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Results 1 - 10 of 14 > >>
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EC Number Protein Variants Commentary Reference
Display the reaction diagram Show all sequences 3.2.1.197D142N no activity -, 738663
Display the reaction diagram Show all sequences 3.2.1.197D363A no activity 738663
Display the reaction diagram Show all sequences 3.2.1.197E141Q no activity 738663
Display the reaction diagram Show all sequences 3.2.1.197E224A 1.9% of wild-type activity. Glutamic acid residues E265 and E224 are critical for the hydrolysis of beta-1,2-mannotriose. The residues are not conserved among GH130 phosphorylases and are predicted to assist the nucleophilic attack of a water molecule in the hydrolysis of the beta-D-mannosidic bond -, 738244
Display the reaction diagram Show all sequences 3.2.1.197E227Q 32% of wild-type activity 738663
Display the reaction diagram Show all sequences 3.2.1.197E227Q/E268Q no activity 738663
Display the reaction diagram Show all sequences 3.2.1.197E265A 0.04% of wild-type activity. Glutamic acid residues E265 and E224 are critical for the hydrolysis of beta-1,2-mannotriose. The residues are not conserved among GH130 phosphorylases and are predicted to assist the nucleophilic attack of a water molecule in the hydrolysis of the beta-D-mannosidic bond -, 738244
Display the reaction diagram Show all sequences 3.2.1.197E268A 5% of wild-type activity -, 738663
Display the reaction diagram Show all sequences 3.2.1.197E268Q 4% of wild-type activity -, 738663
Display the reaction diagram Show all sequences 3.2.1.197F344A 31% of wild-type activity 738663
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