ST0452 protein
0.84
alpha-D-galactosamine 1-phosphate
pH 7.5, 80°C, deletion mutant D005 of ST0452 protein
1.71
alpha-D-galactosamine 1-phosphate
pH 7.5, 80°C, wild-type ST0452 protein
0.008
alpha-D-glucosamine 1-phosphate
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant K403A
0.016
alpha-D-glucosamine 1-phosphate
cosubstrate acetoacetyl-CoA, pH 7.5, 30°C
0.016
alpha-D-glucosamine 1-phosphate
pH 7.5, 30°C, cosubstrate: acetoacetyl-CoA
0.044
alpha-D-glucosamine 1-phosphate
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant N456A
0.061
alpha-D-glucosamine 1-phosphate
-
pH 7.5, 37°C
0.061
alpha-D-glucosamine 1-phosphate
-
pH 7.5, 25°C
0.061
alpha-D-glucosamine 1-phosphate
-
pH and temperature not specified in the publication, recombinant His-tagged wild-type GlmU
0.066
alpha-D-glucosamine 1-phosphate
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant E458A
0.069
alpha-D-glucosamine 1-phosphate
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant R463A
0.073
alpha-D-glucosamine 1-phosphate
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant S474A
0.107
alpha-D-glucosamine 1-phosphate
pH 7.5, 37°C, recombinant enzyme
0.107
alpha-D-glucosamine 1-phosphate
pH not specified in the publication, temperature not specified in the publication
0.13
alpha-D-glucosamine 1-phosphate
-
pH and temperature not specified in the publication, recombinant His-tagged GlmU mutant S416A
0.241
alpha-D-glucosamine 1-phosphate
mutant enzyme C380A, at pH 8.0 and 37°C
0.278
alpha-D-glucosamine 1-phosphate
mutant enzyme C405A, at pH 8.0 and 37°C
0.283
alpha-D-glucosamine 1-phosphate
pH 7.6, 30°C, recombinant mutant N397A
0.29
alpha-D-glucosamine 1-phosphate
pH 7.5, 30°C
0.29
alpha-D-glucosamine 1-phosphate
cosubstrate acetyl-CoA, pH 7.5, 30°C
0.3
alpha-D-glucosamine 1-phosphate
-
pH 8.2, 37°C
0.3
alpha-D-glucosamine 1-phosphate
pH 7.6, 30°C, recombinant mutant H374A
0.3
alpha-D-glucosamine 1-phosphate
-
pH 8.2, 37°C, recombinant His6-tagged enzyme
0.304
alpha-D-glucosamine 1-phosphate
mutant enzyme V385C/C405S, at pH 8.0 and 37°C
0.333
alpha-D-glucosamine 1-phosphate
wild type enzyme, at pH 8.0 and 37°C
0.353
alpha-D-glucosamine 1-phosphate
pH 7.6, 30°C, recombinant wild-type
0.359
alpha-D-glucosamine 1-phosphate
mutant enzyme C405S, at pH 8.0 and 37°C
0.37
alpha-D-glucosamine 1-phosphate
pH 7.6, 30°C, recombinant mutant S416A
0.47
alpha-D-glucosamine 1-phosphate
mutant enzyme C380A/C405S, at pH 8.0 and 37°C
0.56
alpha-D-glucosamine 1-phosphate
pH 7.5, 80°C, mutant enzyme DC005 with a deletion of the C-terminal 5 residues of the ST0452 protein
0.56
alpha-D-glucosamine 1-phosphate
pH 7.5, 80°C, deletion mutant D005 of ST0452 protein
0.59
alpha-D-glucosamine 1-phosphate
pH 7.5, 80°C
0.59
alpha-D-glucosamine 1-phosphate
pH 7.5, 80°C, wild-type enzyme
0.59
alpha-D-glucosamine 1-phosphate
pH 7.5, 80°C, wild-type ST0452 protein
0.98
alpha-D-glucosamine 1-phosphate
cosubstrate propionyl-CoA, pH 7.5, 30°C
0.98
alpha-D-glucosamine 1-phosphate
pH 7.5, 30°C, cosubstrate: n-propionyl-CoA
1.69
alpha-D-glucosamine 1-phosphate
pH 7.5, 80°C, mutant enzyme DC011 with a deletion of the C-terminal 11 residues of the ST0452 protein
1.69
alpha-D-glucosamine 1-phosphate
pH 7.5, 80°C, deletion mutant D011 of ST0452 protein
0.07
D-glucosamine 1-phosphate
-
mutant His6-GlmU C296A
0.12
D-glucosamine 1-phosphate
-
mutant His6-GlmU C324A
0.15
D-glucosamine 1-phosphate
-
-
0.2
D-glucosamine 1-phosphate
-
mutant His6GlmU
0.24
D-glucosamine 1-phosphate
-
Vmax: 4.489 microM/min/pmol enzyme
0.25
D-glucosamine 1-phosphate
-
mutant His6-GlmU C307A
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
kinetic modelling of GlmU, simulation of the model, detailed overview. Estimated and measured values are very coherent
-
additional information
additional information
-
steady-state kinetics and kinetic analysis of the acetyltransferase activity of GlmU, overview
-