Information on EC 3.2.1.22 - alpha-galactosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY
3.2.1.22
-
RECOMMENDED NAME
GeneOntology No.
alpha-galactosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
show the reaction diagram
also hydrolyses alpha-D-fucosides
-
-
-
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
show the reaction diagram
the recombinant enzyme catalyzes the hydrolysis of phenyl R-galactosides via a redox elimination-addition mechanism involving oxidation of the hydroxyl group at C-3 and elimination of phenol across the C-1-C-2 bond to give an enzyme-bound glycal intermediate
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Galactose metabolism
-
Glycerolipid metabolism
-
Glycosphingolipid biosynthesis - globo series
-
melibiose degradation
-
Sphingolipid metabolism
-
stachyose degradation
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
a-galactosidase
-
-
Aga-F78
Rhizopus sp.
-
-
Aga-F78
C7SEV1
-
Aga-F78
Rhizopus sp. F78
-
-
-
AgalB
Bispora sp.
-
-
Agalsidase alfa
-
-
-
-
Agalsidase alfa
-
-
AkalphaGal
-
-
alkaline alpha-gal form 1
-
-
alkaline alpha-galactosidase
-
-
alkaline alpha-galactosidase
-
-
alkaline alpha-galactosidase form 1
-
-
alpha-D-galactopyranoside galactohydrolase
-
-
alpha-D-galactopyranoside galactohydrolase
Talaromyces flavus CCF2686
-
-
-
alpha-D-galactosidase
-
-
-
-
alpha-D-galactosidase
-
-
alpha-D-galactosidase
-
-
alpha-D-galactosidase
Q19AX0
-
alpha-D-galactosidase
-
-
alpha-D-galactosidase
Talaromyces flavus CCF2686
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
Aspergillus niger M47
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
Geobacillus stearothermophilus NCIM 5146, Geobacillus stearothermophilus NCIM-5146
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
Penicillium chrysogenum LN33
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
Q19AX0
-
Alpha-D-galactoside galactohydrolase
-
-
alpha-Gal
-
-
alpha-Gal
-
-
alpha-Gal
-
-
alpha-Gal
Q97U94
-
alpha-Gal
Q97U94
-
-
alpha-Gal II
-
-
alpha-Gal III
Q7XIV4
-
alpha-gal1
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
Bispora sp.
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
Q7WWP9
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
P04824
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
Thalassococcus halodurans UST050418-052
-
-
-
alpha-galactosidase 1
-
-
alpha-galactosidase 2
-
-
alpha-galactosidase 3
-
-
alpha-galactosidase A
-
-
-
-
alpha-galactosidase A
-
-
alpha-galactosidase AgaA A355E
-
-
alpha-galactosidase AgaB
-
-
alpha-galactosidase I
Q02402
-
alpha-galactosidase II
-
-
alpha-galactosidase III
Q7XIV4
-
alpha-galactoside galactohydrolase
-
-
-
-
alpha-galactoside galactohydrolase
-, Q7XIV4
-
alphaGal1
-
-
alphaGal1
Talaromyces flavus CCF2686
-
-
-
E1 alpha-galactosidase
-
-
E2 alpha-galactosidase
-
-
E3 alpha-galactosidase
-
-
GalS
Q97U94
-
-
GH36 alpha-galactosidase
-
-
LaMel36A
Q7WWP9
-
Mel4A
Q9KAQ9
-
MelA
-
gene name
melibiase
-
-
-
-
retaining alpha-galactosidase
-
-
Tm GalA
O33835
-
TmGalA
Thermotoga maritima DSMZ3109
-
-
-
TnGalA
Thermotoga neapolitana DSMZ5068
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9025-35-8
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Absidia sp.
strain WL511
-
-
Manually annotated by BRENDA team
Absidia sp. WL511
strain WL511
-
-
Manually annotated by BRENDA team
type Runner and type Spanish
-
-
Manually annotated by BRENDA team
NRRL 3135
-
-
Manually annotated by BRENDA team
Aspergillus ficuum NRRL 3135
NRRL 3135
-
-
Manually annotated by BRENDA team
strain M47
-
-
Manually annotated by BRENDA team
Aspergillus niger M47
strain M47
-
-
Manually annotated by BRENDA team
Aspergillus paxillus
-
-
-
Manually annotated by BRENDA team
growth on guar gum as sole carbon source
-
-
Manually annotated by BRENDA team
strain ATCC 29148
Uniprot
Manually annotated by BRENDA team
Bifidobacterium animalis subsp. lactis LAFTI B94
strain LAFTI B94
-
-
Manually annotated by BRENDA team
strain 203
-
-
Manually annotated by BRENDA team
strain 203
Uniprot
Manually annotated by BRENDA team
Bifidobacterium breve 203
strain 203
-
-
Manually annotated by BRENDA team
Bifidobacterium breve 203
strain 203
Uniprot
Manually annotated by BRENDA team
strain LAFTI Bl536
-
-
Manually annotated by BRENDA team
Bifidobacterium longum LAFTI Bl536
strain LAFTI Bl536
-
-
Manually annotated by BRENDA team
Bispora sp.
-
-
-
Manually annotated by BRENDA team
Cajanus indicus
-
-
-
Manually annotated by BRENDA team
Calvatia cyanthiformis
-
-
-
Manually annotated by BRENDA team
Candida javanica
-
-
-
Manually annotated by BRENDA team
chestnut
-
-
Manually annotated by BRENDA team
Citrullus battich
watermelon
-
-
Manually annotated by BRENDA team
existence of at two isoforms, encoded by at least two genes
-
-
Manually annotated by BRENDA team
var. Robusta
Uniprot
Manually annotated by BRENDA team
Coffea sp.
green coffee beans
-
-
Manually annotated by BRENDA team
Corynebacterium murisepticum
-
-
-
Manually annotated by BRENDA team
a cold-tolerant cultivar, Guonong No. 25, and a cold sensitive cultivar, Guonong No. 41
-
-
Manually annotated by BRENDA team
strain UFV-1
-
-
Manually annotated by BRENDA team
Debaryomyces hansenii UFV-1
strain UFV-1
-
-
Manually annotated by BRENDA team
alpha-galactosidase AgaA; alpha-galactosidase AgaB
-
-
Manually annotated by BRENDA team
strain NCIM 5146
-
-
Manually annotated by BRENDA team
strain NCIM-5146
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus NCIM 5146
strain NCIM 5146
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus NCIM-5146
strain NCIM-5146
-
-
Manually annotated by BRENDA team
Gibberella sp.
strain F75
UniProt
Manually annotated by BRENDA team
strain F75
UniProt
Manually annotated by BRENDA team
cultivar Monarca
-
-
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
L. cv. Giant Gray Stripe
-
-
Manually annotated by BRENDA team
male patients with Fabry disease
-
-
Manually annotated by BRENDA team
patients with Fabry disease
-
-
Manually annotated by BRENDA team
patients with sporadic Parkinson's disease
-
-
Manually annotated by BRENDA team
Hypocrea jecorina RUT C-30
RUT C-30
-
-
Manually annotated by BRENDA team
strains LAFTI L10 and La4962
-
-
Manually annotated by BRENDA team
strains LAFTI L26 and Lc279
-
-
Manually annotated by BRENDA team
strain R08, high alpha-galactosidase activity during the stationary phase in a medium where raffinose is the only carbon source
-
-
Manually annotated by BRENDA team
Lactobacillus curvatus R08
strain R08, high alpha-galactosidase activity during the stationary phase in a medium where raffinose is the only carbon source
-
-
Manually annotated by BRENDA team
subspecies Lactobacillus delbrueckii bulgaricus, strain Lb1466
-
-
Manually annotated by BRENDA team
strain CRL722
Uniprot
Manually annotated by BRENDA team
Lactobacillus fermentum CRL722
strain CRL722
Uniprot
Manually annotated by BRENDA team
strain JK55, high alpha-galactosidase activity during the stationary phase in a medium where raffinose is the only carbon source
-
-
Manually annotated by BRENDA team
Leuconostoc mesenteroides JK55
strain JK55, high alpha-galactosidase activity during the stationary phase in a medium where raffinose is the only carbon source
-
-
Manually annotated by BRENDA team
no activity in Thalassobius aestuarii
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius aestuarii JC2049
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius gelatinovorus
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius gelatinovorus IAM 12617
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius mediterraneus
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius mediterraneus XSM19
-
-
-
Manually annotated by BRENDA team
alpha-Gal II; variant Nipponbare
Uniprot
Manually annotated by BRENDA team
L. var. Nipponbare
Uniprot
Manually annotated by BRENDA team
variant Nipponbare
Q7XIV4
UniProt
Manually annotated by BRENDA team
Penicillium chrysogenum LN33
strain LN33
-
-
Manually annotated by BRENDA team
Penicillium duponti
-
-
-
Manually annotated by BRENDA team
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
-
-
Manually annotated by BRENDA team
VKM B-2135 D
UniProt
Manually annotated by BRENDA team
Rhizopus sp.
18S rRNA sequence; strain F78 ACCC 30795
-
-
Manually annotated by BRENDA team
Rhizopus sp. F78
18S rRNA sequence; strain F78 ACCC 30795
-
-
Manually annotated by BRENDA team
; alpha-galactosidase precursor
SwissProt
Manually annotated by BRENDA team
sugar cane
-
-
Manually annotated by BRENDA team
Solanum lycopersicum Mill.
Mill.
SwissProt
Manually annotated by BRENDA team
Streptococcus thermophilus St1342
strain St1342
-
-
Manually annotated by BRENDA team
9917S2; 9929S1, 0177SA12, 096S14, 098SA1, 053A1
-
-
Manually annotated by BRENDA team
Streptomyces olivaceus 9917S2
9917S2
-
-
Manually annotated by BRENDA team
Tachigali multijuga
-
-
-
Manually annotated by BRENDA team
strain CCF2686
-
-
Manually annotated by BRENDA team
Talaromyces flavus CCF2686
strain CCF2686
-
-
Manually annotated by BRENDA team
UST050418-052
-
-
Manually annotated by BRENDA team
Thalassococcus halodurans UST050418-052
UST050418-052
-
-
Manually annotated by BRENDA team
strain CBS 395.62/b
-
-
Manually annotated by BRENDA team
Thermomyces lanuginosus CBS 395.62/b
strain CBS 395.62/b
-
-
Manually annotated by BRENDA team
Thermomyces lanuginosus IMI 158749
IMI 158749
-
-
Manually annotated by BRENDA team
strain MSB8
Uniprot
Manually annotated by BRENDA team
Thermotoga maritima DSMZ3109
DSMZ3109
-
-
Manually annotated by BRENDA team
Thermotoga neapolitana 5068
5068
-
-
Manually annotated by BRENDA team
Thermotoga neapolitana DSMZ5068
DSMZ5068
-
-
Manually annotated by BRENDA team
synonym Mortierella vinacea
UniProt
Manually annotated by BRENDA team
mung bean
-
-
Manually annotated by BRENDA team
biovar Microtus str. 91001
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
defects in human alpha-GAL lead to the development of Fabry disease, a lysosomal storage disorder characterized by the buildup of alpha-galactosylated substrates in the tissues
malfunction
-
dendritic cells deficient in alpha-galactosidase-A activity induce vigorous iNKT cell activation in the absence of exogenous antigen. Reconstitution of alpha-galactosidase-A-deficient dendritic cells with recombinant enzyme fully abrogate the iNKT cell response. Lack of alpha-galactosidase-A promote iNKT cell stimulation in vivo. Endogenous iNKT cells in Gla-/- hosts show signs of chronic exposure to self antigens
metabolism
-
stachyose biosynthesis
physiological function
-
AkalphaGal is a thylakoid membrane-degrading enzyme involved in the degradation of digalactosyl diacylglycerol during rice leaf senescence
physiological function
-
raffinose phloem unloading
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-azido-1-deoxy-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-, Q8A6L0
-
-
-
?
1-naphthyl alpha-D-galactoside + H2O
1-naphthol + D-glucose
show the reaction diagram
-
-
-
-
?
2,4-dinitrophenyl alpha-D-galactoside + H2O
2,4-dinitrophenol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
2,5-dinitrophenyl alpha-D-galactoside + H2O
2,5-dinitrophenol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
2-naphthyl alpha-D-galactoside + H2O
2-naphthol + D-glucose
show the reaction diagram
-
-
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + D-galactopyranose
show the reaction diagram
-, Q2XQ11
-
16.5% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside
-
?
2-nitrophenyl alpha-D-galactoside + H2O
2-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
2-nitrophenyl alpha-D-galactoside + H2O
2-nitrophenol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
3,4-dimethylphenyl alpha-D-galactoside + H2O
3,4-dimethylphenol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
3-nitrophenyl alpha-D-galactoside + H2O
3-nitrophenol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
3-nitrophenyl-alpha-D-galactopyranoside + H2O
3-nitrophenol + D-galactopyranose
show the reaction diagram
-, Q2XQ11
-
5.6% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside
-
?
4-bromophenyl alpha-D-galactoside + H2O
4-bromophenol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
4-methoxyphenyl alpha-D-galactoside + H2O
4-methoxyphenol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
show the reaction diagram
-
-
-
-
-
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
Thermomyces lanuginosus, Thermomyces lanuginosus IMI 158749
-
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
alpha-D-galactose + 4-methylumbelliferone
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-galactoside + H2O
4-methylumbelliferol + alpha-D-galactose
show the reaction diagram
-
-
-
-
-
4-methylumbelliferyl-alpha-D-galactoside + H2O
4-methylumbelliferol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-, Q19AX0
highest activity
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-, Q8A6L0
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
Tachigali multijuga
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
C7SEV1
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
Aspergillus niger M47, Penicillium chrysogenum LN33
-
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
-, Q1KTD9
-
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + alpha-D-xylopyranose
show the reaction diagram
-
9.64% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-, Q19AX0
low activity
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
Q7WWP9
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
activity measured in total fly homogenates, substrate concentration of 60 mM in citrate buffer
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-, Q2XQ11
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
Q9Z4N5, -
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-, Q9KAQ9
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
Gibberella sp.
C6FJG8
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
extracellular enzyme, intracellular enzyme
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
Debaryomyces hansenii UFV-1
-
extracellular enzyme, intracellular enzyme
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
C6FJG8
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranoside
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranoside
show the reaction diagram
Bispora sp.
-
-
-
-
?
4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
show the reaction diagram
-
-
-
-
?
4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
show the reaction diagram
Q9WXC1
-
-
-
?
4-O-[4-O[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]beta-D-mannopyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]beta-D-mannopyranosyl]-beta-D-mannose
show the reaction diagram
-
-
-
-
?
6(1)-alpha-D-galactosyl mannobiose + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
2.6% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannobiose + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
6.8% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannotriose + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
2.7% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannotriose + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
0.5% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
7.3% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6-bromo-2-naphthyl alpha-D-galactoside + H2O
6-bromo-2-naphthol + D-galactose
show the reaction diagram
-
-
-
-
?
6-bromo-2-naphthyl-alpha-D-galactopyranoside + H2O
6-bromo-2-naphthol + D-galactose
show the reaction diagram
Gibberella sp., Gibberella sp. F75
C6FJG8
-
-
-
?
alpha-D-galactopyranosyl-(1-6)-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
alpha-D-galactose + beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose
show the reaction diagram
Q9FXT4
-
-
-
?
arabinogalactan + H2O
?
show the reaction diagram
-
alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3
-
-
?
beta-naphthyl alpha-D-galactoside + H2O
beta-naphthol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
carob galactomannan + H2O
?
show the reaction diagram
-
-
-
-
?
cellobiose + phenyl alpha-D-galactoside
alpha-D-Gal-D-Glc-beta-D-Glc-alpha-D-Gal-beta-D-Glc-D-Glc + alpha-D-Gal-beta-D-Glc-D-Glc
show the reaction diagram
Coffea sp.
-
-
-
-
?
D-galactose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-galactose + phenyl alpha-D-galactoside
?
show the reaction diagram
Q6IYF5
-
-
-
?
D-galactose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-glucose + melibiose
melibiose + D-glucose
show the reaction diagram
-
-
-
-
?
D-glucose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-glucose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-glucose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-glucose + raffinose
?
show the reaction diagram
-
-
-
-
?
D-mannose + phenyl alpha-D-galactoside
epimelibiose + H2O
show the reaction diagram
-
-
-
-
?
D-sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
16.30% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
D-sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
35.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
D-sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Bifidobacterium animalis subsp. lactis LAFTI B94, Bifidobacterium longum LAFTI Bl536, Streptococcus thermophilus St1342
-
-
-
-
?
di-galactomannopentose + H2O
?
show the reaction diagram
-
-
-
-
?
digalactosyl diacylglycerol + H2O
?
show the reaction diagram
-
-
-
-
?
ethanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
ethyl alpha-D-galactoside + H2O
ethanol + D-galactose
show the reaction diagram
-
-
-
-
?
galactan + H2O
?
show the reaction diagram
-
alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3
-
-
?
galactinol + H2O
alpha-D-galactopyranose + myoinositol
show the reaction diagram
-
-
-
-
?
galactinol + H2O
alpha-D-galactopyranose + myoinositol
show the reaction diagram
-
-
-
-
?
galactinol + H2O
alpha-D-galactopyranose + myoinositol
show the reaction diagram
-
-
-
-
?
galactobisylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
galactomannan + H2O
?
show the reaction diagram
-
-
-
-
?
galactomannan + H2O
?
show the reaction diagram
Tachigali multijuga
-
-
-
-
?
galactomannan + H2O
?
show the reaction diagram
-
with galactose/mannose ratios of 2.4 and of 1.72
-
-
?
galactomannotriose + H2O
?
show the reaction diagram
-
-
-
-
?
gentianose + phenyl alpha-D-galactoside
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
gentibiose-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
1.28fold increased activity compared to gentibiose
-
-
?
gentiobiose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
gentiobiose + phenyl alpha-D-galactoside
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
globopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
globotriaosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
globotriaosylceramide + H2O
D-Galalpha(1,4)D-Glu-ceramide + D-galactose
show the reaction diagram
-
i.e. D-Galalpha(1,4)D-Galalpha(1,4)D-Glu-ceramide
-
-
?
globotriose + H2O
?
show the reaction diagram
-
-
-
-
?
globtriglycosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
glycerol + melibiose
floridoside + H2O
show the reaction diagram
Coffea sp.
-
-
-
-
?
glycerol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
Bispora sp.
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
guar gum + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
1.7% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
guar gum + H2O
?
show the reaction diagram
Talaromyces flavus CCF2686
-
-
-
-
?
isopentanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
isopropanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
lactose + H2O
beta-D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
lactose + H2O
beta-D-galactose + D-glucose
show the reaction diagram
-
enzyme P1: 6.2% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
lactose + H2O
beta-D-galactose + D-glucose
show the reaction diagram
-
2.3% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
lactose + phenyl alpha-D-galactoside
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
lactulose + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
Bispora sp.
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
locust bean gum + H2O
?
show the reaction diagram
Q7XIV4, Q9FXT4
1.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
locust bean gum + H2O
?
show the reaction diagram
Talaromyces flavus CCF2686
-
-
-
-
?
m-chlorophenyl alpha-D-galactoside + H2O
m-chlorophenol + D-galactose
show the reaction diagram
-
-
-
-
?
m-cresyl alpha-D-galactoside + H2O
m-cresol + D-galactose
show the reaction diagram
-
-
-
-
?
m-nitrophenyl alpha-D-galactoside + H2O
m-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
?
m-nitrophenyl alpha-D-galactoside + H2O
m-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
?
m-nitrophenyl alpha-D-galactoside + H2O
m-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
?
malitol + H2O
?
show the reaction diagram
-
-
-
-
?
malitol-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
2.66fold increased activity compared to malitol
-
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
-
-
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
enzyme P1: 4% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
10.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
18.12% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
6.07% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
maltose + phenyl alpha-D-galactoside
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
maltose-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
3.19fold increased activity compared to maltose
-
-
?
mannitol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Q6IYF5
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Coffea sp.
-
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Coffea sp.
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Coffea sp.
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-, Q2XQ11
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Rhizopus sp.
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Citrullus battich
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Q9Z4N5, -
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Q97U94, -
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Tachigali multijuga
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Gibberella sp.
C6FJG8
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-, Q19AX0
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
C7SEV1
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
7.3% of Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Q7XIV4, Q9FXT4
1.2% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
15.36% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
16.15% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Q7XIV4, Q9FXT4
6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
7.68% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
negligible hydrolytic activity towards melibiose
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
intracellular enzyme
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Rhizopus sp. F78
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Geobacillus stearothermophilus NCIM-5146
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Debaryomyces hansenii UFV-1
-
intracellular enzyme
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Hypocrea jecorina RUT C-30
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
C6FJG8
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Q97U94
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Talaromyces flavus CCF2686
-
negligible hydrolytic activity towards melibiose
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
Q9WXC1
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
42.1% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
35.5% of Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
enzyme P1: 6.9% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
Thermomyces lanuginosus IMI 158749
-
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
Bifidobacterium breve 203
-
-
-
-
?
melibiose + H2O
alpha-D-galactose + alpha-D-glucose
show the reaction diagram
Q9FXT4
-
-
-
?
melibiose + H2O
alpha-D-galactose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + alpha-D-glucose
show the reaction diagram
Thermotoga neapolitana, Thermotoga maritima, Thermotoga maritima DSMZ3109, Thermotoga neapolitana DSMZ5068
-
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
Bispora sp.
-
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + melibiose
?
show the reaction diagram
-
-
-
-
?
melibiose + melibiose
mannotriose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + methanol
methyl alpha-D-galactoside + D-glucose
show the reaction diagram
Coffea sp.
-
-
-
-
?
melibiose-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
1.45fold decreased activity compared to melibiose
-
-
?
meso-inositol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
methyl alpha-D-galactoside + H2O
methanol + D-galactose
show the reaction diagram
-
-
-
-
?
methyl alpha-D-galactoside + H2O
methanol + D-galactose
show the reaction diagram
-
-
-
-
?
methyl alpha-D-galactoside + H2O
methanol + D-galactose
show the reaction diagram
-
-
-
-
?
N-acetyl-D-galactosamine + melibiose
6-O-alpha-D-galactosyl-N-acetyl-D-galactosamine + D-glucose
show the reaction diagram
-
-
-
-
?
N-acetyl-D-glucosamine + melibiose
6-O-alpha-D-galactosyl-N-acetyl-D-glucosamine + H2O
show the reaction diagram
-
-
-
-
?
n-butanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
n-propanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
n-propyl alpha-D-galactoside + H2O
n-propanol + D-galactose
show the reaction diagram
-
-
-
-
?
O-beta-D-mannopyranosyl-(1-4)-O-[alpha-D-galactopyranosyl-(1-6)]-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
?
show the reaction diagram
Q9FXT4
-
-
-
?
o-cresyl alpha-D-galactoside + H2O
o-cresol + D-galactose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl alpha-D-fucopyranoside + H2O
o-nitrophenol + D-fucose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
27.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + alpha-D-galactose
show the reaction diagram
Thermotoga maritima, Thermotoga maritima DSMZ3109
-
-
-
-
?
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
-
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl beta-D-galactopyranoside
o-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
4.53% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
o-nitrophenyl-alpha-N-acetyl-galactosaminide + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranose + H2O
o-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
-
-
-
?
p-aminophenyl alpha-D-galactoside + H2O
p-aminophenol + D-galactose
show the reaction diagram
-
-
-
-
?
p-cresyl alpha-D-galactoside + H2O
p-cresol + D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-fucoside + H2O
p-nitrophenol + D-fucose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + D-glucose
?
show the reaction diagram
-
transglycosylation
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Q6IYF5
-
-
-
-
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Q9FXT4
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Rhizopus sp.
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Q9WXC1
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Absidia sp.
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Q97U94, -
-
-
-
-
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
100% activity
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Thermotoga maritima DSMZ3109
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Rhizopus sp. F78
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Thermomyces lanuginosus CBS 395.62/b
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Geobacillus stearothermophilus NCIM-5146
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Bifidobacterium animalis subsp. lactis LAFTI B94, Bifidobacterium longum LAFTI Bl536, Streptococcus thermophilus St1342
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Geobacillus stearothermophilus NCIM 5146
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Thermotoga neapolitana DSMZ5068
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Bifidobacterium breve 203
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Q97U94
-
-
-
-
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Absidia sp. WL511
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Talaromyces flavus CCF2686
-
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + D-galactose
show the reaction diagram
Q9SP05, -
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + D-galactose
show the reaction diagram
Solanum lycopersicum Mill.
Q9SP05
-
-
-
?
p-nitrophenyl alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Q7XIV4, Q9FXT4
100% activity
-
-
?
p-nitrophenyl alpha-D-glucopyranoside + H2O
p-nitrophenol + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl beta-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
enzyme P1: 5.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: no activity
-
-
?
p-nitrophenyl beta-L-arabinoside + H2O
p-nitrophenol + L-arabinose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
-
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
-
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
-
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Q9Z4N5, -
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Hypocrea jecorina RUT C-30
-
-
-
-
?
p-nitrophenyl-beta-D-galactopyranose + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-L-arabinopyranoside + H2O
p-nitrophenol + beta-L-arabinose
show the reaction diagram
Q97U94, -
-
-
-
?
phenyl alpha-D-galactoside + H2O
phenol + D-galactose
show the reaction diagram
-
-
-
-
?
phenyl alpha-D-galactoside + H2O
phenol + alpha-D-galactose
show the reaction diagram
O33835
-
-
-
-
phenyl alpha-D-galactoside + methanol
methyl alpha-D-galactoside + phenol
show the reaction diagram
-
-
-
-
?
planteose + H2O
?
show the reaction diagram
-
-
-
-
?
raffinose + galactinol
stachyose + H2O
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Prunus dulcis, Coffea sp.
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Coffea sp.
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Citrullus battich
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Q9Z4N5, -
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-, Q9KAQ9
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Tachigali multijuga
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Gibberella sp.
C6FJG8
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
30.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
60.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
78.57% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
intracellular enzyme
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Thermomyces lanuginosus CBS 395.62/b
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Geobacillus stearothermophilus NCIM-5146
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Bifidobacterium animalis subsp. lactis LAFTI B94, Bifidobacterium longum LAFTI Bl536, Streptococcus thermophilus St1342
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Debaryomyces hansenii UFV-1
-
intracellular enzyme
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Hypocrea jecorina RUT C-30
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Thermotoga neapolitana DSMZ5068
-
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
C6FJG8
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
-
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Q9FXT4
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
-
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Q9WXC1
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Q97U94, -
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
C7SEV1
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Bispora sp.
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
52.4% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
20% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
enzyme P1: 11.8% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 54.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
Vmax is 1.8fold higher than that with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Q7XIV4, Q9FXT4
1.89% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Q7XIV4, Q9FXT4
10.1% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Thermomyces lanuginosus IMI 158749
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Thermotoga maritima DSMZ3109
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Bifidobacterium breve 203
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Q97U94
-
-
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
Rhizopus sp.
-
-
-
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
-
assay at pH 7.5, 37C, 15 min, reaction terminated by 2 min boiling
-
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
-
assay at pH 7.5, 37C, 20 min, reaction terminated by keeping the reaction mixture in boiling water for 2 min
-
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
Rhizopus sp. F78
-
-
-
-
?
raffinose + melibiose
stachyose + H2O
show the reaction diagram
-
-
-
-
?
raffinose + methanol
methyl alpha-D-galactoside + verbascose + ajugose
show the reaction diagram
Coffea sp.
-
-
-
-
?
raffinose + raffinose
?
show the reaction diagram
-
-
-
-
?
raffinose-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
1.67fold increased activity compared to raffinose
-
-
?
sorbitol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Q9FXT4
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Rhizopus sp.
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Q9WXC1
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Q97U94, -
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
C7SEV1
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Bispora sp.
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
69.7% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
17.8% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
20fold less active than with raffinose
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
enzyme P1: 5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
high actvity with alpha-Gal I, low activity with alpha-Gal II
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
Vmax is 2fold higher than that with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Q7XIV4, Q9FXT4
0.47% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Q7XIV4, Q9FXT4
1.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
11.74% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
78.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
85.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Thermotoga maritima DSMZ3109
-
20fold less active than with raffinose
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Rhizopus sp. F78
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Thermomyces lanuginosus CBS 395.62/b
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Geobacillus stearothermophilus NCIM-5146
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Bifidobacterium animalis subsp. lactis LAFTI B94, Bifidobacterium longum LAFTI Bl536, Streptococcus thermophilus St1342
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Thermotoga neapolitana DSMZ5068
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Bifidobacterium breve 203
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Q97U94
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
-
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Citrullus battich
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Q9Z4N5, -
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Tachigali multijuga
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Gibberella sp.
C6FJG8
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Debaryomyces hansenii, Debaryomyces hansenii UFV-1
-
intracellular enzyme
-
-
?
stachyose + H2O
?
show the reaction diagram
Hypocrea jecorina RUT C-30
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
C6FJG8
-
-
-
?
stachyose + H2O
raffinose + D-galactose
show the reaction diagram
Q9Z4N5, -
-
-
-
?
stachyose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
stachyose + methanol
methyl alpha-D-galactoside + verbascose + ajugose
show the reaction diagram
Coffea sp.
-
-
-
-
?
stachyose + stachyose
?
show the reaction diagram
-
-
-
-
?
sucrose + galactinol
raffinose + H2O
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
sucrose + melibiose
raffinose + stachyose + planteose
show the reaction diagram
Coffea sp.
-
-
-
-
?
sucrose + melibiose
raffinose + D-glucose
show the reaction diagram
-
-
-
-
?
sucrose + phenyl alpha-D-galactoside
raffinose + stachyose + planteose
show the reaction diagram
Coffea sp.
-
-
-
-
?
sucrose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
sucrose + raffinose
planteose + D-glucose
show the reaction diagram
-
-
-
-
?
trehalose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
trihexosylceramide + H2O
?
show the reaction diagram
-
-
-
-
-, ?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
show the reaction diagram
-
-
-
-
?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
show the reaction diagram
-
-
-
-
?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
show the reaction diagram
-
20fold less active than with raffinose
-
-
?
methyl alpha-D-galactoside + H2O
methanol + D-galactose
show the reaction diagram
Citrullus battich
-
-
-
-
?
additional information
?
-
-
Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A
-
-
-
additional information
?
-
-
Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A. This enzyme is responsible for the hydrolysis of terminal alpha-galactosidase linkages in various glycolipids
-
-
-
additional information
?
-
-
raffinose induces about twice the activity than melibiose
-
-
-
additional information
?
-
-, Q9L905
transcription is induced by melibiose but is not totally repressed by glucose
-
-
-
additional information
?
-
-
activity with soy molasses
-
-
-
additional information
?
-
-
slight preference for branched galactosyl residues over straight chain residues
-
-
-
additional information
?
-
-
alpha-galactosidase 2 has the ability to markedly catalyze increased pectin solubility and depolymerization while the polymers are still structurally attached to the cell walls mimicking, in part, the changes that occur during ripening. The enzyme might contribute to papaya fruit softening during ripening
-
-
-
additional information
?
-
P06280
Fabry disease is an X-linked genetic disorder resulting from a deficiency of alpha-galactosidase activity
-
-
-
additional information
?
-
-
one of the principal enzymes involved in the modification or degradation of plant cell wall galactomannans
-
-
-
additional information
?
-
Q5DUH7
one of the principal enzymes involved in the modification or degradation of plant cell wall galactomannans
-
-
-
additional information
?
-
-
activity is not enhanced on sucrose and beta-linked carbohydrates and fructooligosaccharides
-
-
-
additional information
?
-
-
D-lactose, o-nitrophenyl beta-D-galactopyranoside, p-nitrophenyl alpha-D-glucopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates
-
-
-
additional information
?
-
-
D-sucrose, p-nitrophenyl alpha-D-glucopyranoside, p-nitrophenyl alpha-D-xylulopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates
-
-
-
additional information
?
-
-
lactose, o-nitrophenyl beta-D-galactopyranoside, p-nitrophenyl alpha-D-xylulopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates
-
-
-
additional information
?
-
Rhizopus sp.
-
no detectable activity towards guar gum
-
-
-
additional information
?
-
-
no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal
-
-
-
additional information
?
-
O33835
no detectable enzymatic activity on xylo-, fuco-, manno-, or glucopyranosides
-
-
-
additional information
?
-
Q97U94, -
shows no activity toward p-nitrophenyl-alpha-substituted hexoses such as D-glucose, D-mannose, L-rhamnose, or N-acetyl-D-galactosaminide, p-nitrophenyl-beta-substituted hexoses such as D-galactose, D-glucose, and D-mannose do not support catalysis, nor does the pentose p-nitrophenyl-beta-D-xylopyranoside
-
-
-
additional information
?
-
-, Q2XQ11
enzyme additionally catalyzes transglycosylation. It is able to synthesize a trisaccharide galactose-(alpha-1,4)-galactose(alpha-1,6)-glucose using melibiose as substrate. In presence of 4-nitrophenyl-alpha-D-galactopyranoside, enzyme catalyzes glycosyl transfer to various acceptors
-
-
-
additional information
?
-
-, Q2XQ11
no substrate: 3-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galacturonide, 2-nitrophenyl-alpha-D-glucopyranoside, 2-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside, and 2-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside
-
-
-
additional information
?
-
-, Q9KAQ9
no substrate: galactomannan
-
-
-
additional information
?
-
-, Q1KTD9
under optimum pH conditions and high melibiose concentration of 40% w/v, the enzyme is able to form oligosaccharides with degree of polymerisation of 3 and more at higher concentration than with degree of polymerization 2, with a total yield of 20.5% w/w
-
-
-
additional information
?
-
-
when treated with the Bacillus megaterium VHM1 enzyme, the flatulence-causing sugars in soymilk are completely hydrolyzed within 1.5 h
-
-
-
additional information
?
-
Rhizopus sp. F78
-
no detectable activity towards guar gum
-
-
-
additional information
?
-
Geobacillus stearothermophilus NCIM-5146
-
no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal
-
-
-
additional information
?
-
Thermotoga neapolitana DSMZ5068
-
slight preference for branched galactosyl residues over straight chain residues
-
-
-
additional information
?
-
Bifidobacterium breve 203
-
raffinose induces about twice the activity than melibiose
-
-
-
additional information
?
-
Q97U94
shows no activity toward p-nitrophenyl-alpha-substituted hexoses such as D-glucose, D-mannose, L-rhamnose, or N-acetyl-D-galactosaminide, p-nitrophenyl-beta-substituted hexoses such as D-galactose, D-glucose, and D-mannose do not support catalysis, nor does the pentose p-nitrophenyl-beta-D-xylopyranoside
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
globotriaosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
globotriaosylceramide + H2O
D-Galalpha(1,4)D-Glu-ceramide + D-galactose
show the reaction diagram
-
i.e. D-Galalpha(1,4)D-Galalpha(1,4)D-Glu-ceramide
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-, Q19AX0
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
42.1% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
27.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + D-glucose
?
show the reaction diagram
-
transglycosylation
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
52.4% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
69.7% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
trihexosylceramide + H2O
?
show the reaction diagram
-
-
-
-
-
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A
-
-
-
additional information
?
-
-
Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A. This enzyme is responsible for the hydrolysis of terminal alpha-galactosidase linkages in various glycolipids
-
-
-
additional information
?
-
-
raffinose induces about twice the activity than melibiose
-
-
-
additional information
?
-
-, Q9L905
transcription is induced by melibiose but is not totally repressed by glucose
-
-
-
additional information
?
-
-
alpha-galactosidase 2 has the ability to markedly catalyze increased pectin solubility and depolymerization while the polymers are still structurally attached to the cell walls mimicking, in part, the changes that occur during ripening. The enzyme might contribute to papaya fruit softening during ripening
-
-
-
additional information
?
-
P06280
Fabry disease is an X-linked genetic disorder resulting from a deficiency of alpha-galactosidase activity
-
-
-
additional information
?
-
-
one of the principal enzymes involved in the modification or degradation of plant cell wall galactomannans
-
-
-
additional information
?
-
Q5DUH7
one of the principal enzymes involved in the modification or degradation of plant cell wall galactomannans
-
-
-
additional information
?
-
-
when treated with the Bacillus megaterium VHM1 enzyme, the flatulence-causing sugars in soymilk are completely hydrolyzed within 1.5 h
-
-
-
additional information
?
-
Bifidobacterium breve 203
-
raffinose induces about twice the activity than melibiose
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NAD+
-
required for enzymatic activity
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ag+
-
inhibits enzymatic activity
Ag2+
-
strong inhibitory effect
Ca2+
-
109.9% activity at 10 mM
Ca2+
-
slight inhibitory effect
Co2+
-, Q9KAQ9
strong activation
Cr3+
C7SEV1
the activity of recombinant Aga-F78 is significantly enhanced in the presence of Cr3+
Cu2+
-
1 mM, 1.2fold stimulation
Cu2+
-
inhibits enzymatic activity
Cu2+
Bispora sp.
-
moderate stimulation
Cu2+
-
strong inhibitory effect
EDTA
-
activity enhanced by 2fold
Fe2+
-, Q9KAQ9
strong activation
Fe3+
C7SEV1
the activity of recombinant Aga-F78 is significantly enhanced in the presence of Fe3+
Hg2+
-
inhibits enzymatic activity
Hg2+
-
strong inhibitory effect
K+
-
activates the enzyme
K+
-
activates at 10 mM
K+
Rhizopus sp.
-
9.5% increased activity at 1 mM
K+
-
105.7% activity at 10 mM
Mg2+
-
activates at 10 mM
Mg2+
-
105.6% activity at 10 mM
Mn2+
-
activates at 10 mM
Mn2+
-, Q9KAQ9
strong activation
Mn2+
-
activity enhanced by 2fold
Na+
-
124.8% activity at 10 mM
Ni2+
-, Q9KAQ9
moderate activation
Ni2+
Bispora sp.
-
moderate stimulation
Ni2+
-
slight inhibitory effect
Pb2+
C7SEV1
the activity of recombinant Aga-F78 is significantly enhanced in the presence of Pb2+
SDS
-
inhibits enzymatic activity
Zn2+
-, Q9KAQ9
moderate activation
Zn2+
-
activity enhanced by 2fold
Zn2+
Bispora sp.
-
moderate stimulation
Zn2+
-
slight inhibitory effect
Mn2+
-
required for enzymatic activity
additional information
Q7XIV4, Q9FXT4
Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity; Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity
additional information
-
Ba2+, Ca2+, Co2+, Fe3+, K+, Mg2+, Mn2+, Na+, Ni2+, Zn2+ (1 mM) and EDTA (10 mM) have no influence on enzyme activity
additional information
C7SEV1
the addition of other metal ions or chemicals has little or no effect on the activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(2S,3R,4R,5S)-2-(hydroxymethyl)-5-[(methylamino)methyl]pyrrolidine-3,4-diol
-
-
(2S,3R,4R,5S)-2-[(ethylamino)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol
-
-
1-deoxy-L-altronojirimycin hydrochloride
-
1000fold less effective than 1-deoxygalactonojirimycin
1-deoxygalactonojirimycin
-
IC50: 63 nM with mutant enzyme S65T, 48 nM with mutant enzyme S65A, 58 nM with mutant enzyme S65D and 59 nM with wild-type enzyme
1-deoxygalactonojirimycin
-
binding to the enzyme protein is enthalpy-driven and stronger than binding of galactostatin bisulfite. Inhibitor fits to the active-site pocket and undergoes hydrogen bonding with residues comprising the active-site pocket including the catalytic ones
1-deoxygalactonojirimycin
-
-
1-deoxygalactonojirimycin
-
-
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
31% residual activity at 100 mM, at pH 6.0
1-ethyl-3-(dimethylaminopropyl) carbodiimide
-
inactivation by 1-ethyl-3-(dimethylaminopropyl) carbodiimide plus glycine ethyl ester follows a two-phase kinetics: a fast one decreases in presence of melibiose and another slow not affected by melibiose
2,4,6-Trinitrobenzenesulfonic acid
-
20% residual activity at 2 mM, at pH 8.4
2-mercaptoethanol
-
98.66% activity at 10 mM
2-mercaptoethanol
-
95% activity at 10 mM
Ag+
Citrullus battich
-
-
Ag+
Q9WXC1
1 mM, about 80% inhibition
Ag+
-
1 mM, 68% inhibition
Ag+
Q7XIV4, Q9FXT4
alpha-Gal II, 67% decrease of activity at 1 mM; alpha-Gal III, 84% decrease of activity at 1 mM
Ag+
Rhizopus sp.
-
no activity at 1 mM
Ag+
-
strong inhibitor
Ag+
-
inactivation
Ag+
-, Q2XQ11
strong
Ag+
-
inactivation, intracellular enzyme
Ag+
Tachigali multijuga
-
-
Ag+
C7SEV1
strong inhibitor
Ag+
-
inhibits enzymatic activity
Ag+
-
completely inhibits activity
Ag2+
Q9FXT4
1 mM, 46% inhibition
Ag2+
Q97U94, -
complete inhibition at 10 mM
Ag2+
Bispora sp.
-
strongly inhibits AgalB activity
AgNO3
-
1.0 mM, complete inhibition, alpha-galactosidase 1; 1.0 mM, complete inhibition, alpha-galactosidase 2; 1.0 mM, complete inhibition, alpha-galactosidase 3
Al3+
Rhizopus sp.
-
66.7% activity at 1 mM
alpha-D-galactopyranosyl azide
-
75% inhibition at 2 mM
alpha-D-Galactose
-
2 mM, 17% inhibition of enzyme P1, 68.5% inhibition of enzyme P2
beta-D-galactose
Q92456
competitive
Ca2+
-
strong inhibition at 10 mM
Ca2+
Q6IYF5
90.4% residual activity at 1 mM
Ca2+
Rhizopus sp.
-
82.2% activity at 1 mM
Ca2+
-
96.25% activity at 10 mM
Ca2+
-
94% activity at 10 mM
Ca2+
Q97U94, -
complete inhibition at 10 mM
CaCl2
-
10 mM, 19.9% inhibition
Cd2+
-
alpha-galactosidase II
Cd2+
Q6IYF5
86.0% residual activity at 1 mM
Cd2+
Q97U94, -
complete inhibition at 10 mM
CdSO4
-
0.25 mM, 99.9% inhibition
citraconic anhydride
-
30% residual activity at 5 mM, at pH 7.8
Co2+
-
alpha-galactosidase II
Co2+
-
slight inhibition at 10 mM
Co2+
Rhizopus sp.
-
96.6% activity at 1 mM
Co2+
Q97U94, -
complete inhibition at 10 mM
CoSO4
-
0.25 mM, 8.3% inhibition
Cr3+
Rhizopus sp.
-
63.9% activity at 1 mM
Cr3+
-
partial inhibition
Cu2+
-
1 mM, strong inhibition
Cu2+
-
slight inhibition at 10 mM
Cu2+
Q6IYF5
76.8% residual activity at 1 mM
Cu2+
Rhizopus sp.
-
78.1% activity at 1 mM
Cu2+
-
complete inhibition at 10 mM
Cu2+
-
59.16% activity at 10 mM
Cu2+
-
11% activity at 10 mM
Cu2+
Q97U94, -
complete inhibition at 10 mM
Cu2+
Gibberella sp.
C6FJG8
strong
Cu2+
-, Q2XQ11
strong
Cu2+
-
inactivation, intracellular enzyme
Cu2+
Tachigali multijuga
-
-
Cu2+
-
inhibits enzymatic activity
CuCl2
-
1.0 mM, 30.8% inhibition
CuCl2
-
1 mM, 50% inhibition
CuSO4
-
2 mM, 94.5% inhibition of enzyme P1, 48% inhibition of enzyme P2
D-fucose
-
75 mM, 11.1% inhibition, alpha-galactosidase 3; 75 mM, 25.5% inhibition, alpha-galactosidase 2; 75 mM, 47.1% inhibition, alpha-galactosidase 1
D-galactose
-
-
D-galactose
-
-
D-galactose
-
-
D-galactose
-
-
D-galactose
-
10 mM, complete inhibition
D-galactose
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
D-galactose
-
10 mM, 81.2% inhibition, alpha-galactosidase 1; 5 mM, 63% inhibition, alpha-galactosidase 2; 5 mM, 66.6% inhibition, alpha-galactosidase 3
D-galactose
-
inhibitory effect at 10 mM
D-galactose
-
51% inhibition at 2 mM
D-galactose
-
competitive, intracellular enzyme
D-galactose
Tachigali multijuga
-
competitive
D-galactose
-
inhibits enzymatic activity
D-glucose
-
-
D-glucose
-
94.4% activity at 10 mM
D-glucose
-
96% activity at 10 mM
D-lactose
-
89.51% activity at 10 mM
D-lactose
-
88% activity at 10 mM
D-maltose
-
92.8% activity at 10 mM
D-maltose
-
91.33% activity at 10 mM
D-maltose
-
94% activity at 10 mM
D-mannose
-
-
D-mannose
-
90.5% activity at 10 mM
D-mannose
-
90.05% activity at 10 mM
D-mannose
-
94% activity at 10 mM
D-sucrose
-
98.72% activity at 10 mM
D-xylose
-
10 mM, 12% inhibition
D-xylose
-
75 mM, 45% inhibition, alpha-galactosidase 3; 75 mM, 62.7% inhibition, alpha-galactosidase 2; 75 mM, 81.5% inhibition, alpha-galactosidase 1
D-xylose
-
49% inhibition at 2 mM
diethyldicarbonate
-
complete inhibition at 2 mM, at pH 6.0
EDTA
Rhizopus sp.
-
98.1% activity at 1 mM
EDTA
-
97.21% activity at 10 mM
Fe2+
Rhizopus sp.
-
75.1% activity at 1 mM
-
Fe2+
-
partial inhibition
-
Fe3+
Gibberella sp.
C6FJG8
strong
-
Fe3+
Bispora sp.
-
5 mM Fe3+ and Mn2+ decrease enzyme activity to 70% and 89% of its initial activity
-
FeCl2
-
1 mM, 50% inhibition
FeCl3
-
1.0 mM, 46.2% inhibition
FeSO4
-
1.0 mM, 50.7% inhibition
galactose
-
powerful inhibitor of alpha-Gal II, alpha-Gal III and alpha-Gal IV. 30% inhibition at 0.5 mM, nearly 100% inhibition at 40 mM
galactose
-
-
galactose
-
relative inhibition of 83.8%, effective inhibitor
galactostatin bisulfite
-
inhibits in vitro, increases enzyme activity in cultured Fabry fibroblasts. Binding is less strong than binding of 1-deoxygalactonojirimycin, and interaction with enzyme protein is mainly enthalpie-driven but entropy-driven factors may be involved. Inhibitor fits to the active-site pocket and undergoes hydrogen bonding with residues comprising the active-site pocket including the catalytic ones
galactostatin bisulfite
-
-
gamma-1,4-galactonolactone
-
-
-
glycerol 3-phosphate
-
-
Hg+
-
complete inactivation at 10 mM
Hg+
Q6IYF5
20.9% residual activity at 1 mM
Hg2+
Citrullus battich
-
-
Hg2+
Q9WXC1
1 mM, more than 95% inhibition
Hg2+
-
1 mM, 91% inhibition
Hg2+
-
1 mM, strong inhibition
Hg2+
Q9FXT4
1 mM, 98% inhibition
Hg2+
Q7XIV4, Q9FXT4
alpha-Gal II, 93% decrease of activity at 1 mM; alpha-Gal III, 87% decrease of activity at 1 mM
Hg2+
Rhizopus sp.
-
no activity at 1 mM
Hg2+
-
strong inhibitor
Hg2+
Q97U94, -
complete inhibition at 10 mM
Hg2+
Gibberella sp.
C6FJG8
strong
Hg2+
-
inactivation
Hg2+
-, Q2XQ11
strong
Hg2+
Tachigali multijuga
-
-
Hg2+
C7SEV1
strong inhibitor
Hg2+
-
inhibits enzymatic activity
Hg2+
-
completely inhibits activity
Hg2+
Bispora sp.
-
strongly inhibits AgalB activity
HgCl2
-
1.0 mM, 88.2% inhibition
HgCl2
-
0.5 mM, complete inhibition, alpha-galactosidase 1; 2.0 mM, complete inhibition, alpha-galactosidase 2; 2.0 mM, complete inhibition, alpha-galactosidase 3
iodoacetamide
-
95.88% activity at 10 mM
iodoacetamide
-
98.87% activity at 10 mM
iodoacetic acid
-
-
K+
-
96.48% activity at 10 mM
KCl
-
2.0 M, 41.3% inhibition; 2.0 M, 74.6% inhibition, alpha-galactosidase 3; 2.0 M, 8.8% inhibition, alpha-galactosidase 1
L-arabinose
-
-
L-ascorbic acid
-
-
lactose
-
12% inhibition at 2 mM
Li+
Q6IYF5
93.3% residual activity at 1 mM
Li+
Rhizopus sp.
-
96.7% activity at 1 mM
Mannose
-
70% inhibition of alpha-Gal I at 40 mM
melibiose
-
with p-nitrophenyl alpha-D-galactopyranoside as substrate
melibiose
-
2 mM, 16% inhibition of enzyme P1, 36% inhibition of enzyme P2
melibiose
-
39.7% activity at 10 mM
melibiose
-
74.14% activity at 10 mM
melibiose
-
58% activity at 10 mM
melibiose
-
46% inhibition at 2 mM
melibiose
Tachigali multijuga
-
-
methyl alpha-D-galactopyranoside
-
15% inhibition at 2 mM
methyl beta-D-galactopyranoside
-
13% inhibition at 2 mM
Mg2+
Q6IYF5
92.6% residual activity at 1 mM
Mg2+
Rhizopus sp.
-
98.2% activity at 1 mM
Mg2+
-
94.15% activity at 10 mM
Mg2+
-
98% activity at 10 mM
MgCl2
-
10 mM, 16.4% inhibition
Mn2+
Q6IYF5
91.5% residual activity at 1 mM
Mn2+
Rhizopus sp.
-
49.5% activity at 1 mM
Mn2+
Q97U94, -
complete inhibition at 10 mM
Mn2+
C7SEV1
strong inhibitor
Mn2+
Bispora sp.
-
5 mM Fe3+ and Mn2+ decrease enzyme activity to 70% and 89% of its initial activity
myo-Inositol
-
-
myo-Inositol
-
-
myo-Inositol
-
-
myo-Inositol
-
-
myo-Inositol
-
-
N-(N-benzyloxycarbonyl-epsilon-aminocaproyl)-alpha-D-galactopyranoside
Coffea sp.
-
-
N-Acetyl-D-galactosamine
-
-
N-bromosuccinimide
-
complete inhibition at 0.02 mM, at pH 5.0
N-bromosuccinimide
-
almost complete inhibition
N-bromosuccinimide
-
100% inhibition
n-butyldeoxygalactonojirimycin
-
2000fold less effective than 1-deoxygalactonojirimycin
N-epsilon-Aminocaproyl-alpha-D-galactopyranoside
Coffea sp.
-
-
Na+
-
94.2% activity at 10 mM
Na+
-
96% activity at 10 mM
NaCl
-
2.0 M, 21.9% inhibition, alpha-galactosidase 1; 2.0 M, 46.7% inhibition, alpha-galactosidase 2; 2.0 M, 78.9% inhibition, alpha-galactosidase 2
NEM
-
10 mM, 12.9% inhibition
Ni(2+)
Rhizopus sp.
-
92.7% activity at 1 mM
Ni2+
-
slight inhibition at 10 mM
Ni2+
Q97U94, -
complete inhibition at 10 mM
NiCl2
-
1.0 mM, 21.7% inhibition
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
Q7XIV4, Q9FXT4
alpha-Gal III shows less than 8% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20C for 1 h; alpha-Gal II shows less than 6% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20C for 1 h
p-chloromercuribenzoate
-
16% residual activity at 0.01 mM, at pH 5.8
p-hydroxymercuribenzoate
-
0.25 mM, 97.8% inhibition
p-nitrophenyl-alpha-D-galactoside
-
substrate inhibition above 4 mM, alpha-galactosidase 2; substrate inhibition above 4 mM, alpha-galactosidase 3
PCMB
Q9WXC1
1 mM, 30C, 30 min, complete inhibition of wild-type enzyme, no inhibition of mutant enzyme C159A
PCMB
Q9FXT4
1 mM, 98% inhibition
raffinose
-
with p-nitrophenyl alpha-D-galactopyranoside as substrate
raffinose
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
raffinose
-
80.60% activity at 10 mM
raffinose
-
81.73% activity at 10 mM
raffinose
-
93% activity at 10 mM
SDS
-
1 mM, 98% inhibition of enzyme P1, 37% inhibition of enzyme P2
SDS
Rhizopus sp.
-
no activity at 1 mM
SDS
-
46.9% activity at 10 mM
SDS
-
49.36% activity at 10 mM
SDS
-
complete inhibition at 10 mM
SDS
C7SEV1
strong inhibitor
SDS
-
completely inhibits activity
SDS
Bispora sp.
-
strongly inhibits AgalB activity
Sodium dodecyl sulfate
Gibberella sp.
C6FJG8
strong
Sodium dodecyl sulfate
Tachigali multijuga
-
-
sodium taurocholate
-
-
stachyose
-
with p-nitrophenyl alpha-D-galactopyranoside as substrate
stachyose
-
95.73% activity at 10 mM
stachyose
-
87.69% activity at 10 mM
stachyose
-
88% activity at 10 mM
Woodward's reagent K
-
21% residual activity at 5 mM, at pH 6.0
Zn2+
-
alpha-galactosidase II
Zn2+
-
strong inhibition at 10 mM
Zn2+
Q6IYF5
78.3% residual activity at 1 mM
Zn2+
Q97U94, -
complete inhibition at 10 mM
ZnCl2
-
1.0 mM, 99.3% inhibition
MnCl2
-
1.0 mM, 18.4% inhibition
additional information
Rhizopus sp.
-
not inhibited by Na+ and Zn2+
-
additional information
-
inhibiting saccharides used at three concentrations, 10, 50, and 100 mM, no inhibitory effects of glucose, inositol, sucrose
-
additional information
-
not inhibited by D-lactose
-
additional information
-
not inhibited by sucrose, EDTA, and K+
-
additional information
Q97U94, -
D-galactose, L-arabinose, D-fucose, sucrose, EDTA, dithiothreitol, Mg2+ and Mo2+ have no inhibitory effect
-
additional information
-
not inhibited by 2-acetamido-2-deoxy-D-galactopyranose, D-galactosamine hydrochloride, D-glucose, 2-deoxy-D-glucose, 6-deoxy-D-glucose, 2-acetamido-2-deoxy-D-glucopyranose, D-glucosamine hydrochloride, saccharose, stachyose, D-fructose and D-arabinose
-
additional information
-
beta-mercaptoethanol (1.0 mM) and urea (1.0 M) do not inhibit the enzyme activity
-
additional information
-
not inhibitory: Mg2+, Ni2+, Ca2+, Co2+, Mn2+
-
additional information
Gibberella sp.
C6FJG8
enzyme is strongly resistant to alpha-chymotrypsin, subtilisin A, and collagenase digestion
-
additional information
-
decrease of enzyme activity in mesocarp tissue during fruit development
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-deoxygalactonojirimycin
-
3.8fold increase at 0.02 mM after 5 days
1-deoxygalactonojirimycin
-
acts as a chemical chaperone and enhances catalytic activity and protein expression in milder mutations associated with the atypical form of Fabry disease
1-deoxygalactonojirimycin
-
T-cells from normal controls resond with 28% increase in alpha-galactosidase activity to treatment with 1-deoxygalactonojirimycin. In cells from patients with Fabry disease, response depends on he mutantion and ranges from no increase to fully normal activity. In normal cells and in cells from patients responding more than 25%, an increase in the mature lysosomal form of enzyme is observed after treatment. In the group of intermediate respoders with increases of 7-25% in activity upon treatment, an increase in protein stain but incomplete processing of the enzyme to the mature form is detected
2-mercaptoethanol
-
107.8% activity at 10 mM
2-mercaptoethanol
-
required for enzymatic activity
6-deoxy-D-glucose
-
the enzyme is produced exclusively in the presence of the specific inducer 6-deoxy-D-glucose, also named quinovose
beta-mercaptoethanol
-
activity enhanced by 2fold
D-glucose
Candida javanica
-
increase of activity
D-glucose
-
105.1% activity at 10 mM
D-sucrose
-
116.5% activity at 10 mM
dithiothreitol
-, Q9KAQ9
or similar reducing agent, required
EDTA
-
105% activity at 10 mM
EDTA
-, Q2XQ11
16% increase in activity
EDTA
C7SEV1
the activity of recombinant Aga-F78 is significantly enhanced in the presence of EDTA
iodoacetamide
-
104.3% activity at 10 mM
L-arabinose
-
the enzyme is strongly induced by L-arabinose
NAD+
-, Q9KAQ9
strong activation
NADP+
-, Q9KAQ9
slight activation
NH4+
-, Q2XQ11
23% increase in activity
Pb2+
Gibberella sp.
C6FJG8
5 mM, significant enhancement of activity
microwave
O33835
microwave irradiation (300 W) significantly stimulates activity
-
additional information
-
D-galactose, melibiose, raffinose, stachyose, methyl alpha-D-galactopyranoside, 6-deoxy-D-galactose, D-glucose, 2-deoxy D-glucose, 6-O-tosyl-D-glucose, 6-chloro-6-deoxy-dglucose, D-arabinose, and D-xylose do not stimulate enzyme production
-
additional information
-
increase of enzyme activity in leaves during fruit development at day 4 after anthesis
-
additional information
Bispora sp.
-
a synergistic (3fold) increase in guar gum hydrolysis is observed when beta-mannanase Man5A from Bispora sp. MEY-1 and r-AgalB are combined
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.037
-
2,4-dinitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.135
-
2,4-dinitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.143
-
2,5-dinitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.243
-
2,5-dinitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.133
-
2-nitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
-
0.143
-
2-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
-
0.28
-
2-nitrophenyl alpha-D-galactoside
-
pH 5.0, 65C
-
0.086
-
3,4-dimethylphenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.089
-
3,4-dimethylphenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.096
-
3-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.14
-
3-nitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.104
-
4-bromophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.111
-
4-bromophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
2.9
-
4-methylumbelliferyl alpha-D-galactopyranoside
-
alpha-galactosidase A
2.9
-
4-methylumbelliferyl alpha-D-galactopyranoside
-
-
6.8
-
4-methylumbelliferyl alpha-D-galactopyranoside
-
alpha-galactosidase B
6.8
-
4-methylumbelliferyl alpha-D-galactopyranoside
-
-
0.45
-
4-methylumbelliferyl alpha-D-galactoside
-
-
-
0.5
-
4-methylumbelliferyl alpha-D-galactoside
-
50C, pH 4.5
-
2.2
-
4-methylumbelliferyl alpha-D-galactoside
-
wild-type enzyme
-
2.3
-
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, mutant enzyme E66D; pH 4.6, wild-type enzyme
-
2.8
-
4-methylumbelliferyl alpha-D-galactoside
-
mutant enzyme Q279E and R301Q
-
3.4
-
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, mutant enzyme S65A
-
3.5
-
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, mutant enzyme S65T
-
4
-
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, 37C, mutant M51I
-
4.5
-
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, 37C, wild-type
-
1.8
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme Q279E, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
1.9
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme R301Q, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme R356W, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2.4
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme M296V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2.6
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme L166V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2.7
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme M296I, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2.8
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme A20P, in 0.1 M sodium citrate buffer (pH 4.6) at 37C; mutant enzyme N215S, in 0.1 M sodium citrate buffer (pH 4.6) at 37C; wild type enzyme, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.1
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme E66Q, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.2
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme M72V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.4
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme A156V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C; mutant enzyme I91T, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.6
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme F113L, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.7
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme A97V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
5.8
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme R112H, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
16.3
-
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme E59K, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
0.48
-
4-nitrophenyl alpha-D-galactopyranoside
-
pH 5.0, 40C
0.61
-
4-nitrophenyl alpha-D-galactopyranoside
-
pH 5.0, 40C
0.08
-
4-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.083
-
4-nitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.1
-
4-nitrophenyl alpha-D-galactoside
-
pH 5.0, 65C
0.19
-
4-nitrophenyl alpha-D-galactoside
-, Q8A6L0
mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
0.31
-
4-nitrophenyl alpha-D-galactoside
-, Q8A6L0
wild type enzyme, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
0.32
-
4-nitrophenyl alpha-D-galactoside
-, Q8A6L0
mutant enzyme E470Q, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
0.45
-
4-nitrophenyl alpha-D-galactoside
Tachigali multijuga
-
pH 5.0, 40C
0.51
-
4-nitrophenyl alpha-D-galactoside
-
-
0.2
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 40C
0.24
-
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant Q251W, pH 7.4, 30C
0.27
-
4-nitrophenyl-alpha-D-galactopyranoside
-, Q2XQ11
-
0.27
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 37C
0.29
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 4.5, 50C
0.32
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5.0, 55C
0.65
-
4-nitrophenyl-alpha-D-galactopyranoside
Q9Z4N5, -
pH 6.0, 36C
0.66
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 40C
0.72
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 40C
1.06
-
4-nitrophenyl-alpha-D-galactopyranoside
Gibberella sp.
C6FJG8
37C
1.4
-
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant Q251A, pH 7.4, 30C
2.47
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5.5, 45C
3.1
-
4-nitrophenyl-alpha-D-galactopyranoside
Bispora sp.
-
pH 5.5, 37C
3.7
-
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant A41Y, pH 7.4, 30C
4.5
-
4-nitrophenyl-alpha-D-galactopyranoside
-
wild-type, pH 7.4, 30C
0.048
-
4-O-methylphenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.088
-
4-O-methylphenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.62
-
6-bromo-2-naphthyl alpha-D-galactoside
-
alpha-galactosidase II
1.8
-
6-bromo-2-naphthyl alpha-D-galactoside
-
alpha-galactosidase I
1
-
alpha-phenylgalactoside
Coffea sp.
-
alpha-galactosidase II
-
2
-
alpha-phenylgalactoside
Coffea sp.
-
alpha-galactosidase I
-
0.039
-
beta-naphthyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.225
-
beta-naphthyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
2
-
digalactosyl diacylglycerol
-
at 37C, pH 8.6
6.25
-
ethyl alpha-D-galactoside
-
-
8
-
ethyl alpha-D-galactoside
-
alpha-galactosidase II
8.93
-
ethyl alpha-D-galactoside
-
alpha-galactosidase I
0.28
-
Galabiosylceramide
-
-
0.13
-
galactinol
-
alpha-galactosidase I
0.69
-
galactinol
-
alpha-galactosidase II
44
-
galactomannan with galactose/mannose ratio 1:1.72
-
pH 5.0, 50C
-
34
-
galactomannan with galactose/mannose ratio 1:2.4
-
pH 5.0, 50C
-
3.7
-
globopentaose
-
-
9.1
-
globotriose
-
-
0.18
-
globtriglycosylceramide
-
-
-
0.83
-
m-chlorophenyl alpha-D-galactoside
-
alpha-galactosidase I
1.17
-
m-chlorophenyl alpha-D-galactoside
-
alpha-galactosidase II
8.33
-
m-chlorophenyl alpha-D-galactoside
-
-
1.38
-
m-cresyl alpha-D-galactoside
-
alpha-galactosidase I
2
-
m-cresyl alpha-D-galactoside
-
alpha-galactosidase II
8.33
-
m-cresyl alpha-D-galactoside
-
-
1.57
-
m-nitrophenyl alpha-D-galactoside
-
-
2
-
m-nitrophenyl alpha-D-galactoside
-
-
2.5
-
m-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
10
-
m-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
0.72
-
m-nitrophenyl-alpha-D-galactopyranoside
-
-
1.1
-
m-nitrophenyl-alpha-D-galactopyranoside
-
-
0.00135
-
melibiose
-
-
0.39
-
melibiose
-
pH 4.5, 50C, isoenzyme alpha-Gal II
0.77
-
melibiose
-
alpha-galactosidase II
0.8
-
melibiose
-
-
0.96
-
melibiose
-
alpha-galactosidase I
1
-
melibiose
-
pH 4.5, 50C, isoenzyme alpha-Gal I
1.3
-
melibiose
-
-
1.4
-
melibiose
Q9Z4N5, -
pH 6.0, 36C; pH 6, 36C
1.5
-
melibiose
-
-
1.7
-
melibiose
-
30C, pH 5.8
1.75
-
melibiose
Gibberella sp.
C6FJG8
37C
1.92
-
melibiose
-
pH 5, 40C
2
-
melibiose
Corynebacterium murisepticum
-
-
2.12
-
melibiose
-
pH 5.0, 55C, intracellular enzyme
2.14
-
melibiose
-
pH 5.5, 37C
2.24
-
melibiose
-
-
2.3
-
melibiose
-
alpha-galactosidase II
2.33
-
melibiose
-
-
2.4
-
melibiose
-
50C, pH 4.5
3
-
melibiose
-
larger form
3.5
-
melibiose
-
smaller form
3.7
-
melibiose
-
alpha-galactosidase I
3.8
-
melibiose
-
alpha-galactosidase II
4.3
-
melibiose
-, Q2XQ11
-
5.37
-
melibiose
Tachigali multijuga
-
pH 5.0, 40C
5.53
-
melibiose
-
enzyme form P2
5.8
-
melibiose
-
mutant A41Y, pH 7.4, 30C
6.3
-
melibiose
-
30C, pH 5.0
7.39
-
melibiose
-
pH 5, 40C
7.9
-
melibiose
-
-
11.2
-
melibiose
-
wild-type, pH 7.4, 30C
12
-
melibiose
-
pH 5, 37C
12.58
-
melibiose
-
pH 5.5, 45C
15.11
-
melibiose
-
pH 7, 98C
19
-
melibiose
-
alpha-galactosidase I
20.14
-
melibiose
-
pH 5, 40C
20.8
-
melibiose
-
alpha-galactosidase I
24
-
melibiose
-
-
25.4
-
melibiose
-
at 37C, pH 8.6
33
-
melibiose
Rhizopus sp.
-
in 0.1 M McIlvaine buffer (pH 4.8) at 37C
45
-
melibiose
-
alpha-galactosidase II
254
-
melibiose
-
mutant Q251A, pH 7.4, 30C
5.3
-
methyl alpha-D-galactoside
-
alpha-galactosidase I
7.13
-
methyl alpha-D-galactoside
-
alpha-galactosidase I
8.4
-
methyl alpha-D-galactoside
-
alpha-galactosidase II
10.9
-
methyl alpha-D-galactoside
-
-
14.3
-
methyl alpha-D-galactoside
-
alpha-galactosidase II
5.88
-
n-propyl alpha-D-galactoside
-
alpha-galactosidase II
6.13
-
n-propyl alpha-D-galactoside
-
alpha-galactosidase I
6.25
-
n-propyl alpha-D-galactoside
-
-
0.78
-
o-cresyl alpha-D-galactoside
-
alpha-galactosidase II
1.33
-
o-cresyl alpha-D-galactoside
-
alpha-galactosidase I
4.54
-
o-cresyl alpha-D-galactoside
-
-
8.8
-
o-nitrophenyl alpha-D-fucopyranoside
-
-
0.33
-
o-nitrophenyl alpha-D-galactoside
-
-
0.69
-
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
1.14
-
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
1.3
-
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase III
1.4
-
o-nitrophenyl alpha-D-galactoside
-
-
2.3
-
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
3.8
-
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
0.26
-
o-nitrophenyl-alpha-D-galactopyranose
-
-
0.84
-
o-nitrophenyl-alpha-D-galactopyranoside
-
-
2.27
-
o-nitrophenyl-alpha-D-galactopyranoside
-
-
1.3
-
o-nitrophenyl-alpha-N-acetyl-galactosaminide
-
alpha-galactosidase B
0.2
-
o-nitrophenyl-beta-D-galactopyranose
-
alpha-galactosidase II
0.3
-
o-nitrophenyl-beta-D-galactopyranose
-
alpha-galactosidase II
0.87
-
p-aminophenyl alpha-D-galactoside
-
alpha-galactosidase II
0.95
-
p-aminophenyl alpha-D-galactoside
-
alpha-galactosidase I
1
-
p-cresyl alpha-D-galactoside
-
alpha-galactosidase II
-
1.54
-
p-cresyl alpha-D-galactoside
-
alpha-galactosidase I
-
4.76
-
p-cresyl alpha-D-galactoside
-
-
-
4.76
-
p-nitrophenyl alpha-D-fucoside
-
alpha-galactosidase I
5.88
-
p-nitrophenyl alpha-D-fucoside
-
alpha-galactosidase II
0.075
-
p-nitrophenyl alpha-D-galactopyranoside
-
pH 5, 75C
0.085
-
p-nitrophenyl alpha-D-galactopyranoside
Q97U94, -
in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80C
0.22
-
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50C, isoenzyme alpha-Gal II
0.221
-
p-nitrophenyl alpha-D-galactopyranoside
-
-
0.24
-
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50C, isoenzyme alpha-Gal IV
0.27
-
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50C, isoenzyme alpha-Gal III
0.29
0.35
p-nitrophenyl alpha-D-galactopyranoside
-
-
0.4
-
p-nitrophenyl alpha-D-galactopyranoside
-
pH 6.0, 40C
0.52
-
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-b2
0.54
-
p-nitrophenyl alpha-D-galactopyranoside
-
at 30C
0.69
-
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-m2
0.74
-
p-nitrophenyl alpha-D-galactopyranoside
-
pH 7, 98C
0.76
-
p-nitrophenyl alpha-D-galactopyranoside
-
enzyme form P2
1.1
-
p-nitrophenyl alpha-D-galactopyranoside
-
at 55C
1.13
-
p-nitrophenyl alpha-D-galactopyranoside
-
at 55C, in 50 mM citrate buffer (pH 4.2)
1.4
-
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50C, isoenzyme alpha-Gal I
1.55
-
p-nitrophenyl alpha-D-galactopyranoside
-
enzyme form P1
1.89
-
p-nitrophenyl alpha-D-galactopyranoside
-
-
2.9
-
p-nitrophenyl alpha-D-galactopyranoside
Rhizopus sp.
-
in 0.1 M McIlvaine buffer (pH 4.8) at 37C
3.2
-
p-nitrophenyl alpha-D-galactopyranoside
-
30C, pH 5.0
4.7
-
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-b1
4.7
-
p-nitrophenyl alpha-D-galactopyranoside
Q9WXC1
pH 6.0, 70C
4.72
-
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-m1
7.59
-
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-m3
7.9
-
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-b3
12
-
p-nitrophenyl alpha-D-galactopyranoside
-
30C, pH 5.8
0.28
-
p-nitrophenyl alpha-D-galactose
-
alpha-galactosidase I
0.56
-
p-nitrophenyl alpha-D-galactose
-
alpha-galactosidase II
0.08
-
p-nitrophenyl alpha-D-galactoside
-
-
0.12
-
p-nitrophenyl alpha-D-galactoside
-
-
0.15
-
p-nitrophenyl alpha-D-galactoside
-
tetramer
0.22
-
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
0.22
-
p-nitrophenyl alpha-D-galactoside
-
larger form
0.26
-
p-nitrophenyl alpha-D-galactoside
-
-
0.31
-
p-nitrophenyl alpha-D-galactoside
-
-
0.32
-
p-nitrophenyl alpha-D-galactoside
-
smaller form
0.35
-
p-nitrophenyl alpha-D-galactoside
-
alpha-D-galactosidase A
0.38
-
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
0.38
-
p-nitrophenyl alpha-D-galactoside
-
-
0.39
-
p-nitrophenyl alpha-D-galactoside
-
monomer
0.42
-
p-nitrophenyl alpha-D-galactoside
-
-
0.45
-
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
0.47
-
p-nitrophenyl alpha-D-galactoside
-
-
0.53
-
p-nitrophenyl alpha-D-galactoside
-
-
0.54
-
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
0.71
-
p-nitrophenyl alpha-D-galactoside
-
-
0.76
-
p-nitrophenyl alpha-D-galactoside
-
-
0.8
-
p-nitrophenyl alpha-D-galactoside
-
-
1
-
p-nitrophenyl alpha-D-galactoside
-
-
1
-
p-nitrophenyl alpha-D-galactoside
-
-
1.2
-
p-nitrophenyl alpha-D-galactoside
-
-
1.5
-
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
2.2
-
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II2
5.3
-
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II1
6
-
p-nitrophenyl alpha-D-galactoside
-
-
12.5
-
p-nitrophenyl beta-L-arabinoside
-
alpha-galactosidase II
14.3
-
p-nitrophenyl beta-L-arabinoside
-
alpha-galactosidase I
33.3
-
p-nitrophenyl beta-L-arabinoside
-
-
0.16
-
p-nitrophenyl-alpha-D-galactopyranose
-
-
0.17
-
p-nitrophenyl-alpha-D-galactopyranose
Corynebacterium murisepticum
-
-
0.23
-
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase II, pH 6
0.32
-
p-nitrophenyl-alpha-D-galactopyranoside
-
-
0.47
-
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase I, pH 7
0.53
-
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase II, pH 7
0.59
-
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase I, pH 6
0.75
-
p-nitrophenyl-alpha-D-galactopyranoside
-
-
0.83
-
p-nitrophenyl-alpha-D-galactopyranoside
-
-
1.46
-
p-nitrophenyl-alpha-D-galactopyranoside
-
-
0.26
-
p-nitrophenyl-alpha-D-galactoside
-
cell wall alpha-galactosidase I
0.29
-
p-nitrophenyl-alpha-D-galactoside
-
protein bodies alpha-galactosidase I
0.31
-
p-nitrophenyl-alpha-D-galactoside
-
cell wall alpha-galactosidase II
0.65
-
p-nitrophenyl-alpha-D-galactoside
Q9Z4N5, -
pH 6, 36C
1.09
-
p-nitrophenyl-alpha-D-galactoside
-
alpha-galactosidase 3
1.35
-
p-nitrophenyl-alpha-D-galactoside
-
alpha-galactosidase 2
1.36
-
p-nitrophenyl-alpha-D-galactoside
-
alpha-galactosidase 1
0.2
-
p-nitrophenyl-beta-D-galactopyranose
-
alpha-galactosidase II
0.4
-
p-nitrophenyl-beta-D-galactopyranose
-
alpha-galactosidase II
37.4
-
p-nitrophenyl-beta-L-arabinopyranoside
Q97U94, -
in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80C
0.176
-
phenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.324
-
phenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
1.11
-
phenyl alpha-D-galactoside
-
alpha-galactosidase I
1.25
-
phenyl alpha-D-galactoside
-
alpha-galactosidase II
5
-
phenyl alpha-D-galactoside
-
-
6.9
-
planteose
-
-
0.42
-
raffinose
-
pH 5.0, 65C
0.49
-
raffinose
-
pH 5.0, 40C
0.5
-
raffinose
-
-
0.66
-
raffinose
-
pH 5.5, 37C
1.6
-
raffinose
-
alpha-galactosidase II2
1.61
-
raffinose
-
at 55C, in 50 mM citrate buffer (pH 4.2)
1.8
-
raffinose
-
alpha-galactosidase I
2
3
raffinose
-
pH 5.0, 40C
2.1
-
raffinose
-
-
2.1
-
raffinose
-
pH 5, 75C
2.16
-
raffinose
-
-
2.7
-
raffinose
-
alpha-galactosidase II
3.65
-
raffinose
-
-
3.8
-
raffinose
-
-
4
-
raffinose
-
alpha-galactosidase I
4.4
-
raffinose
-
30C, pH 5.0
4.6
-
raffinose
-
alpha-galactosidase I
4.8
-
raffinose
-
30C, pH 5.8
5
-
raffinose
-
alpha-galactosidase II
5
-
raffinose
-
alpha-galactosidase II1
5
-
raffinose
-
smaller form
5.32
-
raffinose
-
pH 7, 98C
5.34
-
raffinose
-
enzyme form P2
5.9
-
raffinose
-
alpha-galactosidase II
6.5
-
raffinose
-
larger form
11.3
-
raffinose
-
50C, pH 4.5
11.8
-
raffinose
-
-
12.5
-
raffinose
-
-
12.6
-
raffinose
-
-
15
-
raffinose
-
pH 5, 37C
18
-
raffinose
-
at 37C, pH 8.6
24.1
-
raffinose
-
-
25.9
-
raffinose
-
-
26.3
-
raffinose
-
alpha-galactosidase III
27.7
-
raffinose
-
alpha-galactosidase I
27.93
-
raffinose
-
pH 5, 40C
32.8
-
raffinose
-
pH 5.0, 55C, intracellular enzyme
32.99
-
raffinose
-
pH 5, 40C
36.4
-
raffinose
-
-
39.62
-
raffinose
Tachigali multijuga
-
pH 5.0, 40C
40
-
raffinose
-
-
54.1
-
raffinose
-
wild-type, pH 7.4, 30C
54.26
-
raffinose
Gibberella sp.
C6FJG8
37C
71.4
-
raffinose
-
alpha-galactosidase II
80
-
raffinose
-
-
87.9
-
raffinose
-
mutant A41Y, pH 7.4, 30C
98.1
-
raffinose
-
alpha-galactosidase I
105
-
raffinose
-
pH 7.5, temperature not specified in the publication
0.22
-
stachyose
-
pH 5.0, 40C
0.3
-
stachyose
-
alpha-galactosidase II
0.33
-
stachyose
-
pH 5.0, 65C
1.15
-
stachyose
-
-
1.17
-
stachyose
-
at 55C, in 50 mM citrate buffer (pH 4.2)
1.8
-
stachyose
-
-
2.4
-
stachyose
-
alpha-galactosidase I
3.8
-
stachyose
-
alpha-galactosidase II
3.8
-
stachyose
-
30C, pH 5.0
4.5
-
stachyose
-
-
5.26
-
stachyose
-
alpha-galactosidase II
7.2
-
stachyose
-
30C, pH 5.8
7.5
-
stachyose
-
alpha-galactosidase I
7.8
-
stachyose
-
at 37C, pH 8.6
8.23
-
stachyose
Gibberella sp.
C6FJG8
37C
8.5
-
stachyose
-
alpha-galactosidase I
10
-
stachyose
-
larger form
10.8
-
stachyose
-
pH 5.0, 55C, intracellular enzyme
10.94
-
stachyose
-
pH 5, 40C
11
-
stachyose
-
alpha-galactosidase I
14.76
-
stachyose
-
pH 5.5, 45C
15
-
stachyose
-
alpha-galactosidase II1
21
-
stachyose
-
pH 5.0, 40C
30
-
stachyose
-
smaller form
33.3
-
stachyose
-
alpha-galactosidase III
35.5
-
stachyose
-
alpha-galactosidase I
48.8
-
stachyose
Tachigali multijuga
-
pH 5.0, 40C
54.74
-
stachyose
-
pH 5, 40C
72
-
stachyose
-
alpha-galactosidase II
4.2
-
verbascose
-
at 37C, pH 8.6
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.085
-
2,4-dinitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.41
-
2,4-dinitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
12.23
-
2,4-dinitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.042
-
2,5-dinitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
11.2
-
2,5-dinitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.035
-
2-nitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
-
0.52
-
2-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
-
13.67
-
2-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
-
0.01
-
3,4-dimethylphenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.052
2.1
3,4-dimethylphenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
10.39
-
3,4-dimethylphenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.042
-
3-nitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
10.5
-
3-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.016
-
4-bromophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
12.58
-
4-bromophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
49.8
-
4-methylumbelliferyl alpha-D-galactoside
-
50C, pH 4.5
-
7
-
4-nitrophenyl alpha-D-galactopyranoside
-
pH 5.0, 40C
1800
-
4-nitrophenyl alpha-D-galactopyranoside
-
pH 5.0, 40C
0.00218
-
4-nitrophenyl alpha-D-galactoside
-, Q8A6L0
mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
0.03
-
4-nitrophenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.0684
-
4-nitrophenyl alpha-D-galactoside
-, Q8A6L0
mutant enzyme E470Q, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
8.58
-
4-nitrophenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
244
-
4-nitrophenyl alpha-D-galactoside
-, Q8A6L0
wild type enzyme, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
3279
-
4-nitrophenyl alpha-D-galactoside
-
-
0.12
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 4.5, 37C
0.23
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 9.0, 37C
0.3
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 6.7, 37C
0.62
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 8.7, 37C
1.05
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 7.1, 37C
1.37
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 8.4, 37C
1.68
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 7.5, 37C
1.75
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 8.0, 37C
2.07
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 7.8, 37C
3.29
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5.0, 55C, intracellular enzyme
16
-
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant Q251W, pH 7.4, 30C
90
-
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant A41Y, pH 7.4, 30C
170
-
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant Q251A, pH 7.4, 30C
286
-
4-nitrophenyl-alpha-D-galactopyranoside
-
wild-type, pH 7.4, 30C
654.6
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5.5, 45C
1278
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 37C
0.01
-
4-O-methylphenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.03
0.55
4-O-methylphenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
6.69
-
4-O-methylphenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.021
-
beta-naphthyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
6.26
-
beta-naphthyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
7
-
galactomannan with galactose/mannose ratio 1:1.72
-
pH 5.0, 50C
-
10
-
galactomannan with galactose/mannose ratio 1:2.4
-
pH 5.0, 50C
-
0.822
-
m-nitrophenyl-alpha-D-galactopyranoside
-
-
0.03
-
melibiose
-
pH 5.0, 55C, intracellular enzyme
2.3
-
melibiose
-
50C, pH 4.5
2.33
-
melibiose
-
pH 5.5, 45C
113
-
melibiose
-
mutant Q251A, pH 7.4, 30C
131
-
melibiose
-
mutant A41Y, pH 7.4, 30C
193
-
melibiose
-
wild-type, pH 7.4, 30C
234.2
-
melibiose
-
pH 7, 98C
1067
-
melibiose
-
pH 5, 37C
0.238
-
o-nitrophenyl-alpha-D-galactopyranoside
-
-
176
-
p-nitrophenyl alpha-D-galactopyranoside
-
pH 5, 75C
302.9
-
p-nitrophenyl alpha-D-galactopyranoside
-
pH 7, 98C
3914
-
p-nitrophenyl alpha-D-galactopyranoside
-
at 55C, in 50 mM citrate buffer (pH 4.2)
0.027
-
phenyl alpha-D-galactoside
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
5.71
-
phenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.89
-
raffinose
-
pH 5.0, 40C
4.12
-
raffinose
-
pH 5.0, 55C, intracellular enzyme
32.4
-
raffinose
-
50C, pH 4.5
46.8
-
raffinose
-
wild-type, pH 7.4, 30C
91
-
raffinose
-
mutant A41Y, pH 7.4, 30C
109
-
raffinose
-
pH 5, 75C
157.3
-
raffinose
-
pH 7, 98C
586
-
raffinose
-
pH 5, 37C
1100
-
raffinose
-
pH 5.0, 40C
6946
-
raffinose
-
at 55C, in 50 mM citrate buffer (pH 4.2)
0.57
-
stachyose
-
pH 5.0, 40C
6.05
-
stachyose
-
pH 5.0, 55C, intracellular enzyme
42.04
-
stachyose
-
pH 5.5, 45C
1250
-
stachyose
-
pH 5.0, 40C
7659
-
stachyose
-
at 55C, in 50 mM citrate buffer (pH 4.2)
3.61
-
p-nitrophenyl-alpha-D-galactopyranoside
-
-
additional information
-
phenyl alpha-D-galactoside
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0115
-
4-nitrophenyl alpha-D-galactoside
-, Q8A6L0
mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
252901
0.213
-
4-nitrophenyl alpha-D-galactoside
-, Q8A6L0
mutant enzyme E470Q, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
252901
778
-
4-nitrophenyl alpha-D-galactoside
-, Q8A6L0
wild type enzyme, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
252901
0.091
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 4.5, 37C
218817
0.81
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 6.7, 37C
218817
0.96
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 9.0, 37C
218817
2.7
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 7.1, 37C
218817
5
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 8.7, 37C
218817
5.8
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 7.5, 37C
218817
8.9
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 8.0, 37C
218817
9.9
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 7.8, 37C
218817
15.6
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 8.4, 37C
218817
24
-
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant A41Y, pH 7.4, 30C
218817
63.5
-
4-nitrophenyl-alpha-D-galactopyranoside
-
wild-type, pH 7.4, 30C
218817
66
-
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant Q251W, pH 7.4, 30C
218817
121
-
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant Q251A, pH 7.4, 30C
218817
4730
-
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 37C
218817
0.45
-
melibiose
-
mutant Q251A, pH 7.4, 30C
12909
17
-
melibiose
-
wild-type, pH 7.4, 30C
12909
22
-
melibiose
-
mutant A41Y, pH 7.4, 30C
12909
88.9
-
melibiose
-
pH 5, 37C
12909
0.8
-
raffinose
-
mutant A41Y, pH 7.4, 30C
16125
0.9
-
raffinose
-
wild-type, pH 7.4, 30C
16125
39.1
-
raffinose
-
pH 5, 37C
16125
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.038
-
1-deoxygalactonojirimycin
-
pH 4.6, 37C, wild-type
0.047
-
1-deoxygalactonojirimycin
-
pH 4.6, 37C, mutant M51I
0.25
-
alpha-D-galactopyranosyl azide
-
at 30C
0.38
-
D-galactose
-
at 30C
0.7
-
D-galactose
-
pH 5.0, 55C, intracellular enzyme
2.74
-
D-galactose
Tachigali multijuga
-
pH 5.0, 40C
3.5
-
D-galactose
-
30C, pH 5.0
5.6
-
D-galactose
-
30C, pH 5.8
189
-
D-galactose
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
0.65
-
galactose
-
enzyme form P2
2.77
-
galactose
-
pH 4.5, 50C
0.095
-
galactostatin bisulfite
-
pH 4.6, 37C, wild-type
0.122
-
galactostatin bisulfite
-
pH 4.6, 37C, mutant M51I
9.2
-
gamma-1,4-galactonolactone
-
30C, pH 5.0
-
43.1
-
gamma-1,4-galactonolactone
-
30C, pH 5.8
-
7.7
-
melibiose
-
30C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
10.3
-
melibiose
-
30C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
9
-
raffinose
-
30C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
13.1
-
raffinose
-
30C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
25.4
-
raffinose
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
6.6
-
stachyose
-
30C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
11.6
-
stachyose
-
30C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
9.6
-
Tris
-
30C, pH 5.0
12
-
Tris
-
30C, pH 5.8
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
6.9
-
(2S,3R,4R,5S)-2-(hydroxymethyl)-5-[(methylamino)methyl]pyrrolidine-3,4-diol
-
-
8.1
-
(2S,3R,4R,5S)-2-[(ethylamino)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol
-
-
4.8e-05
-
1-deoxygalactonojirimycin
-
IC50 48 nM with mutant enzyme S65A
5.8e-05
-
1-deoxygalactonojirimycin
-
IC50 58 nM with mutant enzyme S65D
5.9e-05
-
1-deoxygalactonojirimycin
-
IC50 59 nM with wild-type enzyme
6.3e-05
-
1-deoxygalactonojirimycin
-
IC50: 63 nM with mutant enzyme S65T
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.000833
-
-
value about, grown under low temperature for at least 3 days
0.00167
-
-
value about, grown under normal temperature for at least 3 days
0.00167
-
-
value about, leaf, 12 to 20 days after anthesis
0.002083
-
-
value about, leaf, 8 days after anthesis
0.00375
-
-
value about, cold-sensitve cultivar, Guonong No. 41
0.004167
-
-
value about, cold-tolerant cultivar, Guonong No. 25
0.004167
-
-
value about, leaf, 2 days before anthesis, day of anthesis and 4 days after anthesis; value about, mesocarp tissue, 20 days after anthesis
0.0067
-
-
value about, mesocarp tissue, 16 days after anthesis
0.0083
-
-
value about, mesocarp tissue, 12 days after anthesis
0.0125
-
-
value about, mesocarp tissue, day of anthesis and 8 days after anthesis
0.015
-
-
value about, mesocarp tissue, 2 days before anthesis
0.02
-
-
D-glucose
0.02083
-
-
value about, mesocarp tissue, 4 days after anthesis
0.052
-
-
-
0.08
-
-
D-mannose
0.11
-
-, Q19AX0
crude extract, using 4-nitrophenyl alpha-D-galactopyranoside as substrate, in 0.1 M Na+-phosphate buffer (pH 7.3), 0.15 M NaCl at 20C
0.13
-
-
D-galactose
0.18
-
Q97U94, -
crude cell extract, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80C
0.21
-
Q6IYF5
cell-free extract
0.26
-
-
melibiose
0.3
-
-
raffinose
0.31
-
Q7XIV4, Q9FXT4
alpha-Gal III, crude extract
0.41
-
-
guar gum
0.42
-
-
stachyose
1.03
-
-
culture filtrate
1.2
-
Rhizopus sp.
-
crude filtrate
2.02
-
-
crude extract, in 0.1 M sodium acetate buffer, pH 5.0, at 40C
2.1
-
-
alpha-galactosidase 3
2.25
-
Q7XIV4, Q9FXT4
alpha-Gal II, crude extract
2.3
-
-
alpha-galactosidase I
3.09
-
-
-
3.33
-
-
crude extract, in 0.1 M sodium acetate buffer, pH 4.0, at 40C
3.5
-
-
pH 5.0, 50C, substrate galactomannan with galactose/mannose ratio 1:1.72
4.25
-
Q97U94, -
after 23fold purification, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80C
4.37
-
Q6IYF5
after 20.8fold purification
5.75
-
-
unpurified enzyme from culture broth
6.2
-
-
pH 5.0, 50C, substrate galactomannan with galactose/mannose ratio 1:2.4
6.56
-
-
-
9.13
-
Q7XIV4, Q9FXT4
alpha-Gal III, after 29.5fold purification
10.8
-
-
isoenzyme alpha-Gal III
11.52
-
-
isoenzyme alpha-Gal IV
12.3
-
-
-
14.2
-
-
enzyme form P1
14.46
-
-
alpha-galactosidase 1
14.8
-
-
-
15.4
-
-, Q9KAQ9
pH 7.4, 37C, presence of NAD+, Mn2+, dithiothreitol
15.9
-
-
unpurified enzyme from fermentation broth, using p-nitrophenyl alpha-D-galactopyranoside as a substrate
17.6
-
-
-
20
-
-
-
21.2
-
-
recombinant enzyme
21.95
-
-
isoenzyme alpha-Gal II
23.6
-
Penicillium duponti
-
-
24.5
-
-
-
28.3
-
-
alpha-galactosidase II
29.1
-
-
-
30
-
-
crude extract, in 0.1 M sodium acetate buffer, pH 5.0, at 40C
30.8
-
-
-
32
-
Coffea sp.
-
-
36.8
-
-
pH 5.0, 65C
41
-
-
alpha-galactosidase II
44
-
-
-
48.33
-
Gibberella sp.
C6FJG8
37C
49.9
-
Citrullus battich
-
-
52.1
-
-
alpha-galactosidase II, p-nitrophenyl-alpha-D-galactopyranose as subtrate
52.8
-
Q7XIV4, Q9FXT4
alpha-Gal II, after 23.5fold purification
53.26
-
-
alpha-galactosidase 2
55
-
-
after 27.2fold purification, in 0.1 M sodium acetate buffer, pH 5.0, at 40C
56.61
-
C7SEV1
after 2461.3fold purification, using 4-nitrophenyl alpha-D-galactoside as substrate, at 37C
62.5
-
-
enzyme form P2
67.3
-
-
after 20.20fold purification, in 0.1 M sodium acetate buffer, pH 4.0, at 40C
68.3
-
Candida javanica
-
-
68.7
-
-
alpha-galactosidase I
74.1
-
Coffea sp.
-
-
74.6
-
Rhizopus sp.
-
after 60.4fold purification
74.62
-
-
purified recombinant enzyme
77.13
-
-
alpha-galactosidase II
81.54
-
-
alpha-galactosidase I
82
-
-
alpha-galactosidase II
83.6
-
Tachigali multijuga
-
pH 5.0, 40C
94.5
-
Coffea sp.
-
-
98
-
-
pH 5.0, 40C, substrate 4-nitrophenyl-alpha-D-galactopyranoside
98.58
-
-
-
104.9
-
-
recombinant enzyme
119.7
-
Q9FXT4
-
129
-
-
alpha-galactosidase I
217
-
-
alpha-galactosidase II
224
-
-
activity with p-nitrophenyl alpha-D-galactopyranoside
225
-
-
activity with p-nitrophenyl alpha-D-galactopyranoside
230
-
-
larger form
231
-
-, Q19AX0
after 2100fold purification, using 4-nitrophenyl alpha-D-galactopyranoside as substrate, in 0.1 M Na+-phosphate buffer (pH 7.3), 0.15 M NaCl at 20C
247
-
Q9WXC1
-
256
-
-
pH 5.0, 50C, substrate raffinose; pH 5.0, 50C, substrate stachyose
285
-
-
alpha-galactosidase II
290
-
-
alpha-galactosidase I
295.6
-
-
-
400
-
-
after 388fold purification
452
-
-
alpha-galactosidase III
540
-
-
after 19.74fold purification, in 0.1 M sodium acetate buffer, pH 5.0, at 40C
619
-
-
after 108fold purification
1080
-
-
isoenzyme alpha-Gal I
1255
-
-
pH 5.0, 40C, substrate 4-nitrophenyl-alpha-D-galactopyranoside
1598
-
-
alpha-galactosidase I
1600
-
-
alpha-galactosidase I
1642
-
-
alpha-galactosidase II
1813
-
-
after 114fold purification, using p-nitrophenyl alpha-D-galactopyranoside as a substrate
2800
-
-
alpha-galactosidase IV
3530
-
-
-
4600
-
-
alpha-galactosidase II
4900
-
-
alpha-galactosidase III
27350
-
-
-
89150
-
-
-
229000
-
-
plasma alpha-galactosidase A
2060000
-
-
placental alpha-galactosidase A
4070000
-
-
spleen alpha-galactosidase A
additional information
-
-
assay described, enzyme activity measured in homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown, relative enzyme activities of whole fly homogenates low on substrates specific for alpha-galactosidase
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2.5
3.5
-
alpha-galactosidase II, p-nitrophenyl alpha-D-galactoside as substrate
2.5
4.5
-
-
3
3.5
-
alpha-galactosidase II
3
3.5
-
alpha-galactosidase I, p-nitrophenyl alpha-D-galactoside as substrate
3
4
-
alpha-galactosidase II
3
5
Calvatia cyanthiformis
-
o-nitrophenyl alpha-D-galactoside as substrate
3
5
-
raffinose as substrate
3
6.5
-
alpha-galactosidase I
3
-
-
alpha-galactosidase 2
3.2
3.8
-
-
3.5
4.5
-
1-naphthyl alpha-D-galactoside, 2-naphthyl alpha-D-galactoside or 6-bromo-2-naphthyl alpha-D-galactoside as substrate
3.5
4.5
-
alpha-galactosidase II, raffinose as substrate
3.5
5.3
-
melibiose as substrate
3.5
5.5
-
alpha-galactosidase I, raffinose as substrate
3.5
-
-
alpha-galactosidase 3
3.5
-
Bispora sp.
-
-
3.6
4.2
Coffea sp.
-
-
3.8
4.5
-
p-nitrophenyl alpha-D-galactoside as substrate
4
4.5
-
kidney
4
4.5
-
-
4
5
-
p-nitrophenyl alpha-D-galactoside as substrate
4
6
-
stachyose or o-nitrophenyl alpha-D-galactoside as substrate
4
6
-
9917S2, 9929S1, 053A1, 096S14
4
-
Candida javanica
-
-
4
-
-
p-nitrophenyl alpha-D-galactoside as substrate
4
-
Gibberella sp.
C6FJG8
-
4.2
4.8
-
melibiitol as substrate
4.2
-
-
-
4.2
-
-
wild-type and mutant enzymes S65T, S65A and S65D
4.3
-
-
4-methylumbelliferyl-alpha-D-galactopyranoside as substrate
4.5
5
-
alpha-galactosidase II
4.5
5.5
-
alpha-galactosidase II
4.5
6.5
-
098SA1
4.5
-
Citrullus battich
-
natural substrates
4.5
-
-
alpha-galactosidase I
4.5
-
-
raffinose as substrate
4.5
-
Cajanus indicus
-
alpha-galactosidase II
4.6
-
Aspergillus paxillus
-
phenyl alpha-D-galactoside as substrate
4.6
-
-
wild-type and mutant enzymes Q279E and R301Q
4.6
-
-
-
4.8
-
-
o-nitrophenyl-alpha-N-acetyl-galactosaminide as substrate
4.8
-
Rhizopus sp.
-
-
4.9
-
-
brain
5
5.5
-
alpha-galactosidase II
5
5.5
-
alpha-galactosidase II, p-nitrophenyl alpha-D-galactoside as substrate
5
5.5
Tachigali multijuga
-
-
5
6
-
depends on substrate
5
6.5
-
0117SA12
5
-
-
alpha-galactosidase I
5
-
Cajanus indicus
-
alpha-galactosidase I
5
-
-
enzyme form P1
5
-
Q9FXT4
-
5
-
Q7XIV4, Q9FXT4
;
5
-
-
intracellular enzyme
5
-
-
; assay at
5
-
Q7WWP9
assay at
5.2
-
-
uterus
5.2
-
-
-
5.2
-
-
larger form
5.3
-
Coffea sp.
-
alpha-galactosidase I, phenyl alpha-D-galactoside as substrate
5.3
-
-
alpha-galactosidase II
5.3
-
Coffea sp.
-
alpha-galactosidase I, phenyl alpha-D-galactoside as substrate
5.3
-
-
alpha-galactosidase II2
5.5
-
-
alpha-galactosidase II
5.5
-
-
smaller form
5.5
-
-
p-nitrophenyl alpha-D-galactoside as substrate
5.5
-
-
raffinose as substrate
5.5
-
-
enzyme form P2
5.5
-
-
alpha-galactosidase 1
5.5
-
-, Q2XQ11
-
5.5
-
-
highest activity
5.5
-
-
assay at
5.9
6.4
-
alpha-galactosidase I
5.9
-
-
alpha-galactosidase I
5.9
-
-
alpha-galactosidase II1
5.9
-
Citrullus battich
-
artificial substrates
6
6.5
-
alpha-galactosidase I, p-nitrophenyl alpha-D-galactoside as substrate
6
-
Coffea sp.
-
alpha-galactosidase II, phenyl alpha-D-galactoside as substrate
6
-
-
alpha-galactosidase II
6
-
-
alpha-galactosidase I
6
-
-
p-nitrophenyl alpha-D-galactoside as substrate
6.3
6.5
-
alpha-galactosidase II
6.3
-
Coffea sp., Vicia sativa
-
-
6.3
-
Coffea sp.
-
alpha-galactosidase II
6.3
-
Coffea sp.
-
-
6.5
-
-
alpha-galactosidase I
6.6
-
-
assay at, pH-optimum estimated for
6.7
7.7
-, Q19AX0
-
7
-
-
alpha-galactosidase II
7
-
-
at 90C in 50 mM sodium phosphate buffer
7.4
-
-
assay at
7.5
-
Corynebacterium murisepticum
-
-
7.5
-
Absidia sp.
-
-
7.5
-
-
assay at
7.5
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
6
-
pH 2.0: about 50% of maximal activity, pH 6.0: about 50% of maximal activity
2.5
-
-
complete loss of activity
3
5
Gibberella sp.
C6FJG8
more than 80% of maximum activity
3
6.5
-
pH 3.0: enzyme P1 shows about 60% of maximal activity, enzyme P2 shows about 70% of maximal activity, pH 6.5: enzyme p1 shows about 30% of maximal activity, enzyme P2 shows about 75% of maximal activity
3
7.2
Citrullus battich
-
-
3
7.5
-
wild type enzyme
3
8
-
pH 3.0: about 40% of maximal activity, pH 8.0: about 30% of maximal activity
3
9
Coffea sp.
-
-
3.4
9
-
at 4C
3.5
5.5
-
intracellular enzyme
3.5
6.5
-
mutant enzymes
3.5
7.5
-
-
3.6
5.4
-
pH 3.6: about 75% of maximal activity, pH 5.4: about 40% of maximal activity, wild-type enzyme and mutant enzymes S65T, S65A and E66D
4
6.5
-
pH 4.0: about 45% of maximal activity, pH 6.5: about 40% of maximal activity
4
7
-
at 5C for 72 h
4.3
6.5
Q6IYF5
more than 50% activity between pH 4.3 and 6.5
4.5
7
-
pH 4.0: about 15% of maximal activity, pH 4.5: about 90% of maximal activity, pH 7.0: about 45% of maximal activity
4.5
7
-
more than 50% of maximal activity at pH 4.5 and pH 7.0, at 75C
4.5
7.5
-
pH 4.5: about 40% of maximal activity, pH 7.5: about 40% of maximal activity
5
7
-
55% activity retained
5.5
10
-
pH 5.5: about 60% of maximal activity, pH 10.0: about 45% of maximal activity
7
-
-
complete loss of activity
7
-
Tachigali multijuga
-
complete loss of activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
22
-, Q19AX0
-
35
-
-
alpha-galactosidase 3
37
-
-
assay at
37
-
-
assay at
37
-
Q7WWP9
assay at
37
-
-
-
40
-
-
assay at
45
50
-
9917S2, 0117SA12, 098SA1
45
-
-
enzyme form P1
45
-
Q9FXT4
activity with p-nitrophenyl alpha-D-galactopyranoside
45
-
-
alpha-galactosidase 2
45
-
Q7XIV4, Q9FXT4
;
45
-
-
assay at
50
65
-
096S14, 053A1
50
-
-
enzyme form P2
50
-
Rhizopus sp.
-
-
50
-
-
-
50
-
-, Q2XQ11
-
50
-
Tachigali multijuga
-
-
50
-
-
optimal temperature
55
-
-
intracellular enzyme
55
-
-
optimal temperature
55
-
Bispora sp.
-
-
60
-
-
alpha-galactosidase II
60
-
Gibberella sp.
C6FJG8
-
60
-
-
optimal temperature
65
-
-
alpha-galactosidase 1
70
-
Candida javanica
-
-
73
-
Absidia sp.
-
-
75
-
Q9WXC1
reaction with p-nitrophenyl alpha-D-galactopyranoside
99
-
-
in 50 mM sodium phosphate buffer, pH 7
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
50
-
25C: about 35% of maximal activity, 50C: 40% of maximal activity
30
55
-
55% activity retained
30
70
-
30C: about 35% of maximal activity, 70C: about 90% of maximal activity
30
70
-
about 50% of maximal activity at 30C and at 70C, enzyme P1
35
52
Q6IYF5
50% activity is observed between 35C and 50C
35
55
-
35C: about 40% of maximal activity, 55C: about 40% of maximal activity, enzyme P2
37
-
Gibberella sp.
C6FJG8
60% of maximum activity
45
60
-
intracellular enzyme
50
75
-
50C: about 60% of maximal activity, 75C: about 50% of maximal activity
75
95
-
more than 50% of maximal activity at 75C and at 95C, at pH 5
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.5
-
-
AgaS-b3, chromatofocusing
3.9
-
-
isoelectric focusing
4.1
-
-
isoelectric focusing, intracellular enzyme
4.5
-
-
isoelectric focusing, isoenzyme alpha-Gal II
4.6
-
-
isoelectric focusing
4.8
-
-
isoelectric focusing
4.9
-
-
isoelectric focusing, pH-gradient 3-7
4.9
-
-
isoelectric focusing
4.9
-
-
isoelectric focusing, isoenzyme alpha-Gal III; isoelectric focusing, isoenzyme alpha-Gal IV
4.9
-
Q9SP05, -
isoelectric focusing
4.9
-
-
isoelectric focusing
4.9
-
-
calculation
4.9
-
-
isoelectric focusing
4.9
-
-
calculated
4.91
-
Q9SP05, -
isoelectric focusing
5
-
-
AgaS-m3, chromatofocusing
5
-
-
calculated
5.1
-
Gibberella sp.
C6FJG8
calculated
5.1
-
-
isoelectric focusing
5.1
-
-
isoelectric focusing, extracellular enzyme
5.4
-
C7SEV1
calculated from amino acid sequence
5.6
-
-, Q19AX0
calculated from amino acid sequence
6
-
-
AgaS-b2, chromatofocusing; AgaS-m2, chromatofocusing
7.1
-
-
AgaS-b1, chromatofocusing; AgaS-m1, chromatofocusing
7.1
-
-
isoelectric focusing
7.2
-
-
isoelectric focusing
7.2
-
-
isoelectric focusing, alpha-galactosidase 2
9.3
-
-
isoelectric focusing, alpha-galactosidase 3
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
abalone digestive gland; ADG cell line
Manually annotated by BRENDA team
-
alpha-galactosidase I
Manually annotated by BRENDA team
Bifidobacterium breve 203
-
-
-
Manually annotated by BRENDA team
-
of culture containing wheat bran or locus bean gum as carbon source
Manually annotated by BRENDA team
Thermomyces lanuginosus IMI 158749
-
-
-
Manually annotated by BRENDA team
-
alpha-galactosidase II
Manually annotated by BRENDA team
Q9SP05, -
micropylar and lateral
Manually annotated by BRENDA team
Solanum lycopersicum Mill.
-
micropylar and lateral
-
Manually annotated by BRENDA team
-
alpha-galactosidase 1; alpha-galactosidase 3; protein level of alpha-galactosidase 2 is low in developing fruits and generally increased with ripening
Manually annotated by BRENDA team