Information on EC 3.2.1.22 - alpha-galactosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY
3.2.1.22
-
RECOMMENDED NAME
GeneOntology No.
alpha-galactosidase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
show the reaction diagram
also hydrolyses alpha-D-fucosides
-
-
-
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
show the reaction diagram
the recombinant enzyme catalyzes the hydrolysis of phenyl R-galactosides via a redox elimination-addition mechanism involving oxidation of the hydroxyl group at C-3 and elimination of phenol across the C-1-C-2 bond to give an enzyme-bound glycal intermediate
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
melibiose degradation
-
-
stachyose degradation
-
-
metabolism of disaccharids
-
-
Galactose metabolism
-
-
Glycerolipid metabolism
-
-
Sphingolipid metabolism
-
-
Glycosphingolipid biosynthesis - globo series
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1,6-alpha-D-galactoside galactohydrolase
-
1,6-alpha-D-galactoside galactohydrolase
-
-
a-galactosidase
-
-
Aga-F78
Rhizopus sp.
-
-
Aga-F78
Rhizopus sp. F78
-
-
-
agaAJB13
gene name
agaAJB13
gene name
-
AgalB
Bispora sp.
-
-
Agalsidase alfa
-
-
-
-
Agalsidase alfa
-
-
AkalphaGal
-
-
alkaline alpha-gal form 1
-
-
alkaline alpha-galactosidase
-
-
alkaline alpha-galactosidase
-
-
alkaline alpha-galactosidase form 1
-
-
alpha-D-galactopyranoside galactohydrolase
-
-
alpha-D-galactopyranoside galactohydrolase
Talaromyces flavus CCF2686
-
-
-
alpha-D-galactosidase
-
-
-
-
alpha-D-galactosidase
-
-
alpha-D-galactosidase
-
-
alpha-D-galactosidase
-
alpha-D-galactosidase
-
-
alpha-D-galactosidase
Talaromyces flavus CCF2686
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
Aspergillus niger M47
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
-
Alpha-D-galactoside galactohydrolase
Geobacillus stearothermophilus NCIM 5146, Geobacillus stearothermophilus NCIM-5146
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
Penicillium chrysogenum LN33
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
Alpha-D-galactoside galactohydrolase
-
Alpha-D-galactoside galactohydrolase
Pseudoalteromonas sp. KMM701
-
-
Alpha-D-galactoside galactohydrolase
-
-
alpha-Gal
-
-
alpha-Gal
-
-
alpha-Gal
-
-
alpha-Gal
Pseudoalteromonas sp. KMM701
-
-
alpha-Gal A
-
alpha-Gal II
-
-
alpha-Gal III
-
alpha-gal1
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
Bispora sp.
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
-
alpha-galactosidase
-
alpha-galactosidase
-
-
alpha-galactosidase
Thalassococcus halodurans UST050418-052
-
-
-
alpha-galactosidase 1
-
-
alpha-galactosidase 2
-
-
alpha-galactosidase 3
-
-
alpha-galactosidase A
-
-
-
-
alpha-galactosidase A
-
-
alpha-galactosidase A
-
alpha-galactosidase AgaA A355E
-
-
alpha-galactosidase AgaB
-
-
alpha-galactosidase I
-
alpha-galactosidase II
-
-
alpha-galactosidase III
-
alpha-galactoside galactohydrolase
-
-
-
-
alpha-galactoside galactohydrolase
-
alphaGal1
-
-
alphaGal1
Talaromyces flavus CCF2686
-
-
-
E1 alpha-galactosidase
-
-
E2 alpha-galactosidase
-
-
E3 alpha-galactosidase
-
-
GH36 alpha-galactosidase
-
-
MelA
-
gene name
melibiase
-
-
-
-
retaining alpha-galactosidase
-
-
TmGalA
Thermotoga maritima DSMZ3109
-
-
-
TnGalA
Thermotoga neapolitana DSMZ5068
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9025-35-8
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Absidia sp.
strain WL511
-
-
Manually annotated by BRENDA team
Absidia sp. WL511
strain WL511
-
-
Manually annotated by BRENDA team
type Runner and type Spanish
-
-
Manually annotated by BRENDA team
NRRL 3135
-
-
Manually annotated by BRENDA team
Aspergillus ficuum NRRL 3135
NRRL 3135
-
-
Manually annotated by BRENDA team
strain M47
-
-
Manually annotated by BRENDA team
Aspergillus niger M47
strain M47
-
-
Manually annotated by BRENDA team
Aspergillus paxillus
-
-
-
Manually annotated by BRENDA team
growth on guar gum as sole carbon source
-
-
Manually annotated by BRENDA team
strain ATCC 29148
Uniprot
Manually annotated by BRENDA team
Bifidobacterium animalis subsp. lactis LAFTI B94
strain LAFTI B94
-
-
Manually annotated by BRENDA team
strain 203
-
-
Manually annotated by BRENDA team
strain 203
Uniprot
Manually annotated by BRENDA team
Bifidobacterium breve 203
strain 203
-
-
Manually annotated by BRENDA team
Bifidobacterium breve 203
strain 203
Uniprot
Manually annotated by BRENDA team
strain LAFTI Bl536
-
-
Manually annotated by BRENDA team
Bifidobacterium longum LAFTI Bl536
strain LAFTI Bl536
-
-
Manually annotated by BRENDA team
Bispora sp.
-
-
-
Manually annotated by BRENDA team
Cajanus indicus
-
-
-
Manually annotated by BRENDA team
Calvatia cyanthiformis
-
-
-
Manually annotated by BRENDA team
chestnut
-
-
Manually annotated by BRENDA team
watermelon
-
-
Manually annotated by BRENDA team
existence of at two isoforms, encoded by at least two genes
-
-
Manually annotated by BRENDA team
var. Robusta
Uniprot
Manually annotated by BRENDA team
Coffea sp.
green coffee beans
-
-
Manually annotated by BRENDA team
Corynebacterium murisepticum
-
-
-
Manually annotated by BRENDA team
a cold-tolerant cultivar, Guonong No. 25, and a cold sensitive cultivar, Guonong No. 41
-
-
Manually annotated by BRENDA team
strain UFV-1
-
-
Manually annotated by BRENDA team
Debaryomyces hansenii UFV-1
strain UFV-1
-
-
Manually annotated by BRENDA team
alpha-galactosidase AgaA; alpha-galactosidase AgaB
-
-
Manually annotated by BRENDA team
strain NCIM 5146
-
-
Manually annotated by BRENDA team
strain NCIM-5146
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus NCIM 5146
strain NCIM 5146
-
-
Manually annotated by BRENDA team
Geobacillus stearothermophilus NCIM-5146
strain NCIM-5146
-
-
Manually annotated by BRENDA team
Gibberella sp.
strain F75
UniProt
Manually annotated by BRENDA team
strain F75
UniProt
Manually annotated by BRENDA team
cultivar Monarca
-
-
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
L. cv. Giant Gray Stripe
-
-
Manually annotated by BRENDA team
male patients with Fabry disease
-
-
Manually annotated by BRENDA team
patients with Fabry disease
-
-
Manually annotated by BRENDA team
patients with sporadic Parkinson's disease
-
-
Manually annotated by BRENDA team
strains LAFTI L10 and La4962
-
-
Manually annotated by BRENDA team
strains LAFTI L26 and Lc279
-
-
Manually annotated by BRENDA team
strain R08, high alpha-galactosidase activity during the stationary phase in a medium where raffinose is the only carbon source
-
-
Manually annotated by BRENDA team
Lactobacillus curvatus R08
strain R08, high alpha-galactosidase activity during the stationary phase in a medium where raffinose is the only carbon source
-
-
Manually annotated by BRENDA team
subspecies Lactobacillus delbrueckii bulgaricus, strain Lb1466
-
-
Manually annotated by BRENDA team
strain CRL722
Uniprot
Manually annotated by BRENDA team
Lactobacillus fermentum CRL722
strain CRL722
Uniprot
Manually annotated by BRENDA team
strain JK55, high alpha-galactosidase activity during the stationary phase in a medium where raffinose is the only carbon source
-
-
Manually annotated by BRENDA team
Leuconostoc mesenteroides JK55
strain JK55, high alpha-galactosidase activity during the stationary phase in a medium where raffinose is the only carbon source
-
-
Manually annotated by BRENDA team
no activity in Thalassobius aestuarii
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius aestuarii JC2049
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius gelatinovorus
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius gelatinovorus IAM 12617
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius mediterraneus
-
-
-
Manually annotated by BRENDA team
no activity in Thalassobius mediterraneus XSM19
-
-
-
Manually annotated by BRENDA team
alpha-Gal II; variant Nipponbare
Uniprot
Manually annotated by BRENDA team
L. var. Nipponbare
Uniprot
Manually annotated by BRENDA team
variant Nipponbare
UniProt
Manually annotated by BRENDA team
Penicillium chrysogenum LN33
strain LN33
-
-
Manually annotated by BRENDA team
Penicillium duponti
-
-
-
Manually annotated by BRENDA team
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
-
-
Manually annotated by BRENDA team
VKM B-2135 D
UniProt
Manually annotated by BRENDA team
Pseudoalteromonas sp. KMM701
-
UniProt
Manually annotated by BRENDA team
Rhizopus sp.
18S rRNA sequence; strain F78 ACCC 30795
-
-
Manually annotated by BRENDA team
Rhizopus sp. F78
18S rRNA sequence; strain F78 ACCC 30795
-
-
Manually annotated by BRENDA team
; alpha-galactosidase precursor
SwissProt
Manually annotated by BRENDA team
sugar cane
-
-
Manually annotated by BRENDA team
Solanum lycopersicum Mill.
Mill.
SwissProt
Manually annotated by BRENDA team
Streptococcus thermophilus St1342
strain St1342
-
-
Manually annotated by BRENDA team
9917S2; 9929S1, 0177SA12, 096S14, 098SA1, 053A1
-
-
Manually annotated by BRENDA team
Streptomyces olivaceus 9917S2
9917S2
-
-
Manually annotated by BRENDA team
Tachigali multijuga
-
-
-
Manually annotated by BRENDA team
strain CCF2686
-
-
Manually annotated by BRENDA team
Talaromyces flavus CCF2686
strain CCF2686
-
-
Manually annotated by BRENDA team
UST050418-052
-
-
Manually annotated by BRENDA team
Thalassococcus halodurans UST050418-052
UST050418-052
-
-
Manually annotated by BRENDA team
strain CBS 395.62/b
-
-
Manually annotated by BRENDA team
Thermomyces lanuginosus CBS 395.62/b
strain CBS 395.62/b
-
-
Manually annotated by BRENDA team
Thermomyces lanuginosus IMI 158749
IMI 158749
-
-
Manually annotated by BRENDA team
strain MSB8
Uniprot
Manually annotated by BRENDA team
Thermotoga maritima DSMZ3109
DSMZ3109
-
-
Manually annotated by BRENDA team
Thermotoga neapolitana 5068
5068
-
-
Manually annotated by BRENDA team
Thermotoga neapolitana DSMZ5068
DSMZ5068
-
-
Manually annotated by BRENDA team
synonym Mortierella vinacea
UniProt
Manually annotated by BRENDA team
mung bean
-
-
Manually annotated by BRENDA team
biovar Microtus str. 91001
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
defects in human alpha-GAL lead to the development of Fabry disease, a lysosomal storage disorder characterized by the buildup of alpha-galactosylated substrates in the tissues
malfunction
-
dendritic cells deficient in alpha-galactosidase-A activity induce vigorous iNKT cell activation in the absence of exogenous antigen. Reconstitution of alpha-galactosidase-A-deficient dendritic cells with recombinant enzyme fully abrogate the iNKT cell response. Lack of alpha-galactosidase-A promote iNKT cell stimulation in vivo. Endogenous iNKT cells in Gla-/- hosts show signs of chronic exposure to self antigens
metabolism
-
stachyose biosynthesis
physiological function
-
AkalphaGal is a thylakoid membrane-degrading enzyme involved in the degradation of digalactosyl diacylglycerol during rice leaf senescence
physiological function
-
raffinose phloem unloading
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-azido-1-deoxy-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
?
1-naphthyl alpha-D-galactoside + H2O
1-naphthol + D-glucose
show the reaction diagram
-
-
-
?
2,4-dinitrophenyl alpha-D-galactoside + H2O
2,4-dinitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
2,5-dinitrophenyl alpha-D-galactoside + H2O
2,5-dinitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
2-naphthyl alpha-D-galactoside + H2O
2-naphthol + D-glucose
show the reaction diagram
-
-
-
?
2-nitrophenyl alpha-D-galactopyranoside + H2O
2-nitrophenol + D-galactopyranose
show the reaction diagram
-
16.5% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside
?
2-nitrophenyl alpha-D-galactoside + H2O
2-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
2-nitrophenyl alpha-D-galactoside + H2O
2-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
3,4-dimethylphenyl alpha-D-galactoside + H2O
3,4-dimethylphenol + alpha-D-galactose
show the reaction diagram
-
-
-
3-nitrophenyl alpha-D-galactoside + H2O
3-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
3-nitrophenyl-alpha-D-galactopyranoside + H2O
3-nitrophenol + D-galactopyranose
show the reaction diagram
-
5.6% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside
?
4-bromophenyl alpha-D-galactoside + H2O
4-bromophenol + alpha-D-galactose
show the reaction diagram
-
-
-
4-methoxyphenyl alpha-D-galactoside + H2O
4-methoxyphenol + alpha-D-galactose
show the reaction diagram
-
-
-
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
show the reaction diagram
-
-
-
-
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
4-methylumbelliferone + alpha-D-galactose
show the reaction diagram
Thermomyces lanuginosus IMI 158749
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
alpha-D-galactose + 4-methylumbelliferone
show the reaction diagram
-
-
-
?
4-methylumbelliferyl alpha-D-galactoside + H2O
alpha-D-galactose + 4-methylumbelliferone
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-alpha-D-galactoside + H2O
4-methylumbelliferol + alpha-D-galactose
show the reaction diagram
-
-
-
-
4-methylumbelliferyl-alpha-D-galactoside + H2O
4-methylumbelliferol + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
highest activity
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
best substrate
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
Pseudoalteromonas sp. KMM701
-
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
best substrate
-
?
4-nitrophenyl alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
Tachigali multijuga
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
Aspergillus niger M47, Penicillium chrysogenum LN33
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
-
-
?
4-nitrophenyl alpha-D-xylopyranoside + H2O
4-nitrophenol + alpha-D-xylopyranose
show the reaction diagram
-
9.64% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
show the reaction diagram
low activity
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
Bispora sp.
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + alpha-D-galactopyranose
show the reaction diagram
-
activity measured in total fly homogenates, substrate concentration of 60 mM in citrate buffer
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
Gibberella sp.
-
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
extracellular enzyme, intracellular enzyme
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
Debaryomyces hansenii UFV-1
-
extracellular enzyme, intracellular enzyme
-
?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
4-nitrophenol + D-galactopyranose
show the reaction diagram
-
-
?
4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
show the reaction diagram
-
-
-
?
4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
show the reaction diagram
-
-
?
4-O-[4-O[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]beta-D-mannopyranosyl]-beta-D-mannose + H2O
alpha-D-galactopyranose + 4-O-[4-O[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]beta-D-mannopyranosyl]-beta-D-mannose
show the reaction diagram
-
-
-
?
6(1)-alpha-D-galactosyl mannobiose + H2O
?
show the reaction diagram
2.6% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannobiose + H2O
?
show the reaction diagram
6.8% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannotriose + H2O
?
show the reaction diagram
2.7% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(1)-alpha-D-galactosyl mannotriose + H2O
?
show the reaction diagram
6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O
?
show the reaction diagram
0.5% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O
?
show the reaction diagram
7.3% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
6-bromo-2-naphthyl alpha-D-galactoside + H2O
6-bromo-2-naphthol + D-galactose
show the reaction diagram
-
-
-
?
6-bromo-2-naphthyl-alpha-D-galactopyranoside + H2O
6-bromo-2-naphthol + D-galactose
show the reaction diagram
Gibberella sp., Gibberella sp. F75
-
-
?
alpha-D-galactopyranosyl-(1-6)-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
alpha-D-galactose + beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose
show the reaction diagram
-
-
?
arabinogalactan + H2O
?
show the reaction diagram
-
alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3
-
-
?
beta-naphthyl alpha-D-galactoside + H2O
beta-naphthol + alpha-D-galactose
show the reaction diagram
-
-
-
carob galactomannan + H2O
?
show the reaction diagram
-
-
-
-
?
cellobiose + phenyl alpha-D-galactoside
alpha-D-Gal-D-Glc-beta-D-Glc-alpha-D-Gal-beta-D-Glc-D-Glc + alpha-D-Gal-beta-D-Glc-D-Glc
show the reaction diagram
Coffea sp.
-
-
-
?
cottonseed meal + H2O
?
show the reaction diagram
-
-
-
?
D-galactose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-galactose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
?
D-galactose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-glucose + melibiose
melibiose + D-glucose
show the reaction diagram
-
-
-
?
D-glucose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-glucose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-glucose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
D-glucose + raffinose
?
show the reaction diagram
-
-
-
-
?
D-mannose + phenyl alpha-D-galactoside
epimelibiose + H2O
show the reaction diagram
-
-
-
?
D-sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
16.30% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
D-sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
35.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
D-sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
Bifidobacterium animalis subsp. lactis LAFTI B94, Bifidobacterium longum LAFTI Bl536, Streptococcus thermophilus St1342
-
-
-
?
di-galactomannopentose + H2O
?
show the reaction diagram
-
-
-
-
?
digalactosyl diacylglycerol + H2O
?
show the reaction diagram
-
-
-
-
?
digalactosyldiacylglycerol + H2O
?
show the reaction diagram
-
galactolipid substrate, located in chloroplast thylakoid membranes, converted by the purified recombinant cutinase (EC 3.1.1.74) expressed in tobacco chloroplasts and showing alpha galactosidase activity, EC 3.2.1.22
-
-
?
ethanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
ethyl alpha-D-galactoside + H2O
ethanol + D-galactose
show the reaction diagram
-
-
-
?
galactan + H2O
?
show the reaction diagram
-
alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3
-
-
?
galactinol + H2O
alpha-D-galactopyranose + myoinositol
show the reaction diagram
-
-
-
?
galactinol + H2O
alpha-D-galactopyranose + myoinositol
show the reaction diagram
-
-
-
?
galactinol + H2O
alpha-D-galactopyranose + myoinositol
show the reaction diagram
-
-
-
?
galactobisylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
galactomannan + H2O
?
show the reaction diagram
-
-
-
-
?
galactomannan + H2O
?
show the reaction diagram
Tachigali multijuga
-
-
-
-
?
galactomannan + H2O
?
show the reaction diagram
-
with galactose/mannose ratios of 2.4 and of 1.72
-
-
?
galactomannotriose + H2O
?
show the reaction diagram
-
-
-
-
?
gentianose + phenyl alpha-D-galactoside
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
gentibiose-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
1.28fold increased activity compared to gentibiose
-
-
?
gentiobiose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
gentiobiose + phenyl alpha-D-galactoside
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
globopentaose + H2O
?
show the reaction diagram
-
-
-
-
?
globotriaosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
globotriaosylceramide + H2O
D-Galalpha(1,4)D-Glu-ceramide + D-galactose
show the reaction diagram
-
i.e. D-Galalpha(1,4)D-Galalpha(1,4)D-Glu-ceramide
-
?
globotriose + H2O
?
show the reaction diagram
-
-
-
-
?
globtriglycosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
glycerol + melibiose
floridoside + H2O
show the reaction diagram
Coffea sp.
-
-
-
?
glycerol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
Bispora sp.
-
-
-
-
?
guar gum + H2O
?
show the reaction diagram
0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
guar gum + H2O
?
show the reaction diagram
1.7% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
guar gum + H2O
?
show the reaction diagram
Talaromyces flavus CCF2686
-
-
-
-
?
isopentanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
isopropanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
lactose + H2O
beta-D-galactose + D-glucose
show the reaction diagram
-
-
-
?
lactose + H2O
beta-D-galactose + D-glucose
show the reaction diagram
-
enzyme P1: 6.2% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
lactose + H2O
beta-D-galactose + D-glucose
show the reaction diagram
-
2.3% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
lactose + phenyl alpha-D-galactoside
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
lactulose + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
Bispora sp.
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
locust bean gum + H2O
?
show the reaction diagram
1.4% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
-
?
locust bean gum + H2O
?
show the reaction diagram
Talaromyces flavus CCF2686
-
-
-
-
?
m-chlorophenyl alpha-D-galactoside + H2O
m-chlorophenol + D-galactose
show the reaction diagram
-
-
-
?
m-cresyl alpha-D-galactoside + H2O
m-cresol + D-galactose
show the reaction diagram
-
-
-
?
m-nitrophenyl alpha-D-galactoside + H2O
m-nitrophenol + D-galactose
show the reaction diagram
-
-
-
?
m-nitrophenyl alpha-D-galactoside + H2O
m-nitrophenol + D-galactose
show the reaction diagram
-
-
-
?
m-nitrophenyl alpha-D-galactoside + H2O
m-nitrophenol + D-galactose
show the reaction diagram
-
-
-
?
malitol + H2O
?
show the reaction diagram
-
-
-
-
?
malitol-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
2.66fold increased activity compared to malitol
-
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
-
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
enzyme P1: 4% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
10.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
18.12% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
maltose + H2O
alpha-D-glucose
show the reaction diagram
-
6.07% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
maltose + phenyl alpha-D-galactoside
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
maltose-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
3.19fold increased activity compared to maltose
-
-
?
mannitol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Coffea sp.
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Coffea sp.
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Coffea sp.
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Rhizopus sp.
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Tachigali multijuga
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Gibberella sp.
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
7.3% of Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
1.2% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
15.36% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
16.15% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
7.68% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
negligible hydrolytic activity towards melibiose
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
intracellular enzyme
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Rhizopus sp. F78
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Geobacillus stearothermophilus NCIM-5146
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Debaryomyces hansenii UFV-1
-
intracellular enzyme
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Talaromyces flavus CCF2686
-
negligible hydrolytic activity towards melibiose
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
42.1% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
35.5% of Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
enzyme P1: 6.9% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
Thermomyces lanuginosus IMI 158749
-
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
Bifidobacterium breve 203
-
-
-
?
melibiose + H2O
alpha-D-galactose + alpha-D-glucose
show the reaction diagram
-
-
?
melibiose + H2O
alpha-D-galactose + alpha-D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + alpha-D-glucose
show the reaction diagram
Thermotoga neapolitana, Thermotoga maritima, Thermotoga maritima DSMZ3109, Thermotoga neapolitana DSMZ5068
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
Bispora sp.
-
-
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
?
melibiose + melibiose
?
show the reaction diagram
-
-
-
-
?
melibiose + melibiose
mannotriose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + methanol
methyl alpha-D-galactoside + D-glucose
show the reaction diagram
Coffea sp.
-
-
-
?
melibiose-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
1.45fold decreased activity compared to melibiose
-
-
?
meso-inositol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
methyl alpha-D-galactoside + H2O
methanol + D-galactose
show the reaction diagram
-
-
-
?
methyl alpha-D-galactoside + H2O
methanol + D-galactose
show the reaction diagram
-
-
-
?
methyl alpha-D-galactoside + H2O
methanol + D-galactose
show the reaction diagram
-
-
-
?
N-acetyl-D-galactosamine + melibiose
6-O-alpha-D-galactosyl-N-acetyl-D-galactosamine + D-glucose
show the reaction diagram
-
-
-
?
N-acetyl-D-glucosamine + melibiose
6-O-alpha-D-galactosyl-N-acetyl-D-glucosamine + H2O
show the reaction diagram
-
-
-
?
n-butanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
n-propanol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
n-propyl alpha-D-galactoside + H2O
n-propanol + D-galactose
show the reaction diagram
-
-
-
?
O-beta-D-mannopyranosyl-(1-4)-O-[alpha-D-galactopyranosyl-(1-6)]-O-beta-D-mannopyranosyl-(1-4)-D-mannopyranosyl-(1-4)-D-mannopyranose + H2O
?
show the reaction diagram
-
-
-
?
o-cresyl alpha-D-galactoside + H2O
o-cresol + D-galactose
show the reaction diagram
-
-
-
?
o-nitrophenyl alpha-D-fucopyranoside + H2O
o-nitrophenol + D-fucose
show the reaction diagram
-
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
27.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + alpha-D-galactose
show the reaction diagram
Thermotoga maritima, Thermotoga maritima DSMZ3109
-
-
-
?
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
?
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
?
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
?
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
o-nitrophenyl alpha-D-galactoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
-
-
?
o-nitrophenyl beta-D-galactopyranoside
o-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
4.53% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
o-nitrophenyl-alpha-N-acetyl-galactosaminide + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranose + H2O
o-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
-
-
?
p-aminophenyl alpha-D-galactoside + H2O
p-aminophenol + D-galactose
show the reaction diagram
-
-
-
?
p-cresyl alpha-D-galactoside + H2O
p-cresol + D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-fucoside + H2O
p-nitrophenol + D-fucose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + D-glucose
?
show the reaction diagram
-
transglycosylation
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Rhizopus sp.
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Absidia sp.
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
100% activity
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Thermotoga maritima DSMZ3109
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Rhizopus sp. F78
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Thermomyces lanuginosus CBS 395.62/b
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Geobacillus stearothermophilus NCIM-5146
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Bifidobacterium animalis subsp. lactis LAFTI B94, Bifidobacterium longum LAFTI Bl536, Streptococcus thermophilus St1342
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Geobacillus stearothermophilus NCIM 5146
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Thermotoga neapolitana DSMZ5068
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Bifidobacterium breve 203
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Absidia sp. WL511
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
Talaromyces flavus CCF2686
-
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + D-galactose
show the reaction diagram
Solanum lycopersicum, Solanum lycopersicum Mill.
-
-
?
p-nitrophenyl alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
100% activity
-
?
p-nitrophenyl alpha-D-glucopyranoside + H2O
p-nitrophenol + alpha-D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl beta-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
enzyme P1: 5.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: no activity
-
?
p-nitrophenyl beta-L-arabinoside + H2O
p-nitrophenol + L-arabinose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
-
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
alpha-galactosidase 1, alpha-galactosidase 2, alpha-galactosidase 3
-
?
p-nitrophenyl-alpha-D-galactoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-galactopyranose + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-L-arabinopyranoside + H2O
p-nitrophenol + beta-L-arabinose
show the reaction diagram
-
-
?
phenyl alpha-D-galactoside + H2O
phenol + D-galactose
show the reaction diagram
-
-
-
?
phenyl alpha-D-galactoside + H2O
phenol + alpha-D-galactose
show the reaction diagram
-
-
-
phenyl alpha-D-galactoside + methanol
methyl alpha-D-galactoside + phenol
show the reaction diagram
-
-
-
?
planteose + H2O
?
show the reaction diagram
-
-
-
-
?
raffinose + galactinol
stachyose + H2O
show the reaction diagram
-
-
-
?
raffinose + H2O
?
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Prunus dulcis, Coffea sp.
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Coffea sp.
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Tachigali multijuga
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Gibberella sp.
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
worst substrate
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
30.60% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
60.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
78.57% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
intracellular enzyme
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Thermomyces lanuginosus CBS 395.62/b
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Geobacillus stearothermophilus NCIM-5146
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Bifidobacterium animalis subsp. lactis LAFTI B94, Bifidobacterium longum LAFTI Bl536, Streptococcus thermophilus St1342
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Debaryomyces hansenii UFV-1
-
intracellular enzyme
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
Thermotoga neapolitana DSMZ5068
-
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
worst substrate
-
?
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
-
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Bispora sp.
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
52.4% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
20% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
enzyme P1: 11.8% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 54.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
Vmax is 1.8fold higher than that with p-nitrophenyl alpha-D-galactopyranoside
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
1.89% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
10.1% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Thermomyces lanuginosus IMI 158749
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Thermotoga maritima DSMZ3109
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
Bifidobacterium breve 203
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
-
-
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
Rhizopus sp.
-
-
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
-
assay at pH 7.5, 37C, 15 min, reaction terminated by 2 min boiling
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
-
assay at pH 7.5, 37C, 20 min, reaction terminated by keeping the reaction mixture in boiling water for 2 min
-
?
raffinose + H2O
D-galactose + sucrose
show the reaction diagram
Rhizopus sp. F78
-
-
-
?
raffinose + melibiose
stachyose + H2O
show the reaction diagram
-
-
-
?
raffinose + methanol
methyl alpha-D-galactoside + verbascose + ajugose
show the reaction diagram
Coffea sp.
-
-
-
?
raffinose + raffinose
?
show the reaction diagram
-
-
-
-
?
raffinose-alternansucrase-derived oligosaccharide + ?
?
show the reaction diagram
-
1.67fold increased activity compared to raffinose
-
-
?
sorbitol + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
soybean meal + H2O
?
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Rhizopus sp.
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Bispora sp.
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
69.7% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
17.8% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
20fold less active than with raffinose
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
enzyme P1: 5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
high actvity with alpha-Gal I, low activity with alpha-Gal II
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
Vmax is 2fold higher than that with p-nitrophenyl alpha-D-galactopyranoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
0.47% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
1.9% relative activity compared to p-nitrophenyl alpha-D-galactoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
11.74% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
78.65% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
85.71% activity relative to p-nitrophenyl alpha-D-galactopyranoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Thermotoga maritima DSMZ3109
-
20fold less active than with raffinose
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Rhizopus sp. F78
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Thermomyces lanuginosus CBS 395.62/b
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Geobacillus stearothermophilus NCIM-5146
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Bifidobacterium animalis subsp. lactis LAFTI B94, Bifidobacterium longum LAFTI Bl536, Streptococcus thermophilus St1342
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Thermotoga neapolitana DSMZ5068
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
Bifidobacterium breve 203
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Coffea sp.
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
-
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Tachigali multijuga
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Gibberella sp.
-
-
-
?
stachyose + H2O
?
show the reaction diagram
Debaryomyces hansenii, Debaryomyces hansenii UFV-1
-
intracellular enzyme
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
stachyose + H2O
raffinose + D-galactose
show the reaction diagram
-
-
?
stachyose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
stachyose + methanol
methyl alpha-D-galactoside + verbascose + ajugose
show the reaction diagram
Coffea sp.
-
-
-
?
stachyose + stachyose
?
show the reaction diagram
-
-
-
-
?
sucrose + galactinol
raffinose + H2O
show the reaction diagram
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
?
sucrose + melibiose
raffinose + stachyose + planteose
show the reaction diagram
Coffea sp.
-
-
-
?
sucrose + melibiose
raffinose + D-glucose
show the reaction diagram
-
-
-
?
sucrose + phenyl alpha-D-galactoside
raffinose + stachyose + planteose
show the reaction diagram
Coffea sp.
-
-
-
?
sucrose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
sucrose + raffinose
planteose + D-glucose
show the reaction diagram
-
-
-
?
trehalose + phenyl alpha-D-galactoside
?
show the reaction diagram
-
-
-
-
?
trihexosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
show the reaction diagram
-
-
-
?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
show the reaction diagram
-
-
-
?
verbascose + H2O
alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
show the reaction diagram
-
20fold less active than with raffinose
-
?
methyl alpha-D-galactoside + H2O
methanol + D-galactose
show the reaction diagram
-
-
-
?
additional information
?
-
-
Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A
-
-
-
additional information
?
-
-
Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A. This enzyme is responsible for the hydrolysis of terminal alpha-galactosidase linkages in various glycolipids
-
-
-
additional information
?
-
-
raffinose induces about twice the activity than melibiose
-
-
-
additional information
?
-
transcription is induced by melibiose but is not totally repressed by glucose
-
-
-
additional information
?
-
-
activity with soy molasses
-
-
-
additional information
?
-
-
slight preference for branched galactosyl residues over straight chain residues
-
-
-
additional information
?
-
-
alpha-galactosidase 2 has the ability to markedly catalyze increased pectin solubility and depolymerization while the polymers are still structurally attached to the cell walls mimicking, in part, the changes that occur during ripening. The enzyme might contribute to papaya fruit softening during ripening
-
-
-
additional information
?
-
Fabry disease is an X-linked genetic disorder resulting from a deficiency of alpha-galactosidase activity
-
-
-
additional information
?
-
-
one of the principal enzymes involved in the modification or degradation of plant cell wall galactomannans
-
-
-
additional information
?
-
one of the principal enzymes involved in the modification or degradation of plant cell wall galactomannans
-
-
-
additional information
?
-
-
activity is not enhanced on sucrose and beta-linked carbohydrates and fructooligosaccharides
-
-
-
additional information
?
-
-
D-lactose, o-nitrophenyl beta-D-galactopyranoside, p-nitrophenyl alpha-D-glucopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates
-
-
-
additional information
?
-
-
D-sucrose, p-nitrophenyl alpha-D-glucopyranoside, p-nitrophenyl alpha-D-xylulopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates
-
-
-
additional information
?
-
-
lactose, o-nitrophenyl beta-D-galactopyranoside, p-nitrophenyl alpha-D-xylulopyranoside, p-nitrophenyl alpha-D-mannopyranoside, and p-nitrophenyl beta-D-galactopyranoside are no substrates
-
-
-
additional information
?
-
Rhizopus sp.
-
no detectable activity towards guar gum
-
-
-
additional information
?
-
-
no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal
-
-
-
additional information
?
-
no detectable enzymatic activity on xylo-, fuco-, manno-, or glucopyranosides
-
-
-
additional information
?
-
shows no activity toward p-nitrophenyl-alpha-substituted hexoses such as D-glucose, D-mannose, L-rhamnose, or N-acetyl-D-galactosaminide, p-nitrophenyl-beta-substituted hexoses such as D-galactose, D-glucose, and D-mannose do not support catalysis, nor does the pentose p-nitrophenyl-beta-D-xylopyranoside
-
-
-
additional information
?
-
enzyme additionally catalyzes transglycosylation. It is able to synthesize a trisaccharide galactose-(alpha-1,4)-galactose(alpha-1,6)-glucose using melibiose as substrate. In presence of 4-nitrophenyl-alpha-D-galactopyranoside, enzyme catalyzes glycosyl transfer to various acceptors
-
-
-
additional information
?
-
no substrate: 3-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galacturonide, 2-nitrophenyl-alpha-D-glucopyranoside, 2-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside, and 2-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside
-
-
-
additional information
?
-
no substrate: galactomannan
-
-
-
additional information
?
-
under optimum pH conditions and high melibiose concentration of 40% w/v, the enzyme is able to form oligosaccharides with degree of polymerisation of 3 and more at higher concentration than with degree of polymerization 2, with a total yield of 20.5% w/w
-
-
-
additional information
?
-
-
when treated with the Bacillus megaterium VHM1 enzyme, the flatulence-causing sugars in soymilk are completely hydrolyzed within 1.5 h
-
-
-
additional information
?
-
Rhizopus sp. F78
-
no detectable activity towards guar gum
-
-
-
additional information
?
-
Geobacillus stearothermophilus NCIM-5146
-
no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal
-
-
-
additional information
?
-
Thermotoga neapolitana DSMZ5068
-
slight preference for branched galactosyl residues over straight chain residues
-
-
-
additional information
?
-
Bifidobacterium breve 203
-
raffinose induces about twice the activity than melibiose
-
-
-
additional information
?
-
shows no activity toward p-nitrophenyl-alpha-substituted hexoses such as D-glucose, D-mannose, L-rhamnose, or N-acetyl-D-galactosaminide, p-nitrophenyl-beta-substituted hexoses such as D-galactose, D-glucose, and D-mannose do not support catalysis, nor does the pentose p-nitrophenyl-beta-D-xylopyranoside
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
globotriaosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
globotriaosylceramide + H2O
D-Galalpha(1,4)D-Glu-ceramide + D-galactose
show the reaction diagram
-
i.e. D-Galalpha(1,4)D-Galalpha(1,4)D-Glu-ceramide
-
?
guar gum + H2O
?
show the reaction diagram
-
-
-
-
?
locust bean gum + H2O
?
show the reaction diagram
-
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
-
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
Q19AX0
-
-
?
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
M4GRS1
-
-
?
melibiose + H2O
alpha-D-galactose + D-glucose
show the reaction diagram
-
42.1% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
melibiose + H2O
alpha-D-glucose + alpha-D-glucose
show the reaction diagram
-
-
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
o-nitrophenol + D-galactose
show the reaction diagram
-
27.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
p-nitrophenyl alpha-D-galactopyranoside + D-glucose
?
show the reaction diagram
-
transglycosylation
-
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
p-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
-
-
?
raffinose + H2O
sucrose + alpha-D-galactose
show the reaction diagram
-
52.4% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
-
-
?
stachyose + 2 H2O
2 alpha-D-galactose + sucrose
show the reaction diagram
-
69.7% of the activity with p-nitrophenyl alpha-D-galactopyranoside
-
?
sucrose + H2O
alpha-D-glucose + D-fructose
show the reaction diagram
-
-
-
?
trihexosylceramide + H2O
?
show the reaction diagram
-
-
-
-
-
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
M4GRS1
-
-
?
additional information
?
-
-
Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A
-
-
-
additional information
?
-
-
Fabry disease is a X-linked inborn error of glycolipid metabolism caused by deficiency of the lysosomal enzyme alpha-galactosidase A. This enzyme is responsible for the hydrolysis of terminal alpha-galactosidase linkages in various glycolipids
-
-
-
additional information
?
-
-
raffinose induces about twice the activity than melibiose
-
-
-
additional information
?
-
Q9L905
transcription is induced by melibiose but is not totally repressed by glucose
-
-
-
additional information
?
-
-
alpha-galactosidase 2 has the ability to markedly catalyze increased pectin solubility and depolymerization while the polymers are still structurally attached to the cell walls mimicking, in part, the changes that occur during ripening. The enzyme might contribute to papaya fruit softening during ripening
-
-
-
additional information
?
-
P06280
Fabry disease is an X-linked genetic disorder resulting from a deficiency of alpha-galactosidase activity
-
-
-
additional information
?
-
-
one of the principal enzymes involved in the modification or degradation of plant cell wall galactomannans
-
-
-
additional information
?
-
Q5DUH7
one of the principal enzymes involved in the modification or degradation of plant cell wall galactomannans
-
-
-
additional information
?
-
-
when treated with the Bacillus megaterium VHM1 enzyme, the flatulence-causing sugars in soymilk are completely hydrolyzed within 1.5 h
-
-
-
additional information
?
-
Bifidobacterium breve 203
-
raffinose induces about twice the activity than melibiose
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
required for enzymatic activity
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ag+
-
inhibits enzymatic activity
Ag2+
-
strong inhibitory effect
Ca2+
-
109.9% activity at 10 mM
Ca2+
-
slight inhibitory effect
Ca2+
119.7% activity at 10 mM
Co2+
strong activation
Cr3+
the activity of recombinant Aga-F78 is significantly enhanced in the presence of Cr3+
Cu2+
-
1 mM, 1.2fold stimulation
Cu2+
-
inhibits enzymatic activity
Cu2+
Bispora sp.
-
moderate stimulation
Cu2+
-
strong inhibitory effect
EDTA
-
activity enhanced by 2fold
Fe2+
strong activation
Fe3+
the activity of recombinant Aga-F78 is significantly enhanced in the presence of Fe3+
Hg2+
-
inhibits enzymatic activity
Hg2+
-
strong inhibitory effect
K+
-
activates the enzyme
K+
-
activates at 10 mM
K+
Rhizopus sp.
-
9.5% increased activity at 1 mM
K+
-
105.7% activity at 10 mM
Mg2+
-
activates at 10 mM
Mg2+
-
105.6% activity at 10 mM
Mn2+
-
activates at 10 mM
Mn2+
strong activation
Mn2+
-
activity enhanced by 2fold
Mn2+
-
required for enzymatic activity
Na+
-
124.8% activity at 10 mM
Na+
104.9% activity at 10 mM
Ni2+
moderate activation
Ni2+
Bispora sp.
-
moderate stimulation
Ni2+
-
slight inhibitory effect
Ni2+
113.7% activity at 10 mM
Pb2+
the activity of recombinant Aga-F78 is significantly enhanced in the presence of Pb2+
Pb2+
113.5% activity at 10 mM
SDS
-
inhibits enzymatic activity
Zn2+
moderate activation
Zn2+
-
activity enhanced by 2fold
Zn2+
Bispora sp.
-
moderate stimulation
Zn2+
-
slight inhibitory effect
Mn2+
110.8% activity at 10 mM
additional information
Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity; Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity
additional information
-
Ba2+, Ca2+, Co2+, Fe3+, K+, Mg2+, Mn2+, Na+, Ni2+, Zn2+ (1 mM) and EDTA (10 mM) have no influence on enzyme activity
additional information
the addition of other metal ions or chemicals has little or no effect on the activity
additional information
not influenced by Zn2+, Fe3+, K+, and Cu2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2S,3R,4R,5S)-2-(hydroxymethyl)-5-[(methylamino)methyl]pyrrolidine-3,4-diol
-
-
(2S,3R,4R,5S)-2-[(ethylamino)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol
-
-
1-deoxy-L-altronojirimycin hydrochloride
-
1000fold less effective than 1-deoxygalactonojirimycin
1-deoxygalactonojirimycin
-
IC50: 63 nM with mutant enzyme S65T, 48 nM with mutant enzyme S65A, 58 nM with mutant enzyme S65D and 59 nM with wild-type enzyme
1-deoxygalactonojirimycin
-
binding to the enzyme protein is enthalpy-driven and stronger than binding of galactostatin bisulfite. Inhibitor fits to the active-site pocket and undergoes hydrogen bonding with residues comprising the active-site pocket including the catalytic ones
1-deoxygalactonojirimycin
-
-
1-deoxygalactonojirimycin
-
-
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
31% residual activity at 100 mM, at pH 6.0
1-ethyl-3-(dimethylaminopropyl) carbodiimide
-
inactivation by 1-ethyl-3-(dimethylaminopropyl) carbodiimide plus glycine ethyl ester follows a two-phase kinetics: a fast one decreases in presence of melibiose and another slow not affected by melibiose
2,4,6-Trinitrobenzenesulfonic acid
-
20% residual activity at 2 mM, at pH 8.4
2-mercaptoethanol
-
98.66% activity at 10 mM
2-mercaptoethanol
-
95% activity at 10 mM
Ag+
1 mM, about 80% inhibition
Ag+
-
1 mM, 68% inhibition
Ag+
alpha-Gal II, 67% decrease of activity at 1 mM; alpha-Gal III, 84% decrease of activity at 1 mM
Ag+
Rhizopus sp.
-
no activity at 1 mM
Ag+
-
strong inhibitor
Ag+
-
complete inhibition at 10 mM
Ag+
-
potent inhibitor
Ag+
-
inactivation
Ag+
-
inactivation, intracellular enzyme
Ag+
Tachigali multijuga
-
-
Ag+
strong inhibitor
Ag+
-
inhibits enzymatic activity
Ag+
-
completely inhibits activity
Ag+
complete inhibition at 10 mM
Ag2+
1 mM, 46% inhibition
Ag2+
complete inhibition at 10 mM
Ag2+
Bispora sp.
-
strongly inhibits AgalB activity
AgNO3
-
1.0 mM, complete inhibition, alpha-galactosidase 1; 1.0 mM, complete inhibition, alpha-galactosidase 2; 1.0 mM, complete inhibition, alpha-galactosidase 3
Al3+
Rhizopus sp.
-
66.7% activity at 1 mM
alpha-D-galactopyranosyl azide
-
75% inhibition at 2 mM
alpha-D-galactose
-
2 mM, 17% inhibition of enzyme P1, 68.5% inhibition of enzyme P2
beta-D-galactose
competitive
Ca2+
-
strong inhibition at 10 mM
Ca2+
90.4% residual activity at 1 mM
Ca2+
Rhizopus sp.
-
82.2% activity at 1 mM
Ca2+
-
96.25% activity at 10 mM
Ca2+
-
94% activity at 10 mM
Ca2+
complete inhibition at 10 mM
CaCl2
-
10 mM, 19.9% inhibition
Cd2+
-
alpha-galactosidase II
Cd2+
86.0% residual activity at 1 mM
Cd2+
complete inhibition at 10 mM
CdSO4
-
0.25 mM, 99.9% inhibition
citraconic anhydride
-
30% residual activity at 5 mM, at pH 7.8
Co2+
-
alpha-galactosidase II
Co2+
-
slight inhibition at 10 mM
Co2+
Rhizopus sp.
-
96.6% activity at 1 mM
Co2+
complete inhibition at 10 mM
CoSO4
-
0.25 mM, 8.3% inhibition
Cr3+
Rhizopus sp.
-
63.9% activity at 1 mM
Cr3+
-
partial inhibition
Cu2+
-
1 mM, strong inhibition
Cu2+
-
slight inhibition at 10 mM
Cu2+
76.8% residual activity at 1 mM
Cu2+
Rhizopus sp.
-
78.1% activity at 1 mM
Cu2+
-
complete inhibition at 10 mM
Cu2+
-
59.16% activity at 10 mM
Cu2+
-
11% activity at 10 mM
Cu2+
complete inhibition at 10 mM
Cu2+
-
potent inhibitor
Cu2+
Gibberella sp.
strong
Cu2+
-
inactivation, intracellular enzyme
Cu2+
Tachigali multijuga
-
-
Cu2+
-
inhibits enzymatic activity
CuCl2
-
1.0 mM, 30.8% inhibition
CuCl2
-
1 mM, 50% inhibition
CuSO4
-
2 mM, 94.5% inhibition of enzyme P1, 48% inhibition of enzyme P2
D-fucose
-
-
D-fucose
-
75 mM, 11.1% inhibition, alpha-galactosidase 3; 75 mM, 25.5% inhibition, alpha-galactosidase 2; 75 mM, 47.1% inhibition, alpha-galactosidase 1
D-galactose
-
-
D-galactose
-
-
D-galactose
-
-
D-galactose
-
-
D-galactose
-
-
D-galactose
-
-
D-galactose
-
-
D-galactose
-
-
D-galactose
-
10 mM, complete inhibition
D-galactose
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
D-galactose
-
10 mM, 81.2% inhibition, alpha-galactosidase 1; 5 mM, 63% inhibition, alpha-galactosidase 2; 5 mM, 66.6% inhibition, alpha-galactosidase 3
D-galactose
-
-
D-galactose
-
inhibitory effect at 10 mM
D-galactose
-
51% inhibition at 2 mM
D-galactose
-
competitive, intracellular enzyme
D-galactose
Tachigali multijuga
-
competitive
D-galactose
-
inhibits enzymatic activity
D-glucose
-
-
D-glucose
-
-
D-glucose
-
94.4% activity at 10 mM
D-glucose
-
96% activity at 10 mM
D-lactose
-
89.51% activity at 10 mM
D-lactose
-
88% activity at 10 mM
D-maltose
-
92.8% activity at 10 mM
D-maltose
-
91.33% activity at 10 mM
D-maltose
-
94% activity at 10 mM
D-mannose
-
-
D-mannose
-
-
D-mannose
-
90.5% activity at 10 mM
D-mannose
-
90.05% activity at 10 mM
D-mannose
-
94% activity at 10 mM
D-sucrose
-
98.72% activity at 10 mM
D-xylose
-
10 mM, 12% inhibition
D-xylose
-
75 mM, 45% inhibition, alpha-galactosidase 3; 75 mM, 62.7% inhibition, alpha-galactosidase 2; 75 mM, 81.5% inhibition, alpha-galactosidase 1
D-xylose
-
49% inhibition at 2 mM
diethyldicarbonate
-
complete inhibition at 2 mM, at pH 6.0
EDTA
Rhizopus sp.
-
98.1% activity at 1 mM
EDTA
-
97.21% activity at 10 mM
Fe2+
Rhizopus sp.
-
75.1% activity at 1 mM
Fe2+
-
partial inhibition
Fe3+
Gibberella sp.
strong
Fe3+
Bispora sp.
-
5 mM Fe3+ and Mn2+ decrease enzyme activity to 70% and 89% of its initial activity
FeCl2
-
1 mM, 50% inhibition
FeCl3
-
1.0 mM, 46.2% inhibition
FeSO4
-
1.0 mM, 50.7% inhibition
FeSO4
69.5% residual acitivty at 10 mM
fructose
-
-
galactose
-
powerful inhibitor of alpha-Gal II, alpha-Gal III and alpha-Gal IV. 30% inhibition at 0.5 mM, nearly 100% inhibition at 40 mM
galactose
-
-
galactose
-
relative inhibition of 83.8%, effective inhibitor
galactostatin bisulfite
-
inhibits in vitro, increases enzyme activity in cultured Fabry fibroblasts. Binding is less strong than binding of 1-deoxygalactonojirimycin, and interaction with enzyme protein is mainly enthalpie-driven but entropy-driven factors may be involved. Inhibitor fits to the active-site pocket and undergoes hydrogen bonding with residues comprising the active-site pocket including the catalytic ones
galactostatin bisulfite
-
-
gamma-1,4-galactonolactone
-
-
-
glycerol 3-phosphate
-
-
Hg+
-
complete inactivation at 10 mM
Hg+
20.9% residual activity at 1 mM
Hg2+
1 mM, more than 95% inhibition
Hg2+
-
1 mM, 91% inhibition
Hg2+
-
1 mM, strong inhibition
Hg2+
1 mM, 98% inhibition
Hg2+
alpha-Gal II, 93% decrease of activity at 1 mM; alpha-Gal III, 87% decrease of activity at 1 mM
Hg2+
Rhizopus sp.
-
no activity at 1 mM
Hg2+
-
strong inhibitor
Hg2+
-
complete inhibition at 10 mM
Hg2+
complete inhibition at 10 mM
Hg2+
-
potent inhibitor
Hg2+
Gibberella sp.
strong
Hg2+
-
inactivation
Hg2+
Tachigali multijuga
-
-
Hg2+
strong inhibitor
Hg2+
-
inhibits enzymatic activity
Hg2+
-
completely inhibits activity
Hg2+
Bispora sp.
-
strongly inhibits AgalB activity
Hg2+
complete inhibition at 10 mM
HgCl2
-
1.0 mM, 88.2% inhibition
HgCl2
-
0.5 mM, complete inhibition, alpha-galactosidase 1; 2.0 mM, complete inhibition, alpha-galactosidase 2; 2.0 mM, complete inhibition, alpha-galactosidase 3
iodoacetamide
-
95.88% activity at 10 mM
iodoacetamide
-
98.87% activity at 10 mM
iodoacetic acid
-
-
K+
-
96.48% activity at 10 mM
KCl
-
2.0 M, 41.3% inhibition; 2.0 M, 74.6% inhibition, alpha-galactosidase 3; 2.0 M, 8.8% inhibition, alpha-galactosidase 1
L-arabinose
-
-
L-arabinose
-
-
L-ascorbic acid
-
-
lactose
-
12% inhibition at 2 mM
Li+
93.3% residual activity at 1 mM
Li+
Rhizopus sp.
-
96.7% activity at 1 mM
mannose
-
70% inhibition of alpha-Gal I at 40 mM
melibiose
-
with p-nitrophenyl alpha-D-galactopyranoside as substrate
melibiose
-
2 mM, 16% inhibition of enzyme P1, 36% inhibition of enzyme P2
melibiose
-
39.7% activity at 10 mM
melibiose
-
74.14% activity at 10 mM
melibiose
-
58% activity at 10 mM
melibiose
-
46% inhibition at 2 mM
melibiose
Tachigali multijuga
-
-
methyl alpha-D-galactopyranoside
-
15% inhibition at 2 mM
methyl beta-D-galactopyranoside
-
13% inhibition at 2 mM
Mg2+
92.6% residual activity at 1 mM
Mg2+
Rhizopus sp.
-
98.2% activity at 1 mM
Mg2+
-
94.15% activity at 10 mM
Mg2+
-
98% activity at 10 mM
Mg2+
93.4% residual activity at 10 mM
MgCl2
-
10 mM, 16.4% inhibition
Mn2+
91.5% residual activity at 1 mM
Mn2+
Rhizopus sp.
-
49.5% activity at 1 mM
Mn2+
complete inhibition at 10 mM
Mn2+
strong inhibitor
Mn2+
Bispora sp.
-
5 mM Fe3+ and Mn2+ decrease enzyme activity to 70% and 89% of its initial activity
myo-inositol
-
-
myo-inositol
-
-
myo-inositol
-
-
myo-inositol
-
-
myo-inositol
-
-
N-(N-benzyloxycarbonyl-epsilon-aminocaproyl)-alpha-D-galactopyranoside
Coffea sp.
-
-
N-acetyl-D-galactosamine
-
-
N-bromosuccinimide
-
complete inhibition at 0.02 mM, at pH 5.0
N-bromosuccinimide
-
almost complete inhibition
N-bromosuccinimide
-
100% inhibition
n-butyldeoxygalactonojirimycin
-
2000fold less effective than 1-deoxygalactonojirimycin
N-epsilon-Aminocaproyl-alpha-D-galactopyranoside
Coffea sp.
-
-
Na+
-
94.2% activity at 10 mM
Na+
-
96% activity at 10 mM
NaCl
-
2.0 M, 21.9% inhibition, alpha-galactosidase 1; 2.0 M, 46.7% inhibition, alpha-galactosidase 2; 2.0 M, 78.9% inhibition, alpha-galactosidase 2
NaN3
-
-
NEM
-
10 mM, 12.9% inhibition
Ni(2+)
Rhizopus sp.
-
92.7% activity at 1 mM
Ni2+
-
slight inhibition at 10 mM
Ni2+
complete inhibition at 10 mM
NiCl2
-
1.0 mM, 21.7% inhibition
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
alpha-Gal III shows less than 8% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20C for 1 h; alpha-Gal II shows less than 6% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20C for 1 h
p-chloromercuribenzoate
-
16% residual activity at 0.01 mM, at pH 5.8
p-hydroxymercuribenzoate
-
0.25 mM, 97.8% inhibition
p-nitrophenyl-alpha-D-galactoside
-
substrate inhibition above 4 mM, alpha-galactosidase 2; substrate inhibition above 4 mM, alpha-galactosidase 3
Paraoxon
-
-
PCMB
1 mM, 30C, 30 min, complete inhibition of wild-type enzyme, no inhibition of mutant enzyme C159A
PCMB
1 mM, 98% inhibition
PCMB
-
potent inhibitor
raffinose
-
with p-nitrophenyl alpha-D-galactopyranoside as substrate
raffinose
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
raffinose
-
80.60% activity at 10 mM
raffinose
-
81.73% activity at 10 mM
raffinose
-
93% activity at 10 mM
SDS
-
1 mM, 98% inhibition of enzyme P1, 37% inhibition of enzyme P2
SDS
Rhizopus sp.
-
no activity at 1 mM
SDS
-
46.9% activity at 10 mM
SDS
-
49.36% activity at 10 mM
SDS
-
complete inhibition at 10 mM
SDS
strong inhibitor
SDS
-
completely inhibits activity
SDS
Bispora sp.
-
strongly inhibits AgalB activity
SDS
7.6% residual acitivty at 10 mM
Sodium dodecyl sulfate
Gibberella sp.
strong
Sodium dodecyl sulfate
Tachigali multijuga
-
-
sodium taurocholate
-
-
stachyose
-
with p-nitrophenyl alpha-D-galactopyranoside as substrate
stachyose
-
95.73% activity at 10 mM
stachyose
-
87.69% activity at 10 mM
stachyose
-
88% activity at 10 mM
Woodward's reagent K
-
21% residual activity at 5 mM, at pH 6.0
Zn2+
-
alpha-galactosidase II
Zn2+
-
strong inhibition at 10 mM
Zn2+
78.3% residual activity at 1 mM
Zn2+
complete inhibition at 10 mM
ZnCl2
-
1.0 mM, 99.3% inhibition
MnCl2
-
1.0 mM, 18.4% inhibition
additional information
Rhizopus sp.
-
not inhibited by Na+ and Zn2+
-
additional information
-
inhibiting saccharides used at three concentrations, 10, 50, and 100 mM, no inhibitory effects of glucose, inositol, sucrose
-
additional information
-
not inhibited by D-lactose
-
additional information
-
not inhibited by sucrose, EDTA, and K+
-
additional information
D-galactose, L-arabinose, D-fucose, sucrose, EDTA, dithiothreitol, Mg2+ and Mo2+ have no inhibitory effect
-
additional information
-
not inhibited by 2-acetamido-2-deoxy-D-galactopyranose, D-galactosamine hydrochloride, D-glucose, 2-deoxy-D-glucose, 6-deoxy-D-glucose, 2-acetamido-2-deoxy-D-glucopyranose, D-glucosamine hydrochloride, saccharose, stachyose, D-fructose and D-arabinose
-
additional information
-
beta-mercaptoethanol (1.0 mM) and urea (1.0 M) do not inhibit the enzyme activity
-
additional information
-
not inhibitory: Mg2+, Ni2+, Ca2+, Co2+, Mn2+
-
additional information
Gibberella sp.
enzyme is strongly resistant to alpha-chymotrypsin, subtilisin A, and collagenase digestion
-
additional information
-
decrease of enzyme activity in mesocarp tissue during fruit development
-
additional information
not inhibited by EDTA, 2-mercaptoethanol
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-deoxygalactonojirimycin
-
3.8fold increase at 0.02 mM after 5 days
1-deoxygalactonojirimycin
-
acts as a chemical chaperone and enhances catalytic activity and protein expression in milder mutations associated with the atypical form of Fabry disease
1-deoxygalactonojirimycin
-
T-cells from normal controls resond with 28% increase in alpha-galactosidase activity to treatment with 1-deoxygalactonojirimycin. In cells from patients with Fabry disease, response depends on he mutantion and ranges from no increase to fully normal activity. In normal cells and in cells from patients responding more than 25%, an increase in the mature lysosomal form of enzyme is observed after treatment. In the group of intermediate respoders with increases of 7-25% in activity upon treatment, an increase in protein stain but incomplete processing of the enzyme to the mature form is detected
2-mercaptoethanol
-
107.8% activity at 10 mM
2-mercaptoethanol
-
required for enzymatic activity
6-deoxy-D-glucose
-
the enzyme is produced exclusively in the presence of the specific inducer 6-deoxy-D-glucose, also named quinovose
beta-mercaptoethanol
-
activity enhanced by 2fold
D-glucose
-
increase of activity
D-glucose
-
105.1% activity at 10 mM
D-sucrose
-
116.5% activity at 10 mM
dithiothreitol
or similar reducing agent, required
EDTA
-
105% activity at 10 mM
EDTA
16% increase in activity
EDTA
the activity of recombinant Aga-F78 is significantly enhanced in the presence of EDTA
EDTA
114.7% activity at 10 mM
iodoacetamide
-
104.3% activity at 10 mM
L-arabinose
-
the enzyme is strongly induced by L-arabinose
NAD+
strong activation
NADP+
slight activation
NH4+
23% increase in activity
Pb2+
Gibberella sp.
5 mM, significant enhancement of activity
microwave
microwave irradiation (300 W) significantly stimulates activity
-
additional information
-
D-galactose, melibiose, raffinose, stachyose, methyl alpha-D-galactopyranoside, 6-deoxy-D-galactose, D-glucose, 2-deoxy D-glucose, 6-O-tosyl-D-glucose, 6-chloro-6-deoxy-dglucose, D-arabinose, and D-xylose do not stimulate enzyme production
-
additional information
-
increase of enzyme activity in leaves during fruit development at day 4 after anthesis
-
additional information
Bispora sp.
-
a synergistic (3fold) increase in guar gum hydrolysis is observed when beta-mannanase Man5A from Bispora sp. MEY-1 and r-AgalB are combined
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.037
2,4-dinitrophenyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.135
2,4-dinitrophenyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.143
2,5-dinitrophenyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.243
2,5-dinitrophenyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.133
2-nitrophenyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.143
2-nitrophenyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.28
2-nitrophenyl alpha-D-galactoside
-
pH 5.0, 65C
0.086
3,4-dimethylphenyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.089
3,4-dimethylphenyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.096
3-nitrophenyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.14
3-nitrophenyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.104
4-bromophenyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.111
4-bromophenyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
2.9
4-methylumbelliferyl alpha-D-galactopyranoside
-
alpha-galactosidase A
2.9
4-methylumbelliferyl alpha-D-galactopyranoside
-
-
6.8
4-methylumbelliferyl alpha-D-galactopyranoside
-
alpha-galactosidase B
6.8
4-methylumbelliferyl alpha-D-galactopyranoside
-
-
0.45
4-methylumbelliferyl alpha-D-galactoside
-
-
0.5
4-methylumbelliferyl alpha-D-galactoside
-
50C, pH 4.5
2.2
4-methylumbelliferyl alpha-D-galactoside
-
wild-type enzyme
2.3
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, mutant enzyme E66D; pH 4.6, wild-type enzyme
2.8
4-methylumbelliferyl alpha-D-galactoside
-
mutant enzyme Q279E and R301Q
3.4
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, mutant enzyme S65A
3.5
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, mutant enzyme S65T
4
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, 37C, mutant M51I
4.5
4-methylumbelliferyl alpha-D-galactoside
-
pH 4.6, 37C, wild-type
1.8
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme Q279E, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
1.9
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme R301Q, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme R356W, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2.4
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme M296V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2.6
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme L166V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2.7
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme M296I, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
2.8
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme A20P, in 0.1 M sodium citrate buffer (pH 4.6) at 37C; mutant enzyme N215S, in 0.1 M sodium citrate buffer (pH 4.6) at 37C; wild type enzyme, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.1
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme E66Q, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.2
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme M72V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.4
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme A156V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C; mutant enzyme I91T, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.6
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme F113L, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
3.7
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme A97V, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
5.8
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme R112H, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
16.3
4-methylumbelliferyl-alpha-D-galactoside
-
mutant enzyme E59K, in 0.1 M sodium citrate buffer (pH 4.6) at 37C
0.21
4-nitrophenyl alpha-D-galactopyranoside
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
0.24
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
0.44
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
0.48
4-nitrophenyl alpha-D-galactopyranoside
-
pH 5.0, 40C
0.61
4-nitrophenyl alpha-D-galactopyranoside
-
pH 5.0, 40C
0.83
4-nitrophenyl alpha-D-galactopyranoside
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
0.84
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
1.33
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336D of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
2.2
4-nitrophenyl alpha-D-galactopyranoside
at pH 5.0 and 60C
8.7
4-nitrophenyl alpha-D-galactopyranoside
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
0.08
4-nitrophenyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.083
4-nitrophenyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.1
4-nitrophenyl alpha-D-galactoside
-
pH 5.0, 65C
0.19
4-nitrophenyl alpha-D-galactoside
mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
0.31
4-nitrophenyl alpha-D-galactoside
wild type enzyme, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
0.32
4-nitrophenyl alpha-D-galactoside
mutant enzyme E470Q, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
0.45
4-nitrophenyl alpha-D-galactoside
Tachigali multijuga
-
pH 5.0, 40C
0.51
4-nitrophenyl alpha-D-galactoside
-
-
0.2
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 40C
0.24
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant Q251W, pH 7.4, 30C
0.27
4-nitrophenyl-alpha-D-galactopyranoside
-
0.27
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 37C
0.29
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 4.5, 50C
0.32
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5.0, 55C
0.65
4-nitrophenyl-alpha-D-galactopyranoside
pH 6.0, 36C
0.66
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 40C
0.72
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5, 40C
1.06
4-nitrophenyl-alpha-D-galactopyranoside
Gibberella sp.
37C
1.4
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant Q251A, pH 7.4, 30C
2.47
4-nitrophenyl-alpha-D-galactopyranoside
-
pH 5.5, 45C
3.1
4-nitrophenyl-alpha-D-galactopyranoside
Bispora sp.
-
pH 5.5, 37C
3.7
4-nitrophenyl-alpha-D-galactopyranoside
-
mutant A41Y, pH 7.4, 30C
4.5
4-nitrophenyl-alpha-D-galactopyranoside
-
wild-type, pH 7.4, 30C
0.048
4-O-methylphenyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.088
4-O-methylphenyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.62
6-bromo-2-naphthyl alpha-D-galactoside
-
alpha-galactosidase II
1.8
6-bromo-2-naphthyl alpha-D-galactoside
-
alpha-galactosidase I
1
alpha-phenylgalactoside
Coffea sp.
-
alpha-galactosidase II
-
2
alpha-phenylgalactoside
Coffea sp.
-
alpha-galactosidase I
-
0.039
beta-naphthyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.225
beta-naphthyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
2
digalactosyl diacylglycerol
-
at 37C, pH 8.6
6.25
ethyl alpha-D-galactoside
-
-
8
ethyl alpha-D-galactoside
-
alpha-galactosidase II
8.93
ethyl alpha-D-galactoside
-
alpha-galactosidase I
0.28
Galabiosylceramide
-
-
0.13
galactinol
-
alpha-galactosidase I
0.69
galactinol
-
alpha-galactosidase II
44
galactomannan with galactose/mannose ratio 1:1.72
-
pH 5.0, 50C
-
34
galactomannan with galactose/mannose ratio 1:2.4
-
pH 5.0, 50C
-
3.7
globopentaose
-
-
9.1
globotriose
-
-
0.18
globtriglycosylceramide
-
-
-
0.83
m-chlorophenyl alpha-D-galactoside
-
alpha-galactosidase I
1.17
m-chlorophenyl alpha-D-galactoside
-
alpha-galactosidase II
8.33
m-chlorophenyl alpha-D-galactoside
-
-
1.38
m-cresyl alpha-D-galactoside
-
alpha-galactosidase I
2
m-cresyl alpha-D-galactoside
-
alpha-galactosidase II
8.33
m-cresyl alpha-D-galactoside
-
-
1.57
m-nitrophenyl alpha-D-galactoside
-
-
2
m-nitrophenyl alpha-D-galactoside
-
-
2.5
m-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
10
m-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
0.72
m-nitrophenyl-alpha-D-galactopyranoside
-
-
1.1
m-nitrophenyl-alpha-D-galactopyranoside
-
-
0.00135
melibiose
-
-
0.39
melibiose
-
pH 4.5, 50C, isoenzyme alpha-Gal II
0.77
melibiose
-
alpha-galactosidase II
0.8
melibiose
-
-
0.96
melibiose
-
alpha-galactosidase I
1
melibiose
-
pH 4.5, 50C, isoenzyme alpha-Gal I
1.3
melibiose
-
-
1.4
melibiose
pH 6.0, 36C; pH 6, 36C
1.5
melibiose
-
-
1.7
melibiose
-
30C, pH 5.8
1.75
melibiose
Gibberella sp.
37C
1.92
melibiose
-
pH 5, 40C
2
melibiose
Corynebacterium murisepticum
-
-
2.12
melibiose
-
pH 5.0, 55C, intracellular enzyme
2.14
melibiose
-
pH 5.5, 37C
2.24
melibiose
-
-
2.3
melibiose
-
alpha-galactosidase II
2.33
melibiose
-
-
2.4
melibiose
-
50C, pH 4.5
3
melibiose
-
larger form
3.5
melibiose
-
smaller form
3.7
melibiose
-
alpha-galactosidase I
3.8
melibiose
-
alpha-galactosidase II
5.37
melibiose
Tachigali multijuga
-
pH 5.0, 40C
5.53
melibiose
-
enzyme form P2
5.8
melibiose
-
mutant A41Y, pH 7.4, 30C
6.3
melibiose
-
30C, pH 5.0
7.39
melibiose
-
pH 5, 40C
7.9
melibiose
-
-
11.2
melibiose
-
wild-type, pH 7.4, 30C
12
melibiose
-
pH 5, 37C
12.58
melibiose
-
pH 5.5, 45C
15.11
melibiose
-
pH 7, 98C
19
melibiose
-
alpha-galactosidase I
20.14
melibiose
-
pH 5, 40C
20.8
melibiose
-
alpha-galactosidase I
24
melibiose
-
-
25.4
melibiose
-
at 37C, pH 8.6
29.4
melibiose
at pH 5.0 and 60C
33
melibiose
Rhizopus sp.
-
in 0.1 M McIlvaine buffer (pH 4.8) at 37C
45
melibiose
-
alpha-galactosidase II
254
melibiose
-
mutant Q251A, pH 7.4, 30C
5.3
methyl alpha-D-galactoside
-
alpha-galactosidase I
7.13
methyl alpha-D-galactoside
-
alpha-galactosidase I
8.4
methyl alpha-D-galactoside
-
alpha-galactosidase II
10.9
methyl alpha-D-galactoside
-
-
14.3
methyl alpha-D-galactoside
-
alpha-galactosidase II
5.88
n-propyl alpha-D-galactoside
-
alpha-galactosidase II
6.13
n-propyl alpha-D-galactoside
-
alpha-galactosidase I
6.25
n-propyl alpha-D-galactoside
-
-
0.78
o-cresyl alpha-D-galactoside
-
alpha-galactosidase II
1.33
o-cresyl alpha-D-galactoside
-
alpha-galactosidase I
4.54
o-cresyl alpha-D-galactoside
-
-
8.8
o-nitrophenyl alpha-D-fucopyranoside
-
-
0.33
o-nitrophenyl alpha-D-galactoside
-
-
0.69
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
1.14
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
1.3
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase III
1.4
o-nitrophenyl alpha-D-galactoside
-
-
2.3
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
3.8
o-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
0.26
o-nitrophenyl-alpha-D-galactopyranose
-
-
0.84
o-nitrophenyl-alpha-D-galactopyranoside
-
-
2.27
o-nitrophenyl-alpha-D-galactopyranoside
-
-
1.3
o-nitrophenyl-alpha-N-acetyl-galactosaminide
-
alpha-galactosidase B
0.2
o-nitrophenyl-beta-D-galactopyranose
-
alpha-galactosidase II
0.3
o-nitrophenyl-beta-D-galactopyranose
-
alpha-galactosidase II
0.87
p-aminophenyl alpha-D-galactoside
-
alpha-galactosidase II
0.95
p-aminophenyl alpha-D-galactoside
-
alpha-galactosidase I
1
p-cresyl alpha-D-galactoside
-
alpha-galactosidase II
-
1.54
p-cresyl alpha-D-galactoside
-
alpha-galactosidase I
-
4.76
p-cresyl alpha-D-galactoside
-
-
-
4.76
p-nitrophenyl alpha-D-fucoside
-
alpha-galactosidase I
5.88
p-nitrophenyl alpha-D-fucoside
-
alpha-galactosidase II
0.075
p-nitrophenyl alpha-D-galactopyranoside
-
pH 5, 75C
0.085
p-nitrophenyl alpha-D-galactopyranoside
in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80C
0.22
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50C, isoenzyme alpha-Gal II
0.221
p-nitrophenyl alpha-D-galactopyranoside
-
-
0.24
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50C, isoenzyme alpha-Gal IV
0.27
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50C, isoenzyme alpha-Gal III
0.29 - 0.35
p-nitrophenyl alpha-D-galactopyranoside
-
-
0.4
p-nitrophenyl alpha-D-galactopyranoside
-
pH 6.0, 40C
0.52
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-b2
0.54
p-nitrophenyl alpha-D-galactopyranoside
-
at 30C
0.69
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-m2
0.74
p-nitrophenyl alpha-D-galactopyranoside
-
pH 7, 98C
0.76
p-nitrophenyl alpha-D-galactopyranoside
-
enzyme form P2
1.1
p-nitrophenyl alpha-D-galactopyranoside
-
at 55C
1.13
p-nitrophenyl alpha-D-galactopyranoside
-
at 55C, in 50 mM citrate buffer (pH 4.2)
1.4
p-nitrophenyl alpha-D-galactopyranoside
-
pH 4.5, 50C, isoenzyme alpha-Gal I
1.55
p-nitrophenyl alpha-D-galactopyranoside
-
enzyme form P1
1.89
p-nitrophenyl alpha-D-galactopyranoside
-
-
2.9
p-nitrophenyl alpha-D-galactopyranoside
Rhizopus sp.
-
in 0.1 M McIlvaine buffer (pH 4.8) at 37C
3.2
p-nitrophenyl alpha-D-galactopyranoside
-
30C, pH 5.0
4.7
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-b1
4.7
p-nitrophenyl alpha-D-galactopyranoside
pH 6.0, 70C
4.72
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-m1
7.59
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-m3
7.9
p-nitrophenyl alpha-D-galactopyranoside
-
isoenzyme AgaS-b3
12
p-nitrophenyl alpha-D-galactopyranoside
-
30C, pH 5.8
0.28
p-nitrophenyl alpha-D-galactose
-
alpha-galactosidase I
0.56
p-nitrophenyl alpha-D-galactose
-
alpha-galactosidase II
0.08
p-nitrophenyl alpha-D-galactoside
-
-
0.12
p-nitrophenyl alpha-D-galactoside
-
-
0.15
p-nitrophenyl alpha-D-galactoside
-
tetramer
0.22
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
0.22
p-nitrophenyl alpha-D-galactoside
-
larger form
0.26
p-nitrophenyl alpha-D-galactoside
-
-
0.31
p-nitrophenyl alpha-D-galactoside
-
-
0.32
p-nitrophenyl alpha-D-galactoside
-
smaller form
0.35
p-nitrophenyl alpha-D-galactoside
-
alpha-D-galactosidase A
0.38
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
0.38
p-nitrophenyl alpha-D-galactoside
-
-
0.39
p-nitrophenyl alpha-D-galactoside
-
monomer
0.42
p-nitrophenyl alpha-D-galactoside
-
-
0.45
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II
0.47
p-nitrophenyl alpha-D-galactoside
-
-
0.53
p-nitrophenyl alpha-D-galactoside
-
-
0.54
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
0.71
p-nitrophenyl alpha-D-galactoside
-
-
0.76
p-nitrophenyl alpha-D-galactoside
-
-
0.8
p-nitrophenyl alpha-D-galactoside
-
-
1
p-nitrophenyl alpha-D-galactoside
-
-
1
p-nitrophenyl alpha-D-galactoside
-
-
1.2
p-nitrophenyl alpha-D-galactoside
-
-
1.5
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase I
2.2
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II2
5.3
p-nitrophenyl alpha-D-galactoside
-
alpha-galactosidase II1
6
p-nitrophenyl alpha-D-galactoside
-
-
12.5
p-nitrophenyl beta-L-arabinoside
-
alpha-galactosidase II
14.3
p-nitrophenyl beta-L-arabinoside
-
alpha-galactosidase I
33.3
p-nitrophenyl beta-L-arabinoside
-
-
0.16
p-nitrophenyl-alpha-D-galactopyranose
-
-
0.17
p-nitrophenyl-alpha-D-galactopyranose
Corynebacterium murisepticum
-
-
0.23
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase II, pH 6
0.32
p-nitrophenyl-alpha-D-galactopyranoside
-
-
0.47
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase I, pH 7
0.53
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase II, pH 7
0.59
p-nitrophenyl-alpha-D-galactopyranoside
-
alpha-galactosidase I, pH 6
0.75
p-nitrophenyl-alpha-D-galactopyranoside
-
-
0.83
p-nitrophenyl-alpha-D-galactopyranoside
-
-
1.46
p-nitrophenyl-alpha-D-galactopyranoside
-
-
0.26
p-nitrophenyl-alpha-D-galactoside
-
cell wall alpha-galactosidase I
0.29
p-nitrophenyl-alpha-D-galactoside
-
protein bodies alpha-galactosidase I
0.31
p-nitrophenyl-alpha-D-galactoside
-
cell wall alpha-galactosidase II
0.65
p-nitrophenyl-alpha-D-galactoside
pH 6, 36C
1.09
p-nitrophenyl-alpha-D-galactoside
-
alpha-galactosidase 3
1.35
p-nitrophenyl-alpha-D-galactoside
-
alpha-galactosidase 2
1.36
p-nitrophenyl-alpha-D-galactoside
-
alpha-galactosidase 1
0.2
p-nitrophenyl-beta-D-galactopyranose
-
alpha-galactosidase II
0.4
p-nitrophenyl-beta-D-galactopyranose
-
alpha-galactosidase II
37.4
p-nitrophenyl-beta-L-arabinopyranoside
in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80C
0.176
phenyl alpha-D-galactoside
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.324
phenyl alpha-D-galactoside
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
1.11
phenyl alpha-D-galactoside
-
alpha-galactosidase I
1.25
phenyl alpha-D-galactoside
-
alpha-galactosidase II
5
phenyl alpha-D-galactoside
-
-
6.9
planteose
-
-
0.42
raffinose
-
pH 5.0, 65C
0.49
raffinose
-
pH 5.0, 40C
0.5
raffinose
-
-
0.66
raffinose
-
pH 5.5, 37C
1.6
raffinose
-
alpha-galactosidase II2
1.61
raffinose
-
at 55C, in 50 mM citrate buffer (pH 4.2)
1.8
raffinose
-
alpha-galactosidase I
2 - 3
raffinose
-
pH 5.0, 40C
2.1
raffinose
-
-
2.1
raffinose
-
pH 5, 75C
2.16
raffinose
-
-
2.7
raffinose
-
alpha-galactosidase II
3.65
raffinose
-
-
3.8
raffinose
-
-
3.8
raffinose
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
4
raffinose
-
alpha-galactosidase I
4.4
raffinose
-
30C, pH 5.0
4.6
raffinose
-
alpha-galactosidase I
4.8
raffinose
-
30C, pH 5.8
5
raffinose
-
alpha-galactosidase II
5
raffinose
-
alpha-galactosidase II1
5
raffinose
-
smaller form
5.32
raffinose
-
pH 7, 98C
5.34
raffinose
-
enzyme form P2
5.9
raffinose
-
alpha-galactosidase II
6.5
raffinose
-
larger form
11.3
raffinose
-
50C, pH 4.5
11.8
raffinose
-
-
12.5
raffinose
-
-
12.6
raffinose
-
-
15
raffinose
-
pH 5, 37C
18
raffinose
-
at 37C, pH 8.6
24.1
raffinose
-
-
25.9
raffinose
-
-
26.3
raffinose
-
alpha-galactosidase III
27.7
raffinose
-
alpha-galactosidase I
27.93
raffinose
-
pH 5, 40C
32.8
raffinose
-
pH 5.0, 55C, intracellular enzyme
32.99
raffinose
-
pH 5, 40C
36.4
raffinose
-
-
36.6
raffinose
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
39.62
raffinose
Tachigali multijuga
-
pH 5.0, 40C
40
raffinose
-
-
54.1
raffinose
-
wild-type, pH 7.4, 30C
54.26
raffinose
Gibberella sp.
37C
71.4
raffinose
-
alpha-galactosidase II
80
raffinose
-
-
87.9
raffinose
-
mutant A41Y, pH 7.4, 30C
98.1
raffinose
-
alpha-galactosidase I
105
raffinose
-
pH 7.5, temperature not specified in the publication
200
raffinose
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
205
raffinose
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
250
raffinose
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
252
raffinose
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
0.22
stachyose
-
pH 5.0, 40C
0.3
stachyose
-
alpha-galactosidase II
0.33
stachyose
-
pH 5.0, 65C
1.15
stachyose
-
-
1.17
stachyose
-
at 55C, in 50 mM citrate buffer (pH 4.2)
1.8
stachyose
-
-
2.4
stachyose
-
alpha-galactosidase I
3.8
stachyose
-
alpha-galactosidase II
3.8
stachyose
-
30C, pH 5.0
4.5
stachyose
-
-
5.26
stachyose
-
alpha-galactosidase II
7.2
stachyose
-
30C, pH 5.8
7.5
stachyose
-
alpha-galactosidase I
7.8
stachyose
-
at 37C, pH 8.6
8.23
stachyose
Gibberella sp.
37C
8.5
stachyose
-
alpha-galactosidase I
10
stachyose
-
larger form
10.8
stachyose
-
pH 5.0, 55C, intracellular enzyme
10.94
stachyose
-
pH 5, 40C
11
stachyose
-
alpha-galactosidase I
13
stachyose
-
-
14.76
stachyose
-
pH 5.5, 45C
15
stachyose
-
alpha-galactosidase II1
21
stachyose
-
pH 5.0, 40C
30
stachyose
-
smaller form
33.3
stachyose
-
alpha-galactosidase III
35.5
stachyose
-
alpha-galactosidase I
48.8
stachyose
Tachigali multijuga
-
pH 5.0, 40C
54.74
stachyose
-
pH 5, 40C
72
stachyose
-
alpha-galactosidase II
4.2
verbascose
-
at 37C, pH 8.6
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.085
2,4-dinitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.41
2,4-dinitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
12.23
2,4-dinitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.042
2,5-dinitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
11.2
2,5-dinitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.035
2-nitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.52
2-nitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
13.67
2-nitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.01
3,4-dimethylphenyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.052 - 2.1
3,4-dimethylphenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
10.39
3,4-dimethylphenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.042
3-nitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
10.5
3-nitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.016
4-bromophenyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
12.58
4-bromophenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
49.8
4-methylumbelliferyl alpha-D-galactoside
Thermomyces lanuginosus
-
50C, pH 4.5
0.97
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336D of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
7
4-nitrophenyl alpha-D-galactopyranoside
Penicillium canescens
-
pH 5.0, 40C
7.9
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
38.2
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
48
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
105.6
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
375
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
512
4-nitrophenyl alpha-D-galactopyranoside
Sphingomonas sp.
M4GRS1
at pH 5.0 and 60C
1800
4-nitrophenyl alpha-D-galactopyranoside
Penicillium canescens
-
pH 5.0, 40C
2382
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
0.00218
4-nitrophenyl alpha-D-galactoside
Bacteroides thetaiotaomicron
Q8A6L0
mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
0.03
4-nitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.0684
4-nitrophenyl alpha-D-galactoside
Bacteroides thetaiotaomicron
Q8A6L0
mutant enzyme E470Q, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
8.58
4-nitrophenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
244
4-nitrophenyl alpha-D-galactoside
Bacteroides thetaiotaomicron
Q8A6L0
wild type enzyme, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
3279
4-nitrophenyl alpha-D-galactoside
Yersinia pestis
-
-
0.12
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 4.5, 37C
0.23
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 9.0, 37C
0.3
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 6.7, 37C
0.62
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 8.7, 37C
1.05
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 7.1, 37C
1.37
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 8.4, 37C
1.68
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 7.5, 37C
1.75
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 8.0, 37C
2.07
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 7.8, 37C
3.29
4-nitrophenyl-alpha-D-galactopyranoside
Debaryomyces hansenii
-
pH 5.0, 55C, intracellular enzyme
16
4-nitrophenyl-alpha-D-galactopyranoside
Saccharomyces cerevisiae
-
mutant Q251W, pH 7.4, 30C
90
4-nitrophenyl-alpha-D-galactopyranoside
Saccharomyces cerevisiae
-
mutant A41Y, pH 7.4, 30C
170
4-nitrophenyl-alpha-D-galactopyranoside
Saccharomyces cerevisiae
-
mutant Q251A, pH 7.4, 30C
286
4-nitrophenyl-alpha-D-galactopyranoside
Saccharomyces cerevisiae
-
wild-type, pH 7.4, 30C
654.6
4-nitrophenyl-alpha-D-galactopyranoside
Flavobacterium sp.
-
pH 5.5, 45C
1278
4-nitrophenyl-alpha-D-galactopyranoside
Aspergillus nidulans
-
pH 5, 37C
0.01
4-O-methylphenyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.03 - 0.55
4-O-methylphenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
6.69
4-O-methylphenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.021
beta-naphthyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
6.26
beta-naphthyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
7
galactomannan with galactose/mannose ratio 1:1.72
Penicillium canescens
-
pH 5.0, 50C
-
10
galactomannan with galactose/mannose ratio 1:2.4
Penicillium canescens
-
pH 5.0, 50C
-
0.822
m-nitrophenyl-alpha-D-galactopyranoside
Phaseolus vulgaris
-
-
0.03
melibiose
Debaryomyces hansenii
-
pH 5.0, 55C, intracellular enzyme
2.3
melibiose
Thermomyces lanuginosus
-
50C, pH 4.5
2.33
melibiose
Flavobacterium sp.
-
pH 5.5, 45C
113
melibiose
Saccharomyces cerevisiae
-
mutant Q251A, pH 7.4, 30C
131
melibiose
Saccharomyces cerevisiae
-
mutant A41Y, pH 7.4, 30C
193
melibiose
Saccharomyces cerevisiae
-
wild-type, pH 7.4, 30C
234.2
melibiose
Thermotoga neapolitana
-
pH 7, 98C
448
melibiose
Sphingomonas sp.
M4GRS1
at pH 5.0 and 60C
1067
melibiose
Aspergillus nidulans
-
pH 5, 37C
0.238
o-nitrophenyl-alpha-D-galactopyranoside
Phaseolus vulgaris
-
-
176
p-nitrophenyl alpha-D-galactopyranoside
Thermotoga maritima
-
pH 5, 75C
302.9
p-nitrophenyl alpha-D-galactopyranoside
Thermotoga neapolitana
-
pH 7, 98C
3914
p-nitrophenyl alpha-D-galactopyranoside
Thermomyces lanuginosus
-
at 55C, in 50 mM citrate buffer (pH 4.2)
0.027
phenyl alpha-D-galactoside
Thermotoga maritima
O33835
mutant enzyme D327G, in 200 mM sodium citrate buffer (pH 5.0), at 37C
5.71
phenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
0.89
raffinose
Penicillium canescens
-
pH 5.0, 40C
4.12
raffinose
Debaryomyces hansenii
-
pH 5.0, 55C, intracellular enzyme
5.2
raffinose
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
10.3
raffinose
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
32.4
raffinose
Thermomyces lanuginosus
-
50C, pH 4.5
46.8
raffinose
Saccharomyces cerevisiae
-
wild-type, pH 7.4, 30C
48.1
raffinose
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
91
raffinose
Saccharomyces cerevisiae
-
mutant A41Y, pH 7.4, 30C
109
raffinose
Thermotoga maritima
-
pH 5, 75C
157.3
raffinose
Thermotoga neapolitana
-
pH 7, 98C
180
raffinose
Geobacillus stearothermophilus
Q9ALJ4
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
586
raffinose
Aspergillus nidulans
-
pH 5, 37C
832
raffinose
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
1100
raffinose
Penicillium canescens
-
pH 5.0, 40C
1205
raffinose
Geobacillus stearothermophilus
Q9ALJ4
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
6946
raffinose
Thermomyces lanuginosus
-
at 55C, in 50 mM citrate buffer (pH 4.2)
0.57
stachyose
Penicillium canescens
-
pH 5.0, 40C
6.05
stachyose
Debaryomyces hansenii
-
pH 5.0, 55C, intracellular enzyme
42.04
stachyose
Flavobacterium sp.
-
pH 5.5, 45C
1250
stachyose
Penicillium canescens
-
pH 5.0, 40C
7659
stachyose
Thermomyces lanuginosus
-
at 55C, in 50 mM citrate buffer (pH 4.2)
3.61
p-nitrophenyl-alpha-D-galactopyranoside
Phaseolus vulgaris
-
-
additional information
phenyl alpha-D-galactoside
Thermotoga maritima
O33835
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.7
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336D of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
9797
5.5
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
9797
17.9
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
9797
45.5
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
9797
502
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
9797
1562
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
9797
2870
4-nitrophenyl alpha-D-galactopyranoside
Geobacillus stearothermophilus
Q9ALJ4
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
9797
0.0115
4-nitrophenyl alpha-D-galactoside
Bacteroides thetaiotaomicron
Q8A6L0
mutant enzyme D415N, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
4719
0.213
4-nitrophenyl alpha-D-galactoside
Bacteroides thetaiotaomicron
Q8A6L0
mutant enzyme E470Q, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
4719
778
4-nitrophenyl alpha-D-galactoside
Bacteroides thetaiotaomicron
Q8A6L0
wild type enzyme, in 40 mM sodium phosphate buffer, pH 6.0, at 35C
4719
0.091
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 4.5, 37C
2176
0.81
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 6.7, 37C
2176
0.96
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 9.0, 37C
2176
2.7
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 7.1, 37C
2176
5
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 8.7, 37C
2176
5.8
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 7.5, 37C
2176
8.9
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 8.0, 37C
2176
9.9
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 7.8, 37C
2176
15.6
4-nitrophenyl-alpha-D-galactopyranoside
Citrobacter freundii
-
pH 8.4, 37C
2176
24
4-nitrophenyl-alpha-D-galactopyranoside
Saccharomyces cerevisiae
-
mutant A41Y, pH 7.4, 30C
2176
63.5
4-nitrophenyl-alpha-D-galactopyranoside
Saccharomyces cerevisiae
-
wild-type, pH 7.4, 30C
2176
66
4-nitrophenyl-alpha-D-galactopyranoside
Saccharomyces cerevisiae
-
mutant Q251W, pH 7.4, 30C
2176
121
4-nitrophenyl-alpha-D-galactopyranoside
Saccharomyces cerevisiae
-
mutant Q251A, pH 7.4, 30C
2176
4730
4-nitrophenyl-alpha-D-galactopyranoside
Aspergillus nidulans
-
pH 5, 37C
2176
0.45
melibiose
Saccharomyces cerevisiae
-
mutant Q251A, pH 7.4, 30C
606
17
melibiose
Saccharomyces cerevisiae
-
wild-type, pH 7.4, 30C
606
22
melibiose
Saccharomyces cerevisiae
-
mutant A41Y, pH 7.4, 30C
606
88.9
melibiose
Aspergillus nidulans
-
pH 5, 37C
606
0.05
raffinose
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336N of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
432
0.14
raffinose
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336S of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
432
0.19
raffinose
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336A of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
432
0.8
raffinose
Saccharomyces cerevisiae
-
mutant A41Y, pH 7.4, 30C
432
0.9
raffinose
Saccharomyces cerevisiae
-
wild-type, pH 7.4, 30C
432
3.3
raffinose
Geobacillus stearothermophilus
Q9ALJ4
mutant enzyme W336F of isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
432
6
raffinose
Geobacillus stearothermophilus
Q9ALJ4
wild type enzyme isoform AgaB, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
432
39.1
raffinose
Aspergillus nidulans
-
pH 5, 37C
432
64.3
raffinose
Geobacillus stearothermophilus
Q9ALJ4
wild type enzyme isoform AgaA, in 100 mM potassium phosphate buffer, pH 6.5, at 25C
432
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.038
1-deoxygalactonojirimycin
-
pH 4.6, 37C, wild-type
0.047
1-deoxygalactonojirimycin
-
pH 4.6, 37C, mutant M51I
0.25
alpha-D-galactopyranosyl azide
-
at 30C
0.38
D-galactose
-
at 30C
0.7
D-galactose
-
pH 5.0, 55C, intracellular enzyme
2.74
D-galactose
Tachigali multijuga
-
pH 5.0, 40C
3.5
D-galactose
-
30C, pH 5.0
5.6
D-galactose
-
30C, pH 5.8
16.25
D-galactose
-
-
189
D-galactose
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
0.65
galactose
-
enzyme form P2
2.77
galactose
-
pH 4.5, 50C
0.095
galactostatin bisulfite
-
pH 4.6, 37C, wild-type
0.122
galactostatin bisulfite
-
pH 4.6, 37C, mutant M51I
9.2
gamma-1,4-galactonolactone
-
30C, pH 5.0
-
43.1
gamma-1,4-galactonolactone
-
30C, pH 5.8
-
7.7
melibiose
-
30C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
10.3
melibiose
-
30C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
9
raffinose
-
30C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
13.1
raffinose
-
30C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
25.4
raffinose
-
competitive with p-nitrophenyl alpha-D-galactopyranoside
6.6
stachyose
-
30C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
11.6
stachyose
-
30C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate
9.6
Tris
-
30C, pH 5.0
12
Tris
-
30C, pH 5.8
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6.9
(2S,3R,4R,5S)-2-(hydroxymethyl)-5-[(methylamino)methyl]pyrrolidine-3,4-diol
Coffea arabica
-
-
8.1
(2S,3R,4R,5S)-2-[(ethylamino)methyl]-5-(hydroxymethyl)pyrrolidine-3,4-diol
Coffea arabica
-
-
0.000048
1-deoxygalactonojirimycin
Homo sapiens
-
IC50 48 nM with mutant enzyme S65A
0.000058
1-deoxygalactonojirimycin
Homo sapiens
-
IC50 58 nM with mutant enzyme S65D
0.000059
1-deoxygalactonojirimycin
Homo sapiens
-
IC50 59 nM with wild-type enzyme
0.000063
1-deoxygalactonojirimycin
Homo sapiens
-
IC50: 63 nM with mutant enzyme S65T
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.000833
-
value about, grown under low temperature for at least 3 days
0.00167
-
value about, grown under normal temperature for at least 3 days
0.00167
-
value about, leaf, 12 to 20 days after anthesis
0.002083
-
value about, leaf, 8 days after anthesis
0.00375
-
value about, cold-sensitve cultivar, Guonong No. 41
0.004167
-
value about, cold-tolerant cultivar, Guonong No. 25
0.004167
-
value about, leaf, 2 days before anthesis, day of anthesis and 4 days after anthesis; value about, mesocarp tissue, 20 days after anthesis
0.0067
-
value about, mesocarp tissue, 16 days after anthesis
0.0083
-
value about, mesocarp tissue, 12 days after anthesis
0.0125
-
value about, mesocarp tissue, day of anthesis and 8 days after anthesis
0.015
-
value about, mesocarp tissue, 2 days before anthesis
0.02
-
D-glucose
0.02083
-
value about, mesocarp tissue, 4 days after anthesis
0.052
-
-
0.08
-
D-mannose
0.11
crude extract, using 4-nitrophenyl alpha-D-galactopyranoside as substrate, in 0.1 M Na+-phosphate buffer (pH 7.3), 0.15 M NaCl at 20C
0.13
-
D-galactose
0.18
crude cell extract, in 50 mM citric acid-Na2HPO4 buffer (pH 5.0) at 80C
0.21
cell-free extract
0.26
-
melibiose
0.3
-
raffinose
0.31
alpha-Gal III, crude extract
0.41
-
guar gum
0.42
-
stachyose
1.03
-
culture filtrate
1.2
Rhizopus sp.
-
crude filtrate
1.7
with raffinose as substrate, at pH 5.0 and 60C
2.02
-
crude extract, in 0.1 M sodium acetate buffer, pH 5.0, at 40C
2.1
-
alpha-galactosidase 3
2.25
alpha-Gal II, crude extract
2.3
-
alpha-galactosidase I
2.8
with cottonseed meal as substrate, at pH 5.0 and 60C
3.33
-
crude extract, in 0.1 M sodium acetate buffer, pH 4.0, at 40C
3.5
-
pH 5.0, 50C, substrate galactomannan with galactose/mannose ratio 1:1.72