EC Number   |
Protein Variants |
Reference |
|---|
   3.4.22.56 | C143A |
inactive enzyme. Catalytically inactive p17 polypeptide is expressed in a stable manner, while wild-type p17 is rapidly degraded |
667192 |
| 3.4.22.56 | C163G |
transfection of human breast cancer cells lacking enzyme with wild-type or mutant cDNA. Expression of wild-type, but not the inactive mutants C163G or C163S, is associated with increased enzyme activity and susceptibility to staurosporine-induced apoptosis. Wild-type and mutant transfection results in inhibition of cell growth and decrease in phosphorylation of the Akt protein compared to control |
669603 |
| 3.4.22.56 | C163S |
transfection of human breast cancer cells lacking enzyme with wild-type or mutant cDNA. Expression of wild-type, but not the inactive mutants C163G or C163S, is associated with increased enzyme activity and susceptibility to staurosporine-induced apoptosis. Wild-type and mutant transfection results in inhibition of cell growth and decrease in phosphorylation of the Akt protein compared to control |
669603 |
| 3.4.22.56 | D179A |
increase both in kcat- and KM-value. D179 is involved in substrate recognition |
669881 |
| 3.4.22.56 | DELTA176-181 |
decrease both in kcat- and KM-value |
669881 |
| 3.4.22.56 | more |
caspase-3 gene silencing leads to inhibition of apoptosis in insulinoma cells and human islets, caspase-3 upregulation leads to the generation of active caspase-6 and caspase-7, which in turn degrade a number of intracellular protein substrates |
700291 |
| 3.4.22.56 | more |
caspase-3 knockout by shRNA, generation of double knockdown cells lacking caspase-3 and caspase-7, EC 3.4.22.60, activity. Generation of caspase-7-based chimeric constructs with various regions of caspase-3, EC 3.4.22.56 |
717796 |
| 3.4.22.56 | more |
construction of a caspase-3/-7 double-knockout mouse embryonic fibroblasts cell model for studying of autophagy contributing to the mechanism of increased cell death, following inhibition of apoptosis |
700881 |
| 3.4.22.56 | more |
construction of an in vivo mouse hind limb H460 lung cancer model |
700881 |
| 3.4.22.56 | more |
engineering of a light-activated human caspase-3 (Caspase-LOV) by exploiting its natural spring-loaded activation mechanism through rational insertion of the light-sensitive LOV2 domain that expands upon illumination |
755264 |
