EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    3.2.1.4 | -999 |
- |
- |
326319, 393552, 393560, 393564, 393569, 393578, 393589, 393604, 393607, 393613, 393615, 393619, 393634, 393637, 393641, 655827, 666469 |
| 3.2.1.4 | -999 |
- |
2.82 U/ml for the native extracellular enzyme, 6.78-8.54 U/ml for the recombinant extracellular enzyme expressed in Escherichia coli strain BL21(DE3) |
709882 |
| 3.2.1.4 | -999 |
- |
assay methods |
393586 |
| 3.2.1.4 | -999 |
- |
discontinuous test based on the specific determination of the cellobiose produced during the cellulase reaction with an ancillary cellobiose dehydrogenase |
393585 |
| 3.2.1.4 | -999 |
- |
pH 5.0, specific activity of the enzyme recombinantly expressed in Sulfolobus solfataricus is about 200fold higher compared to the specific activity of the enzyme recombinantly expressed in Escherichia coli |
722548 |
| 3.2.1.4 | -999 |
- |
purified native enzyme, 1289.83 U/ml, pH 5.0, 50°C |
750083 |
| 3.2.1.4 | -999 |
- |
recombinant crude enzyme, 5.772 U/ml |
751987 |
| 3.2.1.4 | -999 |
- |
staining techniques for the detection |
393570 |
| 3.2.1.4 | -999 |
- |
substrate carboxymethyl cellulose, recombinant wild-type enzyme shows 70.4 mU/ml activity, activities of enzyme mutants G263C/R307H, G145D/N207K, P228R, T67N/D142E/S218N/V242D/D330E, and T157I/G251D/V259D are 103.8, 88.6, 112.6, 89.1, and 102.9 mU/ml, respectively |
749552 |
| 3.2.1.4 | 0.018 |
- |
TfCel9A wild-type, activity on 600 microM G5 |
690958 |
