1.1.1.325: sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming)
This is an abbreviated version!
For detailed information about sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming), go to the full flat file.
Reaction
Synonyms
MDSPR, sepiapterin reductase, SPR
ECTree
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Purification
Purification on EC 1.1.1.325 - sepiapterin reductase (L-threo-7,8-dihydrobiopterin forming)
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native enzyme 533fold from cytosol by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration followed by affinity chromatography, another different step of anion exchange chromatography, and ultrafiltration
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)
recombinant His-tagged wild-type and selenomethionine-substituted enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, anion exchange chromatography, and gel filtration
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