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IUBMB CommentsThe enzyme, characterized from multiple bacterial species, catalyses the hydrolysis of terminal, non-reducing D-mannose residues from beta-1,2-mannotriose and beta-1,2-mannobiose. The mechanism involves anomeric inversion, resulting in the release of alpha-D-mannopyranose. Activity with beta-1,2-mannotriose or higher oligosaccharides is higher than that with beta-1,2-mannobiose.
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beta-1,2-D-mannoside mannohydrolase
The enzyme, characterized from multiple bacterial species, catalyses the hydrolysis of terminal, non-reducing D-mannose residues from beta-1,2-mannotriose and beta-1,2-mannobiose. The mechanism involves anomeric inversion, resulting in the release of alpha-D-mannopyranose. Activity with beta-1,2-mannotriose or higher oligosaccharides is higher than that with beta-1,2-mannobiose.
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beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranose + alpha-D-mannopyranose
Candida mannan + H2O
? + alpha-D-mannopyranose
additional information
?
-
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
-
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
-
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
i.e. beta-1,2-mannotetraose
-
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
i.e. beta-1,2-mannotetraose
-
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
i.e. beta-1,2-mannotriose
enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
i.e. beta-1,2-mannotriose
enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration
-
?
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranose + alpha-D-mannopyranose
-
-
-
?
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranose + alpha-D-mannopyranose
-
-
-
?
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranose + alpha-D-mannopyranose
i.e. beta-1,2-mannobiose
-
-
?
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranose + alpha-D-mannopyranose
i.e. beta-1,2-mannobiose
-
-
?
Candida mannan + H2O
? + alpha-D-mannopyranose
-
-
-
?
Candida mannan + H2O
? + alpha-D-mannopyranose
-
-
-
?
Candida mannan + H2O
? + alpha-D-mannopyranose
-
-
-
?
Candida mannan + H2O
? + alpha-D-mannopyranose
-
-
-
?
additional information
?
-
enzyme is a beta-mannosidase that hydrolyzes beta-1,2-mannosidic linkages through an inverting mechanism
-
-
?
additional information
?
-
enzyme is a beta-mannosidase that hydrolyzes beta-1,2-mannosidic linkages through an inverting mechanism
-
-
?
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beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration
-
?
beta-D-mannopyranosyl-(1->2)-beta-D-mannopyranosyl-(1->2)-D-mannopyranose + H2O
beta-D-mannopyranosyl-(1->2)-D-mannopyranose + alpha-D-mannopyranose
-
enzyme releases alpha-D-mannose from beta-1,2-mannooligosaccharides with an inversion of the anomeric configuration
-
?
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E227Q
32% of wild-type activity
E268A
5% of wild-type activity
E268Q
4% of wild-type activity
F344A
31% of wild-type activity
N74A
15% of wild-type activity
R89A
25% of wild-type activity
Y302A
6% of wild-type activity
E268A
-
5% of wild-type activity
-
E268Q
-
4% of wild-type activity
-
N74A
-
15% of wild-type activity
-
R89A
-
25% of wild-type activity
-
E224A
1.9% of wild-type activity. Glutamic acid residues E265 and E224 are critical for the hydrolysis of beta-1,2-mannotriose. The residues are not conserved among GH130 phosphorylases and are predicted to assist the nucleophilic attack of a water molecule in the hydrolysis of the beta-D-mannosidic bond
E265A
0.04% of wild-type activity. Glutamic acid residues E265 and E224 are critical for the hydrolysis of beta-1,2-mannotriose. The residues are not conserved among GH130 phosphorylases and are predicted to assist the nucleophilic attack of a water molecule in the hydrolysis of the beta-D-mannosidic bond
E224A
-
1.9% of wild-type activity. Glutamic acid residues E265 and E224 are critical for the hydrolysis of beta-1,2-mannotriose. The residues are not conserved among GH130 phosphorylases and are predicted to assist the nucleophilic attack of a water molecule in the hydrolysis of the beta-D-mannosidic bond
-
E265A
-
0.04% of wild-type activity. Glutamic acid residues E265 and E224 are critical for the hydrolysis of beta-1,2-mannotriose. The residues are not conserved among GH130 phosphorylases and are predicted to assist the nucleophilic attack of a water molecule in the hydrolysis of the beta-D-mannosidic bond
-
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Nihira, T.; Chiku, K.; Suzuki, E.; Nishimoto, M.; Fushinobu, S.; Kitaoka, M.; Ohtsubo, K.; Nakai, H.
An inverting beta-1,2-mannosidase belonging to glycoside hydrolase family 130 from Dyadobacter fermentans
FEBS Lett.
589
3604-3610
2015
Dyadobacter fermentans (C6W7B1), Dyadobacter fermentans, Dyadobacter fermentans DSM 18053 (C6W7B1)
brenda
Cuskin, F.; Basle, A.; Ladeveze, S.; Day, A.; Gilbert, H.; Davies, G.; Potocki-Veronese, G.; Lowe, E.
The GH130 family of mannoside phosphorylases contains glycoside hydrolases that target beta-1,2-mannosidic linkages in Candida mannan
J. Biol. Chem.
290
25023-25033
2015
Bacteroides ovatus (A7M0J4), Bacteroides thetaiotaomicron (Q8A186), Bacteroides thetaiotaomicron DSM 2079 (Q8A186), Bacteroides ovatus DSM 1896 (A7M0J4)
brenda