EC Number   |
Protein Variants |
Reference |
|---|
    3.2.1.22 | A156V |
reduced activity |
678069 |
| 3.2.1.22 | A20P |
reduced activity |
678069 |
| 3.2.1.22 | A230T |
6 mM 2,6-dithiopurine promotes the maturation of the mutant enzyme |
751953 |
| 3.2.1.22 | A37T |
2,6-dithiopurine has no effect on this mutant enzyme |
751953 |
| 3.2.1.22 | A41Y |
kcat decreased compared to wild-tpye, Km (substrate: 4-nitrophenyl-alpha-D-galactopyranoside) or (substrate: melibiose) decreased compared to wild-type, Km (raffinose) increased compared to wild-type |
715537 |
| 3.2.1.22 | A97V |
reduced activity |
678069 |
| 3.2.1.22 | C142W |
mutation isolated in patient with Fabry disease. 1-Deoxygalactonojirimycin has no significant effect on catalytic activity |
697800 |
| 3.2.1.22 | C159A |
mutant enzyme shows the same specific activity against p-nitrophenyl alpha-D-galactopyranoside as the wild-type enzyme. 1 mM PCMB has no effect in activity of the mutant enzyme, wild-type enzyme is completely inhibited |
654253 |
| 3.2.1.22 | C172G |
2,6-dithiopurine has no effect on this mutant enzyme |
751953 |
| 3.2.1.22 | D149A |
mutant shows no activity |
715537 |
